Plant Circular RNA Database

Detail information of Os02g0775400_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os02g0775400_circ_g.4
					
						ID in PlantcircBase
						osa_circ_016732
					
						Alias
						Os_ciR4287
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 32734257-32736037  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os02g0775400						
					
						Parent gene annotation
						
							Similar to Kinesin heavy chain-like protein (Fragment). (Os02t07
75400-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0775400_circ_g.2 Os02g0775400_circ_g.3 Os02g0775400_circ_g.5 Os02g0775400_circ_g.6 						
					
						Support reads
						6
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0775400-01:6

General Information
CircRNA Name	Os02g0775400_circ_g.4
ID in PlantcircBase	osa_circ_016732
Alias	Os_ciR4287
Organism	Oryza sativa
Position	chr2: 32734257-32736037 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	Os02g0775400
Parent gene annotation	Similar to Kinesin heavy chain-like protein (Fragment). (Os02t07 75400-01)
Parent gene strand	-
Alternative splicing	Os02g0775400_circ_g.2 Os02g0775400_circ_g.3 Os02g0775400_circ_g.5 Os02g0775400_circ_g.6
Support reads	6
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0775400-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CACGTCCTTGACCACTCAAGGATGACTGAAGTAGACGTGTTAGTTTTGAATCACGAAATGGAAT
ATGTGTAGCTTTTCCGTCAGTAAGTTTCGATATCACcagaggtgtttcttctccatgaaggctg
tcagtagcgaactccccatggccaatgctaaagagatcaaccgatgcactctcaccaaccaaag
aatttgat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 914
Transcript exons: 32734257-32734478,32734667-32734819,32735043-32735221,32
735471-32735617,32735719-32735826,32735933-32736037
CAGAGGTGTTTCTTCTCCATGAAGGCTGTCAGTAGCGAACTCCCCATGGCCAATGCTAAAGAGA
TCAACCGATGCACTCTCACCAACCAAAGAATTTGATATTCTTGGTTCGGAAGGAAAATTAACAC
TCTCCCCAATAGGTGTTGAAGGAGCAGTTGATTTAGTCAAACTGGACTTGTCACCTTCTGAACT
TGTCAGAATAGAAGCTCCACCTTCACGTTTCCGAAGTTTAAACCAATTAAGAATTCCTTTCCGA
TTCTTTTTCTCCTCCTTCGAATCTTCAAGTGTCATGCCAAGTCCTTCAACAGGAGAAAGTAATT
CATTGCTTTCATTATCCAACACGATATCACGTCTTTTGTAAGGTAGGTACGCAAGCTCCTCTTC
CCCAAAAGAATGTCTTCTTCTTGGTCCAGGATGTGGAGAAAATCTAGAAGTTTGTGTCGCCTTA
GTGGAGACCAAGATAAGCTTTGTGAGACGCTGTATCCTTGCAAGCAAAGCAGCTTTAGCTTCCT
CCTCTTGTTCGAGCCTAGACTGCAGCTTGACGTTACCATCTTCTTTTGTTTCCAAAGGATGATA
TTATCTTCTCCGGCATCCTTCACTGGAGTGCCAGTAATAATACCCATTTTCAGCTGTTCTAGTT
CTTCCTTTAATCTGCGAATCTCATTCTGGTATTTCTTTATTAAAGATTTTTCATCTATAATTTT
GTTTTGTGATGCTTGGACCTCAATGCGTTTTGCACGGTGGGCAAATTTCAGTGTATTATGAGTC
TCTTCTGAGTTGCTCGATGCTGGAGTCACTGTACAGATCAGTGAAACACGTCCTTGACCACTCA
AGGATGACTGAAGTAGACGTGTTAGTTTTGAATCACGAAATGGAATATGTGTAGCTTTTCCGTC
AGTAAGTTTCGATATCAC
						
					

