Plant Circular RNA Database

Detail information of Os02g0556600_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os02g0556600_circ_g.4
					
						ID in PlantcircBase
						osa_circ_015027
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 21046290-21048405  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0556600						
					
						Parent gene annotation
						
							Similar to Pherophorin-dz1 protein precursor. (Os02t0556600-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0556600_circ_g.1 Os02g0556600_circ_g.2 Os02g0556600_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0556600-01:5

General Information
CircRNA Name	Os02g0556600_circ_g.4
ID in PlantcircBase	osa_circ_015027
Alias	NA
Organism	Oryza sativa
Position	chr2: 21046290-21048405 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0556600
Parent gene annotation	Similar to Pherophorin-dz1 protein precursor. (Os02t0556600-01)
Parent gene strand	-
Alternative splicing	Os02g0556600_circ_g.1 Os02g0556600_circ_g.2 Os02g0556600_circ_g.3
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0556600-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTCACCAGAAGTGAATTTGGGACCAAATGACTCACTTTTCCCAGGACCACGGTAAAGATAGCCA
TCATCGCCATGATATCCACAGCTGTTAGACTCCCAGctgacgcagcaattttctgtggctaaga
ccatacatttcaggaggttccccataaccattttgatgattggatggaggatcagtttcacttg
ccatatca

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 511
Transcript exons: 21046290-21046443,21046733-21046809,21047240-21047285,21
047773-21047859,21048259-21048405
CTGACGCAGCAATTTTCTGTGGCTAAGACCATACATTTCAGGAGGTTCCCCATAACCATTTTGA
TGATTGGATGGAGGATCAGTTTCACTTGCCATATCAAAAGAATTTAATGTATCTTGGTAGCCGT
AATGTAGGAGATATGAACGAACAATCCAATGACTGATGTCTGGTTGCAAATCCAATTTGTCGCT
GACAGATTGTTGCTTCATTTTCTCTTCAAAAATATAGCCCTCAATGTCAAAACAGAATGGTTCT
TTTCCAAAGTTTACTGTCACCTCTTCATCTTGACTATGGACTGCAATGGTAGGATATAGTGGGC
CTTTAATTTCTTTTTGAAAGGATCCAACTAGAGATCCATTTTTCGTGAAGAAAAATTCTTGTGA
GAAATAGTTGATGCCAGCACCAATTGTGTCACCAGAAGTGAATTTGGGACCAAATGACTCACTT
TTCCCAGGACCACGGTAAAGATAGCCATCATCGCCATGATATCCACAGCTGTTAGACTCCCAG
						
					

						Genomic sequence
						
							CTGACGCAGCAATTTTCTGTGGCTAAGACCATACATTTCAGGAGGTTCCCCATAACCATTTTGA
TGATTGGATGGAGGATCAGTTTCACTTGCCATATCAAAAGAATTTAATGTATCTTGGTAGCCGT
AATGTAGGAGATATGAACGAACAATCCTGTTACAAGTGCACAGTACATGTACATAAGGCGGCAA
AGTATAAAAGATTTAATGTCAATATAGGTACAATTAAAGATTGAGACAAGGTAGGAATGAAGGG
CGGAGTGAATATGGTATCAAATGTAGATTACGTAATATGGTAAATTTCACATAAACATAACAGG
AGCAGACAAATAATTGACAGCACAAGTCAGTGATTATCAAACATAACTCGCTAACCTATCTCAC
AAGATACAGAATGATCTCAAGATGAAACAGTGATGGATTCTTAACATGAGAAAGCATACCAATG
ACTGATGTCTGGTTGCAAATCCAATTTGTCGCTGACAGATTGTTGCTTCATTTTCTCTTCAAAA
ATATAGCCCTGCCAATAAGAATCATACTTAGTGTTATATCATGTGTGCATGAACTAAACACCAA
ATAGGTAAATATACATGAAAAGAATGAGCTGAGTGATAATTGTAGGCTATAATTCCATAATGAA
AACCAATTGGTTCTTATGGCACCATACACCATATTTTGGTGGCCTGATTACTACCTAAAAGGAT
GCATGCAACCAAAAGTTCTTTCAATTATATGAAAGGAGCTGCGCTTAAGATGCAATGCATCTGA
ATTTGGAAAACTAAGATGGATAATCTATAAGACATTATGATATATGTTTAATTGATGCAGCAAA
CTGTGGAATGGAAAACATGGAATTTGAAATAATACTCCATAAGGAAATCATGACAATATTACAT
TTTTGTGTGAGGTCCTTAATAGGTGAATAGGAATATCTAGATCTATTGGCTCACCTCAATGTCA
AAACAGAATGGTTCTTTTCCAAAGTTTACTGTCACCCTGATTTGCAAAAATGAACAATCAGATC
CATTGAAAAGCTGAAAATTAATATAATAATTTATGAGTTATGGCACATAACGCATGCAATTCCA
AGCCTAAATAATATATTAACACAAGCAAATAATTTCACTTAACACCATTTTTCAGCAACTTAGG
TTTAACTGTCTTGGTTAAAATGCAATTTTCCATATTTGGAATGTCTATTTTCATTGAAATAATC
ACTTAGGCAGCATTCTTGCTGTTAGGTTGGAGAGAGTTTTTCAGCGCACGCAAAACGAGAAAGC
TCATTAGCATGGATTAATTAAGTATTAATTATTGTAAACTTGAAAAATGAATTCATTTGAATTT
TTTAAACAGCTTCTATATAGAATTTTTTTTTTGTAAAAGATACATCATTTGACAGTTTGAAAAG
CGTGCAAATGGAAAACCAGGAAGTTGAAGTTTGGAGTTGAAGAAAAGAACTAAGTCTTAGGAAC
GGGTATCTTACTCTTCATCTTGACTATGGACTGCAATGGTAGGATATAGTGGGCCTTTAATTTC
TTTTTGAAAGGATCCAACTAGAGATCCATTTTTCCTGCAAAATGGTACATGTAATTAGATTAAA
GTGCAGAATGATATCCTGAGATGAATAAGCATGGTCAAGGCTTCTGAAGGAGAAAAAGAAACAA
GCACATGGAAAGTTAAGCCAGTACCAGAGGCGTAGTGGCAGATGCAAATGCAACTGATTTCCAA
ATTCAATATTTAACAGCCAACAGCTGTAGTGAAGACAACTCTTGCGAATTTCAAAATATTAATG
AAAGCAGCAACAAAGCACAGCATTCATCTCAAGTCAAGAAAAAAACTCACAAGACAAAGGATGA
AAGCTATGAACTTTAAGAAAGTCTAAAATATGACGACTAAAAGGCTAATATAGAATAGCTAATC
GGAGAAGCAGACGCTGCCATGCCAATTTAGAAAGAATGACAGAACTTACGTGAAGAAAAATTCT
TGTGAGAAATAGTTGATGCCAGCACCAATTGTGTCACCAGAAGTGAATTTGGGACCAAATGACT
CACTTTTCCCAGGACCACGGTAAAGATAGCCATCATCGCCATGATATCCACAGCTGTTAGACTC
CCAG
						
