Plant Circular RNA Database

Detail information of Zm00001d024587_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d024587_circ_g.2
					
						ID in PlantcircBase
						zma_circ_010231
					
						Alias
						zma_circ_0000179, GRMZM2G086496_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr10: 79467391-79467995  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI2
					
						Parent gene
						
							Zm00001d024587						
					
						Parent gene annotation
						
							Protein NRT1/ PTR FAMILY 6.3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d024587_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d024587_T001:1

General Information
CircRNA Name	Zm00001d024587_circ_g.2
ID in PlantcircBase	zma_circ_010231
Alias	zma_circ_0000179, GRMZM2G086496_C1
Organism	Zea mays
Position	chr10: 79467391-79467995 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	find_circ, CIRI2
Parent gene	Zm00001d024587
Parent gene annotation	Protein NRT1/ PTR FAMILY 6.3
Parent gene strand	+
Alternative splicing	Zm00001d024587_circ_g.1
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d024587_T001:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCTACGACGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAA
GCGCAAGGAGCGCCTCCCCCACACCGACCAGTTCCGggcgtgacgatcctgacgatctcgacgg
cggcgccggggctacggccggcgtcctgctccgcgaccggcggaggcgtcgtcggggagtgcgc
gcgggcgt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 605
Transcript exons: 79467391-79467995
GGCGTGACGATCCTGACGATCTCGACGGCGGCGCCGGGGCTACGGCCGGCGTCCTGCTCCGCGA
CCGGCGGAGGCGTCGTCGGGGAGTGCGCGCGGGCGTCGGGGGCGCAGCTGGGGGTGCTGTACCT
GGCGCTGTACCTGACGGCGCTGGGCACGGGTGGGCTAAAGTCGAGCGTGTCGGGGTTCGGGTCG
GACCAGTTCGACGAGTCGGACGGCGGGGAGAAGCGGCAGATGATGCGCTTCTTCAACTGGTTCT
TCTTCTTCATCTCGCTGGGGTCGCTGCTGGCCGTCACCGTGCTGGTGTACGTCCAGGACAACCT
GGGCAGGCGCTGGGGCTACGGCGCCTGCGCCTGCGCCATCGCCGCGGGCCTCCTCGTCTTCCTG
GCCGGCACACGCAGGTACCGCTTCAAGAAGCTGGCCGGCAGCCCCCTCACGCAGATCGCCGCCG
TCGTCGTCGCCGCCTGGCGCAAGCGCCGCCTCCCTCTCCCCGCCGACCCCGCCATGCTCTACGA
CGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAAGCGCAAG
GAGCGCCTCCCCCACACCGACCAGTTCCG
						
						Genomic sequence
						
							GGCGTGACGATCCTGACGATCTCGACGGCGGCGCCGGGGCTACGGCCGGCGTCCTGCTCCGCGA
CCGGCGGAGGCGTCGTCGGGGAGTGCGCGCGGGCGTCGGGGGCGCAGCTGGGGGTGCTGTACCT
GGCGCTGTACCTGACGGCGCTGGGCACGGGTGGGCTAAAGTCGAGCGTGTCGGGGTTCGGGTCG
GACCAGTTCGACGAGTCGGACGGCGGGGAGAAGCGGCAGATGATGCGCTTCTTCAACTGGTTCT
TCTTCTTCATCTCGCTGGGGTCGCTGCTGGCCGTCACCGTGCTGGTGTACGTCCAGGACAACCT
GGGCAGGCGCTGGGGCTACGGCGCCTGCGCCTGCGCCATCGCCGCGGGCCTCCTCGTCTTCCTG
GCCGGCACACGCAGGTACCGCTTCAAGAAGCTGGCCGGCAGCCCCCTCACGCAGATCGCCGCCG
TCGTCGTCGCCGCCTGGCGCAAGCGCCGCCTCCCTCTCCCCGCCGACCCCGCCATGCTCTACGA
CGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAAGCGCAAG
GAGCGCCTCCCCCACACCGACCAGTTCCG

