Plant Circular RNA Database

Detail information of orai.009G332500_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.009G332500_circ_g.2
					
						ID in PlantcircBase
						gra_circ_001013
					
						Alias
						Chr09:34586488|34589125
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr09: 34586488-34589125  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.009G332500						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.009G332500_circ_g.1 						
					
						Support reads
						10
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.009G332500.1:7
Gorai.009G332500.2:7

General Information
CircRNA Name	orai.009G332500_circ_g.2
ID in PlantcircBase	gra_circ_001013
Alias	Chr09:34586488\|34589125
Organism	Gossypium raimondii
Position	chrChr09: 34586488-34589125 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.009G332500
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.009G332500_circ_g.1
Support reads	10
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.009G332500.1:7 Gorai.009G332500.2:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCAAACCTTAACCGAAATTAAGGACAAGATATGTTGCTTGACTGACCAGGTGATGCAGAAGGCT
GGCCGTTATGATCCTTCTGGATATTTCCTTATAGAAtgttgacgaccttaaaattctcaaggag
gaagagttagtagaaatggctctcaaggaagcttttaaggtatttgctgatattatcataaagg
atagtatc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 688
Transcript exons: 34586488-34586554,34586661-34586716,34586999-34587161,34
587449-34587537,34587625-34587715,34588666-34588761,3458
9000-34589125
TGTTGACGACCTTAAAATTCTCAAGGAGGAAGAGTTAGTAGAAATGGCTCTCAAGGAAGCTTTT
AAGGAGGATGAGCATGTTGAAAATGCTTCTGAAGGTTTAGAAAACGGCTCCAATTCAGGTTATC
ATACAAGATCCAGAAAGGAACATGCAGTAGAGACTGCTTCAACTTCAATCGAGCCCTCAAATGG
GTGTCCTAGTACTGATTTAGATGGAGCTGCGACAGATAGGAAGAATGGCTCAAAGAAGCAAAAG
AGAAGGAGAATGAATGACCGTCATGTTGAAGATGCATATATCAAAAAGGTTGACGAACTTGCAA
AGATTAAACAAAAGCAGGATGAAGACAAAGCAACAGCAAAGCTACATGCTTTGAATGCCATCTC
CAAGATCTCTGAAGGTTCCATTCCATCGTCTGATAAAATTGAAAGGATGAAATCTCTCCGGTTT
ACGAGTTCTAGTGGAAAGGCAAAGTCGGCAGATGTCAAGGAGCACATACCAGTGTTATACCCTG
AAACTATTCTATGTGTTGAGGTCTACCATAACATACGAAAATGGTCAAAGGTCCAAGAGTTCTT
GGTTCTTGGCCGTCAAACCTTAACCGAAATTAAGGACAAGATATGTTGCTTGACTGACCAGGTG
ATGCAGAAGGCTGGCCGTTATGATCCTTCTGGATATTTCCTTATAGAA
						
					

