Plant Circular RNA Database

Detail information of Os06g0716700_circ_g.7

			
						 General Information
					
						CircRNA Name
						Os06g0716700_circ_g.7
					
						ID in PlantcircBase
						osa_circ_032356
					
						Alias
						Os_ciR10644
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 30446264-30446644  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0716700						
					
						Parent gene annotation
						
							Similar to Heat shock protein 90. (Os06t0716700-01);Similar to H
eat shock protein 90. (Os06t0716700-02);Similar to Heat shock pr
otein 90. (Os06t0716700-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0716700_circ_g.4 Os06g0716700_circ_g.5 Os06g0716700_circ_g.6 Os06g0716700_circ_g.8 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0716700-01:1
Os06t0716700-03:2
Os06t0716700-02:2

General Information
CircRNA Name	Os06g0716700_circ_g.7
ID in PlantcircBase	osa_circ_032356
Alias	Os_ciR10644
Organism	Oryza sativa
Position	chr6: 30446264-30446644 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0716700
Parent gene annotation	Similar to Heat shock protein 90. (Os06t0716700-01);Similar to H eat shock protein 90. (Os06t0716700-02);Similar to Heat shock pr otein 90. (Os06t0716700-03)
Parent gene strand	-
Alternative splicing	Os06g0716700_circ_g.4 Os06g0716700_circ_g.5 Os06g0716700_circ_g.6 Os06g0716700_circ_g.8
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0716700-01:1 Os06t0716700-03:2 Os06t0716700-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGCTATGTTGTTGGAGCATTTCTCGTGA
TACATTGAGAGGCAATGTGTCCGAGTCGACGAGACCctttcaaatctcagaagctttgcaagac
ggttcctgttagttgcgtcttcaatgatgcccaacttgactgattttccaaactcattccagaa
cttggcat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 305
Transcript exons: 30446264-30446402,30446479-30446644
CTTTCAAATCTCAGAAGCTTTGCAAGACGGTTCCTGTTAGTTGCGTCTTCAATGATGCCCAACT
TGACTGATTTTCCAAACTCATTCCAGAACTTGGCATACTGGCCCTTTTTCTCCTCCATTGCACT
TTTTTCTTCATCTGTCTTATCTTTGTTGCTGTACTCATCAGGATCTTCCTCAGCAAGTTTTCTA
ATCATGTCAAGAGCTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGCTATGTTGTTGGA
GCATTTCTCGTGATACATTGAGAGGCAATGTGTCCGAGTCGACGAGACC
						
						Genomic sequence
						
							CTTTCAAATCTCAGAAGCTTTGCAAGACGGTTCCTGTTAGTTGCGTCTTCAATGATGCCCAACT
TGACTGATTTTCCAAACTCATTCCAGAACTTGGCATACTGGCCCTTTTTCTCCTCCATTGCACT
TTTTTCTTCATCTGAGTACAGAAGATTATGTGAGAATTACATCATAAATAACAGTATATGACCT
GTGATCCACAGAGAACTTGTTACCTGTCTTATCTTTGTTGCTGTACTCATCAGGATCTTCCTCA
GCAAGTTTTCTAATCATGTCAAGAGCTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGC
TATGTTGTTGGAGCATTTCTCGTGATACATTGAGAGGCAATGTGTCCGAGTCGACGAGACC

Sequences
Splice junction sequence	CTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGCTATGTTGTTGGAGCATTTCTCGTGA TACATTGAGAGGCAATGTGTCCGAGTCGACGAGACCctttcaaatctcagaagctttgcaagac ggttcctgttagttgcgtcttcaatgatgcccaacttgactgattttccaaactcattccagaa cttggcat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 305 Transcript exons: 30446264-30446402,30446479-30446644 CTTTCAAATCTCAGAAGCTTTGCAAGACGGTTCCTGTTAGTTGCGTCTTCAATGATGCCCAACT TGACTGATTTTCCAAACTCATTCCAGAACTTGGCATACTGGCCCTTTTTCTCCTCCATTGCACT TTTTTCTTCATCTGTCTTATCTTTGTTGCTGTACTCATCAGGATCTTCCTCAGCAAGTTTTCTA ATCATGTCAAGAGCTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGCTATGTTGTTGGA GCATTTCTCGTGATACATTGAGAGGCAATGTGTCCGAGTCGACGAGACC
Genomic sequence	CTTTCAAATCTCAGAAGCTTTGCAAGACGGTTCCTGTTAGTTGCGTCTTCAATGATGCCCAACT TGACTGATTTTCCAAACTCATTCCAGAACTTGGCATACTGGCCCTTTTTCTCCTCCATTGCACT TTTTTCTTCATCTGAGTACAGAAGATTATGTGAGAATTACATCATAAATAACAGTATATGACCT GTGATCCACAGAGAACTTGTTACCTGTCTTATCTTTGTTGCTGTACTCATCAGGATCTTCCTCA GCAAGTTTTCTAATCATGTCAAGAGCTTTGCGGATTAGTTTCTTCTTGATGGTCTTCAGGCTGC TATGTTGTTGGAGCATTTCTCGTGATACATTGAGAGGCAATGTGTCCGAGTCGACGAGACC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006954* osi_circ_016625 						
					
						PMCS
						0.577353208

Conservation Information
Conserved circRNAs	osi_circ_006954* osi_circ_016625
PMCS	0.577353208

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							30446316-30446300(+)
30446624-30446331(+)
30446601-30446577(-)
						
						Potential amino acid sequence
						
							MMPNLTDFPNSFQNLAYWPFFSSIALFSSSVLSLLLYSSGSSSASFLIMSRALRISFFLMVFRL
LCCWSISRDTLRGNVSESTRPFQISEALQDGSC*(+)
MCPSRRDPFKSQKLCKTVPVSCVFNDAQLD*(+)
MLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDEYSNKDKTDEEKSAMEEKKGQYAKFWNEFGKS
VKLGIIEDATNRNRLAKLLRFERVSSTRTHCLSMYHEKCSNNIAA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	30446316-30446300(+) 30446624-30446331(+) 30446601-30446577(-)
Potential amino acid sequence	MMPNLTDFPNSFQNLAYWPFFSSIALFSSSVLSLLLYSSGSSSASFLIMSRALRISFFLMVFRL LCCWSISRDTLRGNVSESTRPFQISEALQDGSC(+) MCPSRRDPFKSQKLCKTVPVSCVFNDAQLD(+) MLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDEYSNKDKTDEEKSAMEEKKGQYAKFWNEFGKS VKLGIIEDATNRNRLAKLLRFERVSSTRTHCLSMYHEKCSNNIAA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0716700_circ_g.7

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