Plant Circular RNA Database

Detail information of orai.009G350800_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.009G350800_circ_g.2
					
						ID in PlantcircBase
						gra_circ_001019
					
						Alias
						Chr09:44619301|44621508
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr09: 44619301-44621508  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.009G350800						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.009G350800_circ_g.1 orai.009G350800_circ_g.3 orai.009G350800_circ_g.4 orai.009G350800_circ_g.5 orai.009G350800_circ_g.6 orai.009G350800_circ_g.7 						
					
						Support reads
						3
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.009G350800.7:3
Gorai.009G350800.5:3
Gorai.009G350800.6:5
Gorai.009G350800.3:2
Gorai.009G350800.1:5
Gorai.009G350800.4:5
Gorai.009G350800.2:3

General Information
CircRNA Name	orai.009G350800_circ_g.2
ID in PlantcircBase	gra_circ_001019
Alias	Chr09:44619301\|44621508
Organism	Gossypium raimondii
Position	chrChr09: 44619301-44621508 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.009G350800
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.009G350800_circ_g.1 orai.009G350800_circ_g.3 orai.009G350800_circ_g.4 orai.009G350800_circ_g.5 orai.009G350800_circ_g.6 orai.009G350800_circ_g.7
Support reads	3
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.009G350800.7:3 Gorai.009G350800.5:3 Gorai.009G350800.6:5 Gorai.009G350800.3:2 Gorai.009G350800.1:5 Gorai.009G350800.4:5 Gorai.009G350800.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATTGTCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATT
TTGACTATGATGAAGGATACACCTCAGATTGGAAAGgctaacagcagtgctttgctagtctttc
ccggaacagtacattctgaacctcatgaacagatttcatgggctgagtttccatatgttgttga
tgattatg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 544
Transcript exons: 44619301-44619403,44619604-44619668,44620339-44620455,44
620548-44620652,44621355-44621508
GCTAACAGCAGTGCTTTGCTAGTCTTTCCCGGAACAGTACATTCTGAACCTCATGAACAGATTT
CATGGGCTGAGTTTCCATATGTTGTTGATGATTATGGAGATATCTTTTTTGAAATTTTGGATGA
TGAGAACATCTTAAAAGATCGCGGAGCGAGTAACCTTGTGAATGTTCTGATTGGGATGGACATT
CCCGTACGAGAGAATAACCAGGCAGTCGGTGACTTCAACTATTCGGACATCGGGATTGATGATG
AAGTTCCTTTTGACGATGAATATTTTGAGGTTATGGATTCTGAAATATACGAGGCTCCGGTTCG
TTGGGGGATGCCAGATTCGGCTGATAGCAGCTGGGTTCATCCTATATATTTTGCCAAGTGCTTG
ATGAAGGCTGTTCATGTAGAACATGACAGGAAAATGGACCATCCCTCTAATGGTGTTTCGATTG
TCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATTTTGA
CTATGATGAAGGATACACCTCAGATTGGAAAG
						
					