						Genomic sequence
						
							TGTTGACGACCTTAAAATTCTCAAGGAGGAAGAGTTAGTAGAAATGGCTCTCAAGGAAGCTTTT
AAGGTATTTGCTGATATTATCATAAAGGATAGTATCAAGCTATTAATAAAGTTAGAATTGCTCT
AGTTTTTAGTTTGTTTATTTAATTTATTTATGGATTATATTGTAGGAGGATGAGCATGTTGAAA
ATGCTTCTGAAGGTTTAGAAAACGGCTCCAATTCAGGGTGAGCTATATGCATATTTCTTGCTTG
TTATCTTGTCCTTTTCGGTTGATCTAAAATACAACTTATATTTGAAAATGCTTTTAGATTTGTT
CTAATATATTTTCCGGATTTTGGGATTTATTTTCACTTCTTTATCCTTGAGTAAGCACAATTAC
CTTTTTGGCTGGTAGTTGATGTTATGTCTTAATTTCTTCGCTTTAGATAGGGATCTTCAAGTTT
TTCAGAGCAATACCAATTCATTTTCAGCTTTTGTTTCACTCACTTGTACCATTTGTACTGCAGT
TATCATACAAGATCCAGAAAGGAACATGCAGTAGAGACTGCTTCAACTTCAATCGAGCCCTCAA
ATGGGTGTCCTAGTACTGATTTAGATGGAGCTGCGACAGATAGGAAGAATGGCTCAAAGAAGCA
AAAGAGAAGGAGAATGAATGACCGTCATGTTGAAGTATGGTACTGGTTGTATTGAATTTCTTTT
ACTTTTAAGTACTATAATCTAATCTTAGTGAGTATAATAGTTTAGTAATCTAGCCTTATTTTGT
TCAAACATGACGATTTTTCCTTTTAAACTGTTTGTTTTCCTGCAAAAGTTTTAGTGTTTTCTTG
GCAAGTATATTCGTTTAATCGGATTGCCTATTTTATTTAATGGTCAAAAACAAAAATCACACCT
CACAAACATATCTCGTAATCTTGTTATATTCCTTCTTAACAAAGTTTTCACCATTGCATTTCTA
GGATGCATATATCAAAAAGGTTGACGAACTTGCAAAGATTAAACAAAAGCAGGATGAAGACAAA
GCAACAGCAAAGCTACATGCTTTGAAGTTTGTATTAAGATCTGTCTGTGGTATTCTCTAACTGA
TAAACCTTTTCCTGATCATTGCTGACTTTTGTCTATAAACTTGGTGCAGTGCCATCTCCAAGAT
CTCTGAAGGTTCCATTCCATCGTCTGATAAAATTGAAAGGATGAAATCTCTCCGGTTTACGAGT
TCTAGTGGAAAGGTTTGCCAAAGGTTCCTTTGTCTATTTATTGTAGATGATCTTTTCAAATTCA
TTTTGCTAAAACCTGAAGTCTCTGATTAAACAAATTTGTGGATGAACTTATAAATGTGGTATTC
AAATTTGGTGCATTTATATTATTATATCCTATATTGCTCAGATTCTTCACTTTCCTTGAAATAC
ATAGCTCAAATAAATATTTGTACAATGGTGTGGGGTATGACCTTCCAAGAATCCTCCAAATGCA
TGGAGAAACTTGGAAAAATTGAACATACCCTGCTGGACACATTCTCGTATCCAATGTTTACACC
CAAGCTTGAGTAATATAGCTTATAAACTTTGTTGGTCAAAATATGTCGTCAATCCCTGAACTTT
GGAAAAGTTTGAGATTTAATCCCTCTACTTAAAAAGTTAAAAGTTAAGACTTCTTACTTTTTCG
AGTTAAAAATTGTAGTCCAATCATTGACACCATTAGTATTTTATTTTGGGTCAAAATTTGCATA
CTATCAGATATGCTTACTTAGTTCATAATGATGCTTCTAAATAATGATGCTTCTAAAATTGGTA
TGATAGCATGGATATATTGATGGTAAATTTTGATAGAAAATATTAATGATATCAATGATTGGAC
TAGGGTTTTTAAATTGAAAAAGTAAAGATTTAATTTTTAACTTTTTAAATGCATGGACTAAATC
TCAAATTTCATTCGAGTATAGGGGCTGGCAGCATAATTTGACATACTTTGTTACATAAAGCAAT
GAATTTGAATTGATAATAAATTCATCTGGTGTCCTAATTCAAGCCTTTGGATGCCTTCCCGTAC
AGTTTGAATTAAATTTATTTCTATCTGATCCTTAAAAGATAAGACTAGAATGGCAACCTGTAAG
AGGTTCCAAGGCAGTCAAATTTAGGACTGAAGTGTTCATTCTTCCATTTCTTTCTATGAATGAC
AGGCAAAGTCGGCAGATGTCAAGGAGCACATACCAGTGTTATACCCTGAAACTATTCTATGTGT
TGAGGTCTACCATAACATACGAAAATGGTCAAAGGTATGGTGCACTCAGTTCTAAATCACATAG
CAGTTCATATTCGATTTTTGTTTTTCTTTTATGTTTGTTACAAGTTATGATCAATCAGATCATA
AACTTCTACTGGTTTGTTTCTGATGGTAATGGTAACTCGCTTAGTTCATATAATATCACTGACA
CTTGACTTTCATGATATTATTTCACCATTCATTCCTTTGTCTGATCCTTCTGCTTGTATTGACA
TCTTGAATTGTTGTAGGTCCAAGAGTTCTTGGTTCTTGGCCGTCAAACCTTAACCGAAATTAAG
GACAAGATATGTTGCTTGACTGACCAGGTGATGCAGAAGGCTGGCCGTTATGATCCTTCTGGAT
ATTTCCTTATAGAA
						
					

			
		

