Plant Circular RNA Database

Detail information of Os01g0973300_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os01g0973300_circ_g.2
					
						ID in PlantcircBase
						osa_circ_006029
					
						Alias
						Os_ciR6502
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 42974349-42975608  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os01g0973300						
					
						Parent gene annotation
						
							Armadillo-like helical domain containing protein. (Os01t0973300-
01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0973300_circ_g.3 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0973300-01:2

General Information
CircRNA Name	Os01g0973300_circ_g.2
ID in PlantcircBase	osa_circ_006029
Alias	Os_ciR6502
Organism	Oryza sativa
Position	chr1: 42974349-42975608 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os01g0973300
Parent gene annotation	Armadillo-like helical domain containing protein. (Os01t0973300- 01)
Parent gene strand	+
Alternative splicing	Os01g0973300_circ_g.3
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0973300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGAATGTTTCAACAAACCAACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCTGGTAGTGA
GGATGGCTTGGACCAAACATTACAAGGATCTGCAAGgtgattgtacgcttggtccacagcctgg
acaaaattaaggagcctgcagcacgatccctgattatttggatatttggagagtatagttccat
aggaaata

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1169
Transcript exons: 42974349-42974549,42974641-42975608
GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA
TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGATAACTCCTGCTGTTCT
CAAGTATCTTGCATGGTCCTTTGCTGCTGAAATGCTTGAGACAAAGCTTCAGATCTTAAATGCT
GCTGCAAAGGTTATCATACATTCTCCTGAAGAACACTTAGAAGAATTCAAAAAGATAATGGCCC
ATGTCATTAAGCTGGCTACATGTGATTTGAGTTATGACGTCCGTGATCGTGCCCGTTTTATATC
AAGACTTTTGCCATACAGCACAACCTATTTAAATGGAAACAACTCCTCATGTCAATCACATAAT
GAAGATATGTTTAAGGAACTGGCTAATCATATATTTGATGGGAAGATGCCATCAACATTCCATC
CAACCAATAACTACCGGATTTATCTTCCTGGTTCCTTGTCACAGGTGATTCTTCATGCAGCTCC
TGGTTACGCACCACTTCCAAAGCCTCAGAGTATGGAATTAATTCATAAGACCATGGAACCAACC
AGAGGAGTAGGCAATTCTTCTGAAAGCATTAATTCTGATGCTGAATCTGGATCTTCAACATATG
ATGGTGGTTCTGTTTATGATTCAGAGAGTGAAGTTGATGGCTCATCTGACAGGAATGCTGCTGA
TTCTAACACAAAAGATAATCAAGAAGATCCCCTGGTCCATGTTTATGATGCAAGTGTAGATCAA
GGTCAGACGGCTAGGGATGTCGAGGATAATTTTGCATCTCTCATCACTACTGATTTGACTGAAT
TAATGTCTAAGTCAGCCTTGGAAACATGGCTTGATGAAGCTCCTGCTGAACCAGTTCAGGTTTC
TACACAAGCATCCTCTGCTAGAGTGTCTTTTACCAATCGGAGCTTTGAGCGAAAACCTAAATTG
CACATGTTGTTAGACCCATCTAATAGTAATGGTCTAAGTGTTCTTTATGCCTTTTCATCTGAGG
TTTCACCCGTATCACGCTTGCTAGTGTGTGTAGACTTGTTATTTGAGAATGTTTCAACAAACCA
ACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCTGGTAGTGAGGATGGCTTGGACCAAACA
TTACAAGGATCTGCAAG
Transcript length: 169
Transcript exons: 42974349-42974459,42975551-42975608
GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA
TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGCCGAGGAGGCTTCTGGT
AGTGAGGATGGCTTGGACCAAACATTACAAGGATCTGCAAG
						
					

