Plant Circular RNA Database

Detail information of Os02g0255033_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0255033_circ_g.2
					
						ID in PlantcircBase
						osa_circ_014125
					
						Alias
						Os02circ09599/Os_ciR2518
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 8750315-8751439  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ui-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os02g0255033						
					
						Parent gene annotation
						
							Hypothetical protein. (Os02t0255033-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						4/8/3
					
						Tissues
						leaf and panicle/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0255000-02:3
Os02t0255000-03:2
Os02t0255000-01:2
Os02t0255000-02:3
Os02t0255033-00:2
Os02t0255000-03:2
Os02t0255000-01:2

General Information
CircRNA Name	Os02g0255033_circ_g.2
ID in PlantcircBase	osa_circ_014125
Alias	Os02circ09599/Os_ciR2518
Organism	Oryza sativa
Position	chr2: 8750315-8751439 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ui-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os02g0255033
Parent gene annotation	Hypothetical protein. (Os02t0255033-00)
Parent gene strand	+
Alternative splicing	NA
Support reads	4/8/3
Tissues	leaf and panicle/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0255000-02:3 Os02t0255000-03:2 Os02t0255000-01:2 Os02t0255000-02:3 Os02t0255033-00:2 Os02t0255000-03:2 Os02t0255000-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTCG
TTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGACcctcatgagaagcgacaggcaaagatgc
ctctttatctctctcctaagattcttaggaagatttgaaagaaggccctcttcatcaacccctc
ttgtttct

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 293
Transcript exons: 8750315-8750383,8750387-8750487,8751317-8751439
CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT
GAAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTATTC
GCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTTCTAGCTGCATTACTCCCACAA
AATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTC
GTTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGAC
Transcript length: 715
Transcript exons: 8750315-8750551,8750962-8751439
CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT
GAAAGAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTA
TTCGCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTGCTCTGTGTCACGCCGTTT
CACTCTCATTTCTTCTATACGCACAGAGGCAGATTGTAGATAGGTCTGCACATTACCAATAAGG
AGTGCAAACAAAACTAGACCTGATGTCGAGACGAAAACAGCAAATAGATTCTCCCATGTATACG
TGCTTGTCTTAAGATTTTGACCAAGAGAACTGCAACAAATAAAAAACTATATAGAAACAATATT
GAAGTATGTGTTCTTCAAGGGAAAATAAAACGAAGGTTATTTCTACAGACCTTAGATTTTGCAG
ACCCCACCAAAAGCAATAAAACAATTTTTCAAAGAAACTCGTTGATTGTGAAACATTTTGAAGA
GCTGGAAGATAAATTCCGAAAGTTGGATCTGCATTGTCAGTTCCATTTGTCGGGCAAAAATCTT
GTAAGAAACTGTTATTTCTAGCTGCATTACTCCCACAAAATAAAGATCCCGAATCACAGCCATC
ATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTCGTTGAATGGAAAGAAGGTACCAAAGA
GCTCCAAGGAC
						
					

						Genomic sequence
						
							CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT
GAAAGAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTA
TTCGCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTGCTCTGTGTCACGCCGTTT
CACTCTCATTTCTTCTATACGCACAGAGGCAGATTGTAGATAGGTCTGCAAAATACATGCAATA
GAAAATTACGAAAGGAAGAAATCGAGCCGCTTACACATTTAATTGTCATCCCGATTACACCTTA
AACAACAGAGTTGTTTTCATTACATGTAATATAAGTAAATAGCATTGCAAAAAAGGAGCTGTTT
AACCCTTTAATTTTTAAGGAGAAAAAATGATACATAAAGAGAATATGAATTGGCTATATAACTC
GTTAATTGTGGCTTCTATTGAAAAGACAATATGCATGCCAGAGAATAGATAAAGAGAACATGCA
TGTCAGAGAAACACATCTACAGTGTACTATTTTCATTAAAAAAGATACTCCACACAAGTTGCAT
ATATAAAAAATATTAACTACTTAATCCAATTATGATCATAATAAAAATATGACAGTTGATATAT
TCCATACCTGCACATTACCAATAAGGAGTGCAAACAAAACTAGACCTGATGTCGAGACGAAAAC
AGCAAATAGATTCTCCCATGTATACGTGCTTGTCTTAAGATTTTGACCAAGAGAACTGCAACAA
ATAAAAAACTATATAGAAACAATATTGAAGTATGTGTTCTTCAAGGGAAAATAAAACGAAGGTT
ATTTCTACAGACCTTAGATTTTGCAGACCCCACCAAAAGCAATAAAACAATTTTTCAAAGAAAC
TCGTTGATTGTGAAACATTTTGAAGAGCTGGAAGATAAATTCCGAAAGTTGGATCTGCATTGTC
AGTTCCATTTGTCGGGCAAAAATCTTGTAAGAAACTGTTATTTCTAGCTGCATTACTCCCACAA
AATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTC
GTTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGAC
						
					

			
		

