Plant Circular RNA Database

Detail information of orai.009G249000_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.009G249000_circ_g.2
					
						ID in PlantcircBase
						gra_circ_000974
					
						Alias
						Chr09:20136010|20137929
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr09: 20136010-20137929  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.009G249000						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.009G249000_circ_g.1 						
					
						Support reads
						40/112
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.009G249000.2:8
Gorai.009G249000.5:6
Gorai.009G249000.4:8
Gorai.009G249000.1:7
Gorai.009G249000.3:7

General Information
CircRNA Name	orai.009G249000_circ_g.2
ID in PlantcircBase	gra_circ_000974
Alias	Chr09:20136010\|20137929
Organism	Gossypium raimondii
Position	chrChr09: 20136010-20137929 JBrowse»
Reference genome	Graimondii_221
Type	ue-circRNA
Identification method	CIRI
Parent gene	Gorai.009G249000
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.009G249000_circ_g.1
Support reads	40/112
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.009G249000.2:8 Gorai.009G249000.5:6 Gorai.009G249000.4:8 Gorai.009G249000.1:7 Gorai.009G249000.3:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTGCACAAACGAAAATTGTCCTTATAGACACGTGCATGTGAATCCAAATGCCTCGACTTGCGAA
GGATTTCTTAGGGGCTATTGTGCTGACGGTAATGAGttgtagcaaagacccataaaccttccaa
attctctctggtctggactccacatagtgcaaggttatcaaataatgatgggagttcactatgt
tacccgaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1052
Transcript exons: 20136010-20136208,20136293-20136443,20136572-20136689,20
136805-20136879,20137180-20137270,20137355-20137625,2013
7711-20137753,20137826-20137929
TTGTAGCAAAGACCCATAAACCTTCCAAATTCTCTCTGGTCTGGACTCCACATAGTGCAAGGTT
ATCAAATAATGATGGGAGTTCACTATGTTACCCGAAGGTCCGCCCGCAACTGTTTCCCTGGAAA
AGAATGGCACACAAGAGAAGCTTTAAGTTGAACTCAGTTTCTTCCTACAGTAGTTCTTTATCTA
CAATTGGAAGGAAAATGCTGCTATTGAGAAAGAGAAATACTGTTTATACACGGTCAATTAATGG
ATTTTCAATTCACAAATCAAAGGTGTTAAGTGTTGGTGGTTCTAGTTTGAAATGGTCAAAATCT
ATTGAAAGGCATTCAAGGAAAGCTAATGAGGAAGCTACTCTAGCAGTTGCTGAAGCTGAAAGAA
AGAAAAGAGAACAAAGCACTGTTTCTAGGACAGGCAAAAAAGGTCTCTCTTGCCACAAGGTTCA
TGGTACAGAAGTTCGGCGAGGTGAACGAATATTCCGCATTGGTTCAGTGCGCTACAAAATGGAT
TCTTCTAGGCGTTCCCTTCAGAGGATATCAGATGATGCGTCGTCATGCTCTGCTAGTCAGCAAT
CAGAAAACAGTACCAAAAAGTCTTATGTCCCAAGGAGACTAGTCATCGGGAATGATGAGTATGT
CCGAATTGGCAATGGCAACCAGCTTGTTAGAGATCCAAAGAAGCGTACTCGTGTTTTGGCAAGT
GAGAAAGTTCGTTGGAGTTTGCACACTGCAAGGTTACGGTTGGTTAAAAAGCGCAAGTACTGTC
AATTTTTCACAAGGTTTGGGAAATGCAACAAAGATGATGGAAAGTGTCCCTATATTCACGATCC
CTCCAAAATATCTGTATGCACAAAATTTCTCAAGGGTCTATGTTCAAATCCCAACTGCAAATTG
ACTCATAAGGTCATTCCAGAACGAATGCCTGATTGTTCATATTTCCTGCAAGGTTTGTGCACAA
ACGAAAATTGTCCTTATAGACACGTGCATGTGAATCCAAATGCCTCGACTTGCGAAGGATTTCT
TAGGGGCTATTGTGCTGACGGTAATGAG
						
					

