Plant Circular RNA Database

Detail information of AT2G32000_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G32000_circ_g.2
					
						ID in PlantcircBase
						ath_circ_016022
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 13617867-13619218  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT2G32000						
					
						Parent gene annotation
						
							DNA topoisomerase 3-beta
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT2G32000_circ_g.3 AT2G32000_circ_g.4 AT2G32000_circ_g.5 AT2G32000_circ_g.6 AT2G32000_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G32000.2:4
AT2G32000.1:4

General Information
CircRNA Name	AT2G32000_circ_g.2
ID in PlantcircBase	ath_circ_016022
Alias	NA
Organism	Arabidpsis thaliana
Position	chr2: 13617867-13619218 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT2G32000
Parent gene annotation	DNA topoisomerase 3-beta
Parent gene strand	-
Alternative splicing	AT2G32000_circ_g.3 AT2G32000_circ_g.4 AT2G32000_circ_g.5 AT2G32000_circ_g.6 AT2G32000_circ_g.7
Support reads	1
Tissues	whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G32000.2:4 AT2G32000.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATAAGGTCGCAGAGCCCAAAACTTTTCTGGCTTGAAAGTATTAATATGCATGTAGCGTTGCACA
CAAAACCCAAGAGTAGGTGTCTGGCATGGCCCATACcttatgtatttgcagttaggtgaaacag
tgccaagaaaatgttgacagacatactgatagagtcgccaagcatctcctccgaccatgacctc
ggttgctg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 642
Transcript exons: 13617867-13618145,13618403-13618482,13618838-13618957,13
619056-13619218
CTTATGTATTTGCAGTTAGGTGAAACAGTGCCAAGAAAATGTTGACAGACATACTGATAGAGTC
GCCAAGCATCTCCTCCGACCATGACCTCGGTTGCTGCTCTCATTGGGGTGATAGGAGGATGGTC
TCCAGCGTCAGTTCCTGACTTTGGCATATGAAATCCGTCTGAGAGCAGTCTCTGTACGTAACCA
CCCCATACTGGATTACTAACTTGTGCTCTGAGTGTGTCTGTGAAGTCGAATGAAGAAGGATAGG
CTGTGCTTTCTGTGCGGGGATAGCTGATGAAACCTTGAGTATACAATCGCTCTGCAAGATGCAT
AGCCGTCTGAGGCCCAAACCCTAGAGCACTTGAAGCAACCTTTAGCAAATTCACTGTGTTAAGA
CCAGCTGGGCGACCTTTGACTTCCTGCTTTTCTGATACGTCCATTACTTTTGCAGTTCTTCCTT
CCACGACCAACTTTTGGAACACAGTAGCAGCTTCCAGGTCGAACAATCTTCGTCTCTCCCACTC
TAGTTGGAGCTCATAACCATCCTTTCTTATATAAGGTCGCAGAGCCCAAAACTTTTCTGGCTTG
AAAGTATTAATATGCATGTAGCGTTGCACACAAAACCCAAGAGTAGGTGTCTGGCATGGCCCAT
AC
						
					