					

			
		

Sequences
Splice junction sequence	GTCACCAGAAGTGAATTTGGGACCAAATGACTCACTTTTCCCAGGACCACGGTAAAGATAGCCA TCATCGCCATGATATCCACAGCTGTTAGACTCCCAGctgacgcagcaattttctgtggctaaga ccatacatttcaggaggttccccataaccattttgatgattggatggaggatcagtttcacttg ccatatca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 511 Transcript exons: 21046290-21046443,21046733-21046809,21047240-21047285,21 047773-21047859,21048259-21048405 CTGACGCAGCAATTTTCTGTGGCTAAGACCATACATTTCAGGAGGTTCCCCATAACCATTTTGA TGATTGGATGGAGGATCAGTTTCACTTGCCATATCAAAAGAATTTAATGTATCTTGGTAGCCGT AATGTAGGAGATATGAACGAACAATCCAATGACTGATGTCTGGTTGCAAATCCAATTTGTCGCT GACAGATTGTTGCTTCATTTTCTCTTCAAAAATATAGCCCTCAATGTCAAAACAGAATGGTTCT TTTCCAAAGTTTACTGTCACCTCTTCATCTTGACTATGGACTGCAATGGTAGGATATAGTGGGC CTTTAATTTCTTTTTGAAAGGATCCAACTAGAGATCCATTTTTCGTGAAGAAAAATTCTTGTGA GAAATAGTTGATGCCAGCACCAATTGTGTCACCAGAAGTGAATTTGGGACCAAATGACTCACTT TTCCCAGGACCACGGTAAAGATAGCCATCATCGCCATGATATCCACAGCTGTTAGACTCCCAG
Genomic sequence	CTGACGCAGCAATTTTCTGTGGCTAAGACCATACATTTCAGGAGGTTCCCCATAACCATTTTGA TGATTGGATGGAGGATCAGTTTCACTTGCCATATCAAAAGAATTTAATGTATCTTGGTAGCCGT AATGTAGGAGATATGAACGAACAATCCTGTTACAAGTGCACAGTACATGTACATAAGGCGGCAA AGTATAAAAGATTTAATGTCAATATAGGTACAATTAAAGATTGAGACAAGGTAGGAATGAAGGG CGGAGTGAATATGGTATCAAATGTAGATTACGTAATATGGTAAATTTCACATAAACATAACAGG AGCAGACAAATAATTGACAGCACAAGTCAGTGATTATCAAACATAACTCGCTAACCTATCTCAC AAGATACAGAATGATCTCAAGATGAAACAGTGATGGATTCTTAACATGAGAAAGCATACCAATG ACTGATGTCTGGTTGCAAATCCAATTTGTCGCTGACAGATTGTTGCTTCATTTTCTCTTCAAAA ATATAGCCCTGCCAATAAGAATCATACTTAGTGTTATATCATGTGTGCATGAACTAAACACCAA ATAGGTAAATATACATGAAAAGAATGAGCTGAGTGATAATTGTAGGCTATAATTCCATAATGAA AACCAATTGGTTCTTATGGCACCATACACCATATTTTGGTGGCCTGATTACTACCTAAAAGGAT GCATGCAACCAAAAGTTCTTTCAATTATATGAAAGGAGCTGCGCTTAAGATGCAATGCATCTGA ATTTGGAAAACTAAGATGGATAATCTATAAGACATTATGATATATGTTTAATTGATGCAGCAAA CTGTGGAATGGAAAACATGGAATTTGAAATAATACTCCATAAGGAAATCATGACAATATTACAT TTTTGTGTGAGGTCCTTAATAGGTGAATAGGAATATCTAGATCTATTGGCTCACCTCAATGTCA AAACAGAATGGTTCTTTTCCAAAGTTTACTGTCACCCTGATTTGCAAAAATGAACAATCAGATC CATTGAAAAGCTGAAAATTAATATAATAATTTATGAGTTATGGCACATAACGCATGCAATTCCA AGCCTAAATAATATATTAACACAAGCAAATAATTTCACTTAACACCATTTTTCAGCAACTTAGG