Plant Circular RNA Database

Detail information of AT4G14950_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT4G14950_circ_g.4
					
						ID in PlantcircBase
						ath_circ_030995
					
						Alias
						4:8547034-8547426
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 8547034-8547426  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ; CIRI2
					
						Parent gene
						
							AT4G14950						
					
						Parent gene annotation
						
							Vacuole membrane protein KMS1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G14950_circ_g.1 AT4G14950_circ_g.2 AT4G14950_circ_g.3 AT4G14950_circ_g.5 						
					
						Support reads
						3/1/2
					
						Tissues
						seedling;root;flower
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G14950.3:2
AT4G14950.1:2
AT4G14950.2:2

General Information
CircRNA Name	AT4G14950_circ_g.4
ID in PlantcircBase	ath_circ_030995
Alias	4:8547034-8547426
Organism	Arabidpsis thaliana
Position	chr4: 8547034-8547426 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ; CIRI2
Parent gene	AT4G14950
Parent gene annotation	Vacuole membrane protein KMS1
Parent gene strand	+
Alternative splicing	AT4G14950_circ_g.1 AT4G14950_circ_g.2 AT4G14950_circ_g.3 AT4G14950_circ_g.5
Support reads	3/1/2
Tissues	seedling;root;flower
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G14950.3:2 AT4G14950.1:2 AT4G14950.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCCGGGATTGGCTTCTATGTTGCCTGGACTCACAGCCAAACTCCACGCCATGAAAGAAAAGTAT
ATTGATGCACCATCTCCTGTTCCATCTCACATCAAGgttccaaatcctttgtttgacctcgctg
gaataatgtgtggacaatttggcattccgttttgggaattcttcctggcgaccttaatcggaaa
ggcgatca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 297
Transcript exons: 8547034-8547150,8547247-8547426
GTTCCAAATCCTTTGTTTGACCTCGCTGGAATAATGTGTGGACAATTTGGCATTCCGTTTTGGG
AATTCTTCCTGGCGACCTTAATCGGAAAGGCGATCATCAAAACTCATATACAGACGATATTTAT
CATCTGCGTTTGTAATAACCAACTGCTTGACTGGATGGAGAACGAACTGATCTGGATACTTAGC
CATGTCCCGGGATTGGCTTCTATGTTGCCTGGACTCACAGCCAAACTCCACGCCATGAAAGAAA
AGTATATTGATGCACCATCTCCTGTTCCATCTCACATCAAG
						
						Genomic sequence
						
							GTTCCAAATCCTTTGTTTGACCTCGCTGGAATAATGTGTGGACAATTTGGCATTCCGTTTTGGG
AATTCTTCCTGGCGACCTTAATCGGAAAGGCGATCATCAAAACTCATATACAGGTTCAGAACCT
CGTGTCTCATAGATACTAGTTTAACACCCAAATGGTTTTTTAACTTTGTTTCTTATGTGCTTGT
TTCTCCTTTTTCGTAATGTAGACGATATTTATCATCTGCGTTTGTAATAACCAACTGCTTGACT
GGATGGAGAACGAACTGATCTGGATACTTAGCCATGTCCCGGGATTGGCTTCTATGTTGCCTGG
ACTCACAGCCAAACTCCACGCCATGAAAGAAAAGTATATTGATGCACCATCTCCTGTTCCATCT
CACATCAAG

Sequences
Splice junction sequence	CCCGGGATTGGCTTCTATGTTGCCTGGACTCACAGCCAAACTCCACGCCATGAAAGAAAAGTAT ATTGATGCACCATCTCCTGTTCCATCTCACATCAAGgttccaaatcctttgtttgacctcgctg gaataatgtgtggacaatttggcattccgttttgggaattcttcctggcgaccttaatcggaaa ggcgatca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 297 Transcript exons: 8547034-8547150,8547247-8547426 GTTCCAAATCCTTTGTTTGACCTCGCTGGAATAATGTGTGGACAATTTGGCATTCCGTTTTGGG AATTCTTCCTGGCGACCTTAATCGGAAAGGCGATCATCAAAACTCATATACAGACGATATTTAT CATCTGCGTTTGTAATAACCAACTGCTTGACTGGATGGAGAACGAACTGATCTGGATACTTAGC CATGTCCCGGGATTGGCTTCTATGTTGCCTGGACTCACAGCCAAACTCCACGCCATGAAAGAAA AGTATATTGATGCACCATCTCCTGTTCCATCTCACATCAAG
Genomic sequence	GTTCCAAATCCTTTGTTTGACCTCGCTGGAATAATGTGTGGACAATTTGGCATTCCGTTTTGGG AATTCTTCCTGGCGACCTTAATCGGAAAGGCGATCATCAAAACTCATATACAGGTTCAGAACCT CGTGTCTCATAGATACTAGTTTAACACCCAAATGGTTTTTTAACTTTGTTTCTTATGTGCTTGT TTCTCCTTTTTCGTAATGTAGACGATATTTATCATCTGCGTTTGTAATAACCAACTGCTTGACT GGATGGAGAACGAACTGATCTGGATACTTAGCCATGTCCCGGGATTGGCTTCTATGTTGCCTGG ACTCACAGCCAAACTCCACGCCATGAAAGAAAAGTATATTGATGCACCATCTCCTGTTCCATCT CACATCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.477482198

Conservation Information
Conserved circRNAs	NA
PMCS	0.477482198

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Philips et al., 2020

circRNA

Detail information of AT4G14950_circ_g.4

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