						Genomic sequence
						
							GCTAACAGCAGTGCTTTGCTAGTCTTTCCCGGAACAGTACATTCTGAACCTCATGAACAGATTT
CATGGGCTGAGTTTCCATATGTTGTTGATGATTATGGAGGTAAGTTTATTGGAGACCACCTTTG
AGCTCTATTAGGCTTTTATTCCCGTCAAGGCTTTAGGATTTGTCTCCAGTTTCCTTGTATATGT
ATACAAATGAGCACACACCTACATATGCATATATATATATATATATATTAAACCATTAGTTCTC
TTTATGTGTTAACCTAATTTATGTGTTATCTTTTCTTTCAAATTTAGATATCTTTTTTGAAATT
TTGGATGATGAGAACATCTTAAAAGATCGCGGAGCGAGTAACCTTGTGGTGAGGATATCATTTC
TACTCAAATGTTGTTTTGATTTTCTAGCTCAAGTTTGGTGCCACTCGTTACTTCAAAATTACGT
GTCATTTGGCTAATCATTTAGAAGCTGCTAGTGTAACAGTTTTCATGCATTGCAACGTTGTTTT
GACTCTTCATCTTTTTTTAAGTATATGTGTGTGACACATATTTGGACATGGGTATTGGAATATG
ATTCTCAAAAGATCTTTCAAATACATGGAAAAAACTTTAAAAAATTGAATATACTCGTATCAAA
TACATACTTGCATCCGATGCTCTCATATGAGTATGAGAATCCAACGGAATGGTGATTAGTTAAG
CTTGTTGAGATATCATAGCAAAATCTGTTACTCAGGCTCAAGTGTGAGTGTTTAATAAGGGTGT
GTCCAACATACGTATATTTAAGTTTGTTCCGTGTATCTAGAGGGTTCCTAGAGGGTTATACTCT
TGCCGAAATATATGTTGGACATGGTTATTTTAAGAAAAGTGAAGAGAGCCAGAACACCATAGAC
TACAACAAATGTTCAAAATAGAAATGCTAACAGTTGATTAATGGAATTATTTTTTCTAAGTAAC
TCTACATTTCCACTTGACTTTAATTCCTATTCAATGAGTGCCATATTCTAAAATTTGTAATGAT
TTCTTTTCTTTCAGAATGTTCTGATTGGGATGGACATTCCCGTACGAGAGAATAACCAGGCAGT
CGGTGACTTCAACTATTCGGACATCGGGATTGATGATGAAGTTCCTTTTGACGATGAATATTTT
GAGGTTAGAAACCACTGATTATTGACTTCTGAAGTGAAATCTGTGATATTCTCTTTTGTTAATT
TTGGCTACTCAAGTCCCATTCACGTAAATAGGTTATGGATTCTGAAATATACGAGGCTCCGGTT
CGTTGGGGGATGCCAGATTCGGCTGATAGCAGCTGGGTTCATCCTATATATTTTGCCAAGTGCT
TGATGAAGGTGATTGTGCCTGAGTTTCCCTTAAGTACTAGTTGTATTATAAGTTTTAATTGTAC
TCAATGACTATGTTACTCGGACTCTAGACTGATGGGTTATGCGTCTGACACGGGTATGCTCAAG
TTTTCTTAGTTTTTCTATATATATGGTGGATTATACCCTATACCCATGCTGAGTATGTGTCAAA
CACAAGTCTTGGACACTGATACTTAAAGAAAAATAAAGAGTTCCAAGTAACATGGATCAATGAT
ATCAGGGATGAAGCAAGCCCGGTATGCCCCCCCCCCGGCTAAACAGGATAATTGCAGTTATAGC
CCCTTGTAATAATCAATTAGTTAATTTAAGCTTTTTTGACCCTTTGATTCATGGAAAAATTGCA
TTTTTCCCTCTAAAAAAAGTAGTAATTCTGTTTTTTTTTGGCCAAACCAAAAAAAAAAAAAGTA
ATTCAATTTAAACCCTTTTAACAAAATTTTTTAGCTTTGGCCCTCCTTAGATTTTATAATTTTA
TATCAGTCCCTGAATGATATTACGAGTTTTATCCTTTTCTATCTCCAATATGCTGCTAAGACAG
TTTTTATCATGAGTTTGGTAAGGCAGAAGTCTTAATTGGATCGAAGAGAATTCATGAGATGATG
AGTAGATGCATATTTGCATTTTGAATTTTATAGGTAACTCTGTTTACACTTAGTTTCTGCATTC
ATGCAGGCTGTTCATGTAGAACATGACAGGAAAATGGACCATCCCTCTAATGGTGTTTCGATTG
TCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATTTTGA
CTATGATGAAGGATACACCTCAGATTGGAAAG
						
					

			
		

