Plant Circular RNA Database

Detail information of Solyc01g080280.2_circ_g.2

			
						 General Information
					
						CircRNA Name
						Solyc01g080280.2_circ_g.2
					
						ID in PlantcircBase
						sly_circ_000195
					
						Alias
						Slcirc018
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr1: 79530151-79531226  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Solyc01g080280.2						
					
						Parent gene annotation
						
							Glutamine synthetase, chloroplastic/mitochondrial
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Solyc01g080280.2_circ_g.1 Solyc01g080280.2_circ_g.3 						
					
						Support reads
						3/118
					
						Tissues
						fruit/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc01g080280.2.1:5

General Information
CircRNA Name	Solyc01g080280.2_circ_g.2
ID in PlantcircBase	sly_circ_000195
Alias	Slcirc018
Organism	Solanum lycopersicum
Position	chr1: 79530151-79531226 JBrowse»
Reference genome	SL2.50.38
Type	ue-circRNA
Identification method	CIRI
Parent gene	Solyc01g080280.2
Parent gene annotation	Glutamine synthetase, chloroplastic/mitochondrial
Parent gene strand	+
Alternative splicing	Solyc01g080280.2_circ_g.1 Solyc01g080280.2_circ_g.3
Support reads	3/118
Tissues	fruit/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc01g080280.2.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAACAAACGCCATAAAGCTGCTCAAAT
TTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATGttggttaggaggtgaacatggctcagat
cctggctccgtctgcacaatggcagatgagaatgacaaagagctcaaccgatgctagtcccttg
acttcaaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 571
Transcript exons: 79530151-79530421,79530585-79530624,79530726-79530829,79
530971-79531019,79531120-79531226
TTGGTTAGGAGGTGAACATGGCTCAGATCCTGGCTCCGTCTGCACAATGGCAGATGAGAATGAC
AAAGAGCTCAACCGATGCTAGTCCCTTGACTTCAAAGATGTGGAGCTCTGTGGTGCTGAAGCAG
AACAAAAGACACGCTCTTAAAAGCTCTGCCAAATTTAGAGTTTTTGCCCTACAGTCTGACAATG
GCACCGTGAACAGAGTGGAACAGCTGCTAAACTTGGACGTAACTCCATACACTGATAAGATCAT
TGCTGAATATATTTGGATCGGAGGGACTGGAATTGACATGCGCAGTAAATCAAGGACTATTTCG
AAACCAGTCAAGGATGCTTCTGAGCTCCCAAAGTGGAACTACGATGGATCAAGTACTGGACAAG
CACCTGGAGAAGACAGTGAAGTCATTCTATATCCTCAGGCAATATTCAAAGACCCTTTCCGTGG
TGGTAACAACATCTTGGTTATCTGTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAAC
AAACGCCATAAAGCTGCTCAAATTTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATG
						
					

						Genomic sequence
						
							TTGGTTAGGAGGTGAACATGGCTCAGATCCTGGCTCCGTCTGCACAATGGCAGATGAGAATGAC
AAAGAGCTCAACCGATGCTAGTCCCTTGACTTCAAAGATGTGGAGCTCTGTGGTGCTGAAGCAG
AACAAAAGACACGCTCTTAAAAGCTCTGCCAAATTTAGAGTTTTTGCCCTACAGTCTGACAATG
GCACCGTGAACAGAGTGGAACAGCTGCTAAACTTGGACGTAACTCCATACACTGATAAGATCAT
TGCTGAATATATTTGGTATATTATTCTTTGCTATATGCATCTCATACTGTTTACTCAACATTGT
CACTTGAAGATTTGTAGATCGTGTCTGTCATTTCTTCACTTTCACTACCTTTACGAATGCCTAC
ATCTAGTTCTTTATGGATAGACTGACCTTGTGACATTTCCTTCAAAACAGGATCGGAGGGACTG
GAATTGACATGCGCAGTAAATCAAGGGTACATAGTGCTGCTTCTCTAATTAACCAACAAAAGTT
TAGGAAACTGAATTTGGATTATCTCATGTATTTTCTATTAATTGTTTCCTTTTGAATTTTCAGA
CTATTTCGAAACCAGTCAAGGATGCTTCTGAGCTCCCAAAGTGGAACTACGATGGATCAAGTAC
TGGACAAGCACCTGGAGAAGACAGTGAAGTCATTCTATAGTAAGATCAGCAGCTGAATTTTTTT
TTCTTTCTTTTTTGAAGAATTTTCCTTTCTTTCTTTTATTCCTTATTTTATTGTAAAAAAAATT
CTTCCTTTCATTTTTCCCCATTCTAAAGCTAACCTTGATCATTTAACCTCAGTCCTCAGGCAAT
ATTCAAAGACCCTTTCCGTGGTGGTAACAACATCTTGGTGAGTTGTTTAGTAAACAATACTGAT
TCATGATGTTTTACTGAATCTTTAATCTCATTGGTTTTAAGTTTGGCACTATTGTTTGTCTAAA
TTGAAACAGGTTATCTGTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAACAAACGCC
ATAAAGCTGCTCAAATTTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATG
						
					

			
		

