Plant Circular RNA Database

Detail information of Os05g0295100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0295100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_027264
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 13027806-13045863  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							Os05g0295100						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os05t0295100-01);Protein of unknow
n function DUF1253 family protein. (Os05t0295100-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0295100_circ_g.1 Os05g0295100_circ_g.3 Os05g0295100_circ_g.4 Os05g0295100_circ_g.5 						
					
						Support reads
						2
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0295100-02:21
Os05t0295100-01:10

General Information
CircRNA Name	Os05g0295100_circ_g.2
ID in PlantcircBase	osa_circ_027264
Alias	NA
Organism	Oryza sativa
Position	chr5: 13027806-13045863 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	Os05g0295100
Parent gene annotation	Hypothetical conserved gene. (Os05t0295100-01);Protein of unknow n function DUF1253 family protein. (Os05t0295100-02)
Parent gene strand	+
Alternative splicing	Os05g0295100_circ_g.1 Os05g0295100_circ_g.3 Os05g0295100_circ_g.4 Os05g0295100_circ_g.5
Support reads	2
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0295100-02:21 Os05t0295100-01:10

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAAAAATTATCTGGGGCCTTTCTTTCAGCTTGTAAACATGCTCAGTGAATCAGAAAACAGGAAA
TGCACCGTCTTTTTTTCACGTCTGGATCTTCTGAAGggcacaagaggcgcccgaagaggacaag
gccctcggggaagggaggaggacccgagccgcgcgaggagaagcgcaagaggcgagagctcgat
ggtgtcgg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	AAAAAATTATCTGGGGCCTTTCTTTCAGCTTGTAAACATGCTCAGTGAATCAGAAAACAGGAAA TGCACCGTCTTTTTTTCACGTCTGGATCTTCTGAAGggcacaagaggcgcccgaagaggacaag gccctcggggaagggaggaggacccgagccgcgcgaggagaagcgcaagaggcgagagctcgat ggtgtcgg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.087143697

Conservation Information
Conserved circRNAs	NA
PMCS	0.087143697

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							13045828-13027940(+)
13028387-13027940(+)
13028389-13045807(-)
13028864-13045845(-)
						
					

						Potential amino acid sequence
						
							MHRLFFTSGSSEGHKRRPKRTRPSGKGGGPEPREEKRKRRELDGVGLAEESPPPGSSLGKILST
ESGAIGLEQSDEDDMEDEHTVPGDDETNDMINNKDDAFDEMEASCSFHRHVSRIITNEEVNALL
KQKCKFKWEVPAEDIPKSKWVGTGEKMEGAYVDLIGGVKGKLRDHWQNTLSDQLNSRLNFFSLC
SSYRDIMHCNKKPFYLKGGSTVDSSTMDSYLMHALNHINRTREIVVKNDAKLRSDPSKDILDDN
SFLDQGFTRPKVLFLLPLKSIARRVVKRLIQLSPLSQKDIIAKFEGKFGESDDEVEEPVQSNKP
ADFDLLFAGDTDDEFLFGIKYTKKSMNLFSNFYSSDIIVASPLALIKKINGVDDNGKEPAKERD
FDFLSSIEIVVVDHADVILMQSWSHLYAVFEQLNQLPSKEHGTNVMRIRPWYLDQHARYYRQTI
LLSSYLTPEMNALFNGLCLNYEGKVKLVTEFKGVLSKIQFEAPQVYERFDASSITEVDDARFDH
FCKKVYPKIQESDEGRVLLFVSSYFEYIRIRNFLKSQEASFCRIGETTSQQDISRARLWFFEGK
KKILLYSERSHFYHRYKIRGAHHLIIYSLPGRKEFYPELVNMLSESENRKCTVFFSRLDLLKGT
RGARRGQGPRGREEDPSRARRSARGESSMVSAWRRSRRRRVPA*(+)
MEDEHTVPGDDETNDMINNKDDAFDEMEASCSFHRHVSRIITNEEVNALLKQKCKFKWEVPAED
IPKSKWVGTGEKMEGAYVDLIGGVKGKLRDHWQNTLSDQLNSRLNFFSLCSSYRDIMHCNKKPF
YLKGGSTVDSSTMDSYLMHALNHINRTREIVVKNDAKLRSDPSKDILDDNSFLDQGFTRPKVLF
LLPLKSIARRVVKRLIQLSPLSQKDIIAKFEGKFGESDDEVEEPVQSNKPADFDLLFAGDTDDE
FLFGIKYTKKSMNLFSNFYSSDIIVASPLALIKKINGVDDNGKEPAKERDFDFLSSIEIVVVDH
ADVILMQSWSHLYAVFEQLNQLPSKEHGTNVMRIRPWYLDQHARYYRQTILLSSYLTPEMNALF
NGLCLNYEGKVKLVTEFKGVLSKIQFEAPQVYERFDASSITEVDDARFDHFCKKVYPKIQESDE
GRVLLFVSSYFEYIRIRNFLKSQEASFCRIGETTSQQDISRARLWFFEGKKKILLYSERSHFYH
RYKIRGAHHLIIYSLPGRKEFYPELVNMLSESENRKCTVFFSRLDLLKGTRGARRGQGPRGREE
DPSRARRSARGESSMVSAWRRSRRRRVPA*(+)
MSSSSLCSRPIAPDSVLKILPRLEPGGGDSSARPTPSSSRLLRFSSRGSGPPPFPEGLVLFGRL
LCPSEDPDVKKRRCISCFLIH*(-)
MNMKPPFHQKHHLYCLSYHLSHRHQEQCVHLPCHLHHSALGLLLQIRYLRFCLGWNPAAATPPP
GRHHRALASCASPRAARVLLPSPRALSSSGASCALQKIQT*(-)
						
