Plant Circular RNA Database

Detail information of Solyc08g074290.2_circ_g.1

			
						 General Information
					
						CircRNA Name
						Solyc08g074290.2_circ_g.1
					
						ID in PlantcircBase
						sly_circ_002621
					
						Alias
						8:55573097|55574235, SL3.0ch08:58527639|58528777
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr8: 58407097-58408235  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI, circseq_cup
					
						Parent gene
						
							Solyc08g074290.2						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						3/15
					
						Tissues
						fruit
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc08g074290.2.1:3

General Information
CircRNA Name	Solyc08g074290.2_circ_g.1
ID in PlantcircBase	sly_circ_002621
Alias	8:55573097\|55574235, SL3.0ch08:58527639\|58528777
Organism	Solanum lycopersicum
Position	chr8: 58407097-58408235 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	CIRI, circseq_cup
Parent gene	Solyc08g074290.2
Parent gene annotation	protein_coding
Parent gene strand	+
Alternative splicing	NA
Support reads	3/15
Tissues	fruit
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc08g074290.2.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCCGTAACTCCACATATT
GTCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAGtctcttctggagaagcattgggaggagc
acggaaaacctgctatgaacacaatgatacagaaggtacatgtcttgttgctactggatcctgt
ttaactat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 291
Transcript exons: 58407097-58407159,58407266-58407328,58408071-58408235
TCTCTTCTGGAGAAGCATTGGGAGGAGCACGGAAAACCTGCTATGAACACAATGATACAGAAGG
CAGTTGAGAAGAAGGCTCAAGCTGAGGTATGGGCTGCGCCACATATAGAAACAGTCAAAACAAA
ATGGATGCCTGCTGTTGAGGAACAATGGGTGGTAATGACCACTAACCTCAAGCCACAAATGGAA
TTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCCGTAACTCCACATATTG
TCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAG
						
						Genomic sequence
						
							TCTCTTCTGGAGAAGCATTGGGAGGAGCACGGAAAACCTGCTATGAACACAATGATACAGAAGG
TACATGTCTTGTTGCTACTGGATCCTGTTTAACTATTGATTATTGAACGAATTCTTGTTTGAGT
TACTATTCTATCCTGTTTACCCTTTTTCACTTGCTTCTTAGGCAGTTGAGAAGAAGGCTCAAGC
TGAGGTATGGGCTGCGCCACATATAGAAACAGTCAAAACAGTAAGTCAGCTTTGCATCTTATTA
CCATATTCAATTAAATTAATTTATTGGATGAGTTCTCGGCACTTACGAAAGCCAGAAATGCTAT
GATGTCAAATACTTTGGAATGTCCCAAAATGGAAAAGAGTGTCATAAATATTGGAAAAAGATGG
AGTAGGCCCTTCTTTTAGGGTATACCACTTCTTCCTTCTCTACAACATCCCAGTGTCTCACATT
TCCATTCTCTCTGTTTCTCATCATTTTGTGTTAACCCAACCCATTTATTAACTCACCCCTATTT
AACTTGCCCAATTCCAGCCCAACCCACCCATTTGGCACCTCTAGGTATACATTAACGAGTAAGT
TATTACATTAAGTCCTCATCTCTTCATGGAAATAAGTACTTAAACAATAAAACTAAACTAATTT
GAGATTTCCTGTTAACAAAGCAGTAATGGTACCTTGCTAGATAAAACTGTTAAAATGGTAGAGT
ATCTTTGTTTCTTCCCAAGAGGTGAGTTCTCTTATTACACTCACCAGACATCCGGGGGAGGAAA
ATCAAGAGTTAGTAGGACAAGCTAAAGGTGGGCAGTAAGATGACTTCCAGATCTGGGAAGAATC
TCTATTAGTTTTTTTTACATGTAGATACAATTATCTACTTCAAACATTTAATTTACTCCTAAAG
CTAGTTTTTTGTGCTGTATTTGTTATCAAGATAGAGGATCTTAATAATGTCTCGTGTTTGCTGT
TCTACTTTTTGAAGAAATGGATGCCTGCTGTTGAGGAACAATGGGTGGTAATGACCACTAACCT
CAAGCCACAAATGGAATTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCC
GTAACTCCACATATTGTCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAG

