Plant Circular RNA Database

Detail information of Os09g0323100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os09g0323100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_038851
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr9: 9447475-9448288  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os09g0323100						
					
						Parent gene annotation
						
							RING finger ubiquitin E3 ligase, Heat tolerance, Modulation of h
ydrogen peroxide-induced stomatal closure (Os09t0323100-01);Zinc
 finger, RING/FYVE/PHD-type domain containing protein. (Os09t032
3100-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os09t0323100-01:1
Os09t0323100-02:1

General Information
CircRNA Name	Os09g0323100_circ_g.2
ID in PlantcircBase	osa_circ_038851
Alias	NA
Organism	Oryza sativa
Position	chr9: 9447475-9448288 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os09g0323100
Parent gene annotation	RING finger ubiquitin E3 ligase, Heat tolerance, Modulation of h ydrogen peroxide-induced stomatal closure (Os09t0323100-01);Zinc finger, RING/FYVE/PHD-type domain containing protein. (Os09t032 3100-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os09t0323100-01:1 Os09t0323100-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTATAAACTTCAT
TTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAGcttggacacgaaattgtgatacatccaa
aatgcaaaacagtaccactgctacgctgattgcgtccttctgatgattgagaggaagtcaaatt
aggatagg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 814
Transcript exons: 9447475-9448288
CTTGGACACGAAATTGTGATACATCCAAAATGCAAAACAGTACCACTGCTACGCTGATTGCGTC
CTTCTGATGATTGAGAGGAAGTCAAATTAGGATAGGCTGCGGGTGGTTGTTCAGGTTCTTGTTC
CGAAGGTCTGTTTCGATGGGCACAGCGCCAACAAATAAGAGGAATGCTTGCAATGCAGCCAATT
GTGTAACCAATTATCCATGCAAATAAAGGGGCATGCGGATGTTCGTCTCTTGAAAAGACAAGGA
CGAAAATAGCAGCTACAATCTGTACTACATATATGACCAGTTCAAACGAGATCCACAAACCAGA
ATTCCAAATACTTCTTCCATGACCATACATACTATCTCTTCTAGGAAAGGATAAATTTCTGGTG
TTATATGCAGTGGGCGAAGTTGATGCTGAAGACTGTGGTGATGGGGTTTGTGTGCTTGTTGAAG
GCCTATCATCCTGATGTGAATCATCTGAATCACTGTGCAAATCTTGATGACTGGTTGATGTTGA
TGCAGTTTCTCCATGTGATACATTTATAGCATGCTCATGCACATCATCACAGGTAGCATGCTCC
ATGAATATCTTGGATTTATTGATTTCCAGCTTAGCACTAATAAAAAAAGGGAACACACATGCTC
ATCATGGGTAATCATATGCGTTTCACCATCCTTTCAAGTAGCTACCAATGGCTGGCATACTAGT
GTATATACAGCTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTA
TAAACTTCATTTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAG
						
					

						Genomic sequence
						
							CTTGGACACGAAATTGTGATACATCCAAAATGCAAAACAGTACCACTGCTACGCTGATTGCGTC
CTTCTGATGATTGAGAGGAAGTCAAATTAGGATAGGCTGCGGGTGGTTGTTCAGGTTCTTGTTC
CGAAGGTCTGTTTCGATGGGCACAGCGCCAACAAATAAGAGGAATGCTTGCAATGCAGCCAATT
GTGTAACCAATTATCCATGCAAATAAAGGGGCATGCGGATGTTCGTCTCTTGAAAAGACAAGGA
CGAAAATAGCAGCTACAATCTGTACTACATATATGACCAGTTCAAACGAGATCCACAAACCAGA
ATTCCAAATACTTCTTCCATGACCATACATACTATCTCTTCTAGGAAAGGATAAATTTCTGGTG
TTATATGCAGTGGGCGAAGTTGATGCTGAAGACTGTGGTGATGGGGTTTGTGTGCTTGTTGAAG
GCCTATCATCCTGATGTGAATCATCTGAATCACTGTGCAAATCTTGATGACTGGTTGATGTTGA
TGCAGTTTCTCCATGTGATACATTTATAGCATGCTCATGCACATCATCACAGGTAGCATGCTCC
ATGAATATCTTGGATTTATTGATTTCCAGCTTAGCACTAATAAAAAAAGGGAACACACATGCTC
ATCATGGGTAATCATATGCGTTTCACCATCCTTTCAAGTAGCTACCAATGGCTGGCATACTAGT
GTATATACAGCTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTA
TAAACTTCATTTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAG
						
					

			
		

