Plant Circular RNA Database

Detail information of orai.008G000600_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.008G000600_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000802
					
						Alias
						Chr08:132488|138493
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr08: 132488-138493  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.008G000600						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.008G000600.1:7
Gorai.008G000600.2:7
Gorai.008G000600.3:7

General Information
CircRNA Name	orai.008G000600_circ_g.1
ID in PlantcircBase	gra_circ_000802
Alias	Chr08:132488\|138493
Organism	Gossypium raimondii
Position	chrChr08: 132488-138493 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.008G000600
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	2
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.008G000600.1:7 Gorai.008G000600.2:7 Gorai.008G000600.3:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATACATCCCATTTAAGAGCTTTCAAGCGTTTACTTGGGGACGTGACTGATGGCGTCAACTCCTC
GGGCATCATTTCAACTAGAGATGCCTGTGAACCTATcttttcgactgccaatgttgagttagga
tcatggcacttccccatgctgtaagacccacactttcctgtaggatttccgaagctagcaaatt
tcacatta

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 1185
Transcript exons: 132488-132812,133410-133621,133761-133871,134632-134739,
135810-135919,137449-137558,138285-138493
CTTTTCGACTGCCAATGTTGAGTTAGGATCATGGCACTTCCCCATGCTGTAAGACCCACACTTT
CCTGTAGGATTTCCGAAGCTAGCAAATTTCACATTAGAAATCAAAGTATTTTTTGGGCACTTCA
GATGGGCAGCCGATTTTCCACTTCCACCTTTACGTACTGGCGCCTGGTCTACCATAGGAAAGTC
CTCAGCAATGAGAGAACATAGGCCCGATATTTTGCGTTTCGAGAAAGTAATTTTTGTTGGATCT
CCACCTTTCTCCTCAAAGATAACCAAAATGTTTCCAGATGGCTTGAACCAAGAACGGGGAACAT
GGTACCATCTTTGTGTTGGTTCTCCACAACCAGTAAGGCATTTGTCTGGGAAGAATTTTCCTCT
GTAATCACACTCCTCTACACACTTTTCATGTTCAGAACTTTTTATTGGCCAGTATCTTCCAATC
TCTTCTCCATTTAACCATGCTAGACCTTTTCCCATATGAATCATATCCAGACCAACTGGTTCAT
TCCCTGATGGAGAATCCACAACAACCTTGTACCATGTCAAAGGCTGATTTTTTGGTGGTTCTGA
GGTTGACACCCAATTTATGCTATTCAAACCGTCTGGTTTGTATATACTCAAGTGTTCTCCTTGC
AATCCAATCTTGTAGGTCCATCTAGACATAGATAAATCCATTGTTCCATTGTTGAGCCCCTCGA
TCTTGACACTTGTTAGTCCTGCTCCTACCCATTCATATAACCCTCCTGCATTCTGTAGGCCCAC
TGTCATGCTTAACAGTGCAATTTCATTCTTTCCCGCCTTGAGAGAAATTGGACTCTCAAATTTG
AAGGGAGAATGTGAGCCATTTCCAGAAGCACTACCTTGGCGTTCTTGATTCACAAAAGCATGAA
GAGCATGCCCCTTTGACTCAATAAGGAGGAGTGGATGGCTTGCCTTCTTCAGAAATTCTTCATT
TTCACCAACAATTATACTGGTTGTATACCAGAGGTAGTCAGTAGTATCTTTCGTGGTATTTATA
TGATCCACAAAATCATTTTTAGCAAAGTCAGCTACTCCCCAGATTCCAGCATTCTCCACAAATA
CATCCCATTTAAGAGCTTTCAAGCGTTTACTTGGGGACGTGACTGATGGCGTCAACTCCTCGGG
CATCATTTCAACTAGAGATGCCTGTGAACCTAT
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	ATACATCCCATTTAAGAGCTTTCAAGCGTTTACTTGGGGACGTGACTGATGGCGTCAACTCCTC GGGCATCATTTCAACTAGAGATGCCTGTGAACCTATcttttcgactgccaatgttgagttagga tcatggcacttccccatgctgtaagacccacactttcctgtaggatttccgaagctagcaaatt tcacatta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1185 Transcript exons: 132488-132812,133410-133621,133761-133871,134632-134739, 135810-135919,137449-137558,138285-138493 CTTTTCGACTGCCAATGTTGAGTTAGGATCATGGCACTTCCCCATGCTGTAAGACCCACACTTT CCTGTAGGATTTCCGAAGCTAGCAAATTTCACATTAGAAATCAAAGTATTTTTTGGGCACTTCA GATGGGCAGCCGATTTTCCACTTCCACCTTTACGTACTGGCGCCTGGTCTACCATAGGAAAGTC CTCAGCAATGAGAGAACATAGGCCCGATATTTTGCGTTTCGAGAAAGTAATTTTTGTTGGATCT CCACCTTTCTCCTCAAAGATAACCAAAATGTTTCCAGATGGCTTGAACCAAGAACGGGGAACAT GGTACCATCTTTGTGTTGGTTCTCCACAACCAGTAAGGCATTTGTCTGGGAAGAATTTTCCTCT GTAATCACACTCCTCTACACACTTTTCATGTTCAGAACTTTTTATTGGCCAGTATCTTCCAATC TCTTCTCCATTTAACCATGCTAGACCTTTTCCCATATGAATCATATCCAGACCAACTGGTTCAT TCCCTGATGGAGAATCCACAACAACCTTGTACCATGTCAAAGGCTGATTTTTTGGTGGTTCTGA GGTTGACACCCAATTTATGCTATTCAAACCGTCTGGTTTGTATATACTCAAGTGTTCTCCTTGC AATCCAATCTTGTAGGTCCATCTAGACATAGATAAATCCATTGTTCCATTGTTGAGCCCCTCGA TCTTGACACTTGTTAGTCCTGCTCCTACCCATTCATATAACCCTCCTGCATTCTGTAGGCCCAC TGTCATGCTTAACAGTGCAATTTCATTCTTTCCCGCCTTGAGAGAAATTGGACTCTCAAATTTG AAGGGAGAATGTGAGCCATTTCCAGAAGCACTACCTTGGCGTTCTTGATTCACAAAAGCATGAA GAGCATGCCCCTTTGACTCAATAAGGAGGAGTGGATGGCTTGCCTTCTTCAGAAATTCTTCATT TTCACCAACAATTATACTGGTTGTATACCAGAGGTAGTCAGTAGTATCTTTCGTGGTATTTATA TGATCCACAAAATCATTTTTAGCAAAGTCAGCTACTCCCCAGATTCCAGCATTCTCCACAAATA CATCCCATTTAAGAGCTTTCAAGCGTTTACTTGGGGACGTGACTGATGGCGTCAACTCCTCGGG CATCATTTCAACTAGAGATGCCTGTGAACCTAT
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.429574412