Sequences
Splice junction sequence	ATTGTCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATT TTGACTATGATGAAGGATACACCTCAGATTGGAAAGgctaacagcagtgctttgctagtctttc ccggaacagtacattctgaacctcatgaacagatttcatgggctgagtttccatatgttgttga tgattatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 544 Transcript exons: 44619301-44619403,44619604-44619668,44620339-44620455,44 620548-44620652,44621355-44621508 GCTAACAGCAGTGCTTTGCTAGTCTTTCCCGGAACAGTACATTCTGAACCTCATGAACAGATTT CATGGGCTGAGTTTCCATATGTTGTTGATGATTATGGAGATATCTTTTTTGAAATTTTGGATGA TGAGAACATCTTAAAAGATCGCGGAGCGAGTAACCTTGTGAATGTTCTGATTGGGATGGACATT CCCGTACGAGAGAATAACCAGGCAGTCGGTGACTTCAACTATTCGGACATCGGGATTGATGATG AAGTTCCTTTTGACGATGAATATTTTGAGGTTATGGATTCTGAAATATACGAGGCTCCGGTTCG TTGGGGGATGCCAGATTCGGCTGATAGCAGCTGGGTTCATCCTATATATTTTGCCAAGTGCTTG ATGAAGGCTGTTCATGTAGAACATGACAGGAAAATGGACCATCCCTCTAATGGTGTTTCGATTG TCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATTTTGA CTATGATGAAGGATACACCTCAGATTGGAAAG
Genomic sequence	GCTAACAGCAGTGCTTTGCTAGTCTTTCCCGGAACAGTACATTCTGAACCTCATGAACAGATTT CATGGGCTGAGTTTCCATATGTTGTTGATGATTATGGAGGTAAGTTTATTGGAGACCACCTTTG AGCTCTATTAGGCTTTTATTCCCGTCAAGGCTTTAGGATTTGTCTCCAGTTTCCTTGTATATGT ATACAAATGAGCACACACCTACATATGCATATATATATATATATATATTAAACCATTAGTTCTC TTTATGTGTTAACCTAATTTATGTGTTATCTTTTCTTTCAAATTTAGATATCTTTTTTGAAATT TTGGATGATGAGAACATCTTAAAAGATCGCGGAGCGAGTAACCTTGTGGTGAGGATATCATTTC TACTCAAATGTTGTTTTGATTTTCTAGCTCAAGTTTGGTGCCACTCGTTACTTCAAAATTACGT GTCATTTGGCTAATCATTTAGAAGCTGCTAGTGTAACAGTTTTCATGCATTGCAACGTTGTTTT GACTCTTCATCTTTTTTTAAGTATATGTGTGTGACACATATTTGGACATGGGTATTGGAATATG ATTCTCAAAAGATCTTTCAAATACATGGAAAAAACTTTAAAAAATTGAATATACTCGTATCAAA TACATACTTGCATCCGATGCTCTCATATGAGTATGAGAATCCAACGGAATGGTGATTAGTTAAG CTTGTTGAGATATCATAGCAAAATCTGTTACTCAGGCTCAAGTGTGAGTGTTTAATAAGGGTGT GTCCAACATACGTATATTTAAGTTTGTTCCGTGTATCTAGAGGGTTCCTAGAGGGTTATACTCT TGCCGAAATATATGTTGGACATGGTTATTTTAAGAAAAGTGAAGAGAGCCAGAACACCATAGAC TACAACAAATGTTCAAAATAGAAATGCTAACAGTTGATTAATGGAATTATTTTTTCTAAGTAAC TCTACATTTCCACTTGACTTTAATTCCTATTCAATGAGTGCCATATTCTAAAATTTGTAATGAT TTCTTTTCTTTCAGAATGTTCTGATTGGGATGGACATTCCCGTACGAGAGAATAACCAGGCAGT CGGTGACTTCAACTATTCGGACATCGGGATTGATGATGAAGTTCCTTTTGACGATGAATATTTT GAGGTTAGAAACCACTGATTATTGACTTCTGAAGTGAAATCTGTGATATTCTCTTTTGTTAATT TTGGCTACTCAAGTCCCATTCACGTAAATAGGTTATGGATTCTGAAATATACGAGGCTCCGGTT CGTTGGGGGATGCCAGATTCGGCTGATAGCAGCTGGGTTCATCCTATATATTTTGCCAAGTGCT TGATGAAGGTGATTGTGCCTGAGTTTCCCTTAAGTACTAGTTGTATTATAAGTTTTAATTGTAC TCAATGACTATGTTACTCGGACTCTAGACTGATGGGTTATGCGTCTGACACGGGTATGCTCAAG TTTTCTTAGTTTTTCTATATATATGGTGGATTATACCCTATACCCATGCTGAGTATGTGTCAAA CACAAGTCTTGGACACTGATACTTAAAGAAAAATAAAGAGTTCCAAGTAACATGGATCAATGAT ATCAGGGATGAAGCAAGCCCGGTATGCCCCCCCCCCGGCTAAACAGGATAATTGCAGTTATAGC CCCTTGTAATAATCAATTAGTTAATTTAAGCTTTTTTGACCCTTTGATTCATGGAAAAATTGCA TTTTTCCCTCTAAAAAAAGTAGTAATTCTGTTTTTTTTTGGCCAAACCAAAAAAAAAAAAAGTA ATTCAATTTAAACCCTTTTAACAAAATTTTTTAGCTTTGGCCCTCCTTAGATTTTATAATTTTA TATCAGTCCCTGAATGATATTACGAGTTTTATCCTTTTCTATCTCCAATATGCTGCTAAGACAG TTTTTATCATGAGTTTGGTAAGGCAGAAGTCTTAATTGGATCGAAGAGAATTCATGAGATGATG AGTAGATGCATATTTGCATTTTGAATTTTATAGGTAACTCTGTTTACACTTAGTTTCTGCATTC ATGCAGGCTGTTCATGTAGAACATGACAGGAAAATGGACCATCCCTCTAATGGTGTTTCGATTG TCGGTTGCCTTCGACCAGCCTTTGCTGATGAAGAATCATATCTAAGAAGGCTATTCCATTTTGA CTATGATGAAGGATACACCTCAGATTGGAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							gar_circ_000784* gar_circ_000783* 						
					
