Plant Circular RNA Database

Detail information of Os02g0665000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0665000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_015869
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 26992312-26993274  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0665000						
					
						Parent gene annotation
						
							Similar to Malic enzyme. (Os02t0665000-01);Similar to NADP-malic
 enzyme. (Os02t0665000-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0665000-01:3
Os02t0665000-02:3

General Information
CircRNA Name	Os02g0665000_circ_g.2
ID in PlantcircBase	osa_circ_015869
Alias	NA
Organism	Oryza sativa
Position	chr2: 26992312-26993274 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0665000
Parent gene annotation	Similar to Malic enzyme. (Os02t0665000-01);Similar to NADP-malic enzyme. (Os02t0665000-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0665000-01:3 Os02t0665000-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ACTTCTGGCATGCCTCTCCGACCGTCGGCGTGTACACCACCGGCAGCAGCTCCTCCACGTTGTC
GATCAGGAGCTTGTAGAACAGCCTCTCACTCCTCTCctggttttggggttgtccacttgtccag
cggtgatgcgacagcgacaatgctcatcttgctggctcatcgacaggaactcgctcgtcgtgtg
aatcatga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 726
Transcript exons: 26992312-26992779,26992908-26993013,26993123-26993274
CTGGTTTTGGGGTTGTCCACTTGTCCAGCGGTGATGCGACAGCGACAATGCTCATCTTGCTGGC
TCATCGACAGGAACTCGCTCGTCGTGTGAATCATGAGGGCAAGGAGGCATGACGGTGTGAGGGA
AACCAGTCGACGGGGCAATCCGGCGGCGGCATGACGCTAACCGAATGGATCAGGGATGATCTGC
AAGAGGGCGGCTGCAAACAAATCCAGAAGATGCAGCGATGTGGAAGGAGGTGACCCACGAGGGA
ATGATGAGGGGCGCGATGCGGTGAGGGTGGAGGGAAGCGACGCGCAGGGGAGACAAGGCGAAGC
GCAGAGGGAAACGATGCGTGAGGGAGGGGGCGAGGACGGAGGTGATGGCGGCGATGATGGTGGC
AGACGGGTGAAGGTGGAGGCAAGGCGCGCGAACTCCTCCTAGGGCAGTGTACAGAGACAGTTTG
CCGACAGGAATACCCATTCCCTGACAACCAAGATCTCCAAGGCCAAGAATGCGCTCACCGTCGG
TGATGACGATCACCTGGATGCTCCTCTCCGGCCAGTTCTTGAGCACCTCAAGGATCTTCCCCTT
GTCCTTGAGGCTGATGTACAGGCCCTGTGGCCGCCTGTAGATGCTGCCGTACTTCTGGCATGCC
TCTCCGACCGTCGGCGTGTACACCACCGGCAGCAGCTCCTCCACGTTGTCGATCAGGAGCTTGT
AGAACAGCCTCTCACTCCTCTC
						
					

						Genomic sequence
						
							CTGGTTTTGGGGTTGTCCACTTGTCCAGCGGTGATGCGACAGCGACAATGCTCATCTTGCTGGC
TCATCGACAGGAACTCGCTCGTCGTGTGAATCATGAGGGCAAGGAGGCATGACGGTGTGAGGGA
AACCAGTCGACGGGGCAATCCGGCGGCGGCATGACGCTAACCGAATGGATCAGGGATGATCTGC
AAGAGGGCGGCTGCAAACAAATCCAGAAGATGCAGCGATGTGGAAGGAGGTGACCCACGAGGGA
ATGATGAGGGGCGCGATGCGGTGAGGGTGGAGGGAAGCGACGCGCAGGGGAGACAAGGCGAAGC
GCAGAGGGAAACGATGCGTGAGGGAGGGGGCGAGGACGGAGGTGATGGCGGCGATGATGGTGGC
AGACGGGTGAAGGTGGAGGCAAGGCGCGCGAACTCCTCCTAGGGCAGTGTACAGAGACAGTTTG
CCGACAGGAATACCCATTCCCTGTACATAATAACAGCAGCACACATCAGCAAATGCTTTCAGGT
AAACATACATCATCACAGCTAAATCTTTTCGACATATTCATGACAGCAAAATCAGGGCAGGAAA
TGGGGGAAAAGATGCAGTACCTGACAACCAAGATCTCCAAGGCCAAGAATGCGCTCACCGTCGG
TGATGACGATCACCTGGATGCTCCTCTCCGGCCAGTTCTTGAGCACCTCAAGGATCTTCCCCCT
GAATCCACCAACAAGCAAACCAACAAACACAGATTCCTGTCAGGCTGCAAATTCCTAATCAACC
TCAACATCAGAAGGAAAACAGAATCCTCGCAAGAAAACCGCACTTGTCCTTGAGGCTGATGTAC
AGGCCCTGTGGCCGCCTGTAGATGCTGCCGTACTTCTGGCATGCCTCTCCGACCGTCGGCGTGT
ACACCACCGGCAGCAGCTCCTCCACGTTGTCGATCAGGAGCTTGTAGAACAGCCTCTCACTCCT
CTC
						
					

			
		