Sequences
Splice junction sequence	GTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAACAAACGCCATAAAGCTGCTCAAAT TTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATGttggttaggaggtgaacatggctcagat cctggctccgtctgcacaatggcagatgagaatgacaaagagctcaaccgatgctagtcccttg acttcaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 571 Transcript exons: 79530151-79530421,79530585-79530624,79530726-79530829,79 530971-79531019,79531120-79531226 TTGGTTAGGAGGTGAACATGGCTCAGATCCTGGCTCCGTCTGCACAATGGCAGATGAGAATGAC AAAGAGCTCAACCGATGCTAGTCCCTTGACTTCAAAGATGTGGAGCTCTGTGGTGCTGAAGCAG AACAAAAGACACGCTCTTAAAAGCTCTGCCAAATTTAGAGTTTTTGCCCTACAGTCTGACAATG GCACCGTGAACAGAGTGGAACAGCTGCTAAACTTGGACGTAACTCCATACACTGATAAGATCAT TGCTGAATATATTTGGATCGGAGGGACTGGAATTGACATGCGCAGTAAATCAAGGACTATTTCG AAACCAGTCAAGGATGCTTCTGAGCTCCCAAAGTGGAACTACGATGGATCAAGTACTGGACAAG CACCTGGAGAAGACAGTGAAGTCATTCTATATCCTCAGGCAATATTCAAAGACCCTTTCCGTGG TGGTAACAACATCTTGGTTATCTGTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAAC AAACGCCATAAAGCTGCTCAAATTTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATG
Genomic sequence	TTGGTTAGGAGGTGAACATGGCTCAGATCCTGGCTCCGTCTGCACAATGGCAGATGAGAATGAC AAAGAGCTCAACCGATGCTAGTCCCTTGACTTCAAAGATGTGGAGCTCTGTGGTGCTGAAGCAG AACAAAAGACACGCTCTTAAAAGCTCTGCCAAATTTAGAGTTTTTGCCCTACAGTCTGACAATG GCACCGTGAACAGAGTGGAACAGCTGCTAAACTTGGACGTAACTCCATACACTGATAAGATCAT TGCTGAATATATTTGGTATATTATTCTTTGCTATATGCATCTCATACTGTTTACTCAACATTGT CACTTGAAGATTTGTAGATCGTGTCTGTCATTTCTTCACTTTCACTACCTTTACGAATGCCTAC ATCTAGTTCTTTATGGATAGACTGACCTTGTGACATTTCCTTCAAAACAGGATCGGAGGGACTG GAATTGACATGCGCAGTAAATCAAGGGTACATAGTGCTGCTTCTCTAATTAACCAACAAAAGTT TAGGAAACTGAATTTGGATTATCTCATGTATTTTCTATTAATTGTTTCCTTTTGAATTTTCAGA CTATTTCGAAACCAGTCAAGGATGCTTCTGAGCTCCCAAAGTGGAACTACGATGGATCAAGTAC TGGACAAGCACCTGGAGAAGACAGTGAAGTCATTCTATAGTAAGATCAGCAGCTGAATTTTTTT TTCTTTCTTTTTTGAAGAATTTTCCTTTCTTTCTTTTATTCCTTATTTTATTGTAAAAAAAATT CTTCCTTTCATTTTTCCCCATTCTAAAGCTAACCTTGATCATTTAACCTCAGTCCTCAGGCAAT ATTCAAAGACCCTTTCCGTGGTGGTAACAACATCTTGGTGAGTTGTTTAGTAAACAATACTGAT TCATGATGTTTTACTGAATCTTTAATCTCATTGGTTTTAAGTTTGGCACTATTGTTTGTCTAAA TTGAAACAGGTTATCTGTGATGCCTACACACCAGCTGGAGAGCCAATTCCTACAAACAAACGCC ATAAAGCTGCTCAAATTTTTAGCGACCCAAAAGTTGCAGCTCAAGTTCCATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.665829801

Conservation Information
Conserved circRNAs	NA
PMCS	0.665829801

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							79530749-79530155(+)
79530169-79530232(+)
						
						Potential amino acid sequence
						
							MLLSSQSGTTMDQVLDKHLEKTVKSFYILRQYSKTLSVVVTTSWLSVMPTHQLESQFLQTNAIK
LLKFLATQKLQLKFHVG*(+)
MAQILAPSAQWQMRMTKSSTDASPLTSKMWSSVVLKQNKRHALKSSAKFRVFALQSDNGTVNRV
EQLLNLDVTPYTDKIIAEYIWIGGTGIDMRSKSRTISKPVKDASELPKWNYDGSSTGQAPGEDS
EVILYPQAIFKDPFRGGNNILVICDAYTPAGEPIPTNKRHKAAQIFSDPKVAAQVPCWLGGEHG
SDPGSVCTMADENDKELNRC*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	79530749-79530155(+) 79530169-79530232(+)
Potential amino acid sequence	MLLSSQSGTTMDQVLDKHLEKTVKSFYILRQYSKTLSVVVTTSWLSVMPTHQLESQFLQTNAIK LLKFLATQKLQLKFHVG(+) MAQILAPSAQWQMRMTKSSTDASPLTSKMWSSVVLKQNKRHALKSSAKFRVFALQSDNGTVNRV EQLLNLDVTPYTDKIIAEYIWIGGTGIDMRSKSRTISKPVKDASELPKWNYDGSSTGQAPGEDS EVILYPQAIFKDPFRGGNNILVICDAYTPAGEPIPTNKRHKAAQIFSDPKVAAQVPCWLGGEHG SDPGSVCTMADENDKELNRC(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zuo et al., 2016; Wang et al., 2018b

circRNA

Detail information of Solyc01g080280.2_circ_g.2

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