Plant Circular RNA Database

Detail information of AT2G21520_circ_g.8

			
						 General Information
					
						CircRNA Name
						AT2G21520_circ_g.8
					
						ID in PlantcircBase
						ath_circ_014239
					
						Alias
						At_ciR3532
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 9217198-9217826  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						PcircRNA_finder, find_circ
					
						Parent gene
						
							AT2G21520						
					
						Parent gene annotation
						
							Sec14p-like phosphatidylinositol transfer family protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G21520_circ_g.1 AT2G21520_circ_g.2 AT2G21520_circ_g.3 AT2G21520_circ_g.4 AT2G21520_circ_g.5 AT2G21520_circ_g.6 AT2G21520_circ_g.7 AT2G21520_circ_g.9 AT2G21520_circ_g.10 						
					
						Support reads
						2/1
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G21520.2:4
AT2G21520.3:4
AT2G21520.1:4

General Information
CircRNA Name	AT2G21520_circ_g.8
ID in PlantcircBase	ath_circ_014239
Alias	At_ciR3532
Organism	Arabidpsis thaliana
Position	chr2: 9217198-9217826 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	PcircRNA_finder, find_circ
Parent gene	AT2G21520
Parent gene annotation	Sec14p-like phosphatidylinositol transfer family protein
Parent gene strand	+
Alternative splicing	AT2G21520_circ_g.1 AT2G21520_circ_g.2 AT2G21520_circ_g.3 AT2G21520_circ_g.4 AT2G21520_circ_g.5 AT2G21520_circ_g.6 AT2G21520_circ_g.7 AT2G21520_circ_g.9 AT2G21520_circ_g.10
Support reads	2/1
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G21520.2:4 AT2G21520.3:4 AT2G21520.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GATGGTGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAGTGTTGAACAGCGAAGGG
AAAGTCATTGCTTATGCAAAACCTTCATATACATGGactctccaccaaatgtttattatcaatg
cagggcctggatttaggctactctggaacactgtcaagagctttcttgaccctaagacatctgc
taaaattc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 351
Transcript exons: 9217198-9217299,9217388-9217437,9217538-9217637,9217728-
9217826
ACTCTCCACCAAATGTTTATTATCAATGCAGGGCCTGGATTTAGGCTACTCTGGAACACTGTCA
AGAGCTTTCTTGACCCTAAGACATCTGCTAAAATTCATGTACTCGGATACAAGTATCTAAGCAA
ATTGCTTGAAGTCATTGATGTCAACGAGTTGCCTGAGTTCTTGGGAGGTGCATGTACTTGCGCA
GATCAAGGCGGTTGCATGCTTTCTGACAAGGGACCTTGGAAAAACCCGGAAATTGTGAAGATGG
TGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAGTGTTGAACAGCGAAGGGAAAGT
CATTGCTTATGCAAAACCTTCATATACATGG
						
						Genomic sequence
						
							ACTCTCCACCAAATGTTTATTATCAATGCAGGGCCTGGATTTAGGCTACTCTGGAACACTGTCA
AGAGCTTTCTTGACCCTAAGACATCTGCTAAAATTCATGTATGAGAGATCTAAGTTATATCTCT
ATTTAAAAGTCTTGTCTTTGATTATATAGAATTCAATATGGACTATTCTATTGTTTATGTAGGT
ACTCGGATACAAGTATCTAAGCAAATTGCTTGAAGTCATTGATGTCAAGTATGTGAACCTTGAC
CCACCCTTATTTGATCTGTTCTATCGAAAGCTGCACGTTTTTTTGTTTTTGCTTATGTTCAAAA
TGGTCTGCACTTTTTTATAGCGAGTTGCCTGAGTTCTTGGGAGGTGCATGTACTTGCGCAGATC
AAGGCGGTTGCATGCTTTCTGACAAGGGACCTTGGAAAAACCCGGAAATTGTGAAGGTCATTTA
CTTAGACTAATCTCTTTTTGGATTCTATTTGTATCATCCAAGTTGGAATCTGAGACATTTGGTT
TTCTTCTCATTATTTCAGATGGTGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAG
TGTTGAACAGCGAAGGGAAAGTCATTGCTTATGCAAAACCTTCATATACATGG