Sequences
Splice junction sequence	ACTTCTGGCATGCCTCTCCGACCGTCGGCGTGTACACCACCGGCAGCAGCTCCTCCACGTTGTC GATCAGGAGCTTGTAGAACAGCCTCTCACTCCTCTCctggttttggggttgtccacttgtccag cggtgatgcgacagcgacaatgctcatcttgctggctcatcgacaggaactcgctcgtcgtgtg aatcatga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 726 Transcript exons: 26992312-26992779,26992908-26993013,26993123-26993274 CTGGTTTTGGGGTTGTCCACTTGTCCAGCGGTGATGCGACAGCGACAATGCTCATCTTGCTGGC TCATCGACAGGAACTCGCTCGTCGTGTGAATCATGAGGGCAAGGAGGCATGACGGTGTGAGGGA AACCAGTCGACGGGGCAATCCGGCGGCGGCATGACGCTAACCGAATGGATCAGGGATGATCTGC AAGAGGGCGGCTGCAAACAAATCCAGAAGATGCAGCGATGTGGAAGGAGGTGACCCACGAGGGA ATGATGAGGGGCGCGATGCGGTGAGGGTGGAGGGAAGCGACGCGCAGGGGAGACAAGGCGAAGC GCAGAGGGAAACGATGCGTGAGGGAGGGGGCGAGGACGGAGGTGATGGCGGCGATGATGGTGGC AGACGGGTGAAGGTGGAGGCAAGGCGCGCGAACTCCTCCTAGGGCAGTGTACAGAGACAGTTTG CCGACAGGAATACCCATTCCCTGACAACCAAGATCTCCAAGGCCAAGAATGCGCTCACCGTCGG TGATGACGATCACCTGGATGCTCCTCTCCGGCCAGTTCTTGAGCACCTCAAGGATCTTCCCCTT GTCCTTGAGGCTGATGTACAGGCCCTGTGGCCGCCTGTAGATGCTGCCGTACTTCTGGCATGCC TCTCCGACCGTCGGCGTGTACACCACCGGCAGCAGCTCCTCCACGTTGTCGATCAGGAGCTTGT AGAACAGCCTCTCACTCCTCTC
Genomic sequence	CTGGTTTTGGGGTTGTCCACTTGTCCAGCGGTGATGCGACAGCGACAATGCTCATCTTGCTGGC TCATCGACAGGAACTCGCTCGTCGTGTGAATCATGAGGGCAAGGAGGCATGACGGTGTGAGGGA AACCAGTCGACGGGGCAATCCGGCGGCGGCATGACGCTAACCGAATGGATCAGGGATGATCTGC AAGAGGGCGGCTGCAAACAAATCCAGAAGATGCAGCGATGTGGAAGGAGGTGACCCACGAGGGA ATGATGAGGGGCGCGATGCGGTGAGGGTGGAGGGAAGCGACGCGCAGGGGAGACAAGGCGAAGC GCAGAGGGAAACGATGCGTGAGGGAGGGGGCGAGGACGGAGGTGATGGCGGCGATGATGGTGGC AGACGGGTGAAGGTGGAGGCAAGGCGCGCGAACTCCTCCTAGGGCAGTGTACAGAGACAGTTTG CCGACAGGAATACCCATTCCCTGTACATAATAACAGCAGCACACATCAGCAAATGCTTTCAGGT AAACATACATCATCACAGCTAAATCTTTTCGACATATTCATGACAGCAAAATCAGGGCAGGAAA TGGGGGAAAAGATGCAGTACCTGACAACCAAGATCTCCAAGGCCAAGAATGCGCTCACCGTCGG TGATGACGATCACCTGGATGCTCCTCTCCGGCCAGTTCTTGAGCACCTCAAGGATCTTCCCCCT GAATCCACCAACAAGCAAACCAACAAACACAGATTCCTGTCAGGCTGCAAATTCCTAATCAACC TCAACATCAGAAGGAAAACAGAATCCTCGCAAGAAAACCGCACTTGTCCTTGAGGCTGATGTAC AGGCCCTGTGGCCGCCTGTAGATGCTGCCGTACTTCTGGCATGCCTCTCCGACCGTCGGCGTGT ACACCACCGGCAGCAGCTCCTCCACGTTGTCGATCAGGAGCTTGTAGAACAGCCTCTCACTCCT CTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004159* 						
					
						PMCS
						0.269270388

Conservation Information
Conserved circRNAs	osi_circ_004159*
PMCS	0.269270388

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26993185-26992424(+)
26992411-26993243(-)
26992368-26993266(-)
						
						Potential amino acid sequence
						
							MPLRPSACTPPAAAPPRCRSGACRTASHSSPGFGVVHLSSGDATATMLILLAHRQELARRVNHE
GKEA*(+)
MIHTTSEFLSMSQQDEHCRCRITAGQVDNPKTRRGVRGCSTSS*(-)
MSIVAVASPLDKWTTPKPGEE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26993185-26992424(+) 26992411-26993243(-) 26992368-26993266(-)
Potential amino acid sequence	MPLRPSACTPPAAAPPRCRSGACRTASHSSPGFGVVHLSSGDATATMLILLAHRQELARRVNHE GKEA(+) MIHTTSEFLSMSQQDEHCRCRITAGQVDNPKTRRGVRGCSTSS(-) MSIVAVASPLDKWTTPKPGEE*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0665000_circ_g.2

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