Plant Circular RNA Database

Detail information of Os08g0143700_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os08g0143700_circ_g.3
					
						ID in PlantcircBase
						osa_circ_035826
					
						Alias
						Os_ciR11514
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 2415587-2416963  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os08g0143700						
					
						Parent gene annotation
						
							Protein of unknown function DUF676, hydrolase-like domain contai
ning protein. (Os08t0143700-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os08g0143700_circ_g.1 Os08g0143700_circ_g.2 Os08g0143700_circ_g.4 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0143700-01:4

General Information
CircRNA Name	Os08g0143700_circ_g.3
ID in PlantcircBase	osa_circ_035826
Alias	Os_ciR11514
Organism	Oryza sativa
Position	chr8: 2415587-2416963 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os08g0143700
Parent gene annotation	Protein of unknown function DUF676, hydrolase-like domain contai ning protein. (Os08t0143700-01)
Parent gene strand	-
Alternative splicing	Os08g0143700_circ_g.1 Os08g0143700_circ_g.2 Os08g0143700_circ_g.4
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0143700-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGTAATGGAAAGAATAAAATGTAGCAACTTACAGTGCACGATTACATCATCAGGAAGTTGCTTT
ACAAATTCGTTAGCCGCATATTGCCAGTCAGCATTAatgaaataaagatcaccataatcttcag
ccatgcgctgcaataatggtggttctccctcatcatcatcagtgagaaaaagatgtttcccagt
tctcctaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 519
Transcript exons: 2415587-2415750,2416371-2416586,2416749-2416819,2416896-
2416963
ATGAAATAAAGATCACCATAATCTTCAGCCATGCGCTGCAATAATGGTGGTTCTCCCTCATCAT
CATCAGTGAGAAAAAGATGTTTCCCAGTTCTCCTAAATATCCAATGGACCACACGAGAAGCAAA
ATTTTCCATTGCAATGGACCCAAAGAGAAGGGGAACCTGATTGTTCCCTCGAGACCCAAGGTGA
GGTGTTGCTACGGTTATAAAGTTCATTGCTTCCAACCCGCCGATGGTTCCTTTCGTTTCACTCT
TGGATGTGTCACTTGGATGTCTATAAAGCTTTGCAATTGCATATCTTGCTGCTAATCCTCCAAC
AGAGTGTGCAAGAAAGGAAATCTTAGTGAGTTCTGGCTTTCGAATGATTACATCGATCACCTCA
TCTGCTAAACGTTCCCCCATAACATCTACACCTTCTAGAGTCAAGGTATTAATGTTTTTCTCAC
TGCAGTGCACGATTACATCATCAGGAAGTTGCTTTACAAATTCGTTAGCCGCATATTGCCAGTC
AGCATTA
						
					

						Genomic sequence
						
							ATGAAATAAAGATCACCATAATCTTCAGCCATGCGCTGCAATAATGGTGGTTCTCCCTCATCAT
CATCAGTGAGAAAAAGATGTTTCCCAGTTCTCCTAAATATCCAATGGACCACACGAGAAGCAAA
ATTTTCCATTGCAATGGACCCAAAGAGAAGGGGAACCTAATTCATAACAGTAAGGACACAGAAC
AGTTATAGGGGTGGATGCAACGCCAAAATGCTCGTCGAATATGGCATATAGAATGCAGAAGGGA
AAAAGGACTTTGAATATCCAGAAAAATCTGAAAACTTTGCTACAAATATAAGCATGCAGCTAAG
TAAAATGGCTCAACTGCACAAGTTACAAAAAACGTATCTGAAGTCTTTCGTACATGAGTTCTCA
GAACTTGGAGGAGCAACGGACAAGAAGCACAAGCCCTTTTTGTGTGTTTATTATAAGAAAACAA
TGGACATCTAGTACTTAAAAAATTATATATGGGGCATCGAATGCTCTGTTGCCAGAAAAAAACA
CTGAGCAATGGCATATCTGGCACTTAAAAAAACTATGACCCCAATAGACTGTAAATCCATGATG
CGTGAAAGAAGTATAATACCTGATACAAACAGAAAAATGGATAGATAAAATAAATGAGAAAATT
TTGGGGCATTGATTTGAGGACATTTTATCATACATAGACATGATATATAACACCTTCCATCCCC
CTGATGGTACGTAAAAATTCAGAACTCAAATGGCTGTCGAAGTGAAAAGGGAATACATAAAAAC
AAGTCATCAACAATACCTGATTGTTCCCTCGAGACCCAAGGTGAGGTGTTGCTACGGTTATAAA
GTTCATTGCTTCCAACCCGCCGATGGTTCCTTTCGTTTCACTCTTGGATGTGTCACTTGGATGT
CTATAAAGCTTTGCAATTGCATATCTTGCTGCTAATCCTCCAACAGAGTGTGCAAGAAAGGAAA
TCTTAGTGAGTTCTGGCTTTCGAATGATTACATCGATCACCTGAATAATGGGAGAATAGAAGTG
AGACTCCAATACATATGCTTTGTGATGAGCACAGAGTATTATTCACAACATCATTTCAATGAAA
AATCGCTGACAGAGCAGCTAACCTCCAGATAATAGCCTCTCAAAGTTCAAAACATATGCTGACG
TGGTACTTACCTCATCTGCTAAACGTTCCCCCATAACATCTACACCTTCTAGAGTCAAGGTATT
AATGTTTTTCTCACTGCCTGTAAATTGAAAAAAAAAGTCACTGAAACTAGCAACTGTAGTCAGT
AATGGAAAGAATAAAATGTAGCAACTTACAGTGCACGATTACATCATCAGGAAGTTGCTTTACA
AATTCGTTAGCCGCATATTGCCAGTCAGCATTA
						
