Plant Circular RNA Database

Detail information of GLYMA_20G004000_circ_g.7

			
						 General Information
					
						CircRNA Name
						GLYMA_20G004000_circ_g.7
					
						ID in PlantcircBase
						gma_circ_005108
					
						Alias
						Gm20circRNA442, gma-circRNA2770
					
						Organism
						
							Glycine max						
					
						Position
						
							chr20: 390527-391464  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Tophat, CIRI, find_circ
					
						Parent gene
						
							GLYMA_20G004000						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							GLYMA_20G004000_circ_g.1 GLYMA_20G004000_circ_g.2 GLYMA_20G004000_circ_g.3 GLYMA_20G004000_circ_g.4 GLYMA_20G004000_circ_g.5 GLYMA_20G004000_circ_g.6 GLYMA_20G004000_circ_g.8 						
					
						Support reads
						NA
					
						Tissues
						leaf, root, stem, flower bud
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRG89143:3
KRG89144:3
KRG89142:3

General Information
CircRNA Name	GLYMA_20G004000_circ_g.7
ID in PlantcircBase	gma_circ_005108
Alias	Gm20circRNA442, gma-circRNA2770
Organism	Glycine max
Position	chr20: 390527-391464 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	Tophat, CIRI, find_circ
Parent gene	GLYMA_20G004000
Parent gene annotation	hypothetical protein
Parent gene strand	+
Alternative splicing	GLYMA_20G004000_circ_g.1 GLYMA_20G004000_circ_g.2 GLYMA_20G004000_circ_g.3 GLYMA_20G004000_circ_g.4 GLYMA_20G004000_circ_g.5 GLYMA_20G004000_circ_g.6 GLYMA_20G004000_circ_g.8
Support reads	NA
Tissues	leaf, root, stem, flower bud
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRG89143:3 KRG89144:3 KRG89142:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATGTGGAAAAATTGTTGATGTTCGTCTACATCGGAATCACAATGGTAGGTTAAATGGCTTTGGA
CAAGTTGAGTTTGCAACAGCAGAAGCTGCGAAAAAAcaactggagcaagtcattccaccagagt
gaacaaactaagcccatcaaaagtacagagagttcagagtactgtgaggaagaggttgagggaa
aagcctcc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 328
Transcript exons: 390527-390644,391181-391272,391347-391464
CAACTGGAGCAAGTCATTCCACCAGAGTGAACAAACTAAGCCCATCAAAAGTACAGAGAGTTCA
GAGTACTGTGAGGAAGAGGTTGAGGGAAAAGCCTCCAATAGTTATTGGGAAAGTTCTAAAAGGG
TAGCTACTCTGAAAGAACAAAATGCTCCACCAAAGACAATATATGTCAGAAACTTGTCATACAG
AGTGGAACAGGCTGATATGGAAAATCTTTTCAAAGAATGTGGAAAAATTGTTGATGTTCGTCTA
CATCGGAATCACAATGGTAGGTTAAATGGCTTTGGACAAGTTGAGTTTGCAACAGCAGAAGCTG
CGAAAAAA
						
						Genomic sequence
						
							CAACTGGAGCAAGTCATTCCACCAGAGTGAACAAACTAAGCCCATCAAAAGTACAGAGAGTTCA
GAGTACTGTGAGGAAGAGGTTGAGGGAAAAGCCTCCAATAGTTATTGGGAAAGTGTAAGCATTT
CATTATGCATTTGCTTATATGAATTATTGGAGTATATAGTTGTCTTGTGTGAAATGCTTAGGGA
TGTGGAGATGGTTGGTGCTTCCTCCATTCTGTGAGGGGTTTGGATCGAATGTGAATTATAGTTC
AAGATGTGGGTGGTGGATAGCCTTAAACATTTCTATATGGCAGCATAGGAATCAATTGATTTTC
CAAGGAAAACATTTTGATCCCTATAAAGTCATGGATCATGCTATTTACTTAGCTTGGTCATGGT
TAAAGGCTTATGATAAGGATTTCAGCATTAATTTCCTTCACTGGTCTTCTAATATATCTACTTT
TGTTGATTAGATGGGTTTGGGTCTGAATTTTTATGTTGAGTTTGTGTGTTGTTTTGGAGGGTAG
CACTCTGGTGCCTTGTAATTCTGCTCTTTGGTACCTCTGGTACTGGCTTACCTATTAATGATAT
ATATATATATTTGCAGCCTTCCAAAAAAAAAAAAGATCAAATCAGTTATATTCTATGTTATTTC
CTGGAATTTTGCAGTCTAAAAGGGTAGCTACTCTGAAAGAACAAAATGCTCCACCAAAGACAAT
ATATGTCAGAAACTTGTCATACAGAGTGGAACAGGCTGATATGTATGTAATATTTTTCATGCAA
TTACTTGTTGATTATTTTCTGTACATTTTTTTATAATTTCAAAAAATTTCAGGGAAAATCTTTT
CAAAGAATGTGGAAAAATTGTTGATGTTCGTCTACATCGGAATCACAATGGTAGGTTAAATGGC
TTTGGACAAGTTGAGTTTGCAACAGCAGAAGCTGCGAAAAAA