						Genomic sequence
						
							CAGAGGTGTTTCTTCTCCATGAAGGCTGTCAGTAGCGAACTCCCCATGGCCAATGCTAAAGAGA
TCAACCGATGCACTCTCACCAACCAAAGAATTTGATATTCTTGGTTCGGAAGGAAAATTAACAC
TCTCCCCAATAGGTGTTGAAGGAGCAGTTGATTTAGTCAAACTGGACTTGTCACCTTCTGAACT
TGTCAGAATAGAAGCTCCACCTTCACGTTTCTGGACAATAGAACCATGGGAAAATGTTCGTGTG
AATCAATTATTATTTCAATTTTAAAATCAGGATTGGTGCACATGAGTTCATCTATATTGTAACA
ATTCATGACGATTGCACTTGCATCTATGTAAAAGATAAATCAAAAGAATTGAATAAATGGACTA
TTAAGAATTGCCCAATTTAACTATACCCGAAGTTTAAACCAATTAAGAATTCCTTTCCGATTCT
TTTTCTCCTCCTTCGAATCTTCAAGTGTCATGCCAAGTCCTTCAACAGGAGAAAGTAATTCATT
GCTTTCATTATCCAACACGATATCACGTCTTTTGTAAGGTAGGTACGCAAGCTGCAATTTGAAC
AAGCAAACTTTATCATACAGGAAGTTACCCATACTAACTATAATGAGGAAAAAAATAAAGTAGT
AACTCAAATTTTCTGCAAACACACTAAAACAGAGTCTGCCGATTTATTGCCAAAAGTATCTCAG
TAACAAAAGCAAACTACATTATCGAAAAAACAAAAGCAAACTAAGGTCAAAGTGTAACATGTGT
TTGACATCATAACCATACCTCCTCTTCCCCAAAAGAATGTCTTCTTCTTGGTCCAGGATGTGGA
GAAAATCTAGAAGTTTGTGTCGCCTTAGTGGAGACCAAGATAAGCTTTGTGAGACGCTGTATCC
TTGCAAGCAAAGCAGCTTTAGCTTCCTCCTCTTGTTCGAGCCTAGACTGCAGCTTGACGTTACC
ATCTTCTAGCTTTTCATATGATAAACAAACTCCAGTTAGGATGATACATTCATACACCGGCAAA
GTGAACATGCAACATTATATAACTTGTAAGTATAATATTATTAATCTACAGCACAGTTCAAGCA
GACAGGAGTAGCTGAAGACAAATCTGTAAACAACTCAGTTAATAGCATAGCAGATCTCGCATAC
ATACAGTTAAGCATAATGTGAGCAGCAAAGGGGCAATTGCTATAAGACAATAAATATTCCACCT
TTTGTTTCCAAAGGATGATATTATCTTCTCCGGCATCCTTCACTGGAGTGCCAGTAATAATACC
CATTTTCAGCTGTTCTAGTTCTTCCTTTAATCTGCGAATCTCATTCTGGTATTTCTTTATTAAA
GATTTTTCATCTATAATCTGCCCAACATAAAGAAAGATTCAGGAGAATATGTCAGAAATACTTC
ATATGATATTTGGCACAAAAATAATGGTTAAGAATTCCTGCTAACATAACCTACTTTGTTTTGT
GATGCTTGGACCTCAATGCGTTTTGCACGGTGGGCAAATTTCAGTGTATTATGAGTCTCTTCTG
AGTTGCTCGATGCTGGAGTCACTGTACAGATCAGCTGCAAAGTATAATAGAATGAAGTTGTCAG
ACATCCAAATCACATTGAATATTAAAAGAAATACATTAGCTTTAACCACCATCCCAACTGATAA
TGAACAACTTACTGAAACACGTCCTTGACCACTCAAGGATGACTGAAGTAGACGTGTTAGTTTT
GAATCACGAAATGGAATATGTGTAGCTTTTCCGTCAGTAAGTTTCGATATCAC
						
					

			
		

