Plant Circular RNA Database

Detail information of AT4G20340_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT4G20340_circ_g.4
					
						ID in PlantcircBase
						ath_circ_031849
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 10985725-10986348  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						KNIFE
					
						Parent gene
						
							AT4G20340						
					
						Parent gene annotation
						
							Transcription factor TFIIE, alpha subunit
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G20340_circ_g.1 AT4G20340_circ_g.2 AT4G20340_circ_g.3 AT4G20340_circ_g.5 						
					
						Support reads
						1
					
						Tissues
						inflorescences
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G20340.2:3
AT4G20340.1:3
AT4G20340.3:3

General Information
CircRNA Name	AT4G20340_circ_g.4
ID in PlantcircBase	ath_circ_031849
Alias	NA
Organism	Arabidpsis thaliana
Position	chr4: 10985725-10986348 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	KNIFE
Parent gene	AT4G20340
Parent gene annotation	Transcription factor TFIIE, alpha subunit
Parent gene strand	+
Alternative splicing	AT4G20340_circ_g.1 AT4G20340_circ_g.2 AT4G20340_circ_g.3 AT4G20340_circ_g.5
Support reads	1
Tissues	inflorescences
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G20340.2:3 AT4G20340.1:3 AT4G20340.3:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGAATGTAACAAGCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCAAGGAGACGGCAG
CGGGAGAAAGTAAAAGTTTGGCTTCAGGATTTGGAGactgcaaagcgtgcgaagatgtatagtt
atgcagtgggtggtacaactgatggccgagcagaggacaatgttaagttccacacgcactcgta
ttgctgtc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 408
Transcript exons: 10985725-10985838,10985963-10986072,10986165-10986348
ACTGCAAAGCGTGCGAAGATGTATAGTTATGCAGTGGGTGGTACAACTGATGGCCGAGCAGAGG
ACAATGTTAAGTTCCACACGCACTCGTATTGCTGTCTTGATTATGCACAGATATATGATATTGT
TCGTTATAAACTGCACCGGCTCAAGAAAAAGTTCAAAGATGAACTAGAAGACAGAAATACTGTT
CAGGAGTATGGCTGTCCTAACTGCAAAAGAAAATATAATGCACTGGATGCCCTGAGATTAATTT
CTATGGAAGACGATTCTTTTCATTGTGAAAATTGCAATGGTGAACTTGTTATGGAATGTAACAA
GCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCAAGGAGACGGCAGCGGGAGAAAGTA
AAAGTTTGGCTTCAGGATTTGGAG
						
						Genomic sequence
						
							ACTGCAAAGCGTGCGAAGATGTATAGTTATGCAGTGGGTGGTACAACTGATGGCCGAGCAGAGG
ACAATGTTAAGTTCCACACGCACTCGTATTGCTGTCTTGATTATGCACAGGTATGCTTCATGTC
CCCTAATATTTATTTTTTTCTTTCAGTTTGCTGTTATGCTTTTTGTTTTTTCTTTCTGGTTTCT
TTTTTGTTTAATCAAACTACCCTTTATCACTTCTTCTCTCTTACAGATATATGATATTGTTCGT
TATAAACTGCACCGGCTCAAGAAAAAGTTCAAAGATGAACTAGAAGACAGAAATACTGTTCAGG
AGTATGGCTGTCCTAACTGCAAAAGAAAGTAAAGTCCACTATTCTTTATGTTTTTAAATCTTTA
CATGGTGAGTTGTATTATTTTCACTAAACCTGTATAGATTCTCATTTCTGGTATAGATATAATG
CACTGGATGCCCTGAGATTAATTTCTATGGAAGACGATTCTTTTCATTGTGAAAATTGCAATGG
TGAACTTGTTATGGAATGTAACAAGCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCA
AGGAGACGGCAGCGGGAGAAAGTAAAAGTTTGGCTTCAGGATTTGGAG

Sequences
Splice junction sequence	GGAATGTAACAAGCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCAAGGAGACGGCAG CGGGAGAAAGTAAAAGTTTGGCTTCAGGATTTGGAGactgcaaagcgtgcgaagatgtatagtt atgcagtgggtggtacaactgatggccgagcagaggacaatgttaagttccacacgcactcgta ttgctgtc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 408 Transcript exons: 10985725-10985838,10985963-10986072,10986165-10986348 ACTGCAAAGCGTGCGAAGATGTATAGTTATGCAGTGGGTGGTACAACTGATGGCCGAGCAGAGG ACAATGTTAAGTTCCACACGCACTCGTATTGCTGTCTTGATTATGCACAGATATATGATATTGT TCGTTATAAACTGCACCGGCTCAAGAAAAAGTTCAAAGATGAACTAGAAGACAGAAATACTGTT CAGGAGTATGGCTGTCCTAACTGCAAAAGAAAATATAATGCACTGGATGCCCTGAGATTAATTT CTATGGAAGACGATTCTTTTCATTGTGAAAATTGCAATGGTGAACTTGTTATGGAATGTAACAA GCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCAAGGAGACGGCAGCGGGAGAAAGTA AAAGTTTGGCTTCAGGATTTGGAG
Genomic sequence	ACTGCAAAGCGTGCGAAGATGTATAGTTATGCAGTGGGTGGTACAACTGATGGCCGAGCAGAGG ACAATGTTAAGTTCCACACGCACTCGTATTGCTGTCTTGATTATGCACAGGTATGCTTCATGTC CCCTAATATTTATTTTTTTCTTTCAGTTTGCTGTTATGCTTTTTGTTTTTTCTTTCTGGTTTCT TTTTTGTTTAATCAAACTACCCTTTATCACTTCTTCTCTCTTACAGATATATGATATTGTTCGT TATAAACTGCACCGGCTCAAGAAAAAGTTCAAAGATGAACTAGAAGACAGAAATACTGTTCAGG AGTATGGCTGTCCTAACTGCAAAAGAAAGTAAAGTCCACTATTCTTTATGTTTTTAAATCTTTA CATGGTGAGTTGTATTATTTTCACTAAACCTGTATAGATTCTCATTTCTGGTATAGATATAATG CACTGGATGCCCTGAGATTAATTTCTATGGAAGACGATTCTTTTCATTGTGAAAATTGCAATGG TGAACTTGTTATGGAATGTAACAAGCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCA AGGAGACGGCAGCGGGAGAAAGTAAAAGTTTGGCTTCAGGATTTGGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.217016309

Conservation Information
Conserved circRNAs	NA
PMCS	0.217016309

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10985743-10986345(+)
						
						Potential amino acid sequence
						
							MYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCP
NCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQREKVKVWLQD
LETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRN
TVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQRE
KVKVWLQDLETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKF
KDELEDRNTVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGD
NARRRQREKVKVWLQDLETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYK
LHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLIS
EEVVDRGDNARRRQREKVKVWLQDLE(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	10985743-10986345(+)
Potential amino acid sequence	MYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCP NCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQREKVKVWLQD LETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRN TVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQRE KVKVWLQDLETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKF KDELEDRNTVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLISEEVVDRGD NARRRQREKVKVWLQDLETAKRAKMYSYAVGGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYK LHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISMEDDSFHCENCNGELVMECNKLIS EEVVDRGDNARRRQREKVKVWLQDLE(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT4G20340_circ_g.4

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