					

						Sponge-miRNAs
						
							osa-miR1862f 						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	13045828-13027940(+) 13028387-13027940(+) 13028389-13045807(-) 13028864-13045845(-)
Potential amino acid sequence	MHRLFFTSGSSEGHKRRPKRTRPSGKGGGPEPREEKRKRRELDGVGLAEESPPPGSSLGKILST ESGAIGLEQSDEDDMEDEHTVPGDDETNDMINNKDDAFDEMEASCSFHRHVSRIITNEEVNALL KQKCKFKWEVPAEDIPKSKWVGTGEKMEGAYVDLIGGVKGKLRDHWQNTLSDQLNSRLNFFSLC SSYRDIMHCNKKPFYLKGGSTVDSSTMDSYLMHALNHINRTREIVVKNDAKLRSDPSKDILDDN SFLDQGFTRPKVLFLLPLKSIARRVVKRLIQLSPLSQKDIIAKFEGKFGESDDEVEEPVQSNKP ADFDLLFAGDTDDEFLFGIKYTKKSMNLFSNFYSSDIIVASPLALIKKINGVDDNGKEPAKERD FDFLSSIEIVVVDHADVILMQSWSHLYAVFEQLNQLPSKEHGTNVMRIRPWYLDQHARYYRQTI LLSSYLTPEMNALFNGLCLNYEGKVKLVTEFKGVLSKIQFEAPQVYERFDASSITEVDDARFDH FCKKVYPKIQESDEGRVLLFVSSYFEYIRIRNFLKSQEASFCRIGETTSQQDISRARLWFFEGK KKILLYSERSHFYHRYKIRGAHHLIIYSLPGRKEFYPELVNMLSESENRKCTVFFSRLDLLKGT RGARRGQGPRGREEDPSRARRSARGESSMVSAWRRSRRRRVPA(+) MEDEHTVPGDDETNDMINNKDDAFDEMEASCSFHRHVSRIITNEEVNALLKQKCKFKWEVPAED IPKSKWVGTGEKMEGAYVDLIGGVKGKLRDHWQNTLSDQLNSRLNFFSLCSSYRDIMHCNKKPF YLKGGSTVDSSTMDSYLMHALNHINRTREIVVKNDAKLRSDPSKDILDDNSFLDQGFTRPKVLF LLPLKSIARRVVKRLIQLSPLSQKDIIAKFEGKFGESDDEVEEPVQSNKPADFDLLFAGDTDDE FLFGIKYTKKSMNLFSNFYSSDIIVASPLALIKKINGVDDNGKEPAKERDFDFLSSIEIVVVDH ADVILMQSWSHLYAVFEQLNQLPSKEHGTNVMRIRPWYLDQHARYYRQTILLSSYLTPEMNALF NGLCLNYEGKVKLVTEFKGVLSKIQFEAPQVYERFDASSITEVDDARFDHFCKKVYPKIQESDE GRVLLFVSSYFEYIRIRNFLKSQEASFCRIGETTSQQDISRARLWFFEGKKKILLYSERSHFYH RYKIRGAHHLIIYSLPGRKEFYPELVNMLSESENRKCTVFFSRLDLLKGTRGARRGQGPRGREE DPSRARRSARGESSMVSAWRRSRRRRVPA(+) MSSSSLCSRPIAPDSVLKILPRLEPGGGDSSARPTPSSSRLLRFSSRGSGPPPFPEGLVLFGRL LCPSEDPDVKKRRCISCFLIH(-) MNMKPPFHQKHHLYCLSYHLSHRHQEQCVHLPCHLHHSALGLLLQIRYLRFCLGWNPAAATPPP GRHHRALASCASPRAARVLLPSPRALSSSGASCALQKIQT(-)
Sponge-miRNAs	osa-miR1862f
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0295100_circ_g.2

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