Plant Circular RNA Database

Detail information of Os02g0822300_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os02g0822300_circ_g.4
					
						ID in PlantcircBase
						osa_circ_017254
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 35299172-35301256  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0822300						
					
						Parent gene annotation
						
							Similar to RNA-binding protein Nova-1. (Os02t0822300-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0822300_circ_g.1 Os02g0822300_circ_g.2 Os02g0822300_circ_g.3 Os02g0822300_circ_g.5 Os02g0822300_circ_g.6 Os02g0822300_circ_g.7 Os02g0822300_circ_g.8 						
					
						Support reads
						1
					
						Tissues
						shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0822300-00:5

General Information
CircRNA Name	Os02g0822300_circ_g.4
ID in PlantcircBase	osa_circ_017254
Alias	NA
Organism	Oryza sativa
Position	chr2: 35299172-35301256 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0822300
Parent gene annotation	Similar to RNA-binding protein Nova-1. (Os02t0822300-00)
Parent gene strand	+
Alternative splicing	Os02g0822300_circ_g.1 Os02g0822300_circ_g.2 Os02g0822300_circ_g.3 Os02g0822300_circ_g.5 Os02g0822300_circ_g.6 Os02g0822300_circ_g.7 Os02g0822300_circ_g.8
Support reads	1
Tissues	shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0822300-00:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCGTA
GGACGTGCCGGAAGGAACATTACAGAGATCATTCAGatgacaaggagaagccaacccatctgag
gtttctggtatcaaacacggcagcaggatgcattattggcaagggtggatcaaccattaatgaa
tttcagtc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 806
Transcript exons: 35299172-35299410,35299496-35299596,35299897-35300090,35
300392-35300537,35301131-35301256
ATGACAAGGAGAAGCCAACCCATCTGAGGTTTCTGGTATCAAACACGGCAGCAGGATGCATTAT
TGGCAAGGGTGGATCAACCATTAATGAATTTCAGTCCCAGTCTGGGGCTCGTATTCAGCTATCG
CGTAGCCATGAGTTCTTCCCTGGTACAAATGACAGAATTATCATGGTTTCTGGTCTGTTTGATG
AAGTAATAAAGGCCATGGAGCTGATTCTCGAGAAGCTTTTAGCTGAGGGGGAAGAAAGCAATGA
AGCTGAAGCTAGACCTAAAGTTAGACTTGTAGTTCCTAACAGTTCTTGTGGTGGAATCATTGGT
AAAGGAGGGTCAACAATCAAGTCATTCATTGAAGACTCACATGCTGGAATCAAGATTTCACCAC
AGGATAACAATTTTGTTGGTTTGCATGATAGACTCGTTACTGTCACAGGACCCTTGGACCATCA
GATGCGTGCAATATATTTGATATTAAGCAAGTTATCGGAGGACGTTCACTACCCACCTAATCTG
AGTTCCCCATTTCCATATGCAGGCCTTGGTTTCCCTAGTTACCCTGGTGTTCCTGTTGGGTATA
TGATCCCACAGGTGCCGTATAATAATGCTGTGAACTATGGACCTAATGGGTATGGAGGAAGGTA
CCAAAACAACAAGGTTAGTGATTGCTGCTGCTTTACTCTTCCCAGTACACCTATGAGATCACCT
GCTAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCG
TAGGACGTGCCGGAAGGAACATTACAGAGATCATTCAG
						
					