Sequences
Splice junction sequence	ATGTGGAAAAATTGTTGATGTTCGTCTACATCGGAATCACAATGGTAGGTTAAATGGCTTTGGA CAAGTTGAGTTTGCAACAGCAGAAGCTGCGAAAAAAcaactggagcaagtcattccaccagagt gaacaaactaagcccatcaaaagtacagagagttcagagtactgtgaggaagaggttgagggaa aagcctcc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 328 Transcript exons: 390527-390644,391181-391272,391347-391464 CAACTGGAGCAAGTCATTCCACCAGAGTGAACAAACTAAGCCCATCAAAAGTACAGAGAGTTCA GAGTACTGTGAGGAAGAGGTTGAGGGAAAAGCCTCCAATAGTTATTGGGAAAGTTCTAAAAGGG TAGCTACTCTGAAAGAACAAAATGCTCCACCAAAGACAATATATGTCAGAAACTTGTCATACAG AGTGGAACAGGCTGATATGGAAAATCTTTTCAAAGAATGTGGAAAAATTGTTGATGTTCGTCTA CATCGGAATCACAATGGTAGGTTAAATGGCTTTGGACAAGTTGAGTTTGCAACAGCAGAAGCTG CGAAAAAA
Genomic sequence	CAACTGGAGCAAGTCATTCCACCAGAGTGAACAAACTAAGCCCATCAAAAGTACAGAGAGTTCA GAGTACTGTGAGGAAGAGGTTGAGGGAAAAGCCTCCAATAGTTATTGGGAAAGTGTAAGCATTT CATTATGCATTTGCTTATATGAATTATTGGAGTATATAGTTGTCTTGTGTGAAATGCTTAGGGA TGTGGAGATGGTTGGTGCTTCCTCCATTCTGTGAGGGGTTTGGATCGAATGTGAATTATAGTTC AAGATGTGGGTGGTGGATAGCCTTAAACATTTCTATATGGCAGCATAGGAATCAATTGATTTTC CAAGGAAAACATTTTGATCCCTATAAAGTCATGGATCATGCTATTTACTTAGCTTGGTCATGGT TAAAGGCTTATGATAAGGATTTCAGCATTAATTTCCTTCACTGGTCTTCTAATATATCTACTTT TGTTGATTAGATGGGTTTGGGTCTGAATTTTTATGTTGAGTTTGTGTGTTGTTTTGGAGGGTAG CACTCTGGTGCCTTGTAATTCTGCTCTTTGGTACCTCTGGTACTGGCTTACCTATTAATGATAT ATATATATATTTGCAGCCTTCCAAAAAAAAAAAAGATCAAATCAGTTATATTCTATGTTATTTC CTGGAATTTTGCAGTCTAAAAGGGTAGCTACTCTGAAAGAACAAAATGCTCCACCAAAGACAAT ATATGTCAGAAACTTGTCATACAGAGTGGAACAGGCTGATATGTATGTAATATTTTTCATGCAA TTACTTGTTGATTATTTTCTGTACATTTTTTTATAATTTCAAAAAATTTCAGGGAAAATCTTTT CAAAGAATGTGGAAAAATTGTTGATGTTCGTCTACATCGGAATCACAATGGTAGGTTAAATGGC TTTGGACAAGTTGAGTTTGCAACAGCAGAAGCTGCGAAAAAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.206749769

Conservation Information
Conserved circRNAs	NA
PMCS	0.206749769

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							391272-390553(+)
391419-390553(+)
390573-391416(-)
390545-391403(-)
						
						Potential amino acid sequence
						
							MENLFKECGKIVDVRLHRNHNGRLNGFGQVEFATAEAAKKQLEQVIPPE*(+)
MALDKLSLQQQKLRKNNWSKSFHQSEQTKPIKSTESSEYCEEEVEGKASNSYWESSKRVATLKE
QNAPPKTIYVRNLSYRVEQADMENLFKECGKIVDVRLHRNHNGRLNGFGQVEFATAEAAKKQLE
QVIPPE*(+)
MGLVCSLWWNDLLQLFFRSFCCCKLNLSKAI*(-)
MTCSSCFFAASAVANSTCPKPFNLPL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						cytoplasmic male sterility

Functional Information
Coding potential	Y
Potential coding position	391272-390553(+) 391419-390553(+) 390573-391416(-) 390545-391403(-)
Potential amino acid sequence	MENLFKECGKIVDVRLHRNHNGRLNGFGQVEFATAEAAKKQLEQVIPPE(+) MALDKLSLQQQKLRKNNWSKSFHQSEQTKPIKSTESSEYCEEEVEGKASNSYWESSKRVATLKE QNAPPKTIYVRNLSYRVEQADMENLFKECGKIVDVRLHRNHNGRLNGFGQVEFATAEAAKKQLE QVIPPE(+) MGLVCSLWWNDLLQLFFRSFCCCKLNLSKAI(-) MTCSSCFFAASAVANSTCPKPFNLPL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	cytoplasmic male sterility

			
						 Other Information
					
						References
						Zhao et al., 2017a; Chen et al., 2018

circRNA

Detail information of GLYMA_20G004000_circ_g.7

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