Sequences
Splice junction sequence	TCTACGACGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAA GCGCAAGGAGCGCCTCCCCCACACCGACCAGTTCCGggcgtgacgatcctgacgatctcgacgg cggcgccggggctacggccggcgtcctgctccgcgaccggcggaggcgtcgtcggggagtgcgc gcgggcgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 605 Transcript exons: 79467391-79467995 GGCGTGACGATCCTGACGATCTCGACGGCGGCGCCGGGGCTACGGCCGGCGTCCTGCTCCGCGA CCGGCGGAGGCGTCGTCGGGGAGTGCGCGCGGGCGTCGGGGGCGCAGCTGGGGGTGCTGTACCT GGCGCTGTACCTGACGGCGCTGGGCACGGGTGGGCTAAAGTCGAGCGTGTCGGGGTTCGGGTCG GACCAGTTCGACGAGTCGGACGGCGGGGAGAAGCGGCAGATGATGCGCTTCTTCAACTGGTTCT TCTTCTTCATCTCGCTGGGGTCGCTGCTGGCCGTCACCGTGCTGGTGTACGTCCAGGACAACCT GGGCAGGCGCTGGGGCTACGGCGCCTGCGCCTGCGCCATCGCCGCGGGCCTCCTCGTCTTCCTG GCCGGCACACGCAGGTACCGCTTCAAGAAGCTGGCCGGCAGCCCCCTCACGCAGATCGCCGCCG TCGTCGTCGCCGCCTGGCGCAAGCGCCGCCTCCCTCTCCCCGCCGACCCCGCCATGCTCTACGA CGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAAGCGCAAG GAGCGCCTCCCCCACACCGACCAGTTCCG
Genomic sequence	GGCGTGACGATCCTGACGATCTCGACGGCGGCGCCGGGGCTACGGCCGGCGTCCTGCTCCGCGA CCGGCGGAGGCGTCGTCGGGGAGTGCGCGCGGGCGTCGGGGGCGCAGCTGGGGGTGCTGTACCT GGCGCTGTACCTGACGGCGCTGGGCACGGGTGGGCTAAAGTCGAGCGTGTCGGGGTTCGGGTCG GACCAGTTCGACGAGTCGGACGGCGGGGAGAAGCGGCAGATGATGCGCTTCTTCAACTGGTTCT TCTTCTTCATCTCGCTGGGGTCGCTGCTGGCCGTCACCGTGCTGGTGTACGTCCAGGACAACCT GGGCAGGCGCTGGGGCTACGGCGCCTGCGCCTGCGCCATCGCCGCGGGCCTCCTCGTCTTCCTG GCCGGCACACGCAGGTACCGCTTCAAGAAGCTGGCCGGCAGCCCCCTCACGCAGATCGCCGCCG TCGTCGTCGCCGCCTGGCGCAAGCGCCGCCTCCCTCTCCCCGCCGACCCCGCCATGCTCTACGA CGTCGACGTCGGCAAGGCCGCCGCCGTCGAGGATGGGTCCTCCAGCAAGAAGAGCAAGCGCAAG GAGCGCCTCCCCCACACCGACCAGTTCCG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osa_circ_035900 osi_circ_007539 osi_circ_018203 						
					
						PMCS
						0.363408116

Conservation Information
Conserved circRNAs	osa_circ_035900 osi_circ_007539 osi_circ_018203
PMCS	0.363408116

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							79467623-79467394(+)
79467936-79467394(+)
79467658-79467823(-)
						
						Potential amino acid sequence
						
							MMRFFNWFFFFISLGSLLAVTVLVYVQDNLGRRWGYGACACAIAAGLLVFLAGTRRYRFKKLAG
SPLTQIAAVVVAAWRKRRLPLPADPAMLYDVDVGKAAAVEDGSSSKKSKRKERLPHTDQFRA*(
+)
MGPPARRASARSASPTPTSSGRDDPDDLDGGAGATAGVLLRDRRRRRRGVRAGVGGAAGGAVPG
AVPDGAGHGWAKVERVGVRVGPVRRVGRRGEAADDALLQLVLLLHLAGVAAGRHRAGVRPGQPG
QALGLRRLRLRHRRGPPRLPGRHTQVPLQEAGRQPPHADRRRRRRRLAQAPPPSPRRPRHALRR
RRRQGRRRRGWVLQQEEQAQGAPPPHRPVPGVTILTISTAAPGLRPASCSATGGGVVGECARAS
GAQLGVLYLALYLTALGTGGLKSSVSGFGSDQFDESDGGEKRQMMRFFNWFFFFISLGSLLAVT
VLVYVQDNLGRRWGYGACACAIAAGLLVFLAGTRRYRFKKLAGSPLTQIAAVVVAAWRKRRLPL
PADPAMLYDVDVGKAAAVEDGSSSKKSKRKERLPHTDQFRA*(+)
MKKKNQLKKRIICRFSPPSDSSNWSDPNPDTLDFSPPVPSAVRYSARYSTPSCAPDARAHSPTT
PPPVAEQDAGRSPGAAVEIVRIVTPGTGRCGGGAPCACSSCWRTHPRRRRPCRRRRRRAWRGRR
GEGGGACARRRRRRRRSA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	79467623-79467394(+) 79467936-79467394(+) 79467658-79467823(-)
Potential amino acid sequence	MMRFFNWFFFFISLGSLLAVTVLVYVQDNLGRRWGYGACACAIAAGLLVFLAGTRRYRFKKLAG SPLTQIAAVVVAAWRKRRLPLPADPAMLYDVDVGKAAAVEDGSSSKKSKRKERLPHTDQFRA( +) MGPPARRASARSASPTPTSSGRDDPDDLDGGAGATAGVLLRDRRRRRRGVRAGVGGAAGGAVPG AVPDGAGHGWAKVERVGVRVGPVRRVGRRGEAADDALLQLVLLLHLAGVAAGRHRAGVRPGQPG QALGLRRLRLRHRRGPPRLPGRHTQVPLQEAGRQPPHADRRRRRRRLAQAPPPSPRRPRHALRR RRRQGRRRRGWVLQQEEQAQGAPPPHRPVPGVTILTISTAAPGLRPASCSATGGGVVGECARAS GAQLGVLYLALYLTALGTGGLKSSVSGFGSDQFDESDGGEKRQMMRFFNWFFFFISLGSLLAVT VLVYVQDNLGRRWGYGACACAIAAGLLVFLAGTRRYRFKKLAGSPLTQIAAVVVAAWRKRRLPL PADPAMLYDVDVGKAAAVEDGSSSKKSKRKERLPHTDQFRA(+) MKKKNQLKKRIICRFSPPSDSSNWSDPNPDTLDFSPPVPSAVRYSARYSTPSCAPDARAHSPTT PPPVAEQDAGRSPGAAVEIVRIVTPGTGRCGGGAPCACSSCWRTHPRRRRPCRRRRRRAWRGRR GEGGGACARRRRRRRRSA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d024587_circ_g.2

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