						Genomic sequence
						
							TTGTAGCAAAGACCCATAAACCTTCCAAATTCTCTCTGGTCTGGACTCCACATAGTGCAAGGTT
ATCAAATAATGATGGGAGTTCACTATGTTACCCGAAGGTCCGCCCGCAACTGTTTCCCTGGAAA
AGAATGGCACACAAGAGAAGCTTTAAGTTGAACTCAGTTTCTTCCTACAGTAGTTCTTTATCTA
CAATTGGGTATAACTCTTGTTTTAGGCTTTTAGTAGCTTCTTGTTTTTATCCTGTTGTCCAAGA
AAATAAAAGAATACATTTCTTTTGCAGAAGGAAAATGCTGCTATTGAGAAAGAGAAATACTGTT
TATACACGGTCAATTAATGGATTTTCAATTCACAAATCAAAGGTGTTAAGTGTTGGTGGTTCTA
GTTTGAAATGGTCAAAATCTATTGAAAGGCATTCAAGGAAAGCTAATGAGGTAAGGTGCATCAT
GTACTGGTGGACCGAGTTCTTACATCTTTCGTATACACTTCAAAACTGAAGTGATTGACTTCTG
AATTAAAATGGCTTTTTTTGTTTGTAAAATTTTATGTTAATGTTTAATAGGAAGCTACTCTAGC
AGTTGCTGAAGCTGAAAGAAAGAAAAGAGAACAAAGCACTGTTTCTAGGACAGGCAAAAAAGGT
CTCTCTTGCCACAAGGTTCATGGTACAGAAGTTCGGCGAGGTAGGCAAATTAACAATACTTAGC
GAATTAATGGCACTTTATCTAATGTGCATGATGCACGATTTTGAAAAGAATGCTTCAATGGTAT
GTTTCATGTAAATATTTGATACTTTAGGTGAACGAATATTCCGCATTGGTTCAGTGCGCTACAA
AATGGATTCTTCTAGGCGTTCCCTTCAGAGGATATCAGGTTTCTCTTAATTTATCTCATAGTGG
ATTTAAGGTCCTTTTTGTTTTCTTTTTCTTTTAAAAACTCCCAACATTGTGTGCCGATGCAAAC
CTACTAGAGATTGAAGTTCTCATGAGGCCTGTGACATTTTGCGTTTTTTTTTAAAGAAAATTGG
TTACAGTAACAAAAAACATCTGTACTAAATAACTTGCTTCTTTCAGTTTCTGATCAGCACCTAC
TGTCTGCACTGAAGCGGATGTCTTTTTTCCCTTTTGATAAGTTTTTGTGCTGCTCTCTTAACTC
TACCTTATTTCTGTTTAGATGATGCGTCGTCATGCTCTGCTAGTCAGCAATCAGAAAACAGTAC
CAAAAAGTCTTATGTCCCAAGGAGACTAGTCATCGGGAATGATGAGTATGTTAACATTACCATA
TAAAACATAAACTTGATGGAATTACGTACAATATATAAAATTTAAATGAGCTCTGACATCTGCA
GGTATGTCCGAATTGGCAATGGCAACCAGCTTGTTAGAGATCCAAAGAAGCGTACTCGTGTTTT
GGCAAGTGAGAAAGTTCGTTGGAGTTTGCACACTGCAAGGTTACGGTTGGTTAAAAAGCGCAAG
TACTGTCAATTTTTCACAAGGTTTGGGAAATGCAACAAAGATGATGGAAAGTGTCCCTATATTC
ACGATCCCTCCAAAATATCTGTATGCACAAAATTTCTCAAGGGTCTATGTTCAAATCCCAACTG
CAAATTGACTCATAAGGTCCATCACTTGTCTGGTCAATTTGACATTCTGCTGGAGGTTGACTCC
TTGGTTAACATCTGACCTTTGATTGAATATGTTACAGGTCATTCCAGAACGAATGCCTGATTGT
TCATATTTCCTGCAAGGTGTTTCCTTTCTCAATTATGCTTTTCTTTTTCTAATTTTAGATATGC
AGATTCTGACAGAATTACTGGCAGGTTTGTGCACAAACGAAAATTGTCCTTATAGACACGTGCA
TGTGAATCCAAATGCCTCGACTTGCGAAGGATTTCTTAGGGGCTATTGTGCTGACGGTAATGAG
						
