Plant Circular RNA Database

Detail information of AT5G09590_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT5G09590_circ_g.4
					
						ID in PlantcircBase
						ath_circ_037264
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 2976315-2977592  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT5G09590						
					
						Parent gene annotation
						
							Heat shock 70 kDa protein 10, mitochondrial
							
						Parent gene strand
						+
					
						Alternative splicing
						
							5_circ_ag.29 AT5G09570_circ_g.1 AT5G09570_circ_g.2 AT5G09570_circ_g.3 AT5G09580_circ_g.1 AT5G09590_circ_g.1 AT5G09590_circ_g.2 AT5G09590_circ_g.3 AT5G09590_circ_g.5 AT5G09590_circ_g.6 AT5G09620_circ_g.1 AT5G09650_circ_g.1 AT5G09650_circ_g.2 AT5G09660_circ_g.1 AT5G09660_circ_g.2 AT5G09660_circ_g.3 AT5G09660_circ_g.4 AT5G09660_circ_g.5 AT5G09660_circ_g.6 AT5G09660_circ_g.7 AT5G09660_circ_g.8 AT5G09660_circ_g.9 AT5G09660_circ_g.10 AT5G09660_circ_g.11 AT5G09660_circ_g.12 AT5G09660_circ_g.13 AT5G09660_circ_g.14 AT5G09690_circ_g.1 AT5G09690_circ_g.2 AT5G09690_circ_g.3 5_circ_ag.4 5_circ_ag.5 5_circ_ag.6 5_circ_ag.7 5_circ_ag.8 5_circ_ag.9 5_circ_ag.10 5_circ_ag.11 ENSRNA049493670_circ_g.1 ENSRNA049493670_circ_g.2 ENSRNA049493670_circ_g.3 ENSRNA049493670_circ_g.4 ENSRNA049493670_circ_g.5 ENSRNA049493670_circ_g.6 						
					