Conservation Information
Conserved circRNAs	NA
PMCS	0.429574412

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							138467-132490(-)
138442-132510(+)
						
					

						Potential amino acid sequence
						
							MMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDFVDHINTTKDTTDYLWYTTSIIV
GENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSHSPFKFESPISLKAGKNEIALLS
MTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYKIGLQGEHLSIYKPDGLNSINWV
STSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLNGEEIGRYWPIKSSEHEKCVEEC
DYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEEKGGDPTKITFSKRKISGLCSLI
AEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGNPTGKCGSYSMGKCHDPNSTLAV
EKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDFVDHINTTKDTT
DYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSHSPFKFESPISL
KAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYKIGLQGEHLSIY
KPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLNGEEIGRYWPIK
SSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEEKGGDPTKITFS
KRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGNPTGKCGSYSMG
KCHDPNSTLAVEKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDF
VDHINTTKDTTDYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSH
SPFKFESPISLKAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYK
IGLQGEHLSIYKPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLN
GEEIGRYWPIKSSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEE
KGGDPTKITFSKRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGN
PTGKCGSYSMGKCHDPNSTLAVEKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGI
WGVADFAKNDFVDHINTTKDTTDYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQE
RQGSASGNGSHSPFKFESPISLKAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGT
MDLSMSRWTYKIGLQGEHLSIYKPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDM
IHMGKGLAWLNGEEIGRYWPIKSSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFK
PSGNILVIFEEKGGDPTKITFSKRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTL
ISNVKFASFGNPTGKCGSYSMGKCHDPNSTLAVEK(-)
MASTPRASFQLEMPVNLSFRLPMLS*(+)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	138467-132490(-) 138442-132510(+)
Potential amino acid sequence	MMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDFVDHINTTKDTTDYLWYTTSIIV GENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSHSPFKFESPISLKAGKNEIALLS MTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYKIGLQGEHLSIYKPDGLNSINWV STSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLNGEEIGRYWPIKSSEHEKCVEEC DYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEEKGGDPTKITFSKRKISGLCSLI AEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGNPTGKCGSYSMGKCHDPNSTLAV EKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDFVDHINTTKDTT DYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSHSPFKFESPISL KAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYKIGLQGEHLSIY KPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLNGEEIGRYWPIK SSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEEKGGDPTKITFS KRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGNPTGKCGSYSMG KCHDPNSTLAVEKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGIWGVADFAKNDF VDHINTTKDTTDYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQERQGSASGNGSH SPFKFESPISLKAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGTMDLSMSRWTYK IGLQGEHLSIYKPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDMIHMGKGLAWLN GEEIGRYWPIKSSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFKPSGNILVIFEE KGGDPTKITFSKRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTLISNVKFASFGN PTGKCGSYSMGKCHDPNSTLAVEKIGSQASLVEMMPEELTPSVTSPSKRLKALKWDVFVENAGI WGVADFAKNDFVDHINTTKDTTDYLWYTTSIIVGENEEFLKKASHPLLLIESKGHALHAFVNQE RQGSASGNGSHSPFKFESPISLKAGKNEIALLSMTVGLQNAGGLYEWVGAGLTSVKIEGLNNGT MDLSMSRWTYKIGLQGEHLSIYKPDGLNSINWVSTSEPPKNQPLTWYKVVVDSPSGNEPVGLDM IHMGKGLAWLNGEEIGRYWPIKSSEHEKCVEECDYRGKFFPDKCLTGCGEPTQRWYHVPRSWFK PSGNILVIFEEKGGDPTKITFSKRKISGLCSLIAEDFPMVDQAPVRKGGSGKSAAHLKCPKNTL ISNVKFASFGNPTGKCGSYSMGKCHDPNSTLAVEK(-) MASTPRASFQLEMPVNLSFRLPMLS*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.008G000600_circ_g.1

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