Sequences
Splice junction sequence	TCAAACCTTAACCGAAATTAAGGACAAGATATGTTGCTTGACTGACCAGGTGATGCAGAAGGCT GGCCGTTATGATCCTTCTGGATATTTCCTTATAGAAtgttgacgaccttaaaattctcaaggag gaagagttagtagaaatggctctcaaggaagcttttaaggtatttgctgatattatcataaagg atagtatc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 688 Transcript exons: 34586488-34586554,34586661-34586716,34586999-34587161,34 587449-34587537,34587625-34587715,34588666-34588761,3458 9000-34589125 TGTTGACGACCTTAAAATTCTCAAGGAGGAAGAGTTAGTAGAAATGGCTCTCAAGGAAGCTTTT AAGGAGGATGAGCATGTTGAAAATGCTTCTGAAGGTTTAGAAAACGGCTCCAATTCAGGTTATC ATACAAGATCCAGAAAGGAACATGCAGTAGAGACTGCTTCAACTTCAATCGAGCCCTCAAATGG GTGTCCTAGTACTGATTTAGATGGAGCTGCGACAGATAGGAAGAATGGCTCAAAGAAGCAAAAG AGAAGGAGAATGAATGACCGTCATGTTGAAGATGCATATATCAAAAAGGTTGACGAACTTGCAA AGATTAAACAAAAGCAGGATGAAGACAAAGCAACAGCAAAGCTACATGCTTTGAATGCCATCTC CAAGATCTCTGAAGGTTCCATTCCATCGTCTGATAAAATTGAAAGGATGAAATCTCTCCGGTTT ACGAGTTCTAGTGGAAAGGCAAAGTCGGCAGATGTCAAGGAGCACATACCAGTGTTATACCCTG AAACTATTCTATGTGTTGAGGTCTACCATAACATACGAAAATGGTCAAAGGTCCAAGAGTTCTT GGTTCTTGGCCGTCAAACCTTAACCGAAATTAAGGACAAGATATGTTGCTTGACTGACCAGGTG ATGCAGAAGGCTGGCCGTTATGATCCTTCTGGATATTTCCTTATAGAA
Genomic sequence	TGTTGACGACCTTAAAATTCTCAAGGAGGAAGAGTTAGTAGAAATGGCTCTCAAGGAAGCTTTT AAGGTATTTGCTGATATTATCATAAAGGATAGTATCAAGCTATTAATAAAGTTAGAATTGCTCT AGTTTTTAGTTTGTTTATTTAATTTATTTATGGATTATATTGTAGGAGGATGAGCATGTTGAAA ATGCTTCTGAAGGTTTAGAAAACGGCTCCAATTCAGGGTGAGCTATATGCATATTTCTTGCTTG TTATCTTGTCCTTTTCGGTTGATCTAAAATACAACTTATATTTGAAAATGCTTTTAGATTTGTT CTAATATATTTTCCGGATTTTGGGATTTATTTTCACTTCTTTATCCTTGAGTAAGCACAATTAC CTTTTTGGCTGGTAGTTGATGTTATGTCTTAATTTCTTCGCTTTAGATAGGGATCTTCAAGTTT TTCAGAGCAATACCAATTCATTTTCAGCTTTTGTTTCACTCACTTGTACCATTTGTACTGCAGT TATCATACAAGATCCAGAAAGGAACATGCAGTAGAGACTGCTTCAACTTCAATCGAGCCCTCAA ATGGGTGTCCTAGTACTGATTTAGATGGAGCTGCGACAGATAGGAAGAATGGCTCAAAGAAGCA AAAGAGAAGGAGAATGAATGACCGTCATGTTGAAGTATGGTACTGGTTGTATTGAATTTCTTTT ACTTTTAAGTACTATAATCTAATCTTAGTGAGTATAATAGTTTAGTAATCTAGCCTTATTTTGT TCAAACATGACGATTTTTCCTTTTAAACTGTTTGTTTTCCTGCAAAAGTTTTAGTGTTTTCTTG GCAAGTATATTCGTTTAATCGGATTGCCTATTTTATTTAATGGTCAAAAACAAAAATCACACCT CACAAACATATCTCGTAATCTTGTTATATTCCTTCTTAACAAAGTTTTCACCATTGCATTTCTA GGATGCATATATCAAAAAGGTTGACGAACTTGCAAAGATTAAACAAAAGCAGGATGAAGACAAA GCAACAGCAAAGCTACATGCTTTGAAGTTTGTATTAAGATCTGTCTGTGGTATTCTCTAACTGA TAAACCTTTTCCTGATCATTGCTGACTTTTGTCTATAAACTTGGTGCAGTGCCATCTCCAAGAT CTCTGAAGGTTCCATTCCATCGTCTGATAAAATTGAAAGGATGAAATCTCTCCGGTTTACGAGT TCTAGTGGAAAGGTTTGCCAAAGGTTCCTTTGTCTATTTATTGTAGATGATCTTTTCAAATTCA TTTTGCTAAAACCTGAAGTCTCTGATTAAACAAATTTGTGGATGAACTTATAAATGTGGTATTC AAATTTGGTGCATTTATATTATTATATCCTATATTGCTCAGATTCTTCACTTTCCTTGAAATAC ATAGCTCAAATAAATATTTGTACAATGGTGTGGGGTATGACCTTCCAAGAATCCTCCAAATGCA TGGAGAAACTTGGAAAAATTGAACATACCCTGCTGGACACATTCTCGTATCCAATGTTTACACC CAAGCTTGAGTAATATAGCTTATAAACTTTGTTGGTCAAAATATGTCGTCAATCCCTGAACTTT GGAAAAGTTTGAGATTTAATCCCTCTACTTAAAAAGTTAAAAGTTAAGACTTCTTACTTTTTCG AGTTAAAAATTGTAGTCCAATCATTGACACCATTAGTATTTTATTTTGGGTCAAAATTTGCATA CTATCAGATATGCTTACTTAGTTCATAATGATGCTTCTAAATAATGATGCTTCTAAAATTGGTA TGATAGCATGGATATATTGATGGTAAATTTTGATAGAAAATATTAATGATATCAATGATTGGAC TAGGGTTTTTAAATTGAAAAAGTAAAGATTTAATTTTTAACTTTTTAAATGCATGGACTAAATC TCAAATTTCATTCGAGTATAGGGGCTGGCAGCATAATTTGACATACTTTGTTACATAAAGCAAT GAATTTGAATTGATAATAAATTCATCTGGTGTCCTAATTCAAGCCTTTGGATGCCTTCCCGTAC AGTTTGAATTAAATTTATTTCTATCTGATCCTTAAAAGATAAGACTAGAATGGCAACCTGTAAG AGGTTCCAAGGCAGTCAAATTTAGGACTGAAGTGTTCATTCTTCCATTTCTTTCTATGAATGAC AGGCAAAGTCGGCAGATGTCAAGGAGCACATACCAGTGTTATACCCTGAAACTATTCTATGTGT TGAGGTCTACCATAACATACGAAAATGGTCAAAGGTATGGTGCACTCAGTTCTAAATCACATAG CAGTTCATATTCGATTTTTGTTTTTCTTTTATGTTTGTTACAAGTTATGATCAATCAGATCATA AACTTCTACTGGTTTGTTTCTGATGGTAATGGTAACTCGCTTAGTTCATATAATATCACTGACA CTTGACTTTCATGATATTATTTCACCATTCATTCCTTTGTCTGATCCTTCTGCTTGTATTGACA TCTTGAATTGTTGTAGGTCCAAGAGTTCTTGGTTCTTGGCCGTCAAACCTTAACCGAAATTAAG GACAAGATATGTTGCTTGACTGACCAGGTGATGCAGAAGGCTGGCCGTTATGATCCTTCTGGAT ATTTCCTTATAGAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							gar_circ_000176 						
					