					

			
		

Sequences
Splice junction sequence	AGTAATGGAAAGAATAAAATGTAGCAACTTACAGTGCACGATTACATCATCAGGAAGTTGCTTT ACAAATTCGTTAGCCGCATATTGCCAGTCAGCATTAatgaaataaagatcaccataatcttcag ccatgcgctgcaataatggtggttctccctcatcatcatcagtgagaaaaagatgtttcccagt tctcctaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 519 Transcript exons: 2415587-2415750,2416371-2416586,2416749-2416819,2416896- 2416963 ATGAAATAAAGATCACCATAATCTTCAGCCATGCGCTGCAATAATGGTGGTTCTCCCTCATCAT CATCAGTGAGAAAAAGATGTTTCCCAGTTCTCCTAAATATCCAATGGACCACACGAGAAGCAAA ATTTTCCATTGCAATGGACCCAAAGAGAAGGGGAACCTGATTGTTCCCTCGAGACCCAAGGTGA GGTGTTGCTACGGTTATAAAGTTCATTGCTTCCAACCCGCCGATGGTTCCTTTCGTTTCACTCT TGGATGTGTCACTTGGATGTCTATAAAGCTTTGCAATTGCATATCTTGCTGCTAATCCTCCAAC AGAGTGTGCAAGAAAGGAAATCTTAGTGAGTTCTGGCTTTCGAATGATTACATCGATCACCTCA TCTGCTAAACGTTCCCCCATAACATCTACACCTTCTAGAGTCAAGGTATTAATGTTTTTCTCAC TGCAGTGCACGATTACATCATCAGGAAGTTGCTTTACAAATTCGTTAGCCGCATATTGCCAGTC AGCATTA
Genomic sequence	ATGAAATAAAGATCACCATAATCTTCAGCCATGCGCTGCAATAATGGTGGTTCTCCCTCATCAT CATCAGTGAGAAAAAGATGTTTCCCAGTTCTCCTAAATATCCAATGGACCACACGAGAAGCAAA ATTTTCCATTGCAATGGACCCAAAGAGAAGGGGAACCTAATTCATAACAGTAAGGACACAGAAC AGTTATAGGGGTGGATGCAACGCCAAAATGCTCGTCGAATATGGCATATAGAATGCAGAAGGGA AAAAGGACTTTGAATATCCAGAAAAATCTGAAAACTTTGCTACAAATATAAGCATGCAGCTAAG TAAAATGGCTCAACTGCACAAGTTACAAAAAACGTATCTGAAGTCTTTCGTACATGAGTTCTCA GAACTTGGAGGAGCAACGGACAAGAAGCACAAGCCCTTTTTGTGTGTTTATTATAAGAAAACAA TGGACATCTAGTACTTAAAAAATTATATATGGGGCATCGAATGCTCTGTTGCCAGAAAAAAACA CTGAGCAATGGCATATCTGGCACTTAAAAAAACTATGACCCCAATAGACTGTAAATCCATGATG CGTGAAAGAAGTATAATACCTGATACAAACAGAAAAATGGATAGATAAAATAAATGAGAAAATT TTGGGGCATTGATTTGAGGACATTTTATCATACATAGACATGATATATAACACCTTCCATCCCC CTGATGGTACGTAAAAATTCAGAACTCAAATGGCTGTCGAAGTGAAAAGGGAATACATAAAAAC AAGTCATCAACAATACCTGATTGTTCCCTCGAGACCCAAGGTGAGGTGTTGCTACGGTTATAAA GTTCATTGCTTCCAACCCGCCGATGGTTCCTTTCGTTTCACTCTTGGATGTGTCACTTGGATGT CTATAAAGCTTTGCAATTGCATATCTTGCTGCTAATCCTCCAACAGAGTGTGCAAGAAAGGAAA TCTTAGTGAGTTCTGGCTTTCGAATGATTACATCGATCACCTGAATAATGGGAGAATAGAAGTG AGACTCCAATACATATGCTTTGTGATGAGCACAGAGTATTATTCACAACATCATTTCAATGAAA AATCGCTGACAGAGCAGCTAACCTCCAGATAATAGCCTCTCAAAGTTCAAAACATATGCTGACG TGGTACTTACCTCATCTGCTAAACGTTCCCCCATAACATCTACACCTTCTAGAGTCAAGGTATT AATGTTTTTCTCACTGCCTGTAAATTGAAAAAAAAAGTCACTGAAACTAGCAACTGTAGTCAGT AATGGAAAGAATAAAATGTAGCAACTTACAGTGCACGATTACATCATCAGGAAGTTGCTTTACA AATTCGTTAGCCGCATATTGCCAGTCAGCATTA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.23446752

