Plant Circular RNA Database

Detail information of AT5G26830_circ_g.19

			
						 General Information
					
						CircRNA Name
						AT5G26830_circ_g.19
					
						ID in PlantcircBase
						ath_circ_040672
					
						Alias
						Ath_circ_FC3536
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 9440627-9441382  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT5G26830						
					
						Parent gene annotation
						
							Threonine--tRNA ligase, mitochondrial 1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G26830_circ_g.4 AT5G26830_circ_g.5 AT5G26830_circ_g.6 AT5G26830_circ_g.7 AT5G26830_circ_g.8 AT5G26830_circ_g.9 AT5G26830_circ_g.10 AT5G26830_circ_g.11 AT5G26830_circ_g.12 AT5G26830_circ_g.13 AT5G26830_circ_g.14 AT5G26830_circ_g.15 AT5G26830_circ_g.16 AT5G26830_circ_g.17 AT5G26830_circ_g.18 AT5G26830_circ_g.20 						
					
						Support reads
						2
					
						Tissues
						root, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G26830.1:4

General Information
CircRNA Name	AT5G26830_circ_g.19
ID in PlantcircBase	ath_circ_040672
Alias	Ath_circ_FC3536
Organism	Arabidpsis thaliana
Position	chr5: 9440627-9441382 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	AT5G26830
Parent gene annotation	Threonine--tRNA ligase, mitochondrial 1
Parent gene strand	+
Alternative splicing	AT5G26830_circ_g.4 AT5G26830_circ_g.5 AT5G26830_circ_g.6 AT5G26830_circ_g.7 AT5G26830_circ_g.8 AT5G26830_circ_g.9 AT5G26830_circ_g.10 AT5G26830_circ_g.11 AT5G26830_circ_g.12 AT5G26830_circ_g.13 AT5G26830_circ_g.14 AT5G26830_circ_g.15 AT5G26830_circ_g.16 AT5G26830_circ_g.17 AT5G26830_circ_g.18 AT5G26830_circ_g.20
Support reads	2
Tissues	root, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G26830.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGTGATGATGATTGTGGTGTGTTTGTAGGTGCGAGAAGCTCAGCTTGCTCAGTACAACTACATT
CTGGTCGTGGGTGAAACAGAAGCTGCTACAGGACAGttgaacgaaggagatggtgcattttatg
gtccaaagatagacatcacagtttctgatgcaatgaataggaaattccagtgtgctactttaca
ggttatac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 459
Transcript exons: 9440627-9440719,9440820-9441041,9441143-9441214,9441311-
9441382
TTGAACGAAGGAGATGGTGCATTTTATGGTCCAAAGATAGACATCACAGTTTCTGATGCAATGA
ATAGGAAATTCCAGTGTGCTACTTTACAGCTTGATTTTCAACTTCCGATTCGCTTCAACTTGGA
ATACGCAGCTGAGGATGAAGCGAAAAAGAGTAGACCTGTAATGATACATAGAGCCGTGTTGGGA
TCTGTGGAACGTATGTTTGCCATATTGTTGGAGCATTACAAAGGAAAATGGCCCTTCTGGATTA
GTCCGCGACAGGCCATAGTTTGTCCTATATCAGAGAAATCTCAGCAATATGCAGAAAAGGTTCA
AAAGCAAATTAAAGATGCTGGGTTCTATGTTGATGCTGACTTAACAGACAGAAAGATAGATAAA
AAGGTGCGAGAAGCTCAGCTTGCTCAGTACAACTACATTCTGGTCGTGGGTGAAACAGAAGCTG
CTACAGGACAG
						
						Genomic sequence
						
							TTGAACGAAGGAGATGGTGCATTTTATGGTCCAAAGATAGACATCACAGTTTCTGATGCAATGA
ATAGGAAATTCCAGTGTGCTACTTTACAGGTTATACTCCCAAATTCTTTTTAGTTTTTCTTGTC
TATGCATTTCTCTTGCTGTTATCAGTGCAGGAGTTGATTTTATGTTTCTTGTGGCCTCAAATCA
GCTTGATTTTCAACTTCCGATTCGCTTCAACTTGGAATACGCAGCTGAGGATGAAGCGAAAAAG
AGTAGACCTGTAATGATACATAGAGCCGTGTTGGGATCTGTGGAACGTATGTTTGCCATATTGT
TGGAGCATTACAAAGGAAAATGGCCCTTCTGGATTAGTCCGCGACAGGCCATAGTTTGTCCTAT
ATCAGAGAAATCTCAGCAATATGCAGAAAAGGTTACTTTTGTGGCTGTTGCTGTACTCTTTTAC
TGAGCACATGTGGTTCTTATCGACTTATTTGCAGTGTCTAATCTCTGGTTTTTCTTTTGACACA
ACAGGTTCAAAAGCAAATTAAAGATGCTGGGTTCTATGTTGATGCTGACTTAACAGACAGAAAG
ATAGATAAAAAGGTAAACGATTTCGGTTCATGTTCCAAGTTTTGTTCTGTGGTTGGCTGGTGAT
TCCTAAGATTAACTTGAGTGATGATGATTGTGGTGTGTTTGTAGGTGCGAGAAGCTCAGCTTGC
TCAGTACAACTACATTCTGGTCGTGGGTGAAACAGAAGCTGCTACAGGACAG