					

			
		

Sequences
Splice junction sequence	GTGCACAAACGAAAATTGTCCTTATAGACACGTGCATGTGAATCCAAATGCCTCGACTTGCGAA GGATTTCTTAGGGGCTATTGTGCTGACGGTAATGAGttgtagcaaagacccataaaccttccaa attctctctggtctggactccacatagtgcaaggttatcaaataatgatgggagttcactatgt tacccgaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1052 Transcript exons: 20136010-20136208,20136293-20136443,20136572-20136689,20 136805-20136879,20137180-20137270,20137355-20137625,2013 7711-20137753,20137826-20137929 TTGTAGCAAAGACCCATAAACCTTCCAAATTCTCTCTGGTCTGGACTCCACATAGTGCAAGGTT ATCAAATAATGATGGGAGTTCACTATGTTACCCGAAGGTCCGCCCGCAACTGTTTCCCTGGAAA AGAATGGCACACAAGAGAAGCTTTAAGTTGAACTCAGTTTCTTCCTACAGTAGTTCTTTATCTA CAATTGGAAGGAAAATGCTGCTATTGAGAAAGAGAAATACTGTTTATACACGGTCAATTAATGG ATTTTCAATTCACAAATCAAAGGTGTTAAGTGTTGGTGGTTCTAGTTTGAAATGGTCAAAATCT ATTGAAAGGCATTCAAGGAAAGCTAATGAGGAAGCTACTCTAGCAGTTGCTGAAGCTGAAAGAA AGAAAAGAGAACAAAGCACTGTTTCTAGGACAGGCAAAAAAGGTCTCTCTTGCCACAAGGTTCA TGGTACAGAAGTTCGGCGAGGTGAACGAATATTCCGCATTGGTTCAGTGCGCTACAAAATGGAT TCTTCTAGGCGTTCCCTTCAGAGGATATCAGATGATGCGTCGTCATGCTCTGCTAGTCAGCAAT CAGAAAACAGTACCAAAAAGTCTTATGTCCCAAGGAGACTAGTCATCGGGAATGATGAGTATGT CCGAATTGGCAATGGCAACCAGCTTGTTAGAGATCCAAAGAAGCGTACTCGTGTTTTGGCAAGT GAGAAAGTTCGTTGGAGTTTGCACACTGCAAGGTTACGGTTGGTTAAAAAGCGCAAGTACTGTC AATTTTTCACAAGGTTTGGGAAATGCAACAAAGATGATGGAAAGTGTCCCTATATTCACGATCC CTCCAAAATATCTGTATGCACAAAATTTCTCAAGGGTCTATGTTCAAATCCCAACTGCAAATTG ACTCATAAGGTCATTCCAGAACGAATGCCTGATTGTTCATATTTCCTGCAAGGTTTGTGCACAA ACGAAAATTGTCCTTATAGACACGTGCATGTGAATCCAAATGCCTCGACTTGCGAAGGATTTCT TAGGGGCTATTGTGCTGACGGTAATGAG
Genomic sequence	TTGTAGCAAAGACCCATAAACCTTCCAAATTCTCTCTGGTCTGGACTCCACATAGTGCAAGGTT ATCAAATAATGATGGGAGTTCACTATGTTACCCGAAGGTCCGCCCGCAACTGTTTCCCTGGAAA AGAATGGCACACAAGAGAAGCTTTAAGTTGAACTCAGTTTCTTCCTACAGTAGTTCTTTATCTA CAATTGGGTATAACTCTTGTTTTAGGCTTTTAGTAGCTTCTTGTTTTTATCCTGTTGTCCAAGA AAATAAAAGAATACATTTCTTTTGCAGAAGGAAAATGCTGCTATTGAGAAAGAGAAATACTGTT TATACACGGTCAATTAATGGATTTTCAATTCACAAATCAAAGGTGTTAAGTGTTGGTGGTTCTA GTTTGAAATGGTCAAAATCTATTGAAAGGCATTCAAGGAAAGCTAATGAGGTAAGGTGCATCAT GTACTGGTGGACCGAGTTCTTACATCTTTCGTATACACTTCAAAACTGAAGTGATTGACTTCTG AATTAAAATGGCTTTTTTTGTTTGTAAAATTTTATGTTAATGTTTAATAGGAAGCTACTCTAGC AGTTGCTGAAGCTGAAAGAAAGAAAAGAGAACAAAGCACTGTTTCTAGGACAGGCAAAAAAGGT