Plant Circular RNA Database

Detail information of Os02g0550300_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os02g0550300_circ_g.3
					
						ID in PlantcircBase
						osa_circ_014968
					
						Alias
						Os_ciR1639
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 20701108-20701672  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os02g0550300						
					
						Parent gene annotation
						
							PDIL5-3 - Zea mays protein disulfide isomerase. (Os02t0550300-00
)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						7/2
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0550300-00:2

General Information
CircRNA Name	Os02g0550300_circ_g.3
ID in PlantcircBase	osa_circ_014968
Alias	Os_ciR1639
Organism	Oryza sativa
Position	chr2: 20701108-20701672 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os02g0550300
Parent gene annotation	PDIL5-3 - Zea mays protein disulfide isomerase. (Os02t0550300-00 )
Parent gene strand	+
Alternative splicing	NA
Support reads	7/2
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0550300-00:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GATATGATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTCTGTTAACCCAAAGTATC
GTGAGCAGAGCATTTTCTATGGCCCTTTTGATGATGttagatgaagctgctccggtgctggctg
gattgagcacacccatcgtagttgctaaggtgaatgccgacaaatacaaaaagctcggatcaaa
atatggag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 478
Transcript exons: 20701108-20701211,20701299-20701672
TTAGATGAAGCTGCTCCGGTGCTGGCTGGATTGAGCACACCCATCGTAGTTGCTAAGGTGAATG
CCGACAAATACAAAAAGCTCGGATCAAAATATGGAGTAGATGGGTTCCCCACTCTGATGCTCTT
TGACCACGGAACCCCAACAGAGTACACTGGTTCAAGGAAGGCCGATTTGCTCGTTGAGAACCTT
AAGAAGCTTGTTGCGCCTGATGTTTCTGTTCTCGAATCGGACTCAGCGATCAAAAGCTTCGTTG
AGGATGCTGGCATGGGTTTTCCATTGTTTCTCGGGTTTGGGGTGGATGAATCCTTGATTGTTGA
GTATGGAGCGAAGTACAAGAACAGGGCCTGGTTTTCTGTAGCAAAGGATTTCTCTGAAGATATG
ATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTCTGTTAACCCAAAGTATCGTGAGC
AGAGCATTTTCTATGGCCCTTTTGATGATG
						
						Genomic sequence
						
							TTAGATGAAGCTGCTCCGGTGCTGGCTGGATTGAGCACACCCATCGTAGTTGCTAAGGTGAATG
CCGACAAATACAAAAAGCTCGGATCAAAATATGGAGTAGAGTAAGGCACCACCTTAAATAGAGA
TGTGTGGTTCACTGAACACTGAAAAGATTGAGATGCCTAATGTTTCTGCATTTCTAAATGCAGT
GGGTTCCCCACTCTGATGCTCTTTGACCACGGAACCCCAACAGAGTACACTGGTTCAAGGAAGG
CCGATTTGCTCGTTGAGAACCTTAAGAAGCTTGTTGCGCCTGATGTTTCTGTTCTCGAATCGGA
CTCAGCGATCAAAAGCTTCGTTGAGGATGCTGGCATGGGTTTTCCATTGTTTCTCGGGTTTGGG
GTGGATGAATCCTTGATTGTTGAGTATGGAGCGAAGTACAAGAACAGGGCCTGGTTTTCTGTAG
CAAAGGATTTCTCTGAAGATATGATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTC
TGTTAACCCAAAGTATCGTGAGCAGAGCATTTTCTATGGCCCTTTTGATGATG