						Genomic sequence
						
							ATGACAAGGAGAAGCCAACCCATCTGAGGTTTCTGGTATCAAACACGGCAGCAGGATGCATTAT
TGGCAAGGGTGGATCAACCATTAATGAATTTCAGTCCCAGTCTGGGGCTCGTATTCAGCTATCG
CGTAGCCATGAGTTCTTCCCTGGTACAAATGACAGAATTATCATGGTTTCTGGTCTGTTTGATG
AAGTAATAAAGGCCATGGAGCTGATTCTCGAGAAGCTTTTAGCTGAGGTAAACTATTTTATTCC
GCAAATAGTTGTATCATAGGGCCATGTTACTTTATTTGATGATTGGTATTCTATGGTTACTTTC
CCAGGGGGAAGAAAGCAATGAAGCTGAAGCTAGACCTAAAGTTAGACTTGTAGTTCCTAACAGT
TCTTGTGGTGGAATCATTGGTAAAGGAGGGTCAACAATCAAGTATGTACCATTGATAAATTTGT
TTCTGGTTTCTGAAATCTTATTTTTATTCTTATTCCCATTGCGCTTCTTCATTTTCTTAGTTGG
TTATAATGTATGTATACCATGAAGCGTGTCTGAACCGAATGAGGAAATGCATGGTTATTTTCCT
AATGCACTCATCTTTGACTTAACTGCACCCAAGTTATTGCATGCTCTCTTGTCAATTGTACGCA
AGCAGTATTTGTTGTTTCATTTGTATTGTTTTGTGTTGGTTTATGTTTCTTTGAATGTGAGTTG
ACATGCTTGTTTTCTCATCAGGTCATTCATTGAAGACTCACATGCTGGAATCAAGATTTCACCA
CAGGATAACAATTTTGTTGGTTTGCATGATAGACTCGTTACTGTCACAGGACCCTTGGACCATC
AGATGCGTGCAATATATTTGATATTAAGCAAGTTATCGGAGGACGTTCACTACCCACCTAATCT
GAGTTCCCCATTTCCATATGCAGGTAAACAAATTTCAATATTTGATTTGGTGTCAAACGGCTAC
TTTTCTTATTATGGGGTGCCTTAATTCTCGGACTGCATTTCTTTTCTGGAATTCATATACTGCA
TTGTATAGGTCACAAATGAACATGCTTGGTCACTCGTACCATTTGGCAGCCATGAGCTTTCTGC
ATGAATTTTTATTCAGCTACTGCACGATTGTTCTCTTTCCGTTTCAGTGATCATGTTGAATGCG
TTCACATGCTATGTCCATTCAGTTACTGCATGATCATTTTATCTGAATTCCTCTTCTTTTGTTT
TCAGGCCTTGGTTTCCCTAGTTACCCTGGTGTTCCTGTTGGGTATATGATCCCACAGGTGCCGT
ATAATAATGCTGTGAACTATGGACCTAATGGGTATGGAGGAAGGTACCAAAACAACAAGGTTAG
TGATTGCTGCTGCTTTACTCTTGTCTTGTATATCACTAAAGCAAACCTTTTGAATTCAATATCA
GCATTTTTATGCACATGTTGGTATGAGCTAACATGTGTTCAATTTTGTTATGCACTAGATTAAG
GTTATGTTCAAGGTGATTTGCATTAGATGTTCTATAATAGAAATACTATTTTCTGGTTGTGGTT
TTGTACTTTTGTCATACATCACTATAGGCATCTAGAATTTCTGCTTCAAACCAACCTTAACAAT
AGTAAGACTCATTGGATGAGATGTCTGAATTACTAATTGTGGTTTATTTGAGTGACATATTCCA
TCCATTTGTTCATCCATAGTATGCTTTAGTACATGTTAGTGCAAAGCATAAGACAGCTTTTAAC
GAATTGAAGTATTATTACCTATGTCATGCATGATGTTTTTCACCGTAAGTAACATCAGTGCTGT
AAATTCTAATTCAGAACTGTAAGTAACGTCATTGTACTGCTTTCTACTGAATTCCACAAACATT
ACCATTCACTATTTTTCCTTTTTTTCCCTTTGAGTGAAATTTGATATATGCGTTCAGAGCTTCA
AATTAATCCAACAATTGTCTGCTGTTGAAAATTTTGCAGCCCAGTACACCTATGAGATCACCTG
CTAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCGT
AGGACGTGCCGGAAGGAACATTACAGAGATCATTCAG
						
					

			
		