Sequences
Splice junction sequence	AGTGATGATGATTGTGGTGTGTTTGTAGGTGCGAGAAGCTCAGCTTGCTCAGTACAACTACATT CTGGTCGTGGGTGAAACAGAAGCTGCTACAGGACAGttgaacgaaggagatggtgcattttatg gtccaaagatagacatcacagtttctgatgcaatgaataggaaattccagtgtgctactttaca ggttatac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 459 Transcript exons: 9440627-9440719,9440820-9441041,9441143-9441214,9441311- 9441382 TTGAACGAAGGAGATGGTGCATTTTATGGTCCAAAGATAGACATCACAGTTTCTGATGCAATGA ATAGGAAATTCCAGTGTGCTACTTTACAGCTTGATTTTCAACTTCCGATTCGCTTCAACTTGGA ATACGCAGCTGAGGATGAAGCGAAAAAGAGTAGACCTGTAATGATACATAGAGCCGTGTTGGGA TCTGTGGAACGTATGTTTGCCATATTGTTGGAGCATTACAAAGGAAAATGGCCCTTCTGGATTA GTCCGCGACAGGCCATAGTTTGTCCTATATCAGAGAAATCTCAGCAATATGCAGAAAAGGTTCA AAAGCAAATTAAAGATGCTGGGTTCTATGTTGATGCTGACTTAACAGACAGAAAGATAGATAAA AAGGTGCGAGAAGCTCAGCTTGCTCAGTACAACTACATTCTGGTCGTGGGTGAAACAGAAGCTG CTACAGGACAG
Genomic sequence	TTGAACGAAGGAGATGGTGCATTTTATGGTCCAAAGATAGACATCACAGTTTCTGATGCAATGA ATAGGAAATTCCAGTGTGCTACTTTACAGGTTATACTCCCAAATTCTTTTTAGTTTTTCTTGTC TATGCATTTCTCTTGCTGTTATCAGTGCAGGAGTTGATTTTATGTTTCTTGTGGCCTCAAATCA GCTTGATTTTCAACTTCCGATTCGCTTCAACTTGGAATACGCAGCTGAGGATGAAGCGAAAAAG AGTAGACCTGTAATGATACATAGAGCCGTGTTGGGATCTGTGGAACGTATGTTTGCCATATTGT TGGAGCATTACAAAGGAAAATGGCCCTTCTGGATTAGTCCGCGACAGGCCATAGTTTGTCCTAT ATCAGAGAAATCTCAGCAATATGCAGAAAAGGTTACTTTTGTGGCTGTTGCTGTACTCTTTTAC TGAGCACATGTGGTTCTTATCGACTTATTTGCAGTGTCTAATCTCTGGTTTTTCTTTTGACACA ACAGGTTCAAAAGCAAATTAAAGATGCTGGGTTCTATGTTGATGCTGACTTAACAGACAGAAAG ATAGATAAAAAGGTAAACGATTTCGGTTCATGTTCCAAGTTTTGTTCTGTGGTTGGCTGGTGAT TCCTAAGATTAACTTGAGTGATGATGATTGTGGTGTGTTTGTAGGTGCGAGAAGCTCAGCTTGC TCAGTACAACTACATTCTGGTCGTGGGTGAAACAGAAGCTGCTACAGGACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.289242781

Conservation Information
Conserved circRNAs	NA
PMCS	0.289242781

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9440687-9441379(+)
						
						Potential amino acid sequence
						
							MNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFW
ISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETE
AATGQLNEGDGAFYGPKIDITVSDAMNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIH
RAVLGSVERMFAILLEHYKGKWPFWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTD
RKIDKKVREAQLAQYNYILVVGETEAATGQLNEGDGAFYGPKIDITVSDAMNRKFQCATLQLDF
QLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIVCPISEK
SQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATGQLNEGDGAFY
GPKIDITVSDAMNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAIL
LEHYKGKWPFWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQ
YNYILVVGETEAATGQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9440687-9441379(+)
Potential amino acid sequence	MNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFW ISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETE AATGQLNEGDGAFYGPKIDITVSDAMNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIH RAVLGSVERMFAILLEHYKGKWPFWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTD RKIDKKVREAQLAQYNYILVVGETEAATGQLNEGDGAFYGPKIDITVSDAMNRKFQCATLQLDF QLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIVCPISEK SQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATGQLNEGDGAFY GPKIDITVSDAMNRKFQCATLQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAIL LEHYKGKWPFWISPRQAIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQ YNYILVVGETEAATGQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G26830_circ_g.19

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