						Genomic sequence
						
							GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA
TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGATAACTCCTGCTGTTCT
CAAGTATCTTGCATGGTCCTTTGCTGCTGAAATGCTTGAGACAAAGCTTCAGATCTTAAATGCT
GCTGCAAAGGTCCCTCCATCCTCCATCTCTCCCTCTCTGTAAGAATTCAAAAGGTTCCTTTTCT
CTTCTGTGGGGATCACTAATTGTTCTTCCTTTACAGGTTATCATACATTCTCCTGAAGAACACT
TAGAAGAATTCAAAAAGATAATGGCCCATGTCATTAAGCTGGCTACATGTGATTTGAGTTATGA
CGTCCGTGATCGTGCCCGTTTTATATCAAGACTTTTGCCATACAGCACAACCTATTTAAATGGA
AACAACTCCTCATGTCAATCACATAATGAAGATATGTTTAAGGAACTGGCTAATCATATATTTG
ATGGGAAGATGCCATCAACATTCCATCCAACCAATAACTACCGGATTTATCTTCCTGGTTCCTT
GTCACAGGTGATTCTTCATGCAGCTCCTGGTTACGCACCACTTCCAAAGCCTCAGAGTATGGAA
TTAATTCATAAGACCATGGAACCAACCAGAGGAGTAGGCAATTCTTCTGAAAGCATTAATTCTG
ATGCTGAATCTGGATCTTCAACATATGATGGTGGTTCTGTTTATGATTCAGAGAGTGAAGTTGA
TGGCTCATCTGACAGGAATGCTGCTGATTCTAACACAAAAGATAATCAAGAAGATCCCCTGGTC
CATGTTTATGATGCAAGTGTAGATCAAGGTCAGACGGCTAGGGATGTCGAGGATAATTTTGCAT
CTCTCATCACTACTGATTTGACTGAATTAATGTCTAAGTCAGCCTTGGAAACATGGCTTGATGA
AGCTCCTGCTGAACCAGTTCAGGTTTCTACACAAGCATCCTCTGCTAGAGTGTCTTTTACCAAT
CGGAGCTTTGAGCGAAAACCTAAATTGCACATGTTGTTAGACCCATCTAATAGTAATGGTCTAA
GTGTTCTTTATGCCTTTTCATCTGAGGTTTCACCCGTATCACGCTTGCTAGTGTGTGTAGACTT
GTTATTTGAGAATGTTTCAACAAACCAACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCT
GGTAGTGAGGATGGCTTGGACCAAACATTACAAGGATCTGCAAG
						
					

			
		

Sequences
Splice junction sequence	AGAATGTTTCAACAAACCAACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCTGGTAGTGA GGATGGCTTGGACCAAACATTACAAGGATCTGCAAGgtgattgtacgcttggtccacagcctgg acaaaattaaggagcctgcagcacgatccctgattatttggatatttggagagtatagttccat aggaaata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1169 Transcript exons: 42974349-42974549,42974641-42975608 GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGATAACTCCTGCTGTTCT CAAGTATCTTGCATGGTCCTTTGCTGCTGAAATGCTTGAGACAAAGCTTCAGATCTTAAATGCT GCTGCAAAGGTTATCATACATTCTCCTGAAGAACACTTAGAAGAATTCAAAAAGATAATGGCCC ATGTCATTAAGCTGGCTACATGTGATTTGAGTTATGACGTCCGTGATCGTGCCCGTTTTATATC AAGACTTTTGCCATACAGCACAACCTATTTAAATGGAAACAACTCCTCATGTCAATCACATAAT GAAGATATGTTTAAGGAACTGGCTAATCATATATTTGATGGGAAGATGCCATCAACATTCCATC CAACCAATAACTACCGGATTTATCTTCCTGGTTCCTTGTCACAGGTGATTCTTCATGCAGCTCC TGGTTACGCACCACTTCCAAAGCCTCAGAGTATGGAATTAATTCATAAGACCATGGAACCAACC AGAGGAGTAGGCAATTCTTCTGAAAGCATTAATTCTGATGCTGAATCTGGATCTTCAACATATG ATGGTGGTTCTGTTTATGATTCAGAGAGTGAAGTTGATGGCTCATCTGACAGGAATGCTGCTGA TTCTAACACAAAAGATAATCAAGAAGATCCCCTGGTCCATGTTTATGATGCAAGTGTAGATCAA GGTCAGACGGCTAGGGATGTCGAGGATAATTTTGCATCTCTCATCACTACTGATTTGACTGAAT TAATGTCTAAGTCAGCCTTGGAAACATGGCTTGATGAAGCTCCTGCTGAACCAGTTCAGGTTTC TACACAAGCATCCTCTGCTAGAGTGTCTTTTACCAATCGGAGCTTTGAGCGAAAACCTAAATTG CACATGTTGTTAGACCCATCTAATAGTAATGGTCTAAGTGTTCTTTATGCCTTTTCATCTGAGG TTTCACCCGTATCACGCTTGCTAGTGTGTGTAGACTTGTTATTTGAGAATGTTTCAACAAACCA ACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCTGGTAGTGAGGATGGCTTGGACCAAACA TTACAAGGATCTGCAAG Transcript length: 169 Transcript exons: 42974349-42974459,42975551-42975608 GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGCCGAGGAGGCTTCTGGT AGTGAGGATGGCTTGGACCAAACATTACAAGGATCTGCAAG
Genomic sequence	GTGATTGTACGCTTGGTCCACAGCCTGGACAAAATTAAGGAGCCTGCAGCACGATCCCTGATTA TTTGGATATTTGGAGAGTATAGTTCCATAGGAAATATCATTCCAAAGATAACTCCTGCTGTTCT CAAGTATCTTGCATGGTCCTTTGCTGCTGAAATGCTTGAGACAAAGCTTCAGATCTTAAATGCT GCTGCAAAGGTCCCTCCATCCTCCATCTCTCCCTCTCTGTAAGAATTCAAAAGGTTCCTTTTCT CTTCTGTGGGGATCACTAATTGTTCTTCCTTTACAGGTTATCATACATTCTCCTGAAGAACACT TAGAAGAATTCAAAAAGATAATGGCCCATGTCATTAAGCTGGCTACATGTGATTTGAGTTATGA CGTCCGTGATCGTGCCCGTTTTATATCAAGACTTTTGCCATACAGCACAACCTATTTAAATGGA AACAACTCCTCATGTCAATCACATAATGAAGATATGTTTAAGGAACTGGCTAATCATATATTTG ATGGGAAGATGCCATCAACATTCCATCCAACCAATAACTACCGGATTTATCTTCCTGGTTCCTT GTCACAGGTGATTCTTCATGCAGCTCCTGGTTACGCACCACTTCCAAAGCCTCAGAGTATGGAA TTAATTCATAAGACCATGGAACCAACCAGAGGAGTAGGCAATTCTTCTGAAAGCATTAATTCTG ATGCTGAATCTGGATCTTCAACATATGATGGTGGTTCTGTTTATGATTCAGAGAGTGAAGTTGA TGGCTCATCTGACAGGAATGCTGCTGATTCTAACACAAAAGATAATCAAGAAGATCCCCTGGTC CATGTTTATGATGCAAGTGTAGATCAAGGTCAGACGGCTAGGGATGTCGAGGATAATTTTGCAT CTCTCATCACTACTGATTTGACTGAATTAATGTCTAAGTCAGCCTTGGAAACATGGCTTGATGA AGCTCCTGCTGAACCAGTTCAGGTTTCTACACAAGCATCCTCTGCTAGAGTGTCTTTTACCAAT CGGAGCTTTGAGCGAAAACCTAAATTGCACATGTTGTTAGACCCATCTAATAGTAATGGTCTAA GTGTTCTTTATGCCTTTTCATCTGAGGTTTCACCCGTATCACGCTTGCTAGTGTGTGTAGACTT GTTATTTGAGAATGTTTCAACAAACCAACTGGCAGATATAACAATTAAATCCGAGGAGGCTTCT GGTAGTGAGGATGGCTTGGACCAAACATTACAAGGATCTGCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.312707116

