Plant Circular RNA Database

Detail information of Os02g0777700_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0777700_circ_g.2
					
						ID in PlantcircBase
						osa_circ_016758
					
						Alias
						Os02circ28014
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 32909761-32911543  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, PcircRNA_finder, SMALT, Segemehl
					
						Parent gene
						
							Os02g0777700						
					
						Parent gene annotation
						
							Similar to XI-I (Myosin-like protein XI-I); motor/ protein bindi
ng. (Os02t0777700-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/6
					
						Tissues
						panicle/shoot, root, seed, pistil
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0777700-00:5

General Information
CircRNA Name	Os02g0777700_circ_g.2
ID in PlantcircBase	osa_circ_016758
Alias	Os02circ28014
Organism	Oryza sativa
Position	chr2: 32909761-32911543 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, CIRI, PcircRNA_finder, SMALT, Segemehl
Parent gene	Os02g0777700
Parent gene annotation	Similar to XI-I (Myosin-like protein XI-I); motor/ protein bindi ng. (Os02t0777700-00)
Parent gene strand	+
Alternative splicing	NA
Support reads	2/6
Tissues	panicle/shoot, root, seed, pistil
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0777700-00:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATTCAAACAGCAACTAACTGCATGTGTTGAAAAGATTTTTGGGCAACTCAGGGATAATCTGAAA
AAGGAAATATCACCACTTCTTAGTGTCTGCATTCAGaaattcgctacaccgattgggcttccaa
acggcgagcagaagcacggatatgtaagcctattcttttgatcgacgtacagaactgctataga
ttcgtctg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 642
Transcript exons: 32909761-32909811,32909891-32909980,32910137-32910307,32
910404-32910556,32911367-32911543
AAATTCGCTACACCGATTGGGCTTCCAAACGGCGAGCAGAAGCACGGATATGAAACACCACCAG
CAGCAAAATATCTCGCTTCACTTCCACAGAGTTTAACTGGATCAAGGAGAACCAGGATGCCTGT
TGAAAGGCAGGAGGAAAATCATGAAATCTTATTACGATGCATAAAGGAAAATCTAGGATTCAAG
GATGGTAAACCAGTCGCCGCATGCATCATCTACAGTTGCCTTTTACACTGGCGTGCTTTTGAAT
CTGAGAGGACTGCTATTTTTGATCATGTCATTGAAGCCATAAATAATGTTCTCAAGGGGGAAGA
GGCTGACGGTAGATTACCTTATTGGTTGTCCAATACCTCTGCATTGCTATGCCTTCTGCAGAAG
AATTTACGGTCAAATGGATTATTTGCTACACCATCCGGCCGGTCTGGTGGACCTCTAGGGATAG
GTGACAAGATAGTGCAAACACTCAGATCTCCTTCGAAGCTTATGGGACGCATTGATACTCTTGG
ACAAGTGGATGCTCGGTATCCAGCCATATTATTCAAACAGCAACTAACTGCATGTGTTGAAAAG
ATTTTTGGGCAACTCAGGGATAATCTGAAAAAGGAAATATCACCACTTCTTAGTGTCTGCATTC
AG
						
					