Sequences
Splice junction sequence	ATTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCCGTAACTCCACATATT GTCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAGtctcttctggagaagcattgggaggagc acggaaaacctgctatgaacacaatgatacagaaggtacatgtcttgttgctactggatcctgt ttaactat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 291 Transcript exons: 58407097-58407159,58407266-58407328,58408071-58408235 TCTCTTCTGGAGAAGCATTGGGAGGAGCACGGAAAACCTGCTATGAACACAATGATACAGAAGG CAGTTGAGAAGAAGGCTCAAGCTGAGGTATGGGCTGCGCCACATATAGAAACAGTCAAAACAAA ATGGATGCCTGCTGTTGAGGAACAATGGGTGGTAATGACCACTAACCTCAAGCCACAAATGGAA TTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCCGTAACTCCACATATTG TCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAG
Genomic sequence	TCTCTTCTGGAGAAGCATTGGGAGGAGCACGGAAAACCTGCTATGAACACAATGATACAGAAGG TACATGTCTTGTTGCTACTGGATCCTGTTTAACTATTGATTATTGAACGAATTCTTGTTTGAGT TACTATTCTATCCTGTTTACCCTTTTTCACTTGCTTCTTAGGCAGTTGAGAAGAAGGCTCAAGC TGAGGTATGGGCTGCGCCACATATAGAAACAGTCAAAACAGTAAGTCAGCTTTGCATCTTATTA CCATATTCAATTAAATTAATTTATTGGATGAGTTCTCGGCACTTACGAAAGCCAGAAATGCTAT GATGTCAAATACTTTGGAATGTCCCAAAATGGAAAAGAGTGTCATAAATATTGGAAAAAGATGG AGTAGGCCCTTCTTTTAGGGTATACCACTTCTTCCTTCTCTACAACATCCCAGTGTCTCACATT TCCATTCTCTCTGTTTCTCATCATTTTGTGTTAACCCAACCCATTTATTAACTCACCCCTATTT AACTTGCCCAATTCCAGCCCAACCCACCCATTTGGCACCTCTAGGTATACATTAACGAGTAAGT TATTACATTAAGTCCTCATCTCTTCATGGAAATAAGTACTTAAACAATAAAACTAAACTAATTT GAGATTTCCTGTTAACAAAGCAGTAATGGTACCTTGCTAGATAAAACTGTTAAAATGGTAGAGT ATCTTTGTTTCTTCCCAAGAGGTGAGTTCTCTTATTACACTCACCAGACATCCGGGGGAGGAAA ATCAAGAGTTAGTAGGACAAGCTAAAGGTGGGCAGTAAGATGACTTCCAGATCTGGGAAGAATC TCTATTAGTTTTTTTTACATGTAGATACAATTATCTACTTCAAACATTTAATTTACTCCTAAAG CTAGTTTTTTGTGCTGTATTTGTTATCAAGATAGAGGATCTTAATAATGTCTCGTGTTTGCTGT TCTACTTTTTGAAGAAATGGATGCCTGCTGTTGAGGAACAATGGGTGGTAATGACCACTAACCT CAAGCCACAAATGGAATTGGTGAGGACCAAAGGATTTGAGATCTATGAGACATCTAAAAGCGCC GTAACTCCACATATTGTCAAGGTTCAGGAGCTAGCAAAACCTCACTTTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.53043962

Conservation Information
Conserved circRNAs	NA
PMCS	0.53043962

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							58407140-58408232(+)
58408198-58408217(-)
						
						Potential amino acid sequence
						
							MNTMIQKAVEKKAQAEVWAAPHIETVKTKWMPAVEEQWVVMTTNLKPQMELVRTKGFEIYETSK
SAVTPHIVKVQELAKPHFQSLLEKHWEEHGKPAMNTMIQKAVEKKAQAEVWAAPHIETVKTKWM
PAVEEQWVVMTTNLKPQMELVRTKGFEIYETSKSAVTPHIVKVQELAKPHFQSLLEKHWEEHGK
PAMNTMIQKAVEKKAQAEVWAAPHIETVKTKWMPAVEEQWVVMTTNLKPQMELVRTKGFEIYET
SKSAVTPHIVKVQELAKPHFQSLLEKHWEEHGKPAMNTMIQKAVEKKAQAEVWAAPHIETVKTK
WMPAVEEQWVVMTTNLKPQMELVRTKGFEIYETSKSAVTPHIVKVQELAKPHFQ(+)
MWSYGAFRCLIDLKSFGPHQFHLWLEVSGHYHPLFLNSRHPFCFDCFYMWRSPYLSLSLLLNCL
LYHCVHSRFSVLLPMLLQKRLKVRFC*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to low-temperature stress

Functional Information
Coding potential	Y
Potential coding position	58407140-58408232(+) 58408198-58408217(-)
Potential amino acid sequence	MNTMIQKAVEKKAQAEVWAAPHIETVKTKWMPAVEEQWVVMTTNLKPQMELVRTKGFEIYETSK SAVTPHIVKVQELAKPHFQSLLEKHWEEHGKPAMNTMIQKAVEKKAQAEVWAAPHIETVKTKWM PAVEEQWVVMTTNLKPQMELVRTKGFEIYETSKSAVTPHIVKVQELAKPHFQSLLEKHWEEHGK PAMNTMIQKAVEKKAQAEVWAAPHIETVKTKWMPAVEEQWVVMTTNLKPQMELVRTKGFEIYET SKSAVTPHIVKVQELAKPHFQSLLEKHWEEHGKPAMNTMIQKAVEKKAQAEVWAAPHIETVKTK WMPAVEEQWVVMTTNLKPQMELVRTKGFEIYETSKSAVTPHIVKVQELAKPHFQ(+) MWSYGAFRCLIDLKSFGPHQFHLWLEVSGHYHPLFLNSRHPFCFDCFYMWRSPYLSLSLLLNCL LYHCVHSRFSVLLPMLLQKRLKVRFC*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to low-temperature stress

			
						 Other Information
					
						References
						Zuo et al., 2016; Yin et al., 2018;Yang et al., 2020

circRNA

Detail information of Solyc08g074290.2_circ_g.1

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