Sequences
Splice junction sequence	CTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTATAAACTTCAT TTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAGcttggacacgaaattgtgatacatccaa aatgcaaaacagtaccactgctacgctgattgcgtccttctgatgattgagaggaagtcaaatt aggatagg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 814 Transcript exons: 9447475-9448288 CTTGGACACGAAATTGTGATACATCCAAAATGCAAAACAGTACCACTGCTACGCTGATTGCGTC CTTCTGATGATTGAGAGGAAGTCAAATTAGGATAGGCTGCGGGTGGTTGTTCAGGTTCTTGTTC CGAAGGTCTGTTTCGATGGGCACAGCGCCAACAAATAAGAGGAATGCTTGCAATGCAGCCAATT GTGTAACCAATTATCCATGCAAATAAAGGGGCATGCGGATGTTCGTCTCTTGAAAAGACAAGGA CGAAAATAGCAGCTACAATCTGTACTACATATATGACCAGTTCAAACGAGATCCACAAACCAGA ATTCCAAATACTTCTTCCATGACCATACATACTATCTCTTCTAGGAAAGGATAAATTTCTGGTG TTATATGCAGTGGGCGAAGTTGATGCTGAAGACTGTGGTGATGGGGTTTGTGTGCTTGTTGAAG GCCTATCATCCTGATGTGAATCATCTGAATCACTGTGCAAATCTTGATGACTGGTTGATGTTGA TGCAGTTTCTCCATGTGATACATTTATAGCATGCTCATGCACATCATCACAGGTAGCATGCTCC ATGAATATCTTGGATTTATTGATTTCCAGCTTAGCACTAATAAAAAAAGGGAACACACATGCTC ATCATGGGTAATCATATGCGTTTCACCATCCTTTCAAGTAGCTACCAATGGCTGGCATACTAGT GTATATACAGCTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTA TAAACTTCATTTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAG
Genomic sequence	CTTGGACACGAAATTGTGATACATCCAAAATGCAAAACAGTACCACTGCTACGCTGATTGCGTC CTTCTGATGATTGAGAGGAAGTCAAATTAGGATAGGCTGCGGGTGGTTGTTCAGGTTCTTGTTC CGAAGGTCTGTTTCGATGGGCACAGCGCCAACAAATAAGAGGAATGCTTGCAATGCAGCCAATT GTGTAACCAATTATCCATGCAAATAAAGGGGCATGCGGATGTTCGTCTCTTGAAAAGACAAGGA CGAAAATAGCAGCTACAATCTGTACTACATATATGACCAGTTCAAACGAGATCCACAAACCAGA ATTCCAAATACTTCTTCCATGACCATACATACTATCTCTTCTAGGAAAGGATAAATTTCTGGTG TTATATGCAGTGGGCGAAGTTGATGCTGAAGACTGTGGTGATGGGGTTTGTGTGCTTGTTGAAG GCCTATCATCCTGATGTGAATCATCTGAATCACTGTGCAAATCTTGATGACTGGTTGATGTTGA TGCAGTTTCTCCATGTGATACATTTATAGCATGCTCATGCACATCATCACAGGTAGCATGCTCC ATGAATATCTTGGATTTATTGATTTCCAGCTTAGCACTAATAAAAAAAGGGAACACACATGCTC ATCATGGGTAATCATATGCGTTTCACCATCCTTTCAAGTAGCTACCAATGGCTGGCATACTAGT GTATATACAGCTGAGAATAAAGTTGATTCTCAGTATAATATACTGAACATTAGTATGTGCATTA TAAACTTCATTTGCTTCATGGAAGAGCCCAGCAGAAGAAGCCTAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.360997993

Conservation Information
Conserved circRNAs	NA
PMCS	0.360997993

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9448018-9447490(+)
9447500-9448210(-)
9448054-9448260(-)
						
						Potential amino acid sequence
						
							MLMHIITGSMLHEYLGFIDFQLSTNKKREHTCSSWVIICVSPSFQVATNGWHTSVYTAENKVDS
QYNILNISMCIINFICFMEEPSRRSLSLDTKL*(+)
MYHNFVSKLRLLLLGSSMKQMKFIMHILMFSILY*(-)
MEHATCDDVHEHAINVSHGETASTSTSHQDLHSDSDDSHQDDRPSTSTQTPSPQSSASTSPTAY
NTRNLSFPRRDSMYGHGRSIWNSGLWISFELVIYVVQIVAAIFVLVFSRDEHPHAPLFAWIIGY
TIGCIASIPLICWRCAHRNRPSEQEPEQPPAAYPNLTSSQSSEGRNQRSSGTVLHFGCITISCP
SLGFFCWALP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9448018-9447490(+) 9447500-9448210(-) 9448054-9448260(-)
Potential amino acid sequence	MLMHIITGSMLHEYLGFIDFQLSTNKKREHTCSSWVIICVSPSFQVATNGWHTSVYTAENKVDS QYNILNISMCIINFICFMEEPSRRSLSLDTKL(+) MYHNFVSKLRLLLLGSSMKQMKFIMHILMFSILY(-) MEHATCDDVHEHAINVSHGETASTSTSHQDLHSDSDDSHQDDRPSTSTQTPSPQSSASTSPTAY NTRNLSFPRRDSMYGHGRSIWNSGLWISFELVIYVVQIVAAIFVLVFSRDEHPHAPLFAWIIGY TIGCIASIPLICWRCAHRNRPSEQEPEQPPAAYPNLTSSQSSEGRNQRSSGTVLHFGCITISCP SLGFFCWALP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os09g0323100_circ_g.2

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