Plant Circular RNA Database

Detail information of Os08g0300300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os08g0300300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_036626
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 12351435-12352088  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ie-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0300333						
					
						Parent gene annotation
						
							Hypothetical protein. (Os08t0300333-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os08g0300300_circ_g.2 Os08g0300300_circ_g.3 Os08g0300300_circ_g.4 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0300300-01:2
Os08t0300333-00:2
Os08t0300300-01:2

General Information
CircRNA Name	Os08g0300300_circ_g.1
ID in PlantcircBase	osa_circ_036626
Alias	NA
Organism	Oryza sativa
Position	chr8: 12351435-12352088 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ie-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0300333
Parent gene annotation	Hypothetical protein. (Os08t0300333-00)
Parent gene strand	-
Alternative splicing	Os08g0300300_circ_g.2 Os08g0300300_circ_g.3 Os08g0300300_circ_g.4
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0300300-01:2 Os08t0300333-00:2 Os08t0300300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTCGCCGGCGGCCGGCGGGTCGGGGATCCCGGAGGTGAAGGCCTACCTCAACGGCGTGGACGCG
CCAAACATCTTCTCGCTCAGGACACTCGCTGTCAAGgataatagagaacgacctgttcgatcag
aactggaggtcgagagggaaagccgaccaggtgcggtacgtggtgctcaagtggaccttctgct
tcgccatc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 379
Transcript exons: 12351435-12351635,12351911-12352088
GATAATAGAGAACGACCTGTTCGATCAGAACTGGAGGTCGAGAGGGAAAGCCGACCAGGTGCGG
TACGTGGTGCTCAAGTGGACCTTCTGCTTCGCCATCGGCATCATCACCGGCATCGCCGGCTTCG
TCATCAACCTCGCCGTCGAGAATGTCGCCGGCCTCAAGCACACCGCCGTGTCCGCCCTCATGGA
GTCCAGCAGCTATTGGACAGCGTTCTGGCTGTTCGCCGGAACCAACCTGGCCCTGCTGCTGTTC
GCGTCGTCCATCACGGCGTTCGTGTCGCCGGCGGCCGGCGGGTCGGGGATCCCGGAGGTGAAGG
CCTACCTCAACGGCGTGGACGCGCCAAACATCTTCTCGCTCAGGACACTCGCTGTCAAG
						
						Genomic sequence
						
							GATAATAGAGAACGACCTGTTCGATCAGAACTGGAGGTCGAGAGGGAAAGCCGACCAGGTGCGG
TACGTGGTGCTCAAGTGGACCTTCTGCTTCGCCATCGGCATCATCACCGGCATCGCCGGCTTCG
TCATCAACCTCGCCGTCGAGAATGTCGCCGGCCTCAAGCACACCGCCGTGTCCGCCCTCATGGA
GTCCAGCAGGTTCTGCTCATTGCCTCCTATTGCCTTGTCCAAGAAAAAAGTAATGTTGCTTTTG
CTGAGTACGACAAATTCACTCATGCTTGTCTCCTGCTTTGTCTATTATTATCCCGTACTCTTTT
TGTCCCTGATCAGATCAATTCTTGTTCAAATCTGAGAATGCTATCTGTCAAGTGATCTAGAATT
CTGAACCCTCAAAATTGACTGAATTTTTGGAACAAAGATCAATTTCTGTCTGTTACTCCGAGTC
TGAAGTCTGAAACTCATGTCGGATGCAGCTATTGGACAGCGTTCTGGCTGTTCGCCGGAACCAA
CCTGGCCCTGCTGCTGTTCGCGTCGTCCATCACGGCGTTCGTGTCGCCGGCGGCCGGCGGGTCG
GGGATCCCGGAGGTGAAGGCCTACCTCAACGGCGTGGACGCGCCAAACATCTTCTCGCTCAGGA
CACTCGCTGTCAAG

