Plant Circular RNA Database

Detail information of Os05g0302700_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0302700_circ_g.2
					
						ID in PlantcircBase
						osa_circ_027324
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 13611425-13612356  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os05g0302700						
					
						Parent gene annotation
						
							Similar to ATP/ADP carrier protein. (Os05t0302700-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0302750_circ_g.1 Os05g0302700_circ_g.3 						
					
						Support reads
						14
					
						Tissues
						anther
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0302700-01:2

General Information
CircRNA Name	Os05g0302700_circ_g.2
ID in PlantcircBase	osa_circ_027324
Alias	NA
Organism	Oryza sativa
Position	chr5: 13611425-13612356 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os05g0302700
Parent gene annotation	Similar to ATP/ADP carrier protein. (Os05t0302700-01)
Parent gene strand	-
Alternative splicing	Os05g0302750_circ_g.1 Os05g0302700_circ_g.3
Support reads	14
Tissues	anther
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0302700-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAAGAATAAGGTGAGATCTTGCTGAACATCATGGACTGCCCATGTATCTTCTGGAGCACAGTGG
GAGGGCCCAAATCGTCAGCCATCTTCAGAAAACAACctgaaggttgccaacaagaacgactggc
ttgagagaatcatacattccaaagtacagcccacgatagacaatgataccaacacaggagatgt
tgaatcca

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CTGAAGGTTGCCAACAAGAACGACTGGCTTGAGAGAATCATACATTCCAAAGTACAGCCCACGA
TAGACAATGATACCAACACAGGAGATGTTGAATCCACGGTACAGACCAGCAATACCATCAGACG
CAAGGGTCTTGCGGTAAACATCAACAAGTCCATTGAATTGCCTCCCACCACCCTTCTTTGCAGC
CTTGGCATCATTAGCCAGACGAGTACGAGCATAATCCAGAGAATACACAAAGAAGAGAGAGCAA
GCACCAGCAGCTCCTCCAGAAGCCAGGTTTCCAGCAAACCACTTCCAGTAACCATCCTTGTCCT
TCTTAAAGTTGAACATGCGCTTGAAATGGTCTTTGAAGGCAAAGTTCAAGGCCTAAGCAAATGT
ACAATATAAGTCAACATCCAAGGGGAGAATAAATAAAAAACAGACAGTAGGATGCAGTACCTGG
GTAGGAAAGTAACGGATGACATTAGCAGTGTTCCCTCTCCACAGTGCAATCACACCTTCATCCT
TAATTGTGCGGCCAAAGCAGTCCGCAATACCTTTGTATGGGTGAGAGAGCCTACCACTCTTGAT
CATTTCATCCTGGTTTTGAATGAGAAGCTTGATTCGCTCAATGGGAGCAGCAGCAGTCTTGGAG
ACAGCAGCTGAAACTCCACCCATCATGAAGTCAATCATAAACCCAGAAAGACCTTTCTCCTTTG
GGGCAGAAGCAAAGACTGGGGATACAGATGTGACAGATGAGAACCCATTGTTCCCATCCATCCC
ATTGTATGACTTCAGAGGCACACTGTAAGAAGTGTTGGCATTGTAGAGTGCGGGATTCTTCATC
AAAGAATAAGGTGAGATCTTGCTGAACATCATGGACTGCCCATGTATCTTCTGGAGCACAGTGG
GAGGGCCCAAATCGTCAGCCATCTTCAGAAAACAAC