Sequences
Splice junction sequence	ATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTCG TTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGACcctcatgagaagcgacaggcaaagatgc ctctttatctctctcctaagattcttaggaagatttgaaagaaggccctcttcatcaacccctc ttgtttct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 293 Transcript exons: 8750315-8750383,8750387-8750487,8751317-8751439 CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT GAAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTATTC GCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTTCTAGCTGCATTACTCCCACAA AATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTC GTTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGAC Transcript length: 715 Transcript exons: 8750315-8750551,8750962-8751439 CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT GAAAGAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTA TTCGCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTGCTCTGTGTCACGCCGTTT CACTCTCATTTCTTCTATACGCACAGAGGCAGATTGTAGATAGGTCTGCACATTACCAATAAGG AGTGCAAACAAAACTAGACCTGATGTCGAGACGAAAACAGCAAATAGATTCTCCCATGTATACG TGCTTGTCTTAAGATTTTGACCAAGAGAACTGCAACAAATAAAAAACTATATAGAAACAATATT GAAGTATGTGTTCTTCAAGGGAAAATAAAACGAAGGTTATTTCTACAGACCTTAGATTTTGCAG ACCCCACCAAAAGCAATAAAACAATTTTTCAAAGAAACTCGTTGATTGTGAAACATTTTGAAGA GCTGGAAGATAAATTCCGAAAGTTGGATCTGCATTGTCAGTTCCATTTGTCGGGCAAAAATCTT GTAAGAAACTGTTATTTCTAGCTGCATTACTCCCACAAAATAAAGATCCCGAATCACAGCCATC ATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTCGTTGAATGGAAAGAAGGTACCAAAGA GCTCCAAGGAC
Genomic sequence	CCTCATGAGAAGCGACAGGCAAAGATGCCTCTTTATCTCTCTCCTAAGATTCTTAGGAAGATTT GAAAGAAGGCCCTCTTCATCAACCCCTCTTGTTTCTTGCCACCTATATTGTTCATGACGCAGTA TTCGCTCCTTGAGGTTATCAGGAAGTAATCGATGTGCCATCCATTGCTCTGTGTCACGCCGTTT CACTCTCATTTCTTCTATACGCACAGAGGCAGATTGTAGATAGGTCTGCAAAATACATGCAATA GAAAATTACGAAAGGAAGAAATCGAGCCGCTTACACATTTAATTGTCATCCCGATTACACCTTA AACAACAGAGTTGTTTTCATTACATGTAATATAAGTAAATAGCATTGCAAAAAAGGAGCTGTTT AACCCTTTAATTTTTAAGGAGAAAAAATGATACATAAAGAGAATATGAATTGGCTATATAACTC GTTAATTGTGGCTTCTATTGAAAAGACAATATGCATGCCAGAGAATAGATAAAGAGAACATGCA TGTCAGAGAAACACATCTACAGTGTACTATTTTCATTAAAAAAGATACTCCACACAAGTTGCAT ATATAAAAAATATTAACTACTTAATCCAATTATGATCATAATAAAAATATGACAGTTGATATAT TCCATACCTGCACATTACCAATAAGGAGTGCAAACAAAACTAGACCTGATGTCGAGACGAAAAC AGCAAATAGATTCTCCCATGTATACGTGCTTGTCTTAAGATTTTGACCAAGAGAACTGCAACAA ATAAAAAACTATATAGAAACAATATTGAAGTATGTGTTCTTCAAGGGAAAATAAAACGAAGGTT ATTTCTACAGACCTTAGATTTTGCAGACCCCACCAAAAGCAATAAAACAATTTTTCAAAGAAAC TCGTTGATTGTGAAACATTTTGAAGAGCTGGAAGATAAATTCCGAAAGTTGGATCTGCATTGTC AGTTCCATTTGTCGGGCAAAAATCTTGTAAGAAACTGTTATTTCTAGCTGCATTACTCCCACAA AATAAAGATCCCGAATCACAGCCATCATGTCTACTACATGCATCTTTCCAACAGGTGTCTTCTC GTTGAATGGAAAGAAGGTACCAAAGAGCTCCAAGGAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_003801* osi_circ_012796 						
					
						PMCS
						0.392510504

Conservation Information
Conserved circRNAs	osi_circ_003801* osi_circ_012796
PMCS	0.392510504

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8751377-8750319(+)
8751366-8750377(+)
8751409-8750432(+)
8750517-8751055(-)
						
						Potential amino acid sequence
						
							MHLSNRCLLVEWKEGTKELQGPS*(+)
MSTTCIFPTGVFSLNGKKVPKSSKDPHEKRQAKMPLYLSPKILRKI*(+)
MERRYQRAPRTLMRSDRQRCLFISLLRFLGRFERRPSSSTPLVSCHLYCS*(+)
MRVKRRDTEQWMAHRLLPDNLKERILRHEQYRWQETRGVDEEGLLSNLPKNLRREIKRHLCLSL
LMRVLGALWYLLSIQREDTCWKDACSRHDGCDSGSLFCGSNAARNNSFLQDFCPTNGTDNADPT
FGIYLPALQNVSQSTSFFEKLFYCFWWGLQNLRSVEITFVLFSLEEHILQYCFYIVFYLLQFSW
SKS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8751377-8750319(+) 8751366-8750377(+) 8751409-8750432(+) 8750517-8751055(-)
Potential amino acid sequence	MHLSNRCLLVEWKEGTKELQGPS(+) MSTTCIFPTGVFSLNGKKVPKSSKDPHEKRQAKMPLYLSPKILRKI(+) MERRYQRAPRTLMRSDRQRCLFISLLRFLGRFERRPSSSTPLVSCHLYCS(+) MRVKRRDTEQWMAHRLLPDNLKERILRHEQYRWQETRGVDEEGLLSNLPKNLRREIKRHLCLSL LMRVLGALWYLLSIQREDTCWKDACSRHDGCDSGSLFCGSNAARNNSFLQDFCPTNGTDNADPT FGIYLPALQNVSQSTSFFEKLFYCFWWGLQNLRSVEITFVLFSLEEHILQYCFYIVFYLLQFSW SKS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0255033_circ_g.2

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