						Genomic sequence
						
							AAATTCGCTACACCGATTGGGCTTCCAAACGGCGAGCAGAAGCACGGATATGTAAGCCTATTCT
TTTGATCGACGTACAGAACTGCTATAGATTCGTCTGACATATTGGATCTCAATTTTTGAATTGT
AGGAAACACCACCAGCAGCAAAATATCTCGCTTCACTTCCACAGAGTTTAACTGGATCAAGGAG
AACCAGGATGCCTGTTGAAAGGCAGGAGGTAAATTCCACATCATATTTTTCATTTGTTAATTTT
TGCTTGTTCTAGCTCTGTTGATACCACCTTAGATAAGATGGCAAATAATTTGTTCTGGACGATG
TGACATTATTCAACAAAATTTCTTATGCATCTTGGTGATGATTTTGTGTCATGCAGGAAAATCA
TGAAATCTTATTACGATGCATAAAGGAAAATCTAGGATTCAAGGATGGTAAACCAGTCGCCGCA
TGCATCATCTACAGTTGCCTTTTACACTGGCGTGCTTTTGAATCTGAGAGGACTGCTATTTTTG
ATCATGTCATTGAAGCCATAAATAATGTTCTCAAGGTAAACTTACTTCAGACTCATCTATTTTG
CTGTTTGTGCTAAATGTTTATTTGCGGATGTCAAATAATCATGGAAGTTCTCCTGTTCTTACTC
CAGGGGGAAGAGGCTGACGGTAGATTACCTTATTGGTTGTCCAATACCTCTGCATTGCTATGCC
TTCTGCAGAAGAATTTACGGTCAAATGGATTATTTGCTACACCATCCGGCCGGTCTGGTGGACC
TCTAGGGATAGGTGACAAGATAGTGCAAGTAAGGAACCCTGAAAATTATGGATCATTTTCTTAC
CAGTACTATTGAATTATGCTCAGTGTGCTGATATCCATATGAAGTAATATTTTGAAAGCAATGT
CCTAGTTTAACTCTACATTATTTTATCATGTCTGTATATTTTTTGGACATGACCTGGCTATTGA
CCCAGTCAACAACTCAACGTATTGGGTCACTGGTTGAAGCTGTTGGTCACTGATGGGGCCCCTG
GTTTCATTTTAGCGGCAAAAACAATAGCATGACGGTGCTTAGTAAGGTCTTAAATAGCTTATTC
GGTTCAACTGGAATAAGTAGTAGCTACCTTAGTTGGTTACCAGGCAGATGCAGCACACATTTTT
GGAATCTTTAAGCTGGTGGAGCTAGCCAGCAGTTGTATTAGTCTATGCCGTCAGTTACGCCAAA
ACTACCTGGTTTTAACATAGGTCTTTGGATTTGCCACTTCAAAGTGGCCATCAATCAGATTTGG
TCTGGTTGGACTGTCATGGTGAAAGTGCTGGTTCTCCTCCTCCCTCCTTTCTGACAGGCCACTC
TGCTGTTCTGGGGCAGATCTGGTAAATCATGTGTACCTGTAGAGGATCTATACGCTTCCCATAA
TGGGGGAAATGGAAACATCATGGCATCATGCTCTTCAAGTATTCTTAATTTGCACAACTGTATA
AGCCCAGTAACATGTTTTGCTGGTATAGAAATTTGTTGTGCAATTTTTATTTTCCTTTTTTTTC
GAAAATTCGGGTACGATAAAATTATTGTTTCCCTCTGTTACTGAAATGGCTCCTGTTTTGTTTC
TTGCAGACACTCAGATCTCCTTCGAAGCTTATGGGACGCATTGATACTCTTGGACAAGTGGATG
CTCGGTATCCAGCCATATTATTCAAACAGCAACTAACTGCATGTGTTGAAAAGATTTTTGGGCA
ACTCAGGGATAATCTGAAAAAGGAAATATCACCACTTCTTAGTGTCTGCATTCAG
						
					

			
		

Sequences
Splice junction sequence	ATTCAAACAGCAACTAACTGCATGTGTTGAAAAGATTTTTGGGCAACTCAGGGATAATCTGAAA AAGGAAATATCACCACTTCTTAGTGTCTGCATTCAGaaattcgctacaccgattgggcttccaa acggcgagcagaagcacggatatgtaagcctattcttttgatcgacgtacagaactgctataga ttcgtctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 642 Transcript exons: 32909761-32909811,32909891-32909980,32910137-32910307,32 910404-32910556,32911367-32911543 AAATTCGCTACACCGATTGGGCTTCCAAACGGCGAGCAGAAGCACGGATATGAAACACCACCAG CAGCAAAATATCTCGCTTCACTTCCACAGAGTTTAACTGGATCAAGGAGAACCAGGATGCCTGT TGAAAGGCAGGAGGAAAATCATGAAATCTTATTACGATGCATAAAGGAAAATCTAGGATTCAAG GATGGTAAACCAGTCGCCGCATGCATCATCTACAGTTGCCTTTTACACTGGCGTGCTTTTGAAT CTGAGAGGACTGCTATTTTTGATCATGTCATTGAAGCCATAAATAATGTTCTCAAGGGGGAAGA GGCTGACGGTAGATTACCTTATTGGTTGTCCAATACCTCTGCATTGCTATGCCTTCTGCAGAAG AATTTACGGTCAAATGGATTATTTGCTACACCATCCGGCCGGTCTGGTGGACCTCTAGGGATAG GTGACAAGATAGTGCAAACACTCAGATCTCCTTCGAAGCTTATGGGACGCATTGATACTCTTGG ACAAGTGGATGCTCGGTATCCAGCCATATTATTCAAACAGCAACTAACTGCATGTGTTGAAAAG ATTTTTGGGCAACTCAGGGATAATCTGAAAAAGGAAATATCACCACTTCTTAGTGTCTGCATTC AG
Genomic sequence	AAATTCGCTACACCGATTGGGCTTCCAAACGGCGAGCAGAAGCACGGATATGTAAGCCTATTCT TTTGATCGACGTACAGAACTGCTATAGATTCGTCTGACATATTGGATCTCAATTTTTGAATTGT AGGAAACACCACCAGCAGCAAAATATCTCGCTTCACTTCCACAGAGTTTAACTGGATCAAGGAG AACCAGGATGCCTGTTGAAAGGCAGGAGGTAAATTCCACATCATATTTTTCATTTGTTAATTTT TGCTTGTTCTAGCTCTGTTGATACCACCTTAGATAAGATGGCAAATAATTTGTTCTGGACGATG TGACATTATTCAACAAAATTTCTTATGCATCTTGGTGATGATTTTGTGTCATGCAGGAAAATCA TGAAATCTTATTACGATGCATAAAGGAAAATCTAGGATTCAAGGATGGTAAACCAGTCGCCGCA TGCATCATCTACAGTTGCCTTTTACACTGGCGTGCTTTTGAATCTGAGAGGACTGCTATTTTTG ATCATGTCATTGAAGCCATAAATAATGTTCTCAAGGTAAACTTACTTCAGACTCATCTATTTTG CTGTTTGTGCTAAATGTTTATTTGCGGATGTCAAATAATCATGGAAGTTCTCCTGTTCTTACTC CAGGGGGAAGAGGCTGACGGTAGATTACCTTATTGGTTGTCCAATACCTCTGCATTGCTATGCC TTCTGCAGAAGAATTTACGGTCAAATGGATTATTTGCTACACCATCCGGCCGGTCTGGTGGACC TCTAGGGATAGGTGACAAGATAGTGCAAGTAAGGAACCCTGAAAATTATGGATCATTTTCTTAC CAGTACTATTGAATTATGCTCAGTGTGCTGATATCCATATGAAGTAATATTTTGAAAGCAATGT CCTAGTTTAACTCTACATTATTTTATCATGTCTGTATATTTTTTGGACATGACCTGGCTATTGA CCCAGTCAACAACTCAACGTATTGGGTCACTGGTTGAAGCTGTTGGTCACTGATGGGGCCCCTG GTTTCATTTTAGCGGCAAAAACAATAGCATGACGGTGCTTAGTAAGGTCTTAAATAGCTTATTC GGTTCAACTGGAATAAGTAGTAGCTACCTTAGTTGGTTACCAGGCAGATGCAGCACACATTTTT GGAATCTTTAAGCTGGTGGAGCTAGCCAGCAGTTGTATTAGTCTATGCCGTCAGTTACGCCAAA ACTACCTGGTTTTAACATAGGTCTTTGGATTTGCCACTTCAAAGTGGCCATCAATCAGATTTGG TCTGGTTGGACTGTCATGGTGAAAGTGCTGGTTCTCCTCCTCCCTCCTTTCTGACAGGCCACTC TGCTGTTCTGGGGCAGATCTGGTAAATCATGTGTACCTGTAGAGGATCTATACGCTTCCCATAA TGGGGGAAATGGAAACATCATGGCATCATGCTCTTCAAGTATTCTTAATTTGCACAACTGTATA AGCCCAGTAACATGTTTTGCTGGTATAGAAATTTGTTGTGCAATTTTTATTTTCCTTTTTTTTC GAAAATTCGGGTACGATAAAATTATTGTTTCCCTCTGTTACTGAAATGGCTCCTGTTTTGTTTC TTGCAGACACTCAGATCTCCTTCGAAGCTTATGGGACGCATTGATACTCTTGGACAAGTGGATG CTCGGTATCCAGCCATATTATTCAAACAGCAACTAACTGCATGTGTTGAAAAGATTTTTGGGCA ACTCAGGGATAATCTGAAAAAGGAAATATCACCACTTCTTAGTGTCTGCATTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012359 						
					