Sequences
Splice junction sequence	GATATGATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTCTGTTAACCCAAAGTATC GTGAGCAGAGCATTTTCTATGGCCCTTTTGATGATGttagatgaagctgctccggtgctggctg gattgagcacacccatcgtagttgctaaggtgaatgccgacaaatacaaaaagctcggatcaaa atatggag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 478 Transcript exons: 20701108-20701211,20701299-20701672 TTAGATGAAGCTGCTCCGGTGCTGGCTGGATTGAGCACACCCATCGTAGTTGCTAAGGTGAATG CCGACAAATACAAAAAGCTCGGATCAAAATATGGAGTAGATGGGTTCCCCACTCTGATGCTCTT TGACCACGGAACCCCAACAGAGTACACTGGTTCAAGGAAGGCCGATTTGCTCGTTGAGAACCTT AAGAAGCTTGTTGCGCCTGATGTTTCTGTTCTCGAATCGGACTCAGCGATCAAAAGCTTCGTTG AGGATGCTGGCATGGGTTTTCCATTGTTTCTCGGGTTTGGGGTGGATGAATCCTTGATTGTTGA GTATGGAGCGAAGTACAAGAACAGGGCCTGGTTTTCTGTAGCAAAGGATTTCTCTGAAGATATG ATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTCTGTTAACCCAAAGTATCGTGAGC AGAGCATTTTCTATGGCCCTTTTGATGATG
Genomic sequence	TTAGATGAAGCTGCTCCGGTGCTGGCTGGATTGAGCACACCCATCGTAGTTGCTAAGGTGAATG CCGACAAATACAAAAAGCTCGGATCAAAATATGGAGTAGAGTAAGGCACCACCTTAAATAGAGA TGTGTGGTTCACTGAACACTGAAAAGATTGAGATGCCTAATGTTTCTGCATTTCTAAATGCAGT GGGTTCCCCACTCTGATGCTCTTTGACCACGGAACCCCAACAGAGTACACTGGTTCAAGGAAGG CCGATTTGCTCGTTGAGAACCTTAAGAAGCTTGTTGCGCCTGATGTTTCTGTTCTCGAATCGGA CTCAGCGATCAAAAGCTTCGTTGAGGATGCTGGCATGGGTTTTCCATTGTTTCTCGGGTTTGGG GTGGATGAATCCTTGATTGTTGAGTATGGAGCGAAGTACAAGAACAGGGCCTGGTTTTCTGTAG CAAAGGATTTCTCTGAAGATATGATGGTCTTTTATGATTTCGACAAGGTCCCTGCATTGGTTTC TGTTAACCCAAAGTATCGTGAGCAGAGCATTTTCTATGGCCCTTTTGATGATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_003999* osi_circ_011859 						
					
						PMCS
						0.392157729

Conservation Information
Conserved circRNAs	osi_circ_003999* osi_circ_011859
PMCS	0.392157729

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20701316-20701112(+)
20701590-20701112(+)
20701465-20701431(-)
						
						Potential amino acid sequence
						
							MLFDHGTPTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGVDESL
IVEYGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKYREQSIFYGPFDDVR*(+)
MISTRSLHWFLLTQSIVSRAFSMALLMMLDEAAPVLAGLSTPIVVAKVNADKYKKLGSKYGVDG
FPTLMLFDHGTPTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGV
DESLIVEYGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKYREQSIFYGPFDDVR*(+
)
MPASSTKLLIAESDSRTETSGATSFLRFSTSKSAFLEPVYSVGVPWSKSIRVGNPSTPYFDPSF
LYLSAFTLATTMGVLNPASTGAASSNIIKRAIENALLTILWVNRNQCRDLVEIIKDHHIFREIL
CYRKPGPVLVLRSILNNQGFIHPKPEKQWKTHASILNEAFDR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	20701316-20701112(+) 20701590-20701112(+) 20701465-20701431(-)
Potential amino acid sequence	MLFDHGTPTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGVDESL IVEYGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKYREQSIFYGPFDDVR(+) MISTRSLHWFLLTQSIVSRAFSMALLMMLDEAAPVLAGLSTPIVVAKVNADKYKKLGSKYGVDG FPTLMLFDHGTPTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGV DESLIVEYGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKYREQSIFYGPFDDVR(+ ) MPASSTKLLIAESDSRTETSGATSFLRFSTSKSAFLEPVYSVGVPWSKSIRVGNPSTPYFDPSF LYLSAFTLATTMGVLNPASTGAASSNIIKRAIENALLTILWVNRNQCRDLVEIIKDHHIFREIL CYRKPGPVLVLRSILNNQGFIHPKPEKQWKTHASILNEAFDR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0550300_circ_g.3

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