Plant Circular RNA Database

Detail information of Os03g0654400_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0654400_circ_g.2
					
						ID in PlantcircBase
						osa_circ_020843
					
						Alias
						Os_ciR4208
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 25505985-25508038  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os03g0654400						
					
						Parent gene annotation
						
							Similar to Cationic amino acid transporter-like protein. (Os03t0
654400-01);Similar to High-affinity cationic amino acid transpor
ter 1. (Os03t0654400-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						8
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0654400-01:7
Os03t0654400-02:7

General Information
CircRNA Name	Os03g0654400_circ_g.2
ID in PlantcircBase	osa_circ_020843
Alias	Os_ciR4208
Organism	Oryza sativa
Position	chr3: 25505985-25508038 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os03g0654400
Parent gene annotation	Similar to Cationic amino acid transporter-like protein. (Os03t0 654400-01);Similar to High-affinity cationic amino acid transpor ter 1. (Os03t0654400-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	8
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0654400-01:7 Os03t0654400-02:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAAAACGACACTTACAAGATTGGGGGATATGCCACGGGCAACAGCAGATCCACCAATAGTATAT
TCCAGCACTAAAGCCCAACCAATCAACCAGGCAACAcattcctgccagctgggaaacatccatg
aaaaaagccagagctgctgcacagagaccagtcacgactgtgctctttacaggaacttgtgtcc
tcttgtta

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CATTCCTGCCAGCTGGGAAACATCCATGAAAAAAGCCAGAGCTGCTGCACAGAGACCAGTCACG
ACTGTGCTCTTTACAGGAACTTGTGTCCTCTTGTTAACATCGGCGAAGAAAGATGGCAGCAGCC
CGTCTCGTGCCATCGCCATTAGTATTCTAGGCTGCATGAAATGGCAACTAAAATCTTTAAAATG
TATTTTAAAAAACAAGGTAATGCATCCCCAAGTTCTCAGTTTCACATAATAATGTGCAAGGAAA
GAGTGGGCGGGTGGGTGTCTGTTTCTAAGTTTATGTTTTTGTGTTCTCATTTGAGTTTGTCTGT
GTAGGCATGGGATCAGTTTTACGTATAAGGCAATCAGGGATAGCTCAAATGTAACAAGTACTGG
TATACATGCCACATCATCAATCTAGTGTTGATGTGTGTGCTACAAATTTCATTTATGTTGATAT
GGTATTAGTTCAATATACATTTGCAGGTACTATAGTTTATTTTGTCAAAACAGAGCCTATCGGC
AAAATTGCAAGGAGAGAAACAACGTTAATCATTATATGAATAGTTTAATTAAAATTCAAAAATC
AGAATGCAAGTGAAAAGTGCAATCAACTGCTCGCTAATGTACTGCAGAATAATTGACCTTATGG
ACCAAGCAGTTTGATTCAGATTCAGAAATCAGAATACAAGTGAGAAGTACAACAAAAAAATAAC
TTTTTTTCCACAAGGATTATAGATTCAGCTTTGGCTAGTTAGAAGACCACTCTCACTAATATAA
TGCGGAATAATTGACTGTGATATTAGAACGCAAATTCCTTATGGACCAAGCATATAGTAGACGA
CTAGAATCAACTCTATGTGCTCACTGTCTTGTTTAAAGCTTGGGAATTATTAGGATTTTTTTTT
ATATCGAGGGATGTGTTTGTATACCTGCGGAAGAAGTGACCCCAACAAAGTTGAACAAAGTGCA
AGAACAGCACCACTTGTCACAATATACCTAAAACAAAACAGCATAGTGACAGACTAAATAAGCA
CGTTGCAAGACCATTATAGATAATTGGATATATGGGAATAAACAAAAGGAGAGCGAGTCCTTAC
ATGGCCCACTGCATTCCATGTTTCGCAAAGACAGATGAAATGGGCGTATCTGGGTCCATAGCGA
AATATGGTACCAGCCCAACAATAACAACAGAAACCATCATGTACAAGCAACAGCAGATAGACAA
TGCCGCTCCTATTCCCAGTGGTAGATCTCTCTGTGGATTCTTCACCTATAAGGGTTGAAAGAGG
GAAAAATCAGCATCCCCATACAGGAAGTGGACGACATGGTTAGTTAGGATCATCTTAACCTCCT
CAGCAGTACTAGCAACTGTATCAAAGCCAATATATGCAAAGAAAACAGTTGCTGATCCAGCAAG
CATTCCATTTATCCCATGTGGAAAATATCTGAAATGTTCAAATGGTAAGTGCATCAGTAATTAC
TCTCAAAATACAATCAACTGTTTTTTTTTTTTTGCATTACCCATCAGTAACTTTATAGCCAACC
CATCCTATCTGGAAGCCGATATAACTACCAGCTACAATAACAAATATCATCACACAAGCATTCA
GAACTGTTATAAGCTCTTGTACAGCTGAACTCTGCAACAGATTCAAATAGAATAATTAGTAACA
GTCCTATAATCCTAGTTTGCAGATGAAAGAATAGGAAACTTACAAATCGACCAATGTAACAACC
TCTTTTATCCCAACACATAGCAAAACAGTAACAACAAAAACGAGGGCAGCGGCACAGGGATCAA
CAATGACATCAAACCATGGAAGCTGGTGGCGTGATAAAATCCATGGAAGACTGTCTGGTCCTCC
AAAGAACAAGGCCTGAAAGACGAGCGAAACTTATGAAAATAGAATATATGAGACATGTAGTTCG
AATATTGATCAAATGCCATAGAACTAGATGAAGGAAAAACGACACTTACAAGATTGGGGGATAT
GCCACGGGCAACAGCAGATCCACCAATAGTATATTCCAGCACTAAAGCCCAACCAATCAACCAG
GCAACA
						