						PMCS
						0.346753889

Conservation Information
Conserved circRNAs	osi_circ_012359
PMCS	0.346753889

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32909961-32911540(+)
						
						Potential amino acid sequence
						
							MPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNVLK
GEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGRID
TLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLSVCIQKFATPIGLPNGEQKHGYE
TPPAAKYLASLPQSLTGSRRTRMPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWR
AFESERTAIFDHVIEAINNVLKGEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGP
LGIGDKIVQTLRSPSKLMGRIDTLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLS
VCIQKFATPIGLPNGEQKHGYETPPAAKYLASLPQSLTGSRRTRMPVERQEENHEILLRCIKEN
LGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNVLKGEEADGRLPYWLSNTSALLC
LLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGRIDTLGQVDARYPAILFKQQLTA
CVEKIFGQLRDNLKKEISPLLSVCIQKFATPIGLPNGEQKHGYETPPAAKYLASLPQSLTGSRR
TRMPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNV
LKGEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGR
IDTLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLSVCIQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32909961-32911540(+)
Potential amino acid sequence	MPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNVLK GEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGRID TLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLSVCIQKFATPIGLPNGEQKHGYE TPPAAKYLASLPQSLTGSRRTRMPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWR AFESERTAIFDHVIEAINNVLKGEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGP LGIGDKIVQTLRSPSKLMGRIDTLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLS VCIQKFATPIGLPNGEQKHGYETPPAAKYLASLPQSLTGSRRTRMPVERQEENHEILLRCIKEN LGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNVLKGEEADGRLPYWLSNTSALLC LLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGRIDTLGQVDARYPAILFKQQLTA CVEKIFGQLRDNLKKEISPLLSVCIQKFATPIGLPNGEQKHGYETPPAAKYLASLPQSLTGSRR TRMPVERQEENHEILLRCIKENLGFKDGKPVAACIIYSCLLHWRAFESERTAIFDHVIEAINNV LKGEEADGRLPYWLSNTSALLCLLQKNLRSNGLFATPSGRSGGPLGIGDKIVQTLRSPSKLMGR IDTLGQVDARYPAILFKQQLTACVEKIFGQLRDNLKKEISPLLSVCIQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0777700_circ_g.2

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