TTTAACTGTCTTGGTTAAAATGCAATTTTCCATATTTGGAATGTCTATTTTCATTGAAATAATC ACTTAGGCAGCATTCTTGCTGTTAGGTTGGAGAGAGTTTTTCAGCGCACGCAAAACGAGAAAGC TCATTAGCATGGATTAATTAAGTATTAATTATTGTAAACTTGAAAAATGAATTCATTTGAATTT TTTAAACAGCTTCTATATAGAATTTTTTTTTTGTAAAAGATACATCATTTGACAGTTTGAAAAG CGTGCAAATGGAAAACCAGGAAGTTGAAGTTTGGAGTTGAAGAAAAGAACTAAGTCTTAGGAAC GGGTATCTTACTCTTCATCTTGACTATGGACTGCAATGGTAGGATATAGTGGGCCTTTAATTTC TTTTTGAAAGGATCCAACTAGAGATCCATTTTTCCTGCAAAATGGTACATGTAATTAGATTAAA GTGCAGAATGATATCCTGAGATGAATAAGCATGGTCAAGGCTTCTGAAGGAGAAAAAGAAACAA GCACATGGAAAGTTAAGCCAGTACCAGAGGCGTAGTGGCAGATGCAAATGCAACTGATTTCCAA ATTCAATATTTAACAGCCAACAGCTGTAGTGAAGACAACTCTTGCGAATTTCAAAATATTAATG AAAGCAGCAACAAAGCACAGCATTCATCTCAAGTCAAGAAAAAAACTCACAAGACAAAGGATGA AAGCTATGAACTTTAAGAAAGTCTAAAATATGACGACTAAAAGGCTAATATAGAATAGCTAATC GGAGAAGCAGACGCTGCCATGCCAATTTAGAAAGAATGACAGAACTTACGTGAAGAAAAATTCT TGTGAGAAATAGTTGATGCCAGCACCAATTGTGTCACCAGAAGTGAATTTGGGACCAAATGACT CACTTTTCCCAGGACCACGGTAAAGATAGCCATCATCGCCATGATATCCACAGCTGTTAGACTC CCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.215166478

Conservation Information
Conserved circRNAs	NA
PMCS	0.215166478

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							21046790-21048396(-)
21046350-21048396(-)
						
						Potential amino acid sequence
						
							MKQQSVSDKLDLQPDISHWIVRSYLLHYGYQDTLNSFDMASETDPPSNHQNGYGEPPEMYGLSH
RKLLRQLGV*(-)
MVMGNLLKCMVLATENCCVSWESNSCGYHGDDGYLYRGPGKSESFGPKFTSGDTIGAGINYFSQ
EFFFTKNGSLVGSFQKEIKGPLYPTIAVHSQDEEVTVNFGKEPFCFDIEGYIFEEKMKQQSVSD
KLDLQPDISHWIVRSYLLHYGYQDTLNSFDMASETDPPSNHQNGYGEPPEMYGLSHRKLLRQLG
V*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	21046790-21048396(-) 21046350-21048396(-)
Potential amino acid sequence	MKQQSVSDKLDLQPDISHWIVRSYLLHYGYQDTLNSFDMASETDPPSNHQNGYGEPPEMYGLSH RKLLRQLGV(-) MVMGNLLKCMVLATENCCVSWESNSCGYHGDDGYLYRGPGKSESFGPKFTSGDTIGAGINYFSQ EFFFTKNGSLVGSFQKEIKGPLYPTIAVHSQDEEVTVNFGKEPFCFDIEGYIFEEKMKQQSVSD KLDLQPDISHWIVRSYLLHYGYQDTLNSFDMASETDPPSNHQNGYGEPPEMYGLSHRKLLRQLG V(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0556600_circ_g.4

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