Conservation Information
Conserved circRNAs	NA
PMCS	0.312707116

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							42974509-42974349(+)
42975576-42974383(+)
42974454-42975547(-)
						
						Potential amino acid sequence
						
							MLETKLQILNAAAKVIIHSPEEHLEEFKKIMAHVIKLATCDLSYDVRDRARFISRLLPYSTTYL
NGNNSSCQSHNEDMFKELANHIFDGKMPSTFHPTNNYRIYLPGSLSQVILHAAPGYAPLPKPQS
MELIHKTMEPTRGVGNSSESINSDAESGSSTYDGGSVYDSESEVDGSSDRNAADSNTKDNQEDP
LVHVYDASVDQGQTARDVEDNFASLITTDLTELMSKSALETWLDEAPAEPVQVSTQASSARVSF
TNRSFERKPKLHMLLDPSNSNGLSVLYAFSSEVSPVSRLLVCVDLLFENVSTNQLADITIKSEE
ASGSEDGLDQTLQGSAR*(+)
MAWTKHYKDLQGDCTLGPQPGQN*(+)
MIFPMELYSPNIQIIRDRAAGSLILSRLWTKRTITLQILVMFGPSHPHYQKPPRI*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	42974509-42974349(+) 42975576-42974383(+) 42974454-42975547(-)
Potential amino acid sequence	MLETKLQILNAAAKVIIHSPEEHLEEFKKIMAHVIKLATCDLSYDVRDRARFISRLLPYSTTYL NGNNSSCQSHNEDMFKELANHIFDGKMPSTFHPTNNYRIYLPGSLSQVILHAAPGYAPLPKPQS MELIHKTMEPTRGVGNSSESINSDAESGSSTYDGGSVYDSESEVDGSSDRNAADSNTKDNQEDP LVHVYDASVDQGQTARDVEDNFASLITTDLTELMSKSALETWLDEAPAEPVQVSTQASSARVSF TNRSFERKPKLHMLLDPSNSNGLSVLYAFSSEVSPVSRLLVCVDLLFENVSTNQLADITIKSEE ASGSEDGLDQTLQGSAR(+) MAWTKHYKDLQGDCTLGPQPGQN(+) MIFPMELYSPNIQIIRDRAAGSLILSRLWTKRTITLQILVMFGPSHPHYQKPPRI*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os01g0973300_circ_g.2

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