Plant Circular RNA Database

Detail information of Zm00001d006496_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d006496_circ_g.2
					
						ID in PlantcircBase
						zma_circ_007370
					
						Alias
						Zm02circ00103, GRMZM2G169615_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr2: 209841387-209841711  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d006496						
					
						Parent gene annotation
						
							Polyadenylate-binding protein RBP45C
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d006496_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d006496_T004:2
Zm00001d006496_T003:2
Zm00001d006496_T002:1
Zm00001d006496_T001:2

General Information
CircRNA Name	Zm00001d006496_circ_g.2
ID in PlantcircBase	zma_circ_007370
Alias	Zm02circ00103, GRMZM2G169615_C1
Organism	Zea mays
Position	chr2: 209841387-209841711 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2
Parent gene	Zm00001d006496
Parent gene annotation	Polyadenylate-binding protein RBP45C
Parent gene strand	+
Alternative splicing	Zm00001d006496_circ_g.1
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d006496_T004:2 Zm00001d006496_T003:2 Zm00001d006496_T002:1 Zm00001d006496_T001:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAGGTCATGTGCTGAGGAAGCCATCCAAATGCTGAATGGGAGTCAGATTGGTGGACAGAAAGCT
CGGCTTTCATGGGGCCGTAGTACTCAAAACAGACAGgtctttgtgggtgggttagattccaatg
tagacgaggagtatctaaggcaaatttttactccgtacggagaaatttcctatgtgaagattcc
tgtaggca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 228
Transcript exons: 209841387-209841517,209841615-209841711
GTCTTTGTGGGTGGGTTAGATTCCAATGTAGACGAGGAGTATCTAAGGCAAATTTTTACTCCGT
ACGGAGAAATTTCCTATGTGAAGATTCCTGTAGGCAAGCATTGTGGATTTGTTCAATTTACCAG
CAGGTCATGTGCTGAGGAAGCCATCCAAATGCTGAATGGGAGTCAGATTGGTGGACAGAAAGCT
CGGCTTTCATGGGGCCGTAGTACTCAAAACAGACAG
						
						Genomic sequence
						
							GTCTTTGTGGGTGGGTTAGATTCCAATGTAGACGAGGAGTATCTAAGGCAAATTTTTACTCCGT
ACGGAGAAATTTCCTATGTGAAGATTCCTGTAGGCAAGCATTGTGGATTTGTTCAATTTACCAG
CAGGTTGACATAAAATGCTTAAAGCTCTTTAATTAACTGTTCTGACACTGATACATGTGAGTTA
ATTCTTTGTTACTTGATTGGGTTACCATGTTTTCAGGTCATGTGCTGAGGAAGCCATCCAAATG
CTGAATGGGAGTCAGATTGGTGGACAGAAAGCTCGGCTTTCATGGGGCCGTAGTACTCAAAACA
GACAG

Sequences
Splice junction sequence	CAGGTCATGTGCTGAGGAAGCCATCCAAATGCTGAATGGGAGTCAGATTGGTGGACAGAAAGCT CGGCTTTCATGGGGCCGTAGTACTCAAAACAGACAGgtctttgtgggtgggttagattccaatg tagacgaggagtatctaaggcaaatttttactccgtacggagaaatttcctatgtgaagattcc tgtaggca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 228 Transcript exons: 209841387-209841517,209841615-209841711 GTCTTTGTGGGTGGGTTAGATTCCAATGTAGACGAGGAGTATCTAAGGCAAATTTTTACTCCGT ACGGAGAAATTTCCTATGTGAAGATTCCTGTAGGCAAGCATTGTGGATTTGTTCAATTTACCAG CAGGTCATGTGCTGAGGAAGCCATCCAAATGCTGAATGGGAGTCAGATTGGTGGACAGAAAGCT CGGCTTTCATGGGGCCGTAGTACTCAAAACAGACAG
Genomic sequence	GTCTTTGTGGGTGGGTTAGATTCCAATGTAGACGAGGAGTATCTAAGGCAAATTTTTACTCCGT ACGGAGAAATTTCCTATGTGAAGATTCCTGTAGGCAAGCATTGTGGATTTGTTCAATTTACCAG CAGGTTGACATAAAATGCTTAAAGCTCTTTAATTAACTGTTCTGACACTGATACATGTGAGTTA ATTCTTTGTTACTTGATTGGGTTACCATGTTTTCAGGTCATGTGCTGAGGAAGCCATCCAAATG CTGAATGGGAGTCAGATTGGTGGACAGAAAGCTCGGCTTTCATGGGGCCGTAGTACTCAAAACA GACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.220199868

Conservation Information
Conserved circRNAs	NA
PMCS	0.220199868

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							209841641-209841708(+)
209841648-209841402(+)
						
						Potential amino acid sequence
						
							MLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLRQIFTPYGEISYVKIPVGKHCGFVQ
FTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLRQIFTPYGEISYV
KIPVGKHCGFVQFTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLR
QIFTPYGEISYVKIPVGKHCGFVQFTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQ(+)
MGVRLVDRKLGFHGAVVLKTDRSLWVG*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	209841641-209841708(+) 209841648-209841402(+)
Potential amino acid sequence	MLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLRQIFTPYGEISYVKIPVGKHCGFVQ FTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLRQIFTPYGEISYV KIPVGKHCGFVQFTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQVFVGGLDSNVDEEYLR QIFTPYGEISYVKIPVGKHCGFVQFTSRSCAEEAIQMLNGSQIGGQKARLSWGRSTQNRQ(+) MGVRLVDRKLGFHGAVVLKTDRSLWVG*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d006496_circ_g.2

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