CTCTCTTGCCACAAGGTTCATGGTACAGAAGTTCGGCGAGGTAGGCAAATTAACAATACTTAGC GAATTAATGGCACTTTATCTAATGTGCATGATGCACGATTTTGAAAAGAATGCTTCAATGGTAT GTTTCATGTAAATATTTGATACTTTAGGTGAACGAATATTCCGCATTGGTTCAGTGCGCTACAA AATGGATTCTTCTAGGCGTTCCCTTCAGAGGATATCAGGTTTCTCTTAATTTATCTCATAGTGG ATTTAAGGTCCTTTTTGTTTTCTTTTTCTTTTAAAAACTCCCAACATTGTGTGCCGATGCAAAC CTACTAGAGATTGAAGTTCTCATGAGGCCTGTGACATTTTGCGTTTTTTTTTAAAGAAAATTGG TTACAGTAACAAAAAACATCTGTACTAAATAACTTGCTTCTTTCAGTTTCTGATCAGCACCTAC TGTCTGCACTGAAGCGGATGTCTTTTTTCCCTTTTGATAAGTTTTTGTGCTGCTCTCTTAACTC TACCTTATTTCTGTTTAGATGATGCGTCGTCATGCTCTGCTAGTCAGCAATCAGAAAACAGTAC CAAAAAGTCTTATGTCCCAAGGAGACTAGTCATCGGGAATGATGAGTATGTTAACATTACCATA TAAAACATAAACTTGATGGAATTACGTACAATATATAAAATTTAAATGAGCTCTGACATCTGCA GGTATGTCCGAATTGGCAATGGCAACCAGCTTGTTAGAGATCCAAAGAAGCGTACTCGTGTTTT GGCAAGTGAGAAAGTTCGTTGGAGTTTGCACACTGCAAGGTTACGGTTGGTTAAAAAGCGCAAG TACTGTCAATTTTTCACAAGGTTTGGGAAATGCAACAAAGATGATGGAAAGTGTCCCTATATTC ACGATCCCTCCAAAATATCTGTATGCACAAAATTTCTCAAGGGTCTATGTTCAAATCCCAACTG CAAATTGACTCATAAGGTCCATCACTTGTCTGGTCAATTTGACATTCTGCTGGAGGTTGACTCC TTGGTTAACATCTGACCTTTGATTGAATATGTTACAGGTCATTCCAGAACGAATGCCTGATTGT TCATATTTCCTGCAAGGTGTTTCCTTTCTCAATTATGCTTTTCTTTTTCTAATTTTAGATATGC AGATTCTGACAGAATTACTGGCAGGTTTGTGCACAAACGAAAATTGTCCTTATAGACACGTGCA TGTGAATCCAAATGCCTCGACTTGCGAAGGATTTCTTAGGGGCTATTGTGCTGACGGTAATGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							gar_circ_000813* 						
					
						PMCS
						1.137468429

Conservation Information
Conserved circRNAs	gar_circ_000813*
PMCS	1.137468429

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20137702-20136012(+)
20137877-20136025(+)
20136063-20137910(-)
						
						Potential amino acid sequence
						
							MLQVIPERMPDCSYFLQGLCTNENCPYRHVHVNPNASTCEGFLRGYCADGNEL*(+)
MPRLAKDFLGAIVLTVMSCSKDP*(+)
MWSPDQREFGRFMGLCYNSLPSAQ*(-)
						
						Sponge-miRNAs
						
							gra-miR8740 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	20137702-20136012(+) 20137877-20136025(+) 20136063-20137910(-)
Potential amino acid sequence	MLQVIPERMPDCSYFLQGLCTNENCPYRHVHVNPNASTCEGFLRGYCADGNEL(+) MPRLAKDFLGAIVLTVMSCSKDP(+) MWSPDQREFGRFMGLCYNSLPSAQ*(-)
Sponge-miRNAs	gra-miR8740
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.009G249000_circ_g.2

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