Sequences
Splice junction sequence	TAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCGTA GGACGTGCCGGAAGGAACATTACAGAGATCATTCAGatgacaaggagaagccaacccatctgag gtttctggtatcaaacacggcagcaggatgcattattggcaagggtggatcaaccattaatgaa tttcagtc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 806 Transcript exons: 35299172-35299410,35299496-35299596,35299897-35300090,35 300392-35300537,35301131-35301256 ATGACAAGGAGAAGCCAACCCATCTGAGGTTTCTGGTATCAAACACGGCAGCAGGATGCATTAT TGGCAAGGGTGGATCAACCATTAATGAATTTCAGTCCCAGTCTGGGGCTCGTATTCAGCTATCG CGTAGCCATGAGTTCTTCCCTGGTACAAATGACAGAATTATCATGGTTTCTGGTCTGTTTGATG AAGTAATAAAGGCCATGGAGCTGATTCTCGAGAAGCTTTTAGCTGAGGGGGAAGAAAGCAATGA AGCTGAAGCTAGACCTAAAGTTAGACTTGTAGTTCCTAACAGTTCTTGTGGTGGAATCATTGGT AAAGGAGGGTCAACAATCAAGTCATTCATTGAAGACTCACATGCTGGAATCAAGATTTCACCAC AGGATAACAATTTTGTTGGTTTGCATGATAGACTCGTTACTGTCACAGGACCCTTGGACCATCA GATGCGTGCAATATATTTGATATTAAGCAAGTTATCGGAGGACGTTCACTACCCACCTAATCTG AGTTCCCCATTTCCATATGCAGGCCTTGGTTTCCCTAGTTACCCTGGTGTTCCTGTTGGGTATA TGATCCCACAGGTGCCGTATAATAATGCTGTGAACTATGGACCTAATGGGTATGGAGGAAGGTA CCAAAACAACAAGGTTAGTGATTGCTGCTGCTTTACTCTTCCCAGTACACCTATGAGATCACCT GCTAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCG TAGGACGTGCCGGAAGGAACATTACAGAGATCATTCAG
Genomic sequence	ATGACAAGGAGAAGCCAACCCATCTGAGGTTTCTGGTATCAAACACGGCAGCAGGATGCATTAT TGGCAAGGGTGGATCAACCATTAATGAATTTCAGTCCCAGTCTGGGGCTCGTATTCAGCTATCG CGTAGCCATGAGTTCTTCCCTGGTACAAATGACAGAATTATCATGGTTTCTGGTCTGTTTGATG AAGTAATAAAGGCCATGGAGCTGATTCTCGAGAAGCTTTTAGCTGAGGTAAACTATTTTATTCC GCAAATAGTTGTATCATAGGGCCATGTTACTTTATTTGATGATTGGTATTCTATGGTTACTTTC CCAGGGGGAAGAAAGCAATGAAGCTGAAGCTAGACCTAAAGTTAGACTTGTAGTTCCTAACAGT TCTTGTGGTGGAATCATTGGTAAAGGAGGGTCAACAATCAAGTATGTACCATTGATAAATTTGT TTCTGGTTTCTGAAATCTTATTTTTATTCTTATTCCCATTGCGCTTCTTCATTTTCTTAGTTGG TTATAATGTATGTATACCATGAAGCGTGTCTGAACCGAATGAGGAAATGCATGGTTATTTTCCT AATGCACTCATCTTTGACTTAACTGCACCCAAGTTATTGCATGCTCTCTTGTCAATTGTACGCA AGCAGTATTTGTTGTTTCATTTGTATTGTTTTGTGTTGGTTTATGTTTCTTTGAATGTGAGTTG ACATGCTTGTTTTCTCATCAGGTCATTCATTGAAGACTCACATGCTGGAATCAAGATTTCACCA CAGGATAACAATTTTGTTGGTTTGCATGATAGACTCGTTACTGTCACAGGACCCTTGGACCATC AGATGCGTGCAATATATTTGATATTAAGCAAGTTATCGGAGGACGTTCACTACCCACCTAATCT GAGTTCCCCATTTCCATATGCAGGTAAACAAATTTCAATATTTGATTTGGTGTCAAACGGCTAC TTTTCTTATTATGGGGTGCCTTAATTCTCGGACTGCATTTCTTTTCTGGAATTCATATACTGCA TTGTATAGGTCACAAATGAACATGCTTGGTCACTCGTACCATTTGGCAGCCATGAGCTTTCTGC ATGAATTTTTATTCAGCTACTGCACGATTGTTCTCTTTCCGTTTCAGTGATCATGTTGAATGCG TTCACATGCTATGTCCATTCAGTTACTGCATGATCATTTTATCTGAATTCCTCTTCTTTTGTTT TCAGGCCTTGGTTTCCCTAGTTACCCTGGTGTTCCTGTTGGGTATATGATCCCACAGGTGCCGT ATAATAATGCTGTGAACTATGGACCTAATGGGTATGGAGGAAGGTACCAAAACAACAAGGTTAG TGATTGCTGCTGCTTTACTCTTGTCTTGTATATCACTAAAGCAAACCTTTTGAATTCAATATCA GCATTTTTATGCACATGTTGGTATGAGCTAACATGTGTTCAATTTTGTTATGCACTAGATTAAG GTTATGTTCAAGGTGATTTGCATTAGATGTTCTATAATAGAAATACTATTTTCTGGTTGTGGTT TTGTACTTTTGTCATACATCACTATAGGCATCTAGAATTTCTGCTTCAAACCAACCTTAACAAT AGTAAGACTCATTGGATGAGATGTCTGAATTACTAATTGTGGTTTATTTGAGTGACATATTCCA TCCATTTGTTCATCCATAGTATGCTTTAGTACATGTTAGTGCAAAGCATAAGACAGCTTTTAAC GAATTGAAGTATTATTACCTATGTCATGCATGATGTTTTTCACCGTAAGTAACATCAGTGCTGT AAATTCTAATTCAGAACTGTAAGTAACGTCATTGTACTGCTTTCTACTGAATTCCACAAACATT ACCATTCACTATTTTTCCTTTTTTTCCCTTTGAGTGAAATTTGATATATGCGTTCAGAGCTTCA AATTAATCCAACAATTGTCTGCTGTTGAAAATTTTGCAGCCCAGTACACCTATGAGATCACCTG CTAACAATGATGCCCAAGATTCTCTCACTATTGGTATTGCTGATGAACATATTGGTGCTGTCGT AGGACGTGCCGGAAGGAACATTACAGAGATCATTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012497 zma_circ_001754 						
					
