Plant Circular RNA Database

Detail information of HORVU4Hr1G073320_circ_g.1

			
						 General Information
					
						CircRNA Name
						HORVU4Hr1G073320_circ_g.1
					
						ID in PlantcircBase
						hvu_circ_000026
					
						Alias
						NA
					
						Organism
						
							Hordeum vulgare						
					
						Position
						
							chrchr4H: 588905852-588906207  JBrowse»						
					
						Reference genome
						
							Hv_IBSC_PGSB_v2.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							HORVU4Hr1G073320						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaves, seeds
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							AC-AA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						HORVU4Hr1G073320.3:1
HORVU4Hr1G073320.1:1
HORVU4Hr1G073320.2:1
HORVU4Hr1G073320.4:1

General Information
CircRNA Name	HORVU4Hr1G073320_circ_g.1
ID in PlantcircBase	hvu_circ_000026
Alias	NA
Organism	Hordeum vulgare
Position	chrchr4H: 588905852-588906207 JBrowse»
Reference genome	Hv_IBSC_PGSB_v2.38
Type	u-circRNA
Identification method	CIRI
Parent gene	HORVU4Hr1G073320
Parent gene annotation	protein_coding
Parent gene strand	-
Alternative splicing	NA
Support reads	NA
Tissues	leaves, seeds
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	AC-AA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	HORVU4Hr1G073320.3:1 HORVU4Hr1G073320.1:1 HORVU4Hr1G073320.2:1 HORVU4Hr1G073320.4:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: ACCTATATGGTCCTGACTTAGGTATTTGT
 R: GAATCTAGGTGGAAAGCTGCTATTCT 
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: ACCTATATGGTCCTGACTTAGGTATTTGT R: GAATCTAGGTGGAAAGCTGCTATTCT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCTTCACGGCAGCTTCCCGTGAGTCATGATGTGCTTGCTAACCAACCTAACAGTACTCCTATTG
ATGCAGAATCTAGGTGGAAAGCTGCTATTCTAAGTTcagatgccggagtgtaaactccagtcat
ctaacagtagaacagaacttaccaccctacccctgcctgatgcagtttcctactagctaggatc
atgcagac

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CAGATGCCGGAGTGTAAACTCCAGTCATCTAACAGTAGAACAGAACTTACCACCCTACCCCTGC
CTGATGCAGTTTCCTACTAGCTAGGATCATGCAGACTACCAACCACTAATGGATCACTGACCAT
GGATGGAAACGAAGATCTAACACCTAAAGAACAAATACCTAAGTCAGGACCATATAGGTGACCA
CAAGCTACAAGGTGACTCTGAAACTGCTGTGCTCTCGTCCACCCCACAGTCTCCACCACCACAT
CCTTCACGGCAGCTTCCCGTGAGTCATGATGTGCTTGCTAACCAACCTAACAGTACTCCTATTG
ATGCAGAATCTAGGTGGAAAGCTGCTATTCTAAGTT

Sequences
Splice junction sequence	CCTTCACGGCAGCTTCCCGTGAGTCATGATGTGCTTGCTAACCAACCTAACAGTACTCCTATTG ATGCAGAATCTAGGTGGAAAGCTGCTATTCTAAGTTcagatgccggagtgtaaactccagtcat ctaacagtagaacagaacttaccaccctacccctgcctgatgcagtttcctactagctaggatc atgcagac
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CAGATGCCGGAGTGTAAACTCCAGTCATCTAACAGTAGAACAGAACTTACCACCCTACCCCTGC CTGATGCAGTTTCCTACTAGCTAGGATCATGCAGACTACCAACCACTAATGGATCACTGACCAT GGATGGAAACGAAGATCTAACACCTAAAGAACAAATACCTAAGTCAGGACCATATAGGTGACCA CAAGCTACAAGGTGACTCTGAAACTGCTGTGCTCTCGTCCACCCCACAGTCTCCACCACCACAT CCTTCACGGCAGCTTCCCGTGAGTCATGATGTGCTTGCTAACCAACCTAACAGTACTCCTATTG ATGCAGAATCTAGGTGGAAAGCTGCTATTCTAAGTT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.172491807

Conservation Information
Conserved circRNAs	NA
PMCS	0.172491807

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Darbani et al., 2016

circRNA

Detail information of HORVU4Hr1G073320_circ_g.1

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