Plant Circular RNA Database

Detail information of Os04g0648800_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os04g0648800_circ_g.5
					
						ID in PlantcircBase
						osa_circ_025656
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 33026698-33027492  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os04g0648800						
					
						Parent gene annotation
						
							C3HC4 RING domain-containing E3 ubiquitin ligase, Modulation of 
heading date by physically interacting with Hd1 (Os04t0648800-01
);Similar to Zinc finger, C3HC4 type family protein. (Os04t06488
00-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os04g0648800_circ_g.6 Os04g0648800_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0648775-00:3
Os04t0648775-00:3
Os04t0648800-02:3
Os04t0648800-01:3

General Information
CircRNA Name	Os04g0648800_circ_g.5
ID in PlantcircBase	osa_circ_025656
Alias	NA
Organism	Oryza sativa
Position	chr4: 33026698-33027492 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer
Parent gene	Os04g0648800
Parent gene annotation	C3HC4 RING domain-containing E3 ubiquitin ligase, Modulation of heading date by physically interacting with Hd1 (Os04t0648800-01 );Similar to Zinc finger, C3HC4 type family protein. (Os04t06488 00-02)
Parent gene strand	+
Alternative splicing	Os04g0648800_circ_g.6 Os04g0648800_circ_g.7
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0648775-00:3 Os04t0648775-00:3 Os04t0648800-02:3 Os04t0648800-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCTCAGTGAGGAAGAAGTGACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAA
GCATCTGTGGAAGAAGAGCCATGCTGTATCTGCCAGatccggaacgcgcttgaaatgattcaca
gaggggaaaatgtaagatttgaggtcagtaacaactgtggtttcggagttacctacagttgcct
atttttat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 273
Transcript exons: 33026698-33026748,33027198-33027275,33027349-33027492
ATCCGGAACGCGCTTGAAATGATTCACAGAGGGGAAAATGTAAGATTTGAGTCCATTTTCTATG
GTGGAGTCGACATTCATGACAGACACAGGGATATGCGCCTTGACATAGACAACATGTCTTATGA
GGAGCTATTGGCACTGGAAGAAAGAATAGGCAATGTTAGCACTGGCCTCAGTGAGGAAGAAGTG
ACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAAGCATCTGTGGAAGAAGAGC
CATGCTGTATCTGCCAG
						
						Genomic sequence
						
							ATCCGGAACGCGCTTGAAATGATTCACAGAGGGGAAAATGTAAGATTTGAGGTCAGTAACAACT
GTGGTTTCGGAGTTACCTACAGTTGCCTATTTTTATCTGCACGGACTTGTAGAGATTGATGAAA
CTATATTATGTCCTAAAAACTCACAGCAAGACTGGCTGACTAATACTAGTTGATGCTAGGATTA
CATAAGGTTTGCTCTGTCCATTCAGTTTGGATGTAGAGGCTGGATTCTAATCCATTTTATAAAA
GAGAGATCGCATAAGGTGTTGTTTTGTTTCTGAAAACAGAAGTTTCTTGTAATATGGAAATAGT
ATATAGTGAATGCATGAAAGACCATAGTATATCTATAATTCAGAAAAATATAGTCATCATTTCA
ACAGAGAGAAACAAATAGGCAACAACTTGCACTGAACCCTTTTTTGTTTTCTAAAATCTTTGCT
TATTTCTTGAGGTATTTACTCTTGCTTCCACGTATATCTGTTCTTGCTACAGTCCATTTTCTAT
GGTGGAGTCGACATTCATGACAGACACAGGGATATGCGCCTTGACATAGACAACATGTCTTATG
AGGTTGGTTTTCCTTAGCACAATATTATCTGGATCTTTCTCCAGCAAAACTCTGATAATTGCTG
TTGTCTTCCAGGAGCTATTGGCACTGGAAGAAAGAATAGGCAATGTTAGCACTGGCCTCAGTGA
GGAAGAAGTGACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAAGCATCTGTG
GAAGAAGAGCCATGCTGTATCTGCCAG