						PMCS
						0.348206599

Conservation Information
Conserved circRNAs	osi_circ_012497 zma_circ_001754
PMCS	0.348206599

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							35299343-35299195(+)
35299258-35301251(-)
						
						Potential amino acid sequence
						
							MVSGLFDEVIKAMELILEKLLAEGEESNEAEARPKVRLVVPNSSCGGIIGKGGSTIKSFIEDSH
AGIKISPQDNNFVGLHDRLVTVTGPLDHQMRAIYLILSKLSEDVHYPPNLSSPFPYAGLGFPSY
PGVPVGYMIPQVPYNNAVNYGPNGYGGRYQNNKVSDCCCFTLPSTPMRSPANNDAQDSLTIGIA
DEHIGAVVGRAGRNITEIIQMTRRSQPI*(+)
MVDPPLPIMHPAAVFDTRNLRWVGFSLSSE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	35299343-35299195(+) 35299258-35301251(-)
Potential amino acid sequence	MVSGLFDEVIKAMELILEKLLAEGEESNEAEARPKVRLVVPNSSCGGIIGKGGSTIKSFIEDSH AGIKISPQDNNFVGLHDRLVTVTGPLDHQMRAIYLILSKLSEDVHYPPNLSSPFPYAGLGFPSY PGVPVGYMIPQVPYNNAVNYGPNGYGGRYQNNKVSDCCCFTLPSTPMRSPANNDAQDSLTIGIA DEHIGAVVGRAGRNITEIIQMTRRSQPI(+) MVDPPLPIMHPAAVFDTRNLRWVGFSLSSE(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0822300_circ_g.4

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