Plant Circular RNA Database

Detail information of Os10g0147400_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os10g0147400_circ_g.2
					
						ID in PlantcircBase
						osa_circ_006214
					
						Alias
						Os_ciR921, 
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 2852155-2852620  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, circseq_cup, find_circ, CIRI-long
					
						Parent gene
						
							Os10g0147400						
					
						Parent gene annotation
						
							Similar to Auxin influx carrier protein. (Os10t0147400-01);Simil
ar to Auxin transporter-like protein 3. (Os10t0147400-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os10g0147400_circ_g.1 						
					
						Support reads
						22/36/37
					
						Tissues
						root/root/shoot, root, pistil, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0147566-00:1
Os10t0147400-01:1
Os10t0147566-00:1

General Information
CircRNA Name	Os10g0147400_circ_g.2
ID in PlantcircBase	osa_circ_006214
Alias	Os_ciR921,
Organism	Oryza sativa
Position	chr10: 2852155-2852620 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, circseq_cup, find_circ, CIRI-long
Parent gene	Os10g0147400
Parent gene annotation	Similar to Auxin influx carrier protein. (Os10t0147400-01);Simil ar to Auxin transporter-like protein 3. (Os10t0147400-03)
Parent gene strand	-
Alternative splicing	Os10g0147400_circ_g.1
Support reads	22/36/37
Tissues	root/root/shoot, root, pistil, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0147566-00:1 Os10t0147400-01:1 Os10t0147566-00:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CGCCGACGCCGACGGCAGCGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTG
AACTTCTGCGGCCGCCACATCGCGTGCATTATCTCCctggcgggattgcggggagcggaaggtg
accatgtaggcgagggaggggatgatgtagacggtgaagctgacgaggagggagccgacggcgg
agttgatg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 170
Transcript exons: 2852155-2852274,2852571-2852620
CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG
AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGGTAGATC
GCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC
Transcript length: 172
Transcript exons: 2852155-2852276,2852571-2852620
CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG
AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGAGGTAGA
TCGCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC
Transcript length: 249
Transcript exons: 2852155-2852230,2852245-2852345,2852549-2852620
CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG
AAGCTGACGAGGGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGAGGAACCAGATGGGG
AGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCAGCACGTACACCGTC
GCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC
Transcript length: 466
Transcript exons: 2852155-2852620
CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG
AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGA
GGAACCAGATGGGGAGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCC
GTGGAGGCCGACGAGCTTCTCCCAGACGAAGTAGAGCGGGGTGCAGGCGAAGCCGAAGGTGATG
AACTGGTGGATGAGCATGAGCACCACCGCGGCGTCGCGCCACGGCGTCCGCGGCAGCAGCGCCA
GCGCGTTCGAGTGCGTCAGCAGCGCGTCCCCGAACGCCCAGTACGCCGCCGACGCCGACGGCAG
CGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCAC
ATCGCGTGCATTATCTCC
						
					

						Genomic sequence
						
							CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG
AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGA
GGAACCAGATGGGGAGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCC
GTGGAGGCCGACGAGCTTCTCCCAGACGAAGTAGAGCGGGGTGCAGGCGAAGCCGAAGGTGATG
AACTGGTGGATGAGCATGAGCACCACCGCGGCGTCGCGCCACGGCGTCCGCGGCAGCAGCGCCA
GCGCGTTCGAGTGCGTCAGCAGCGCGTCCCCGAACGCCCAGTACGCCGCCGACGCCGACGGCAG
CGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCAC
ATCGCGTGCATTATCTCC
						
					

			
		

