Plant Circular RNA Database

Detail information of Os03g0802300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os03g0802300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_022311
					
						Alias
						Os_ciR8928
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 33476364-33478281  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0802300						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os03t0802300-01);Conserved hypothe
tical protein. (Os03t0802300-02);Hypothetical conserved gene. (O
s03t0802300-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0802300_circ_g.2 						
					
						Support reads
						2/2
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0802300-01:3
Os03t0802300-02:3
Os03t0802300-03:2

General Information
CircRNA Name	Os03g0802300_circ_g.1
ID in PlantcircBase	osa_circ_022311
Alias	Os_ciR8928
Organism	Oryza sativa
Position	chr3: 33476364-33478281 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0802300
Parent gene annotation	Hypothetical conserved gene. (Os03t0802300-01);Conserved hypothe tical protein. (Os03t0802300-02);Hypothetical conserved gene. (O s03t0802300-03)
Parent gene strand	-
Alternative splicing	Os03g0802300_circ_g.2
Support reads	2/2
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0802300-01:3 Os03t0802300-02:3 Os03t0802300-03:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTTAAAAAAAACATTAGAGATAACATACATGGTGTTGATTGATATCTTCCTGCTCATCTTCCA
ATGAAAAATAAATATCATCTGCATGGAGCTGAAGATctgttgtgcatgaagaagagcattttga
ttttgcacttgtgcatcttgctgcatagggtagttggcccctctatttgtcgttttagtgattt
ccatcaag

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CTGTTGTGCATGAAGAAGAGCATTTTGATTTTGCACTTGTGCATCTTGCTGCATAGGGTAGTTG
GCCCCTCTATTTGTCGTTTTAGTGATTTCCATCAAGGGGCCAATGTTACTAAATGTTACAGCAG
AAAATCACTATCAGAACTTTGGCATGTAATTTTATAGAAAAAAAAATGCATTAAATACACATGC
TCTGCCATCAACATGACTCAATTTTATTACTAGCTCCTTTAGGCAAGAACTGATGTGTTAGTAT
ATATAGTAACACAGAAGGCATCTCCCTGCACCTAATACCAAAAAGAACACAGGGAAAACAAAAG
GGAAAAAAGTAAAGAGGCGCGTATATAAAATAGGTTAAAGTACCTGTAGCCAACAGTCTGTAGG
AACATCTTAAGGAGTTCAATGGATGAAAATTGCTTCCTGTAGCTGTCAGTTAGAAGCTTCAAGT
TACGCCCCAGCTTAGAGATGTGCTTAATTATTATTTCTGAAAGAACATCAATACACACTGCAGT
TCCACTTTCAAATCCTGCATTTGCCAAGTAGCGAGCAACCAACTTCCTTGTCATAAGCATTGCT
TGTTCTTGGCTTAGAGATTTTCCTTCTACTCTGGATCCATCTTTCCCTCGATATTTGTTCCCTT
TCTTATAATACTCAGGCATCATTCCAAATCTTTCAAGCATTGCAGTGTTGCGACTAACAGGCGC
CGGTAAATTATCAAGAACCCCATGAACTTCTATTTTGTAGATCCTAGACGTCTCACTTGTTCTA
GGAATTGCGCTTTCCGGGACACAGTCAGCTGGAAACCTAATTTCTGGAATGAAAGTATCATCTG
TTAAATTACTATAGTCCCCAGCATTCTTTGAGGAAGGGCCATTTTCCGACATTGGGACTGAGAT
GTTGTGACCCTGATCATCCATAAACACAGCCCTCTTCCTTTTATGCACACTGCAAACCTTCAGG
TAATTGGACATTCCCTCTGATGTTCTTTTTGGTATATCTTTGTCACCTGAACGGAACTTGCCAT
GCCATGTCCTGAATTTTTCAGCATATTGTTTGTACCATGTGTCAGGTAACTCTTCGTATCGATT
ATGTCTTTGAGATAACATAGTGTTCTCCCTGCTTGGACTAAATGAGGTTCTAGATTGCATCTGC
AGCAGCAAGGACAAATATAACCATCAGCATAGCATATGAATCACATCAACATTAGTTAGTATGC
ACCAACTGCAGCTGAATGCTATACATAATTAGTGCACATTTAATACAGTGAACTAGAAAGATTA
TCCAATGATGCATGGCATGGCCAGCAGTTACATAGCTTGACCTATCACCTATGTGAACGGTCCA
AAGAAGTTAGATTTATTTTTGGACCAATGAGTAGCATATGCTGGTCTTTAAGCAGACTACAGAA
TGACAATTAACACAAAATTGAGGTCAACAGTCAACACATCTCTTAAAAAGGAACTAATCATGTG
GTTATCATTAAAAGTATACCGTAAACCCTAACAGCTGTATGTGTGAAGTTTTCAGACGGTGAAC
AAGTCCCCGATCTAGAAAGAGAACACTAGCAATACCTAAAAGGATGCTTCACATAACAATGATT
ATACAATAGTACATAATACATAAAAGACAAAACTGCGACAATGGTGCTCAACTGGAAAGAGATA
GTGCGTGAAATCCAACTGTAACGTACAACTTGAATCTTACTTCATACATGGTAGCTGGCATTGT
TGCATAGAATAGCATATCACTCAACAGATCAAGAAAGAGAAAGGGGTTTTCTAGAAAGAGTCAC
ACAATGCTGATATTCCCAAAAAGTTTTTTTAAAAAAAACATTAGAGATAACATACATGGTGTTG
ATTGATATCTTCCTGCTCATCTTCCAATGAAAAATAAATATCATCTGCATGGAGCTGAAGAT
						