						Support reads
						5
					
						Tissues
						seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G09590.1:2

General Information
CircRNA Name	AT5G09590_circ_g.4
ID in PlantcircBase	ath_circ_037264
Alias	NA
Organism	Arabidpsis thaliana
Position	chr5: 2976315-2977592 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	AT5G09590
Parent gene annotation	Heat shock 70 kDa protein 10, mitochondrial
Parent gene strand	+
Alternative splicing	5_circ_ag.29 AT5G09570_circ_g.1 AT5G09570_circ_g.2 AT5G09570_circ_g.3 AT5G09580_circ_g.1 AT5G09590_circ_g.1 AT5G09590_circ_g.2 AT5G09590_circ_g.3 AT5G09590_circ_g.5 AT5G09590_circ_g.6 AT5G09620_circ_g.1 AT5G09650_circ_g.1 AT5G09650_circ_g.2 AT5G09660_circ_g.1 AT5G09660_circ_g.2 AT5G09660_circ_g.3 AT5G09660_circ_g.4 AT5G09660_circ_g.5 AT5G09660_circ_g.6 AT5G09660_circ_g.7 AT5G09660_circ_g.8 AT5G09660_circ_g.9 AT5G09660_circ_g.10 AT5G09660_circ_g.11 AT5G09660_circ_g.12 AT5G09660_circ_g.13 AT5G09660_circ_g.14 AT5G09690_circ_g.1 AT5G09690_circ_g.2 AT5G09690_circ_g.3 5_circ_ag.4 5_circ_ag.5 5_circ_ag.6 5_circ_ag.7 5_circ_ag.8 5_circ_ag.9 5_circ_ag.10 5_circ_ag.11 ENSRNA049493670_circ_g.1 ENSRNA049493670_circ_g.2 ENSRNA049493670_circ_g.3 ENSRNA049493670_circ_g.4 ENSRNA049493670_circ_g.5 ENSRNA049493670_circ_g.6
Support reads	5
Tissues	seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G09590.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CAACCCACGCATCGCCATTAG
 R: AATCATAAATGAACCGACGGCTGC
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CAACCCACGCATCGCCATTAG R: AATCATAAATGAACCGACGGCTGC PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCTGGATGTCACACCTCTATCGCTCGGTATTGAAACACTTGGTGGTGTCTTTACGAGACTGATC
ACCCGAAATACAACCATCCCCACAAAGAAGAGTCAGaatccgaaagtcattgaaaatgctgaag
gtgctcgaaccactccatcagttgtagcattcaacacgaaaggagaacttcttgtgggtacacc
agccaagc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							AATCCGAAAGTCATTGAAAATGCTGAAGGTGCTCGAACCACTCCATCAGTTGTAGCATTCAACA
CGAAAGGAGAACTTCTTGTGGGTACACCAGCCAAGCGACAAGCTGTCACTAATCCTACAAACAC
AGTATCTGGAACGAAACGTTTGATTGGGAGAAAGTTCGACGATCCACAAACTCAGAAAGAAATG
AAGATGGTGCCTTACAAGATTGTGCGTGCACCTAATGGCGATGCGTGGGTTGAAGCCAATGGTC
AGCAGTATTCTCCTAGTCAGATTGGAGCGTTTATCTTGACGAAGATGAAAGAGACGGCCGAAGC
TTACCTTGGAAAATCTGTCACGAAAGCTGTTGTCACTGTTCCAGCTTACTTTAATGATGCTCAG
AGACAAGCAACAAAAGATGCTGGTAGAATTGCTGGTCTTGATGTTGAGAGAATCATAAATGAAC
CGACGGCTGCTGCTCTGTCCTATGGAATGACCAACAAGGAGGGGTTGATTGCTGTGTTTGATCT
TGGTGGTGGGACATTTGATGTATCTGTTTTGGAGATTTCTAATGGGGTATTCGAGGTAATTATC
TCCCATGTGATCTTATGTTTGTCAGGTCTTCTGTTGCTTTTTCTTTGAGAATCGTTCTTATTAT
TAAATTGCTTTTTTCAGGTGAAAGCCACCAATGGTGATACCTTCTTGGGAGGTGAGGATTTCGA
CAATGCTCTGCTAGACTTCTTGGTTAATGAGTTCAAGACCACTGAGGGAATAGACCTAGCCAAA
GACAGACTTGCTCTGCAGAGGCTTAGAGAAGCCGCTGAGAAAGCGAAGATTGAACTGTCATCTA
CTTCTCAAACTGAAATTAATCTCCCATTTATCACAGCCGATGCATCTGGAGCAAAGCATTTCAA
CATCACCCTAACAAGGTCAAGGTTTGAAACTCTTGTGAATCACTTGATTGAGAGGACCCGCGAT
CCTTGCAAGAACTGTCTCAAGGATGCTGGTATAAGTGCTAAGGAAGTTGATGAGGTTCTTCTAG
TTGGAGGAATGACGCGTGTCCCCAAGGTTCAGTCTATTGTTGCGGAGATCTTTGGAAAGAGTCC
AAGCAAAGGTGTCAATCCTGATGAGGCTGTTGCTATGGGAGCTGCACTTCAAGGTGGTATCCTC
CGCGGTGATGTCAAAGAATTGTTGCTTCTGGATGTCACACCTCTATCGCTCGGTATTGAAACAC
TTGGTGGTGTCTTTACGAGACTGATCACCCGAAATACAACCATCCCCACAAAGAAGAGTCAG