Conservation Information
Conserved circRNAs	NA
PMCS	0.23446752

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2416571-2415592(+)
2416774-2415589(-)
2415655-2416928(-)
						
						Potential amino acid sequence
						
							MITSITSSAKRSPITSTPSRVKVLMFFSLQCTITSSGSCFTNSLAAYCQSALMK*(+)
MGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTIGGLEAMN
FITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPLLQRMAED
YGDLYFINADWQYAANEFVKQLPDDVIVHCSEKNINTLTLEGVDVMGERLADEVIDVIIRKPEL
TKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTIGGLEAMNFITVATPHLGSRGNNQVPL
LFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPLLQRMAEDYGDLYFINADWQYAANEFV
KQLPDDVIVHCSEKNINTLTLEGVDVMGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAI
AKLYRHPSDTSKSETKGTIGGLEAMNFITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIF
RRTGKHLFLTDDDEGEPPLLQRMAEDYGDLYFINADWQYAANEFVKQLPDDVIVHCSEKNINTL
TLEGVDVMGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTI
GGLEAMNFITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPL
LQRMAEDYGDLYF(-)
MMMRENHHYCSAWLKIMVIFISLMLTGNMRLTNL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2416571-2415592(+) 2416774-2415589(-) 2415655-2416928(-)
Potential amino acid sequence	MITSITSSAKRSPITSTPSRVKVLMFFSLQCTITSSGSCFTNSLAAYCQSALMK(+) MGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTIGGLEAMN FITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPLLQRMAED YGDLYFINADWQYAANEFVKQLPDDVIVHCSEKNINTLTLEGVDVMGERLADEVIDVIIRKPEL TKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTIGGLEAMNFITVATPHLGSRGNNQVPL LFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPLLQRMAEDYGDLYFINADWQYAANEFV KQLPDDVIVHCSEKNINTLTLEGVDVMGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAI AKLYRHPSDTSKSETKGTIGGLEAMNFITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIF RRTGKHLFLTDDDEGEPPLLQRMAEDYGDLYFINADWQYAANEFVKQLPDDVIVHCSEKNINTL TLEGVDVMGERLADEVIDVIIRKPELTKISFLAHSVGGLAARYAIAKLYRHPSDTSKSETKGTI GGLEAMNFITVATPHLGSRGNNQVPLLFGSIAMENFASRVVHWIFRRTGKHLFLTDDDEGEPPL LQRMAEDYGDLYF(-) MMMRENHHYCSAWLKIMVIFISLMLTGNMRLTNL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os08g0143700_circ_g.3

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