						PMCS
						0.425393578

Conservation Information
Conserved circRNAs	gar_circ_000176
PMCS	0.425393578

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							34586531-34586490(+)
						
						Potential amino acid sequence
						
							MALKEAFKEDEHVENASEGLENGSNSGYHTRSRKEHAVETASTSIEPSNGCPSTDLDGAATDRK
NGSKKQKRRRMNDRHVEDAYIKKVDELAKIKQKQDEDKATAKLHALNAISKISEGSIPSSDKIE
RMKSLRFTSSSGKAKSADVKEHIPVLYPETILCVEVYHNIRKWSKVQEFLVLGRQTLTEIKDKI
CCLTDQVMQKAGRYDPSGYFLIEC*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	34586531-34586490(+)
Potential amino acid sequence	MALKEAFKEDEHVENASEGLENGSNSGYHTRSRKEHAVETASTSIEPSNGCPSTDLDGAATDRK NGSKKQKRRRMNDRHVEDAYIKKVDELAKIKQKQDEDKATAKLHALNAISKISEGSIPSSDKIE RMKSLRFTSSSGKAKSADVKEHIPVLYPETILCVEVYHNIRKWSKVQEFLVLGRQTLTEIKDKI CCLTDQVMQKAGRYDPSGYFLIEC*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.009G332500_circ_g.2

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