Plant Circular RNA Database

Detail information of GLYMA_08G2649001_circ_g.3

			
						 General Information
					
						CircRNA Name
						GLYMA_08G2649001_circ_g.3
					
						ID in PlantcircBase
						gma_circ_008589
					
						Alias
						gma_circ_0000279
					
						Organism
						
							Glycine max						
					
						Position
						
							chr8: 30766306-30767498  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							GLYMA_08G2649001						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							GLYMA_08G2649001_circ_g.1 GLYMA_08G2649001_circ_igg.1 GLYMA_08G2649001_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							TG-CT							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRH45327:3
KRH45328:3
KRH45326:3
KRH45325:3
KRH45323:3
KRH45324:3

General Information
CircRNA Name	GLYMA_08G2649001_circ_g.3
ID in PlantcircBase	gma_circ_008589
Alias	gma_circ_0000279
Organism	Glycine max
Position	chr8: 30766306-30767498 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	CIRI
Parent gene	GLYMA_08G2649001
Parent gene annotation	hypothetical protein
Parent gene strand	-
Alternative splicing	GLYMA_08G2649001_circ_g.1 GLYMA_08G2649001_circ_igg.1 GLYMA_08G2649001_circ_g.2
Support reads	NA
Tissues	leaf
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	TG-CT The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRH45327:3 KRH45328:3 KRH45326:3 KRH45325:3 KRH45323:3 KRH45324:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CCAGATAACCCAATAAATGCAGGT
 R: CCTTTTGACGAGCATCATAACGA
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CCAGATAACCCAATAAATGCAGGT R: CCTTTTGACGAGCATCATAACGA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAATGACACAAGTCAATATACATAATTTAGTGATCAAAACAAGGTAAGAAAGGTAGGACTGACC
CAATGAATTTGATAGTATTGCCATGGTCATCTTTGAtcaggcatggtagcagtatcaccattcc
tatcattaataatggattttgggtccttaacagtttggacaacctcagtgatggaacccaaagc
tttctcct

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TCAGGCATGGTAGCAGTATCACCATTCCTATCATTAATAATGGATTTTGGGTCCTTAACAGTTT
GGACAACCTCAGTGATGGAACCCAAAGCTTTCTCCTTTTGACGAGCTGGTAAATGAAATTAAAT
GATTATATGTAAATGCAGAATCTCACACACTACTAAAAATGGCTCAACATATTACCATCATAAC
GAATTAATGATTTAGGTAATCCATTTGGTCGTAATGCCTTTTCATTCATGCCTTCTGTGTACTT
GCTCACCTCAACCTGCATTCTGTAGTAATAACTGTATTAAGCATTAAGCAAATTTAATCTGTTA
CAACCTTTCTCCATGAAAGCACATTTCTAGCTTTGTTAATCTTAAGAGTTTGTGTAAGGATCAT
TACTGTTATTTATACATTCCTATCAGTTTGATTGGATGCATGACTTACTTATTATTTTCTTCTT
TTAAAAAAAAATATCAAATGTACTAAGCCTCACTCCAGATAATTTAAACCATTTGAAACAACAA
TTAATAGATATAAGATTAAACTAACTTCCTTTATAGTCCAAAAACAAGCCACAGTCATGATATG
TCTAGCAGCAAAAGGATTTTATGTTTTGAAAGTAAAGGAAAAGGAAATGATGAATAAGCATTGT
TAAAGATCAAATGTGTGTTTGGTTTGTCATTTGAAATGTCCCATACCATGTTTTGGACAAAAGA
TTGTCTCTTGTAGTTTACGCCTAAACCATGGTTCTGGAATTTGAGACAGGAAACCTAACACCTT
ATAACTTTACTGAATGCTTCAACAGTTATGGAGCACCCAAGAATGATTTAACATCTTTAGCACA
CCCCCTCGCACAAGTGTCTTGTTGGTTTGAAGCATAAATAATGAACAGAGGCCTTGTTCTAAAA
TTTTACTTAATTATAAAAAAAAGGAGGGCTCTAGGATATCACTCCTAATCACTTTATCTTCGAC
TCTCTAATACCTTGTCAAAGACACATTTTCTTAAAACTTTGAGCTTTCAAGTAAAGTTACAAGA
ATGGTTTTATATTTCTATTTCTAACGTGTTTTGCCAATTGGATTTCCATTATCATGTGAACTTA
AGTAAAAATGACACAAGTCAATATACATAATTTAGTGATCAAAACAAGGTAAGAAAGGTAGGAC
TGACCCAATGAATTTGATAGTATTGCCATGGTCATCTTTGA