Sequences
Splice junction sequence	CCTCAGTGAGGAAGAAGTGACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAA GCATCTGTGGAAGAAGAGCCATGCTGTATCTGCCAGatccggaacgcgcttgaaatgattcaca gaggggaaaatgtaagatttgaggtcagtaacaactgtggtttcggagttacctacagttgcct atttttat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 273 Transcript exons: 33026698-33026748,33027198-33027275,33027349-33027492 ATCCGGAACGCGCTTGAAATGATTCACAGAGGGGAAAATGTAAGATTTGAGTCCATTTTCTATG GTGGAGTCGACATTCATGACAGACACAGGGATATGCGCCTTGACATAGACAACATGTCTTATGA GGAGCTATTGGCACTGGAAGAAAGAATAGGCAATGTTAGCACTGGCCTCAGTGAGGAAGAAGTG ACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAAGCATCTGTGGAAGAAGAGC CATGCTGTATCTGCCAG
Genomic sequence	ATCCGGAACGCGCTTGAAATGATTCACAGAGGGGAAAATGTAAGATTTGAGGTCAGTAACAACT GTGGTTTCGGAGTTACCTACAGTTGCCTATTTTTATCTGCACGGACTTGTAGAGATTGATGAAA CTATATTATGTCCTAAAAACTCACAGCAAGACTGGCTGACTAATACTAGTTGATGCTAGGATTA CATAAGGTTTGCTCTGTCCATTCAGTTTGGATGTAGAGGCTGGATTCTAATCCATTTTATAAAA GAGAGATCGCATAAGGTGTTGTTTTGTTTCTGAAAACAGAAGTTTCTTGTAATATGGAAATAGT ATATAGTGAATGCATGAAAGACCATAGTATATCTATAATTCAGAAAAATATAGTCATCATTTCA ACAGAGAGAAACAAATAGGCAACAACTTGCACTGAACCCTTTTTTGTTTTCTAAAATCTTTGCT TATTTCTTGAGGTATTTACTCTTGCTTCCACGTATATCTGTTCTTGCTACAGTCCATTTTCTAT GGTGGAGTCGACATTCATGACAGACACAGGGATATGCGCCTTGACATAGACAACATGTCTTATG AGGTTGGTTTTCCTTAGCACAATATTATCTGGATCTTTCTCCAGCAAAACTCTGATAATTGCTG TTGTCTTCCAGGAGCTATTGGCACTGGAAGAAAGAATAGGCAATGTTAGCACTGGCCTCAGTGA GGAAGAAGTGACAAAGCTCCTAAAGCAAAGGAAATTCTCATCATGGAGGTTGGAAGCATCTGTG GAAGAAGAGCCATGCTGTATCTGCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.233836457

Conservation Information
Conserved circRNAs	NA
PMCS	0.233836457

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							33026717-33027489(+)
33027445-33026711(+)
33027229-33027444(-)
						
						Potential amino acid sequence
						
							MIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQ
RKFSSWRLEASVEEEPCCICQIRNALEMIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYE
ELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQIRNALEMIHRGENVRF
ESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEA
SVEEEPCCICQIRNALEMIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIG
NVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQ(+)
MEVGSICGRRAMLYLPDPERA*(+)
MNVDSTIENGLKSYIFPSVNHFKRVPDLADTAWLFFHRCFQPP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	33026717-33027489(+) 33027445-33026711(+) 33027229-33027444(-)
Potential amino acid sequence	MIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQ RKFSSWRLEASVEEEPCCICQIRNALEMIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYE ELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQIRNALEMIHRGENVRF ESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEA SVEEEPCCICQIRNALEMIHRGENVRFESIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIG NVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQ(+) MEVGSICGRRAMLYLPDPERA(+) MNVDSTIENGLKSYIFPSVNHFKRVPDLADTAWLFFHRCFQPP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os04g0648800_circ_g.5

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