Sequences
Splice junction sequence	GATGGTGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAGTGTTGAACAGCGAAGGG AAAGTCATTGCTTATGCAAAACCTTCATATACATGGactctccaccaaatgtttattatcaatg cagggcctggatttaggctactctggaacactgtcaagagctttcttgaccctaagacatctgc taaaattc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 351 Transcript exons: 9217198-9217299,9217388-9217437,9217538-9217637,9217728- 9217826 ACTCTCCACCAAATGTTTATTATCAATGCAGGGCCTGGATTTAGGCTACTCTGGAACACTGTCA AGAGCTTTCTTGACCCTAAGACATCTGCTAAAATTCATGTACTCGGATACAAGTATCTAAGCAA ATTGCTTGAAGTCATTGATGTCAACGAGTTGCCTGAGTTCTTGGGAGGTGCATGTACTTGCGCA GATCAAGGCGGTTGCATGCTTTCTGACAAGGGACCTTGGAAAAACCCGGAAATTGTGAAGATGG TGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAGTGTTGAACAGCGAAGGGAAAGT CATTGCTTATGCAAAACCTTCATATACATGG
Genomic sequence	ACTCTCCACCAAATGTTTATTATCAATGCAGGGCCTGGATTTAGGCTACTCTGGAACACTGTCA AGAGCTTTCTTGACCCTAAGACATCTGCTAAAATTCATGTATGAGAGATCTAAGTTATATCTCT ATTTAAAAGTCTTGTCTTTGATTATATAGAATTCAATATGGACTATTCTATTGTTTATGTAGGT ACTCGGATACAAGTATCTAAGCAAATTGCTTGAAGTCATTGATGTCAAGTATGTGAACCTTGAC CCACCCTTATTTGATCTGTTCTATCGAAAGCTGCACGTTTTTTTGTTTTTGCTTATGTTCAAAA TGGTCTGCACTTTTTTATAGCGAGTTGCCTGAGTTCTTGGGAGGTGCATGTACTTGCGCAGATC AAGGCGGTTGCATGCTTTCTGACAAGGGACCTTGGAAAAACCCGGAAATTGTGAAGGTCATTTA CTTAGACTAATCTCTTTTTGGATTCTATTTGTATCATCCAAGTTGGAATCTGAGACATTTGGTT TTCTTCTCATTATTTCAGATGGTGCTTCATGGAGGAGCACATCGAGCGAGGCAGGTGGTGAAAG TGTTGAACAGCGAAGGGAAAGTCATTGCTTATGCAAAACCTTCATATACATGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.28835315

Conservation Information
Conserved circRNAs	NA
PMCS	0.28835315

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9217210-9217823(+)
						
						Potential amino acid sequence
						
							MFIINAGPGFRLLWNTVKSFLDPKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGG
CMLSDKGPWKNPEIVKMVLHGGAHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFR
LLWNTVKSFLDPKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKN
PEIVKMVLHGGAHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFRLLWNTVKSFLD
PKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMVLHGG
AHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFRLLWNTVKSFLDPKTSAKIHVLG
YKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMVLHGGAHRARQVVKVL
NSEGKVIAYAKPSYTW(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9217210-9217823(+)
Potential amino acid sequence	MFIINAGPGFRLLWNTVKSFLDPKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGG CMLSDKGPWKNPEIVKMVLHGGAHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFR LLWNTVKSFLDPKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKN PEIVKMVLHGGAHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFRLLWNTVKSFLD PKTSAKIHVLGYKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMVLHGG AHRARQVVKVLNSEGKVIAYAKPSYTWTLHQMFIINAGPGFRLLWNTVKSFLDPKTSAKIHVLG YKYLSKLLEVIDVNELPEFLGGACTCADQGGCMLSDKGPWKNPEIVKMVLHGGAHRARQVVKVL NSEGKVIAYAKPSYTW(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of AT2G21520_circ_g.8

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