Plant Circular RNA Database

Detail information of Os06g0281400_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os06g0281400_circ_g.3
					
						ID in PlantcircBase
						osa_circ_030548
					
						Alias
						Os_ciR2215
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 9844886-9850447  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
					
						Parent gene
						
							Os06g0281400						
					
						Parent gene annotation
						
							Similar to DNA topoisomerase. (Os06t0281400-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0281400_circ_g.1 Os06g0281400_circ_g.2 Os06g0281400_circ_g.4 						
					
						Support reads
						6/2
					
						Tissues
						root/shoot, root, pistil
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0281400-01:8

General Information
CircRNA Name	Os06g0281400_circ_g.3
ID in PlantcircBase	osa_circ_030548
Alias	Os_ciR2215
Organism	Oryza sativa
Position	chr6: 9844886-9850447 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
Parent gene	Os06g0281400
Parent gene annotation	Similar to DNA topoisomerase. (Os06t0281400-01)
Parent gene strand	-
Alternative splicing	Os06g0281400_circ_g.1 Os06g0281400_circ_g.2 Os06g0281400_circ_g.4
Support reads	6/2
Tissues	root/shoot, root, pistil
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0281400-01:8

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCTTATGCTGGTCGGCCTTATGGCTTCATGCGCTTCTTGAGCATTTTTCACCTTCTTGAAGTAT
TTTCTAATGCCCTCTGAAGCATATTCCTCACCATACctcagtctccatgttagcagtaaagctg
agatctgcaacttcagagaaatgatgcataagaaacgccgagacctgaggaagatggtaccgaa
catttgtc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 673
Transcript exons: 9844886-9844957,9846135-9846194,9846991-9847059,9847188-
9847271,9847395-9847478,9848289-9848381,9849113-9849213,
9850338-9850447
CTCAGTCTCCATGTTAGCAGTAAAGCTGAGATCTGCAACTTCAGAGAAATGATGCATAAGAAAC
GCCGAGACCATCCGACCACGAAATTCAGGGTGTAGAACCCGACTTTTTATTGTCACATATTTAC
GATCCTGCAATACTTTCATTATAGATGCATATGTGGATGGCCTTCCAATTCCAAGTTCTTCTAG
CTTTTTAATCAATGCACTCTCAGAATAACGTGATGGAGGTTTAGTAAAGTGCTGTGAAAGATGA
ACGTTAACTGGAGACACCAAGTCCTTAACCTCTAATTTGGACAAGGCCTCAAAGTTATCCTGGT
GAACAGCATCAACCTCAGAATTGTAACTGGAAGGACTTGCTTCAGTGTCATCATATACAGCTTG
GTATCCCTTGAAGTCAAGCCTTGATGCAGATGAATGAAAAATCATGTCTCCTTCAGAATTACCA
ATATCAACTTGAATCATATCAGTTCTAGAAGCTTCCATCTGGCAAGCCATAGTTCTTTTCCATA
TTAGAGTATACAGTTTCAAAGAATCATCATCAAGTGCTCCGACCAAAGATGATGGCAACCTCCT
TATGCTGGTCGGCCTTATGGCTTCATGCGCTTCTTGAGCATTTTTCACCTTCTTGAAGTATTTT
CTAATGCCCTCTGAAGCATATTCCTCACCATAC
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CCTTATGCTGGTCGGCCTTATGGCTTCATGCGCTTCTTGAGCATTTTTCACCTTCTTGAAGTAT TTTCTAATGCCCTCTGAAGCATATTCCTCACCATACctcagtctccatgttagcagtaaagctg agatctgcaacttcagagaaatgatgcataagaaacgccgagacctgaggaagatggtaccgaa catttgtc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 673 Transcript exons: 9844886-9844957,9846135-9846194,9846991-9847059,9847188- 9847271,9847395-9847478,9848289-9848381,9849113-9849213, 9850338-9850447 CTCAGTCTCCATGTTAGCAGTAAAGCTGAGATCTGCAACTTCAGAGAAATGATGCATAAGAAAC GCCGAGACCATCCGACCACGAAATTCAGGGTGTAGAACCCGACTTTTTATTGTCACATATTTAC GATCCTGCAATACTTTCATTATAGATGCATATGTGGATGGCCTTCCAATTCCAAGTTCTTCTAG CTTTTTAATCAATGCACTCTCAGAATAACGTGATGGAGGTTTAGTAAAGTGCTGTGAAAGATGA ACGTTAACTGGAGACACCAAGTCCTTAACCTCTAATTTGGACAAGGCCTCAAAGTTATCCTGGT GAACAGCATCAACCTCAGAATTGTAACTGGAAGGACTTGCTTCAGTGTCATCATATACAGCTTG GTATCCCTTGAAGTCAAGCCTTGATGCAGATGAATGAAAAATCATGTCTCCTTCAGAATTACCA ATATCAACTTGAATCATATCAGTTCTAGAAGCTTCCATCTGGCAAGCCATAGTTCTTTTCCATA TTAGAGTATACAGTTTCAAAGAATCATCATCAAGTGCTCCGACCAAAGATGATGGCAACCTCCT TATGCTGGTCGGCCTTATGGCTTCATGCGCTTCTTGAGCATTTTTCACCTTCTTGAAGTATTTT CTAATGCCCTCTGAAGCATATTCCTCACCATAC
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006660* 						
					
						PMCS
						0.312056669

Conservation Information
Conserved circRNAs	osi_circ_006660*
PMCS	0.312056669

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9850419-9846158(+)
9849150-9850441(-)
						
						Potential amino acid sequence
						
							MPSEAYSSPYLSLHVSSKAEICNFREMMHKKRRDHPTTKFRV*(+)
MACQMEASRTDMIQVDIGNSEGDMIFHSSASRLDFKGYQAVYDDTEASPSSYNSEVDAVHQDNF
EALSKLEVKDLVSPVNVHLSQHFTKPPSRYSESALIKKLEELGIGRPSTYASIMKVLQDRKYVT
IKSRVLHPEFRGRMVSAFLMHHFSEVADLSFTANMETEVW*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9850419-9846158(+) 9849150-9850441(-)
Potential amino acid sequence	MPSEAYSSPYLSLHVSSKAEICNFREMMHKKRRDHPTTKFRV(+) MACQMEASRTDMIQVDIGNSEGDMIFHSSASRLDFKGYQAVYDDTEASPSSYNSEVDAVHQDNF EALSKLEVKDLVSPVNVHLSQHFTKPPSRYSESALIKKLEELGIGRPSTYASIMKVLQDRKYVT IKSRVLHPEFRGRMVSAFLMHHFSEVADLSFTANMETEVW(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0281400_circ_g.3

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