Sequences
Splice junction sequence	TCTGGATGTCACACCTCTATCGCTCGGTATTGAAACACTTGGTGGTGTCTTTACGAGACTGATC ACCCGAAATACAACCATCCCCACAAAGAAGAGTCAGaatccgaaagtcattgaaaatgctgaag gtgctcgaaccactccatcagttgtagcattcaacacgaaaggagaacttcttgtgggtacacc agccaagc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	AATCCGAAAGTCATTGAAAATGCTGAAGGTGCTCGAACCACTCCATCAGTTGTAGCATTCAACA CGAAAGGAGAACTTCTTGTGGGTACACCAGCCAAGCGACAAGCTGTCACTAATCCTACAAACAC AGTATCTGGAACGAAACGTTTGATTGGGAGAAAGTTCGACGATCCACAAACTCAGAAAGAAATG AAGATGGTGCCTTACAAGATTGTGCGTGCACCTAATGGCGATGCGTGGGTTGAAGCCAATGGTC AGCAGTATTCTCCTAGTCAGATTGGAGCGTTTATCTTGACGAAGATGAAAGAGACGGCCGAAGC TTACCTTGGAAAATCTGTCACGAAAGCTGTTGTCACTGTTCCAGCTTACTTTAATGATGCTCAG AGACAAGCAACAAAAGATGCTGGTAGAATTGCTGGTCTTGATGTTGAGAGAATCATAAATGAAC CGACGGCTGCTGCTCTGTCCTATGGAATGACCAACAAGGAGGGGTTGATTGCTGTGTTTGATCT TGGTGGTGGGACATTTGATGTATCTGTTTTGGAGATTTCTAATGGGGTATTCGAGGTAATTATC TCCCATGTGATCTTATGTTTGTCAGGTCTTCTGTTGCTTTTTCTTTGAGAATCGTTCTTATTAT TAAATTGCTTTTTTCAGGTGAAAGCCACCAATGGTGATACCTTCTTGGGAGGTGAGGATTTCGA CAATGCTCTGCTAGACTTCTTGGTTAATGAGTTCAAGACCACTGAGGGAATAGACCTAGCCAAA GACAGACTTGCTCTGCAGAGGCTTAGAGAAGCCGCTGAGAAAGCGAAGATTGAACTGTCATCTA CTTCTCAAACTGAAATTAATCTCCCATTTATCACAGCCGATGCATCTGGAGCAAAGCATTTCAA CATCACCCTAACAAGGTCAAGGTTTGAAACTCTTGTGAATCACTTGATTGAGAGGACCCGCGAT CCTTGCAAGAACTGTCTCAAGGATGCTGGTATAAGTGCTAAGGAAGTTGATGAGGTTCTTCTAG TTGGAGGAATGACGCGTGTCCCCAAGGTTCAGTCTATTGTTGCGGAGATCTTTGGAAAGAGTCC AAGCAAAGGTGTCAATCCTGATGAGGCTGTTGCTATGGGAGCTGCACTTCAAGGTGGTATCCTC CGCGGTGATGTCAAAGAATTGTTGCTTCTGGATGTCACACCTCTATCGCTCGGTATTGAAACAC TTGGTGGTGTCTTTACGAGACTGATCACCCGAAATACAACCATCCCCACAAAGAAGAGTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.374847233

Conservation Information
Conserved circRNAs	NA
PMCS	0.374847233

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							2976504-2977589(+)
						
					

						Potential amino acid sequence
						
							MKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKETAEAYLGKSVTKAVVTVPAYFNDA
QRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIAVFDLGGGTFDVSVLEISNGVFEVK
ATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLALQRLREAAEKAKIELSSTSQTEINL
PFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVP
KVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRL
ITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPTNTVSGTKRLI
GRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKETAEAYLGKSVTK
AVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIAVFDLGGGTFDVS
VLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLALQRLREAAEKAKI
ELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNCLKDAGISAKEVD
EVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVKELLLLDVTPLSL
GIETLGGVFTRLITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTN
PTNTVSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKE
TAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIA
VFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLAL
QRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNC
LKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVK
ELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELL
VGTPAKRQAVTNPTNTVSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPS
QIGAFILTKMKETAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAAL
SYGMTNKEGLIAVFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTT
EGIDLAKDRLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNH
LIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGA
ALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQ(+)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						responsive to heat stress
					

			
		

Functional Information
Coding potential	Y
Potential coding position	2976504-2977589(+)
Potential amino acid sequence	MKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKETAEAYLGKSVTKAVVTVPAYFNDA QRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIAVFDLGGGTFDVSVLEISNGVFEVK ATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLALQRLREAAEKAKIELSSTSQTEINL PFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVP KVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRL ITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPTNTVSGTKRLI GRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKETAEAYLGKSVTK AVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIAVFDLGGGTFDVS VLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLALQRLREAAEKAKI ELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNCLKDAGISAKEVD EVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVKELLLLDVTPLSL GIETLGGVFTRLITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTN PTNTVSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKE TAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKEGLIA VFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLAL QRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNHLIERTRDPCKNC LKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGAALQGGILRGDVK ELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQNPKVIENAEGARTTPSVVAFNTKGELL VGTPAKRQAVTNPTNTVSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPS QIGAFILTKMKETAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAAL SYGMTNKEGLIAVFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTT EGIDLAKDRLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNH LIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAVAMGA ALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT5G09590_circ_g.4

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