Sequences
Splice junction sequence	GTCGCCGGCGGCCGGCGGGTCGGGGATCCCGGAGGTGAAGGCCTACCTCAACGGCGTGGACGCG CCAAACATCTTCTCGCTCAGGACACTCGCTGTCAAGgataatagagaacgacctgttcgatcag aactggaggtcgagagggaaagccgaccaggtgcggtacgtggtgctcaagtggaccttctgct tcgccatc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 379 Transcript exons: 12351435-12351635,12351911-12352088 GATAATAGAGAACGACCTGTTCGATCAGAACTGGAGGTCGAGAGGGAAAGCCGACCAGGTGCGG TACGTGGTGCTCAAGTGGACCTTCTGCTTCGCCATCGGCATCATCACCGGCATCGCCGGCTTCG TCATCAACCTCGCCGTCGAGAATGTCGCCGGCCTCAAGCACACCGCCGTGTCCGCCCTCATGGA GTCCAGCAGCTATTGGACAGCGTTCTGGCTGTTCGCCGGAACCAACCTGGCCCTGCTGCTGTTC GCGTCGTCCATCACGGCGTTCGTGTCGCCGGCGGCCGGCGGGTCGGGGATCCCGGAGGTGAAGG CCTACCTCAACGGCGTGGACGCGCCAAACATCTTCTCGCTCAGGACACTCGCTGTCAAG
Genomic sequence	GATAATAGAGAACGACCTGTTCGATCAGAACTGGAGGTCGAGAGGGAAAGCCGACCAGGTGCGG TACGTGGTGCTCAAGTGGACCTTCTGCTTCGCCATCGGCATCATCACCGGCATCGCCGGCTTCG TCATCAACCTCGCCGTCGAGAATGTCGCCGGCCTCAAGCACACCGCCGTGTCCGCCCTCATGGA GTCCAGCAGGTTCTGCTCATTGCCTCCTATTGCCTTGTCCAAGAAAAAAGTAATGTTGCTTTTG CTGAGTACGACAAATTCACTCATGCTTGTCTCCTGCTTTGTCTATTATTATCCCGTACTCTTTT TGTCCCTGATCAGATCAATTCTTGTTCAAATCTGAGAATGCTATCTGTCAAGTGATCTAGAATT CTGAACCCTCAAAATTGACTGAATTTTTGGAACAAAGATCAATTTCTGTCTGTTACTCCGAGTC TGAAGTCTGAAACTCATGTCGGATGCAGCTATTGGACAGCGTTCTGGCTGTTCGCCGGAACCAA CCTGGCCCTGCTGCTGTTCGCGTCGTCCATCACGGCGTTCGTGTCGCCGGCGGCCGGCGGGTCG GGGATCCCGGAGGTGAAGGCCTACCTCAACGGCGTGGACGCGCCAAACATCTTCTCGCTCAGGA CACTCGCTGTCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.263630708

Conservation Information
Conserved circRNAs	NA
PMCS	0.263630708

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12351623-12351436(+)
						
						Potential amino acid sequence
						
							MESSSYWTAFWLFAGTNLALLLFASSITAFVSPAAGGSGIPEVKAYLNGVDAPNIFSLRTLAVK
DNRERPVRSELEVERESRPGAVRGAQVDLLLRHRHHHRHRRLRHQPRRRECRRPQAHRRVRPHG
VQQLLDSVLAVRRNQPGPAAVRVVHHGVRVAGGRRVGDPGGEGLPQRRGRAKHLLAQDTRCQG*
(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12351623-12351436(+)
Potential amino acid sequence	MESSSYWTAFWLFAGTNLALLLFASSITAFVSPAAGGSGIPEVKAYLNGVDAPNIFSLRTLAVK DNRERPVRSELEVERESRPGAVRGAQVDLLLRHRHHHRHRRLRHQPRRRECRRPQAHRRVRPHG VQQLLDSVLAVRRNQPGPAAVRVVHHGVRVAGGRRVGDPGGEGLPQRRGRAKHLLAQDTRCQG* (+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0300300_circ_g.1

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