						Genomic sequence
						
							CTTATGTATTTGCAGTTAGGTGAAACAGTGCCAAGAAAATGTTGACAGACATACTGATAGAGTC
GCCAAGCATCTCCTCCGACCATGACCTCGGTTGCTGCTCTCATTGGGGTGATAGGAGGATGGTC
TCCAGCGTCAGTTCCTGACTTTGGCATATGAAATCCGTCTGAGAGCAGTCTCTGTACGTAACCA
CCCCATACTGGATTACTAACTTGTGCTCTGAGTGTGTCTGTGAAGTCGAATGAAGAAGGATAGG
CTGTGCTTTCTGTGCGGGGATAGCTAGCACAAAACAAAGGACTTTGAGTCAAAAGTTTCCGTCA
CTTTAAATGTTTAAATAAATTATACAGCGAAAATTGTGATGTATGTATGTTCTCGTCAGCGACG
GTTTTTTCCATTTCCTATCTACCAGTTCTTAATTAAACAAACAAAACTACCATTCAATAACGAA
TTGGGATTTACCAAAATTAGCCAATTATACGCTGGTAAAGAGGAGTTAAATGACAAAAATAATA
ATCTGGAGAATGGAGCAATCGAACCTGATGAAACCTTGAGTATACAATCGCTCTGCAAGATGCA
TAGCCGTCTGAGGCCCAAACCCTAGAGCACTTGAAGCAACCTGTGTAACCACTAATGTTATGCT
CGATCAGGAGAGTAATTCGCATAAAAGTAGCTTTTATTGACCTTTGAACTCGTGAACAAGACTA
AGCAATGGCAAAGCTCTAATCCCAACATTAAAAACAGAATTAACAAAACAAGAAGAGAACGAAG
ATCCCAATGTTGTTGCCAACTCATCCCCAAAAACAATTATGAAGCTGATATAAACAAAATTTCT
TTGGATGCAGAATATTTCAGTTTTTCAGTCAGTGTACTGCGATCATTAGTACATGATATGGTGT
TTGCTTAAAGTATAATGTACAACCAGGCGAGTTCATTTTAAAGGAATGGTAATCACGACTCCTG
ACTTTTCTGACCTTTAGCAAATTCACTGTGTTAAGACCAGCTGGGCGACCTTTGACTTCCTGCT
TTTCTGATACGTCCATTACTTTTGCAGTTCTTCCTTCCACGACCAACTTTTGGAACACAGTAGC
AGCCTATAATTTGTTAAAGCATATATATATCAGCAACATAAGATTAGAAAGCAACAGTCAAGAA
TGTCAAAGAAATTCTAAGACAAGAATGAGACACTAACTTCCAGGTCGAACAATCTTCGTCTCTC
CCACTCTAGTTGGAGCTCATAACCATCCTTTCTTATATAAGGTCGCAGAGCCCAAAACTTTTCT
GGCTTGAAAGTATTAATATGCATGTAGCGTTGCACACAAAACCCAAGAGTAGGTGTCTGGCATG
GCCCATAC
						
					

			
		