					

			
		

Sequences
Splice junction sequence	TTTTAAAAAAAACATTAGAGATAACATACATGGTGTTGATTGATATCTTCCTGCTCATCTTCCA ATGAAAAATAAATATCATCTGCATGGAGCTGAAGATctgttgtgcatgaagaagagcattttga ttttgcacttgtgcatcttgctgcatagggtagttggcccctctatttgtcgttttagtgattt ccatcaag
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGTTGTGCATGAAGAAGAGCATTTTGATTTTGCACTTGTGCATCTTGCTGCATAGGGTAGTTG GCCCCTCTATTTGTCGTTTTAGTGATTTCCATCAAGGGGCCAATGTTACTAAATGTTACAGCAG AAAATCACTATCAGAACTTTGGCATGTAATTTTATAGAAAAAAAAATGCATTAAATACACATGC TCTGCCATCAACATGACTCAATTTTATTACTAGCTCCTTTAGGCAAGAACTGATGTGTTAGTAT ATATAGTAACACAGAAGGCATCTCCCTGCACCTAATACCAAAAAGAACACAGGGAAAACAAAAG GGAAAAAAGTAAAGAGGCGCGTATATAAAATAGGTTAAAGTACCTGTAGCCAACAGTCTGTAGG AACATCTTAAGGAGTTCAATGGATGAAAATTGCTTCCTGTAGCTGTCAGTTAGAAGCTTCAAGT TACGCCCCAGCTTAGAGATGTGCTTAATTATTATTTCTGAAAGAACATCAATACACACTGCAGT TCCACTTTCAAATCCTGCATTTGCCAAGTAGCGAGCAACCAACTTCCTTGTCATAAGCATTGCT TGTTCTTGGCTTAGAGATTTTCCTTCTACTCTGGATCCATCTTTCCCTCGATATTTGTTCCCTT TCTTATAATACTCAGGCATCATTCCAAATCTTTCAAGCATTGCAGTGTTGCGACTAACAGGCGC CGGTAAATTATCAAGAACCCCATGAACTTCTATTTTGTAGATCCTAGACGTCTCACTTGTTCTA GGAATTGCGCTTTCCGGGACACAGTCAGCTGGAAACCTAATTTCTGGAATGAAAGTATCATCTG TTAAATTACTATAGTCCCCAGCATTCTTTGAGGAAGGGCCATTTTCCGACATTGGGACTGAGAT GTTGTGACCCTGATCATCCATAAACACAGCCCTCTTCCTTTTATGCACACTGCAAACCTTCAGG TAATTGGACATTCCCTCTGATGTTCTTTTTGGTATATCTTTGTCACCTGAACGGAACTTGCCAT GCCATGTCCTGAATTTTTCAGCATATTGTTTGTACCATGTGTCAGGTAACTCTTCGTATCGATT ATGTCTTTGAGATAACATAGTGTTCTCCCTGCTTGGACTAAATGAGGTTCTAGATTGCATCTGC AGCAGCAAGGACAAATATAACCATCAGCATAGCATATGAATCACATCAACATTAGTTAGTATGC ACCAACTGCAGCTGAATGCTATACATAATTAGTGCACATTTAATACAGTGAACTAGAAAGATTA TCCAATGATGCATGGCATGGCCAGCAGTTACATAGCTTGACCTATCACCTATGTGAACGGTCCA AAGAAGTTAGATTTATTTTTGGACCAATGAGTAGCATATGCTGGTCTTTAAGCAGACTACAGAA TGACAATTAACACAAAATTGAGGTCAACAGTCAACACATCTCTTAAAAAGGAACTAATCATGTG GTTATCATTAAAAGTATACCGTAAACCCTAACAGCTGTATGTGTGAAGTTTTCAGACGGTGAAC AAGTCCCCGATCTAGAAAGAGAACACTAGCAATACCTAAAAGGATGCTTCACATAACAATGATT ATACAATAGTACATAATACATAAAAGACAAAACTGCGACAATGGTGCTCAACTGGAAAGAGATA GTGCGTGAAATCCAACTGTAACGTACAACTTGAATCTTACTTCATACATGGTAGCTGGCATTGT TGCATAGAATAGCATATCACTCAACAGATCAAGAAAGAGAAAGGGGTTTTCTAGAAAGAGTCAC ACAATGCTGATATTCCCAAAAAGTTTTTTTAAAAAAAACATTAGAGATAACATACATGGTGTTG ATTGATATCTTCCTGCTCATCTTCCAATGAAAAATAAATATCATCTGCATGGAGCTGAAGAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.178486848