Sequences
Splice junction sequence	AAAGAATAAGGTGAGATCTTGCTGAACATCATGGACTGCCCATGTATCTTCTGGAGCACAGTGG GAGGGCCCAAATCGTCAGCCATCTTCAGAAAACAACctgaaggttgccaacaagaacgactggc ttgagagaatcatacattccaaagtacagcccacgatagacaatgataccaacacaggagatgt tgaatcca
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGAAGGTTGCCAACAAGAACGACTGGCTTGAGAGAATCATACATTCCAAAGTACAGCCCACGA TAGACAATGATACCAACACAGGAGATGTTGAATCCACGGTACAGACCAGCAATACCATCAGACG CAAGGGTCTTGCGGTAAACATCAACAAGTCCATTGAATTGCCTCCCACCACCCTTCTTTGCAGC CTTGGCATCATTAGCCAGACGAGTACGAGCATAATCCAGAGAATACACAAAGAAGAGAGAGCAA GCACCAGCAGCTCCTCCAGAAGCCAGGTTTCCAGCAAACCACTTCCAGTAACCATCCTTGTCCT TCTTAAAGTTGAACATGCGCTTGAAATGGTCTTTGAAGGCAAAGTTCAAGGCCTAAGCAAATGT ACAATATAAGTCAACATCCAAGGGGAGAATAAATAAAAAACAGACAGTAGGATGCAGTACCTGG GTAGGAAAGTAACGGATGACATTAGCAGTGTTCCCTCTCCACAGTGCAATCACACCTTCATCCT TAATTGTGCGGCCAAAGCAGTCCGCAATACCTTTGTATGGGTGAGAGAGCCTACCACTCTTGAT CATTTCATCCTGGTTTTGAATGAGAAGCTTGATTCGCTCAATGGGAGCAGCAGCAGTCTTGGAG ACAGCAGCTGAAACTCCACCCATCATGAAGTCAATCATAAACCCAGAAAGACCTTTCTCCTTTG GGGCAGAAGCAAAGACTGGGGATACAGATGTGACAGATGAGAACCCATTGTTCCCATCCATCCC ATTGTATGACTTCAGAGGCACACTGTAAGAAGTGTTGGCATTGTAGAGTGCGGGATTCTTCATC AAAGAATAAGGTGAGATCTTGCTGAACATCATGGACTGCCCATGTATCTTCTGGAGCACAGTGG GAGGGCCCAAATCGTCAGCCATCTTCAGAAAACAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_015285 						
					
						PMCS
						0.481502289

Conservation Information
Conserved circRNAs	osi_circ_015285
PMCS	0.481502289

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13612299-13611425(+)
13612288-13611453(+)
13611737-13612337(-)
13612344-13612347(-)
						
						Potential amino acid sequence
						
							MYLLEHSGRAQIVSHLQKTT*(+)
MDCPCIFWSTVGGPKSSAIFRKQPEGCQQERLA*(+)
MVTGSGLLETWLLEELLVLALSSLCILWIMLVLVWLMMPRLQRRVVGGNSMDLLMFTARPLRLM
VLLVCTVDSTSPVLVSLSIVGCTLECMILSSQSFLLATFRLFSEDG*(-)
MADDLGPPTVLQKIHGQSMMFSKISPYSLMKNPALYNANTSYSVPLKSYNGMDGNNGFSSVTSV
SPVFASAPKEKGLSGFMIDFMMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSHPYKGIA
DCFGRTIKDEGVIALWRGNTANVIRYFPTQALNFAFKDHFKRMFNFKKDKDGYWKWFAGNLASG
GAAGACSLFFVYSLDYARTRLANDAKAAKKGGGRQFNGLVDVYRKTLASDGIAGLYRGFNISCV
GIIVYRGLYFGMYDSLKPVVLVGNLQVVF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13612299-13611425(+) 13612288-13611453(+) 13611737-13612337(-) 13612344-13612347(-)
Potential amino acid sequence	MYLLEHSGRAQIVSHLQKTT(+) MDCPCIFWSTVGGPKSSAIFRKQPEGCQQERLA(+) MVTGSGLLETWLLEELLVLALSSLCILWIMLVLVWLMMPRLQRRVVGGNSMDLLMFTARPLRLM VLLVCTVDSTSPVLVSLSIVGCTLECMILSSQSFLLATFRLFSEDG(-) MADDLGPPTVLQKIHGQSMMFSKISPYSLMKNPALYNANTSYSVPLKSYNGMDGNNGFSSVTSV SPVFASAPKEKGLSGFMIDFMMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSHPYKGIA DCFGRTIKDEGVIALWRGNTANVIRYFPTQALNFAFKDHFKRMFNFKKDKDGYWKWFAGNLASG GAAGACSLFFVYSLDYARTRLANDAKAAKKGGGRQFNGLVDVYRKTLASDGIAGLYRGFNISCV GIIVYRGLYFGMYDSLKPVVLVGNLQVVF(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0302700_circ_g.2

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