Plant Circular RNA Database

Detail information of Os07g0205200_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os07g0205200_circ_g.2
					
						ID in PlantcircBase
						osa_circ_033161
					
						Alias
						Os07circ04681
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 5661519-5661830  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os07g0205200						
					
						Parent gene annotation
						
							Hypothetical conserved gene. (Os07t0205200-01);Similar to TAF5 (
TBP-ASSOCIATED FACTOR 5); nucleotide binding / transcription reg
ulator. (Os07t0205200-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os07g0205200_circ_g.1 Os07g0205200_circ_g.3 						
					
						Support reads
						3
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0205200-01:2
Os07t0205200-02:2

General Information
CircRNA Name	Os07g0205200_circ_g.2
ID in PlantcircBase	osa_circ_033161
Alias	Os07circ04681
Organism	Oryza sativa
Position	chr7: 5661519-5661830 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os07g0205200
Parent gene annotation	Hypothetical conserved gene. (Os07t0205200-01);Similar to TAF5 ( TBP-ASSOCIATED FACTOR 5); nucleotide binding / transcription reg ulator. (Os07t0205200-02)
Parent gene strand	+
Alternative splicing	Os07g0205200_circ_g.1 Os07g0205200_circ_g.3
Support reads	3
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0205200-01:2 Os07t0205200-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTTTTATTGCAGTATTCATATGAATTGCTTCTCCAGTATCTCCACAAAACACAAGCTCTTGTGG
TGCTTGGAATAATCAATGAACGCATAGATTTTCAAGcaaatggaagtggctcgatctttaaggc
agaataaattcagaattaagctatgtgaggttgtataccatttccaaatcccctttcatttcaa
ccctgtac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 145
Transcript exons: 5661519-5661575,5661743-5661830
CAAATGGAAGTGGCTCGATCTTTAAGGCAGAATAAATTCAGAATTAAGCTATGTGAGTATTCAT
ATGAATTGCTTCTCCAGTATCTCCACAAAACACAAGCTCTTGTGGTGCTTGGAATAATCAATGA
ACGCATAGATTTTCAAG
						
						Genomic sequence
						
							CAAATGGAAGTGGCTCGATCTTTAAGGCAGAATAAATTCAGAATTAAGCTATGTGAGGTTGTAT
ACCATTTCCAAATCCCCTTTCATTTCAACCCTGTACCATGTACTATTTTTTGGGGCAGCATTTC
ATGATAGCCACTGTAATTTCTTGATTGCTGCAAAATTGCTTTTCTGGAAATATAATCAGCTGTC
CCAATTAAAGTTTCTGTCTTTTTTTATTGCAGTATTCATATGAATTGCTTCTCCAGTATCTCCA
CAAAACACAAGCTCTTGTGGTGCTTGGAATAATCAATGAACGCATAGATTTTCAAG

Sequences
Splice junction sequence	TTTTTATTGCAGTATTCATATGAATTGCTTCTCCAGTATCTCCACAAAACACAAGCTCTTGTGG TGCTTGGAATAATCAATGAACGCATAGATTTTCAAGcaaatggaagtggctcgatctttaaggc agaataaattcagaattaagctatgtgaggttgtataccatttccaaatcccctttcatttcaa ccctgtac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 145 Transcript exons: 5661519-5661575,5661743-5661830 CAAATGGAAGTGGCTCGATCTTTAAGGCAGAATAAATTCAGAATTAAGCTATGTGAGTATTCAT ATGAATTGCTTCTCCAGTATCTCCACAAAACACAAGCTCTTGTGGTGCTTGGAATAATCAATGA ACGCATAGATTTTCAAG
Genomic sequence	CAAATGGAAGTGGCTCGATCTTTAAGGCAGAATAAATTCAGAATTAAGCTATGTGAGGTTGTAT ACCATTTCCAAATCCCCTTTCATTTCAACCCTGTACCATGTACTATTTTTTGGGGCAGCATTTC ATGATAGCCACTGTAATTTCTTGATTGCTGCAAAATTGCTTTTCTGGAAATATAATCAGCTGTC CCAATTAAAGTTTCTGTCTTTTTTTATTGCAGTATTCATATGAATTGCTTCTCCAGTATCTCCA CAAAACACAAGCTCTTGTGGTGCTTGGAATAATCAATGAACGCATAGATTTTCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.201217067

Conservation Information
Conserved circRNAs	NA
PMCS	0.201217067

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5661523-5661550(+)
5661821-5661748(-)
						
						Potential amino acid sequence
						
							MEVARSLRQNKFRIKLCEYSYELLLQYLHKTQALVVLGIINERIDFQANGSGSIFKAE*(+)
MRSLIIPSTTRACVLWRYWRSNSYEYSHSLILNLFCLKDRATSICLKIYAFIDYSKHHKSLCFV
EILEKQFI*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5661523-5661550(+) 5661821-5661748(-)
Potential amino acid sequence	MEVARSLRQNKFRIKLCEYSYELLLQYLHKTQALVVLGIINERIDFQANGSGSIFKAE(+) MRSLIIPSTTRACVLWRYWRSNSYEYSHSLILNLFCLKDRATSICLKIYAFIDYSKHHKSLCFV EILEKQFI(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os07g0205200_circ_g.2

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