					

			
		

Sequences
Splice junction sequence	AAAAACGACACTTACAAGATTGGGGGATATGCCACGGGCAACAGCAGATCCACCAATAGTATAT TCCAGCACTAAAGCCCAACCAATCAACCAGGCAACAcattcctgccagctgggaaacatccatg aaaaaagccagagctgctgcacagagaccagtcacgactgtgctctttacaggaacttgtgtcc tcttgtta
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CATTCCTGCCAGCTGGGAAACATCCATGAAAAAAGCCAGAGCTGCTGCACAGAGACCAGTCACG ACTGTGCTCTTTACAGGAACTTGTGTCCTCTTGTTAACATCGGCGAAGAAAGATGGCAGCAGCC CGTCTCGTGCCATCGCCATTAGTATTCTAGGCTGCATGAAATGGCAACTAAAATCTTTAAAATG TATTTTAAAAAACAAGGTAATGCATCCCCAAGTTCTCAGTTTCACATAATAATGTGCAAGGAAA GAGTGGGCGGGTGGGTGTCTGTTTCTAAGTTTATGTTTTTGTGTTCTCATTTGAGTTTGTCTGT GTAGGCATGGGATCAGTTTTACGTATAAGGCAATCAGGGATAGCTCAAATGTAACAAGTACTGG TATACATGCCACATCATCAATCTAGTGTTGATGTGTGTGCTACAAATTTCATTTATGTTGATAT GGTATTAGTTCAATATACATTTGCAGGTACTATAGTTTATTTTGTCAAAACAGAGCCTATCGGC AAAATTGCAAGGAGAGAAACAACGTTAATCATTATATGAATAGTTTAATTAAAATTCAAAAATC AGAATGCAAGTGAAAAGTGCAATCAACTGCTCGCTAATGTACTGCAGAATAATTGACCTTATGG ACCAAGCAGTTTGATTCAGATTCAGAAATCAGAATACAAGTGAGAAGTACAACAAAAAAATAAC TTTTTTTCCACAAGGATTATAGATTCAGCTTTGGCTAGTTAGAAGACCACTCTCACTAATATAA TGCGGAATAATTGACTGTGATATTAGAACGCAAATTCCTTATGGACCAAGCATATAGTAGACGA CTAGAATCAACTCTATGTGCTCACTGTCTTGTTTAAAGCTTGGGAATTATTAGGATTTTTTTTT ATATCGAGGGATGTGTTTGTATACCTGCGGAAGAAGTGACCCCAACAAAGTTGAACAAAGTGCA AGAACAGCACCACTTGTCACAATATACCTAAAACAAAACAGCATAGTGACAGACTAAATAAGCA CGTTGCAAGACCATTATAGATAATTGGATATATGGGAATAAACAAAAGGAGAGCGAGTCCTTAC ATGGCCCACTGCATTCCATGTTTCGCAAAGACAGATGAAATGGGCGTATCTGGGTCCATAGCGA AATATGGTACCAGCCCAACAATAACAACAGAAACCATCATGTACAAGCAACAGCAGATAGACAA TGCCGCTCCTATTCCCAGTGGTAGATCTCTCTGTGGATTCTTCACCTATAAGGGTTGAAAGAGG GAAAAATCAGCATCCCCATACAGGAAGTGGACGACATGGTTAGTTAGGATCATCTTAACCTCCT CAGCAGTACTAGCAACTGTATCAAAGCCAATATATGCAAAGAAAACAGTTGCTGATCCAGCAAG CATTCCATTTATCCCATGTGGAAAATATCTGAAATGTTCAAATGGTAAGTGCATCAGTAATTAC TCTCAAAATACAATCAACTGTTTTTTTTTTTTTGCATTACCCATCAGTAACTTTATAGCCAACC CATCCTATCTGGAAGCCGATATAACTACCAGCTACAATAACAAATATCATCACACAAGCATTCA GAACTGTTATAAGCTCTTGTACAGCTGAACTCTGCAACAGATTCAAATAGAATAATTAGTAACA GTCCTATAATCCTAGTTTGCAGATGAAAGAATAGGAAACTTACAAATCGACCAATGTAACAACC TCTTTTATCCCAACACATAGCAAAACAGTAACAACAAAAACGAGGGCAGCGGCACAGGGATCAA CAATGACATCAAACCATGGAAGCTGGTGGCGTGATAAAATCCATGGAAGACTGTCTGGTCCTCC AAAGAACAAGGCCTGAAAGACGAGCGAAACTTATGAAAATAGAATATATGAGACATGTAGTTCG AATATTGATCAAATGCCATAGAACTAGATGAAGGAAAAACGACACTTACAAGATTGGGGGATAT GCCACGGGCAACAGCAGATCCACCAATAGTATATTCCAGCACTAAAGCCCAACCAATCAACCAG GCAACA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.156427171