Sequences
Splice junction sequence	AAATGACACAAGTCAATATACATAATTTAGTGATCAAAACAAGGTAAGAAAGGTAGGACTGACC CAATGAATTTGATAGTATTGCCATGGTCATCTTTGAtcaggcatggtagcagtatcaccattcc tatcattaataatggattttgggtccttaacagtttggacaacctcagtgatggaacccaaagc tttctcct
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TCAGGCATGGTAGCAGTATCACCATTCCTATCATTAATAATGGATTTTGGGTCCTTAACAGTTT GGACAACCTCAGTGATGGAACCCAAAGCTTTCTCCTTTTGACGAGCTGGTAAATGAAATTAAAT GATTATATGTAAATGCAGAATCTCACACACTACTAAAAATGGCTCAACATATTACCATCATAAC GAATTAATGATTTAGGTAATCCATTTGGTCGTAATGCCTTTTCATTCATGCCTTCTGTGTACTT GCTCACCTCAACCTGCATTCTGTAGTAATAACTGTATTAAGCATTAAGCAAATTTAATCTGTTA CAACCTTTCTCCATGAAAGCACATTTCTAGCTTTGTTAATCTTAAGAGTTTGTGTAAGGATCAT TACTGTTATTTATACATTCCTATCAGTTTGATTGGATGCATGACTTACTTATTATTTTCTTCTT TTAAAAAAAAATATCAAATGTACTAAGCCTCACTCCAGATAATTTAAACCATTTGAAACAACAA TTAATAGATATAAGATTAAACTAACTTCCTTTATAGTCCAAAAACAAGCCACAGTCATGATATG TCTAGCAGCAAAAGGATTTTATGTTTTGAAAGTAAAGGAAAAGGAAATGATGAATAAGCATTGT TAAAGATCAAATGTGTGTTTGGTTTGTCATTTGAAATGTCCCATACCATGTTTTGGACAAAAGA TTGTCTCTTGTAGTTTACGCCTAAACCATGGTTCTGGAATTTGAGACAGGAAACCTAACACCTT ATAACTTTACTGAATGCTTCAACAGTTATGGAGCACCCAAGAATGATTTAACATCTTTAGCACA CCCCCTCGCACAAGTGTCTTGTTGGTTTGAAGCATAAATAATGAACAGAGGCCTTGTTCTAAAA TTTTACTTAATTATAAAAAAAAGGAGGGCTCTAGGATATCACTCCTAATCACTTTATCTTCGAC TCTCTAATACCTTGTCAAAGACACATTTTCTTAAAACTTTGAGCTTTCAAGTAAAGTTACAAGA ATGGTTTTATATTTCTATTTCTAACGTGTTTTGCCAATTGGATTTCCATTATCATGTGAACTTA AGTAAAAATGACACAAGTCAATATACATAATTTAGTGATCAAAACAAGGTAAGAAAGGTAGGAC TGACCCAATGAATTTGATAGTATTGCCATGGTCATCTTTGA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.041357809

Conservation Information
Conserved circRNAs	NA
PMCS	0.041357809

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						playing a key role in low-temperature-responsive regulation

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	playing a key role in low-temperature-responsive regulation

			
						 Other Information
					
						References
						Wang et al., 2020

circRNA

Detail information of GLYMA_08G2649001_circ_g.3

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