Sequences
Splice junction sequence	CACGTCCTTGACCACTCAAGGATGACTGAAGTAGACGTGTTAGTTTTGAATCACGAAATGGAAT ATGTGTAGCTTTTCCGTCAGTAAGTTTCGATATCACcagaggtgtttcttctccatgaaggctg tcagtagcgaactccccatggccaatgctaaagagatcaaccgatgcactctcaccaaccaaag aatttgat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 914 Transcript exons: 32734257-32734478,32734667-32734819,32735043-32735221,32 735471-32735617,32735719-32735826,32735933-32736037 CAGAGGTGTTTCTTCTCCATGAAGGCTGTCAGTAGCGAACTCCCCATGGCCAATGCTAAAGAGA TCAACCGATGCACTCTCACCAACCAAAGAATTTGATATTCTTGGTTCGGAAGGAAAATTAACAC TCTCCCCAATAGGTGTTGAAGGAGCAGTTGATTTAGTCAAACTGGACTTGTCACCTTCTGAACT TGTCAGAATAGAAGCTCCACCTTCACGTTTCCGAAGTTTAAACCAATTAAGAATTCCTTTCCGA TTCTTTTTCTCCTCCTTCGAATCTTCAAGTGTCATGCCAAGTCCTTCAACAGGAGAAAGTAATT CATTGCTTTCATTATCCAACACGATATCACGTCTTTTGTAAGGTAGGTACGCAAGCTCCTCTTC CCCAAAAGAATGTCTTCTTCTTGGTCCAGGATGTGGAGAAAATCTAGAAGTTTGTGTCGCCTTA GTGGAGACCAAGATAAGCTTTGTGAGACGCTGTATCCTTGCAAGCAAAGCAGCTTTAGCTTCCT CCTCTTGTTCGAGCCTAGACTGCAGCTTGACGTTACCATCTTCTTTTGTTTCCAAAGGATGATA TTATCTTCTCCGGCATCCTTCACTGGAGTGCCAGTAATAATACCCATTTTCAGCTGTTCTAGTT CTTCCTTTAATCTGCGAATCTCATTCTGGTATTTCTTTATTAAAGATTTTTCATCTATAATTTT GTTTTGTGATGCTTGGACCTCAATGCGTTTTGCACGGTGGGCAAATTTCAGTGTATTATGAGTC TCTTCTGAGTTGCTCGATGCTGGAGTCACTGTACAGATCAGTGAAACACGTCCTTGACCACTCA AGGATGACTGAAGTAGACGTGTTAGTTTTGAATCACGAAATGGAATATGTGTAGCTTTTCCGTC AGTAAGTTTCGATATCAC
Genomic sequence	CAGAGGTGTTTCTTCTCCATGAAGGCTGTCAGTAGCGAACTCCCCATGGCCAATGCTAAAGAGA TCAACCGATGCACTCTCACCAACCAAAGAATTTGATATTCTTGGTTCGGAAGGAAAATTAACAC TCTCCCCAATAGGTGTTGAAGGAGCAGTTGATTTAGTCAAACTGGACTTGTCACCTTCTGAACT TGTCAGAATAGAAGCTCCACCTTCACGTTTCTGGACAATAGAACCATGGGAAAATGTTCGTGTG AATCAATTATTATTTCAATTTTAAAATCAGGATTGGTGCACATGAGTTCATCTATATTGTAACA ATTCATGACGATTGCACTTGCATCTATGTAAAAGATAAATCAAAAGAATTGAATAAATGGACTA TTAAGAATTGCCCAATTTAACTATACCCGAAGTTTAAACCAATTAAGAATTCCTTTCCGATTCT TTTTCTCCTCCTTCGAATCTTCAAGTGTCATGCCAAGTCCTTCAACAGGAGAAAGTAATTCATT GCTTTCATTATCCAACACGATATCACGTCTTTTGTAAGGTAGGTACGCAAGCTGCAATTTGAAC AAGCAAACTTTATCATACAGGAAGTTACCCATACTAACTATAATGAGGAAAAAAATAAAGTAGT AACTCAAATTTTCTGCAAACACACTAAAACAGAGTCTGCCGATTTATTGCCAAAAGTATCTCAG TAACAAAAGCAAACTACATTATCGAAAAAACAAAAGCAAACTAAGGTCAAAGTGTAACATGTGT TTGACATCATAACCATACCTCCTCTTCCCCAAAAGAATGTCTTCTTCTTGGTCCAGGATGTGGA GAAAATCTAGAAGTTTGTGTCGCCTTAGTGGAGACCAAGATAAGCTTTGTGAGACGCTGTATCC TTGCAAGCAAAGCAGCTTTAGCTTCCTCCTCTTGTTCGAGCCTAGACTGCAGCTTGACGTTACC ATCTTCTAGCTTTTCATATGATAAACAAACTCCAGTTAGGATGATACATTCATACACCGGCAAA GTGAACATGCAACATTATATAACTTGTAAGTATAATATTATTAATCTACAGCACAGTTCAAGCA GACAGGAGTAGCTGAAGACAAATCTGTAAACAACTCAGTTAATAGCATAGCAGATCTCGCATAC ATACAGTTAAGCATAATGTGAGCAGCAAAGGGGCAATTGCTATAAGACAATAAATATTCCACCT TTTGTTTCCAAAGGATGATATTATCTTCTCCGGCATCCTTCACTGGAGTGCCAGTAATAATACC CATTTTCAGCTGTTCTAGTTCTTCCTTTAATCTGCGAATCTCATTCTGGTATTTCTTTATTAAA GATTTTTCATCTATAATCTGCCCAACATAAAGAAAGATTCAGGAGAATATGTCAGAAATACTTC ATATGATATTTGGCACAAAAATAATGGTTAAGAATTCCTGCTAACATAACCTACTTTGTTTTGT GATGCTTGGACCTCAATGCGTTTTGCACGGTGGGCAAATTTCAGTGTATTATGAGTCTCTTCTG AGTTGCTCGATGCTGGAGTCACTGTACAGATCAGCTGCAAAGTATAATAGAATGAAGTTGTCAG ACATCCAAATCACATTGAATATTAAAAGAAATACATTAGCTTTAACCACCATCCCAACTGATAA TGAACAACTTACTGAAACACGTCCTTGACCACTCAAGGATGACTGAAGTAGACGTGTTAGTTTT GAATCACGAAATGGAATATGTGTAGCTTTTCCGTCAGTAAGTTTCGATATCAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.2566352