Sequences
Splice junction sequence	ATAAGGTCGCAGAGCCCAAAACTTTTCTGGCTTGAAAGTATTAATATGCATGTAGCGTTGCACA CAAAACCCAAGAGTAGGTGTCTGGCATGGCCCATACcttatgtatttgcagttaggtgaaacag tgccaagaaaatgttgacagacatactgatagagtcgccaagcatctcctccgaccatgacctc ggttgctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 642 Transcript exons: 13617867-13618145,13618403-13618482,13618838-13618957,13 619056-13619218 CTTATGTATTTGCAGTTAGGTGAAACAGTGCCAAGAAAATGTTGACAGACATACTGATAGAGTC GCCAAGCATCTCCTCCGACCATGACCTCGGTTGCTGCTCTCATTGGGGTGATAGGAGGATGGTC TCCAGCGTCAGTTCCTGACTTTGGCATATGAAATCCGTCTGAGAGCAGTCTCTGTACGTAACCA CCCCATACTGGATTACTAACTTGTGCTCTGAGTGTGTCTGTGAAGTCGAATGAAGAAGGATAGG CTGTGCTTTCTGTGCGGGGATAGCTGATGAAACCTTGAGTATACAATCGCTCTGCAAGATGCAT AGCCGTCTGAGGCCCAAACCCTAGAGCACTTGAAGCAACCTTTAGCAAATTCACTGTGTTAAGA CCAGCTGGGCGACCTTTGACTTCCTGCTTTTCTGATACGTCCATTACTTTTGCAGTTCTTCCTT CCACGACCAACTTTTGGAACACAGTAGCAGCTTCCAGGTCGAACAATCTTCGTCTCTCCCACTC TAGTTGGAGCTCATAACCATCCTTTCTTATATAAGGTCGCAGAGCCCAAAACTTTTCTGGCTTG AAAGTATTAATATGCATGTAGCGTTGCACACAAAACCCAAGAGTAGGTGTCTGGCATGGCCCAT AC
Genomic sequence	CTTATGTATTTGCAGTTAGGTGAAACAGTGCCAAGAAAATGTTGACAGACATACTGATAGAGTC GCCAAGCATCTCCTCCGACCATGACCTCGGTTGCTGCTCTCATTGGGGTGATAGGAGGATGGTC TCCAGCGTCAGTTCCTGACTTTGGCATATGAAATCCGTCTGAGAGCAGTCTCTGTACGTAACCA CCCCATACTGGATTACTAACTTGTGCTCTGAGTGTGTCTGTGAAGTCGAATGAAGAAGGATAGG CTGTGCTTTCTGTGCGGGGATAGCTAGCACAAAACAAAGGACTTTGAGTCAAAAGTTTCCGTCA CTTTAAATGTTTAAATAAATTATACAGCGAAAATTGTGATGTATGTATGTTCTCGTCAGCGACG GTTTTTTCCATTTCCTATCTACCAGTTCTTAATTAAACAAACAAAACTACCATTCAATAACGAA TTGGGATTTACCAAAATTAGCCAATTATACGCTGGTAAAGAGGAGTTAAATGACAAAAATAATA ATCTGGAGAATGGAGCAATCGAACCTGATGAAACCTTGAGTATACAATCGCTCTGCAAGATGCA TAGCCGTCTGAGGCCCAAACCCTAGAGCACTTGAAGCAACCTGTGTAACCACTAATGTTATGCT CGATCAGGAGAGTAATTCGCATAAAAGTAGCTTTTATTGACCTTTGAACTCGTGAACAAGACTA AGCAATGGCAAAGCTCTAATCCCAACATTAAAAACAGAATTAACAAAACAAGAAGAGAACGAAG ATCCCAATGTTGTTGCCAACTCATCCCCAAAAACAATTATGAAGCTGATATAAACAAAATTTCT TTGGATGCAGAATATTTCAGTTTTTCAGTCAGTGTACTGCGATCATTAGTACATGATATGGTGT TTGCTTAAAGTATAATGTACAACCAGGCGAGTTCATTTTAAAGGAATGGTAATCACGACTCCTG ACTTTTCTGACCTTTAGCAAATTCACTGTGTTAAGACCAGCTGGGCGACCTTTGACTTCCTGCT TTTCTGATACGTCCATTACTTTTGCAGTTCTTCCTTCCACGACCAACTTTTGGAACACAGTAGC AGCCTATAATTTGTTAAAGCATATATATATCAGCAACATAAGATTAGAAAGCAACAGTCAAGAA TGTCAAAGAAATTCTAAGACAAGAATGAGACACTAACTTCCAGGTCGAACAATCTTCGTCTCTC CCACTCTAGTTGGAGCTCATAACCATCCTTTCTTATATAAGGTCGCAGAGCCCAAAACTTTTCT GGCTTGAAAGTATTAATATGCATGTAGCGTTGCACACAAAACCCAAGAGTAGGTGTCTGGCATG GCCCATAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.216027687

Conservation Information
Conserved circRNAs	NA
PMCS	0.216027687

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13619170-13617869(-)
						
						Potential amino acid sequence
						
							MHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEV
KGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQV
SNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSP
NCKYIRYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATV
FQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISY
PRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVM
VGGDAWRLYQYVCQHFLGTVSPNCKYIRYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRK
DGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSAL
GFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMP
KSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRYGPCQTPTLGFCVQ
RYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQ
EVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRA
QVSNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTV
SPNCKYI(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13619170-13617869(-)
Potential amino acid sequence	MHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEV KGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQV SNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSP NCKYIRYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATV FQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISY PRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVM VGGDAWRLYQYVCQHFLGTVSPNCKYIRYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRK DGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVASSAL GFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLLSDGFHMP KSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRYGPCQTPTLGFCVQ RYMHINTFKPEKFWALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQ EVKGRPAGLNTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRA QVSNPVWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTV SPNCKYI(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT2G32000_circ_g.2

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