Conservation Information
Conserved circRNAs	NA
PMCS	0.178486848

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							33478247-33476743(+)
33476409-33478262(-)
33477513-33477321(-)
						
						Potential amino acid sequence
						
							MKNKYHLHGAEDLLCMKKSILILHLCILLHRVVGPSICRFSDFHQGANVTVANSL*(+)
MHKCKIKMLFFMHNRSSAPCR*(-)
MQSRTSFSPSRENTMLSQRHNRYEELPDTWYKQYAEKFRTWHGKFRSGDKDIPKRTSEGMSNYL
KVCSVHKRKRAVFMDDQGHNISVPMSENGPSSKNAGDYSNLTDDTFIPEIRFPADCVPESAIPR
TSETSRIYKIEVHGVLDNLPAPVSRNTAMLERFGMMPEYYKKGNKYRGKDGSRVEGKSLSQEQA
MLMTRKLVARYLANAGFESGTAVCIDVLSEIIIKHISKLGRNLKLLTDSYRKQFSSIELLKMFL
QTVGYSNIGPLMEITKTTNRGANYPMQQDAQVQNQNALLHAQQIFSSMQMIFIFHWKMSRKISI
NTICNLEPHLVQAGRTLCYLKDIIDTKSYLTHGTNNMLKNSGHGMASSVQVTKIYQKEHQRECP
IT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	33478247-33476743(+) 33476409-33478262(-) 33477513-33477321(-)
Potential amino acid sequence	MKNKYHLHGAEDLLCMKKSILILHLCILLHRVVGPSICRFSDFHQGANVTVANSL(+) MHKCKIKMLFFMHNRSSAPCR(-) MQSRTSFSPSRENTMLSQRHNRYEELPDTWYKQYAEKFRTWHGKFRSGDKDIPKRTSEGMSNYL KVCSVHKRKRAVFMDDQGHNISVPMSENGPSSKNAGDYSNLTDDTFIPEIRFPADCVPESAIPR TSETSRIYKIEVHGVLDNLPAPVSRNTAMLERFGMMPEYYKKGNKYRGKDGSRVEGKSLSQEQA MLMTRKLVARYLANAGFESGTAVCIDVLSEIIIKHISKLGRNLKLLTDSYRKQFSSIELLKMFL QTVGYSNIGPLMEITKTTNRGANYPMQQDAQVQNQNALLHAQQIFSSMQMIFIFHWKMSRKISI NTICNLEPHLVQAGRTLCYLKDIIDTKSYLTHGTNNMLKNSGHGMASSVQVTKIYQKEHQRECP IT*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0802300_circ_g.1

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