						PMCS
						0.522108552

Conservation Information
Conserved circRNAs	gar_circ_000784* gar_circ_000783*
PMCS	0.522108552

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							44620354-44619302(+)
44621479-44619302(+)
						
						Potential amino acid sequence
						
							MDIPVRENNQAVGDFNYSDIGIDDEVPFDDEYFEVMDSEIYEAPVRWGMPDSADSSWVHPIYFA
KCLMKAVHVEHDRKMDHPSNGVSIVGCLRPAFADEESYLRRLFHFDYDEGYTSDWKG*(+)
MMKDTPQIGKANSSALLVFPGTVHSEPHEQISWAEFPYVVDDYGDIFFEILDDENILKDRGASN
LVNVLIGMDIPVRENNQAVGDFNYSDIGIDDEVPFDDEYFEVMDSEIYEAPVRWGMPDSADSSW
VHPIYFAKCLMKAVHVEHDRKMDHPSNGVSIVGCLRPAFADEESYLRRLFHFDYDEGYTSDWKG
*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	44620354-44619302(+) 44621479-44619302(+)
Potential amino acid sequence	MDIPVRENNQAVGDFNYSDIGIDDEVPFDDEYFEVMDSEIYEAPVRWGMPDSADSSWVHPIYFA KCLMKAVHVEHDRKMDHPSNGVSIVGCLRPAFADEESYLRRLFHFDYDEGYTSDWKG(+) MMKDTPQIGKANSSALLVFPGTVHSEPHEQISWAEFPYVVDDYGDIFFEILDDENILKDRGASN LVNVLIGMDIPVRENNQAVGDFNYSDIGIDDEVPFDDEYFEVMDSEIYEAPVRWGMPDSADSSW VHPIYFAKCLMKAVHVEHDRKMDHPSNGVSIVGCLRPAFADEESYLRRLFHFDYDEGYTSDWKG (+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.009G350800_circ_g.2

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