Conservation Information
Conserved circRNAs	NA
PMCS	0.156427171

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							25507780-25506131(+)
25507572-25507787(-)
25506009-25507787(-)
						
						Potential amino acid sequence
						
							MTSNHGSWWRDKIHGRLSGPPKNKARLGDMPRATADPPIVYSSTKAQPINQATHSCQLGNIHEK
SQSCCTETSHDCALYRNLCPLVNIGEERWQQPVSCHRH*(+)
MIFVIVAGSYIGFQIGWVGYKVTDGYFPHGINGMLAGSATVFFAYIGFDTVASTAEEVKNPQRD
LPLGIGAALSICCCLYMMVSVVIVGLVPYFAMDPDTPISSVFAKHGMQWAMYIVTSGAVLALCS
TLLGSLLPQPRILMAMARDGLLPSFFADVNKRTQVPVKSTVVTGLCAAALAFFMDVSQLAGMCC
LVDWLGFSAGIYYWWICCCPWHIPQSCLVLWRTRQSSMDFITPPASMV*(-)
MFPSWQECVAWLIGWALVLEYTIGGSAVARGISPNLALFFGGPDSLPWILSRHQLPWFDVIVDP
CAAALVFVVTVLLCVGIKESSAVQELITVLNACVMIFVIVAGSYIGFQIGWVGYKVTDGYFPHG
INGMLAGSATVFFAYIGFDTVASTAEEVKNPQRDLPLGIGAALSICCCLYMMVSVVIVGLVPYF
AMDPDTPISSVFAKHGMQWAMYIVTSGAVLALCSTLLGSLLPQPRILMAMARDGLLPSFFADVN
KRTQVPVKSTVVTGLCAAALAFFMDVSQLAGMCCLVDWLGFSAGIYYWWICCCPWHIPQSCLVL
WRTRQSSMDFITPPASMV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	25507780-25506131(+) 25507572-25507787(-) 25506009-25507787(-)
Potential amino acid sequence	MTSNHGSWWRDKIHGRLSGPPKNKARLGDMPRATADPPIVYSSTKAQPINQATHSCQLGNIHEK SQSCCTETSHDCALYRNLCPLVNIGEERWQQPVSCHRH(+) MIFVIVAGSYIGFQIGWVGYKVTDGYFPHGINGMLAGSATVFFAYIGFDTVASTAEEVKNPQRD LPLGIGAALSICCCLYMMVSVVIVGLVPYFAMDPDTPISSVFAKHGMQWAMYIVTSGAVLALCS TLLGSLLPQPRILMAMARDGLLPSFFADVNKRTQVPVKSTVVTGLCAAALAFFMDVSQLAGMCC LVDWLGFSAGIYYWWICCCPWHIPQSCLVLWRTRQSSMDFITPPASMV(-) MFPSWQECVAWLIGWALVLEYTIGGSAVARGISPNLALFFGGPDSLPWILSRHQLPWFDVIVDP CAAALVFVVTVLLCVGIKESSAVQELITVLNACVMIFVIVAGSYIGFQIGWVGYKVTDGYFPHG INGMLAGSATVFFAYIGFDTVASTAEEVKNPQRDLPLGIGAALSICCCLYMMVSVVIVGLVPYF AMDPDTPISSVFAKHGMQWAMYIVTSGAVLALCSTLLGSLLPQPRILMAMARDGLLPSFFADVN KRTQVPVKSTVVTGLCAAALAFFMDVSQLAGMCCLVDWLGFSAGIYYWWICCCPWHIPQSCLVL WRTRQSSMDFITPPASMV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os03g0654400_circ_g.2

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