Conservation Information
Conserved circRNAs	NA
PMCS	0.2566352

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32736003-32734352(+)
32734737-32734734(-)
						
						Potential amino acid sequence
						
							MCSFSVSKFRYHQRCFFSMKAVSSELPMANAKEINRCTLTNQRI*(+)
MTLEDSKEEKKNRKGILNWFKLRKREGGASILTSSEGDKSSLTKSTAPSTPIGESVNFPSEPRI
SNSLVGESASVDLFSIGHGEFATDSLHGEETPLVISKLTDGKATHIPFRDSKLTRLLQSSLSGQ
GRVSLICTVTPASSNSEETHNTLKFAHRAKRIEVQASQNKIIDEKSLIKKYQNEIRRLKEELEQ
LKMGIITGTPVKDAGEDNIILWKQKKMVTSSCSLGSNKRRKLKLLCLQGYSVSQSLSWSPLRRH
KLLDFLHILDQEEDILLGKRSLRTYLTKDVISCWIMKAMNYFLLLKDLA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32736003-32734352(+) 32734737-32734734(-)
Potential amino acid sequence	MCSFSVSKFRYHQRCFFSMKAVSSELPMANAKEINRCTLTNQRI(+) MTLEDSKEEKKNRKGILNWFKLRKREGGASILTSSEGDKSSLTKSTAPSTPIGESVNFPSEPRI SNSLVGESASVDLFSIGHGEFATDSLHGEETPLVISKLTDGKATHIPFRDSKLTRLLQSSLSGQ GRVSLICTVTPASSNSEETHNTLKFAHRAKRIEVQASQNKIIDEKSLIKKYQNEIRRLKEELEQ LKMGIITGTPVKDAGEDNIILWKQKKMVTSSCSLGSNKRRKLKLLCLQGYSVSQSLSWSPLRRH KLLDFLHILDQEEDILLGKRSLRTYLTKDVISCWIMKAMNYFLLLKDLA(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os02g0775400_circ_g.4

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