Sequences
Splice junction sequence	CGCCGACGCCGACGGCAGCGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTG AACTTCTGCGGCCGCCACATCGCGTGCATTATCTCCctggcgggattgcggggagcggaaggtg accatgtaggcgagggaggggatgatgtagacggtgaagctgacgaggagggagccgacggcgg agttgatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 170 Transcript exons: 2852155-2852274,2852571-2852620 CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGGTAGATC GCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC Transcript length: 172 Transcript exons: 2852155-2852276,2852571-2852620 CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGAGGTAGA TCGCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC Transcript length: 249 Transcript exons: 2852155-2852230,2852245-2852345,2852549-2852620 CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG AAGCTGACGAGGGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGAGGAACCAGATGGGG AGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCAGCACGTACACCGTC GCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCACATCGCGTGCATTATCTCC Transcript length: 466 Transcript exons: 2852155-2852620 CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGA GGAACCAGATGGGGAGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCC GTGGAGGCCGACGAGCTTCTCCCAGACGAAGTAGAGCGGGGTGCAGGCGAAGCCGAAGGTGATG AACTGGTGGATGAGCATGAGCACCACCGCGGCGTCGCGCCACGGCGTCCGCGGCAGCAGCGCCA GCGCGTTCGAGTGCGTCAGCAGCGCGTCCCCGAACGCCCAGTACGCCGCCGACGCCGACGGCAG CGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCAC ATCGCGTGCATTATCTCC
Genomic sequence	CTGGCGGGATTGCGGGGAGCGGAAGGTGACCATGTAGGCGAGGGAGGGGATGATGTAGACGGTG AAGCTGACGAGGAGGGAGCCGACGGCGGAGTTGATGGGGCCGAAGAAGGGGAAGATGATGGCGA GGAACCAGATGGGGAGGACGACGGGGAGGCGGGCGGCGGCGCGCTTGCAGAGGGAGGGGCAGCC GTGGAGGCCGACGAGCTTCTCCCAGACGAAGTAGAGCGGGGTGCAGGCGAAGCCGAAGGTGATG AACTGGTGGATGAGCATGAGCACCACCGCGGCGTCGCGCCACGGCGTCCGCGGCAGCAGCGCCA GCGCGTTCGAGTGCGTCAGCAGCGCGTCCCCGAACGCCCAGTACGCCGCCGACGCCGACGGCAG CGTCAGCGTCAGCACGTACACCGTCGCCAGCAGGTAGATCGCCTTGAACTTCTGCGGCCGCCAC ATCGCGTGCATTATCTCC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_008779 hvu_circ_000018* osi_circ_015536 osi_circ_010944 
zma_circ_007737* zma_circ_001454 						
					
						PMCS
						0.527873319

Conservation Information
Conserved circRNAs	osi_circ_008779 hvu_circ_000018* osi_circ_015536 osi_circ_010944 zma_circ_007737* zma_circ_001454
PMCS	0.527873319

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2852615-2852615(-)
						
						Potential amino acid sequence
						
							MHAMWRPQKFKAIYLLATVYVLTLTLPSASAAYWAFGDALLTHSNALALLPRTPWRDAAVVLML
IHQFITFGFACTPLYFVWEKLVGLHGCPSLCKRAAARLPVVLPIWFLAIIFPFFGPINSAVGSL
LVSFTVYIIPSLAYMVTFRSPQSRQGDNARDVAAAEVQGDLPAGDGVRADADAAVGVGGVLGVR
GRAADALERAGAAAADAVARRRGGAHAHPPVHHLRLRLHPALLRLGEARRPPRLPLPLQARRRP
PPRRPPHLVPRHHLPLLRPHQLRRRLPPRQLHRLHHPLPRLHGHLPLPAIPPGR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2852615-2852615(-)
Potential amino acid sequence	MHAMWRPQKFKAIYLLATVYVLTLTLPSASAAYWAFGDALLTHSNALALLPRTPWRDAAVVLML IHQFITFGFACTPLYFVWEKLVGLHGCPSLCKRAAARLPVVLPIWFLAIIFPFFGPINSAVGSL LVSFTVYIIPSLAYMVTFRSPQSRQGDNARDVAAAEVQGDLPAGDGVRADADAAVGVGGVLGVR GRAADALERAGAAAADAVARRRGGAHAHPPVHHLRLRLHPALLRLGEARRPPRLPLPLQARRRP PPRRPPHLVPRHHLPLLRPHQLRRRLPPRQLHRLHHPLPRLHGHLPLPAIPPGR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Ye et al., 2016;Chu et al., 2017; this study

circRNA

Detail information of Os10g0147400_circ_g.2

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