Plant Circular RNA Database

Detail information of Pg_S3138.5_circ_igg.1

			
						 General Information
					
						CircRNA Name
						Pg_S3138.5_circ_igg.1
					
						ID in PlantcircBase
						pgi_circ_000181
					
						Alias
						novel_circ_0000605
					
						Organism
						
							Panax ginseng						
					
						Position
						
							chrPg_scaffold3138: 42107-42448  JBrowse»						
					
						Reference genome
						
							ginseng_v1						
					
						Type  
						
							igg-circRNA							
									
						Identification method
						find_circ, CIRI
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						root
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-TA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	Pg_S3138.5_circ_igg.1
ID in PlantcircBase	pgi_circ_000181
Alias	novel_circ_0000605
Organism	Panax ginseng
Position	chrPg_scaffold3138: 42107-42448 JBrowse»
Reference genome	ginseng_v1
Type	igg-circRNA
Identification method	find_circ, CIRI
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	NA
Support reads	NA
Tissues	root
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-TA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GACATGCGCTTTGGACCTAC
 R: CTTTCTAGCACCTCTCTCCGA
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GACATGCGCTTTGGACCTAC R: CTTTCTAGCACCTCTCTCCGA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TAAAGAAGGACTTCACTACTGTTTATGACATGCGCTTTGGACCTACTTCAGGCTTTGGTTGGAA
TGATAAGACTCATATTGTCACTGCCCATATCTCAAGgttatggagccaagtattcaagattcgg
agagaggtgctagaaagaataagagaaagtggagtaaagtggaagatgaaaagttaattgaagc
tttacttg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTTATGGAGCCAAGTATTCAAGATTCGGAGAGAGGTGCTAGAAAGAATAAGAGAAAGTGGAGTA
AAGTGGAAGATGAAAAGTTAATTGAAGCTTTACTTGAGTTGGTGAATGCTGGAACCCACAAGGC
GGACAATAGATTTAGGCCTGGATACTTGGTTGTTCTTGAAGATGCATTGCTTACTAAGCTTCAA
GGATCAGATCTTTAGGGCAAGGCCTCATATAGAGTCTCGAATTAAGACTCTAAAGAAGGACTTC
ACTACTGTTTATGACATGCGCTTTGGACCTACTTCAGGCTTTGGTTGGAATGATAAGACTCATA
TTGTCACTGCCCATATCTCAAG

Sequences
Splice junction sequence	TAAAGAAGGACTTCACTACTGTTTATGACATGCGCTTTGGACCTACTTCAGGCTTTGGTTGGAA TGATAAGACTCATATTGTCACTGCCCATATCTCAAGgttatggagccaagtattcaagattcgg agagaggtgctagaaagaataagagaaagtggagtaaagtggaagatgaaaagttaattgaagc tttacttg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTTATGGAGCCAAGTATTCAAGATTCGGAGAGAGGTGCTAGAAAGAATAAGAGAAAGTGGAGTA AAGTGGAAGATGAAAAGTTAATTGAAGCTTTACTTGAGTTGGTGAATGCTGGAACCCACAAGGC GGACAATAGATTTAGGCCTGGATACTTGGTTGTTCTTGAAGATGCATTGCTTACTAAGCTTCAA GGATCAGATCTTTAGGGCAAGGCCTCATATAGAGTCTCGAATTAAGACTCTAAAGAAGGACTTC ACTACTGTTTATGACATGCGCTTTGGACCTACTTCAGGCTTTGGTTGGAATGATAAGACTCATA TTGTCACTGCCCATATCTCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS

Conservation Information
Conserved circRNAs	NA
PMCS

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Bian et al., 2021

circRNA

Detail information of Pg_S3138.5_circ_igg.1

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