Plant Circular RNA Database

Detail information of Csa3G124950_circ_g.5

			
						 General Information
					
						CircRNA Name
						Csa3G124950_circ_g.5
					
						ID in PlantcircBase
						csa_circ_001502
					
						Alias
						Chr3:7562247_7564608
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr3: 7562247-7564608  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa3G124950						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Csa3G124950_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa3G124950.1:4

General Information
CircRNA Name	Csa3G124950_circ_g.5
ID in PlantcircBase	csa_circ_001502
Alias	Chr3:7562247_7564608
Organism	Cucumis sativus
Position	chrChr3: 7562247-7564608 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa3G124950
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Csa3G124950_circ_g.4
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa3G124950.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTTCAAAGCGATGACCAGAATCATA
AAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAATctcactcgttgatcccctgtacttgaac
cacccacaatagagagccattctgcacaatgaattgtagcttcaatatcctgaaaaatggaagt
ggtgacag

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 431
Transcript exons: 7562247-7562323,7562470-7562583,7562766-7562870,7564474-
7564608
CTCACTCGTTGATCCCCTGTACTTGAACCACCCACAATAGAGAGCCATTCTGCACAATGAATTG
TAGCTTCAATATCCTTCCATCCATCCTTCTGGCGCATTGAATGCTCTAACATGATGATCAGAAA
ATTATGTATAAGATCTTCCACATCTCCGACACTTGTAGTCTGAGAATTTTTGCTCTCTCCCAAC
TCATGGTTGGAAATCAAAATCTCAAGAATCCACTCAGGCCATTCTTCCATTTTGGTTAAACTGT
TCCTGTTTTCTGGATGACTGCAAGCCAAAAACAGAAGATCCTGCAGTGCTCGGGATTGGAAAGC
CCTTGATGCATAGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTTCAAAGCGATGA
CCAGAATCATAAAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAAT
						
					

						Genomic sequence
						
							CTCACTCGTTGATCCCCTGTACTTGAACCACCCACAATAGAGAGCCATTCTGCACAATGAATTG
TAGCTTCAATATCCTGAAAAATGGAAGTGGTGACAGAACAAAAATAAATATGACTGATTGAAAC
TAGATATAAGGAGGTAATACGATCAACATGATAGTCACAAAATAAAATAAGATTTAACCAGTTT
TATGCGAAAGAACAGGAAAATAAAATTTCACCTTCCATCCATCCTTCTGGCGCATTGAATGCTC
TAACATGATGATCAGAAAATTATGTATAAGATCTTCCACATCTCCGACACTTGTAGTCTGAGAA
TTTTTGCTCTCTCCCAACTGCAAAGAATGAGCCAGAAATGGAAAATGAAATATGGTAAAAGGAA
TGTTCAAAGCCTTCCCAACGAACCGGTAGTACAAGATTCCCATAAATACAATTTATAATTGGAT
ATGTGTACTACTCGGCCATTCATCTACCACAGTCAAGTTCATAAATTCTAAACTTACACCAGAT
AACATACCTCATGGTTGGAAATCAAAATCTCAAGAATCCACTCAGGCCATTCTTCCATTTTGGT
TAAACTGTTCCTGTTTTCTGGATGACTGCAAGCCAAAAACAGAAGATCCTGGAATCATATACCA
AAAAGTTTAGAAACTAAAACAGGTAATCAATAAAAACAGTAACAATAATCAATTAAATATGGAG
AGATGAATGACTAAAAACAACAGCAACAGTGAATGAAAATCAGAAATCTAACAGTGTTTAACTG
ATTCCACATAAGCACATCGATGGATCTCATTATTGATGTGTACGGTACGAAACGTGTGTGGGTG
GATGGGTCAGGGAATAACTTGCTTTATAGATAATTTGATGCATTGAGTGAAGGCCGTGGGCTAA
TCAGTGGCTGATGGGGGGAGACGTATCCAAGATGTATTATGAGAGTAAATTGAGTAAATGGAAG
TTTTTTTGGATGTATTAGAAGGTCTAGAATAAGGAGATTTTAAGGAGTAGTAGGAAACTCTTGA
AATTTTCATCAAGCTGTACACACATGAAACTCTTTTAAATCACATGCATTCAGTGGGTCACTGG
GGCTAGTCACTAGTCAATGACTGATGGGTGGGGTGGGGGGGAGAGATGTATCAGAGTCAATAGG
AAGATCTTGAGTAGATGGGAATTTTCTTGGCTGTAATAGAAGGTCTAGAATACGGAGTGATAGG
AAACTCTTGAAATTCTCATCAAGTTGAACATGCCTGAAACTATTTAAAGTCGTATGCATTCTAT
CACACATCTCTCAAGAAGCAACAAATAGTTTTTTTGTCTATTTAGCTTAGTTTGGCTCCTTTTT
GGGTTTCCCTTGTCACCTTGTGTAACCCATCCTTTTTGTTGGCCTGGGTTGCTGCATCCATTTT
CTTTGTTCTCGTGTATTCTTTGATTTTTCCCTCCATAAATTTTGTGCTAATCCCTTTTTTTAAA
CATAAAAAATAATTTAAAGAAGAAAACAACAACTACCACAGTCCACAACTACTGAGTAAGGTGC
ACAATGACTGACGTGGTCCCCTCTTAGTTGTAAACTTAAGTTCCCATCTTCTGAAAATGGTAAG
TTACTTGGTTATCAAGAGGTTGGGTTGGTTGTTAGTTTTGTTAATCGACTGACTGCTGTAACTA
AATAATCGGCCAATTGTTTTTCAACCTAACTAATTGTGAACAAACAGCTGGTATAGTTGTATCT
ATTTCATTCAAAATAACACAATTTATGATACTTTTCATGACATTAACGACAATATAATCATTGT
AGACAACCAAGAAGGAAAAAAAAAAAGCTTTTAACATGCATGGATAGATTAGCCTCTTTGAGCA
TTAGTTCCTTAGCATTTTAACAATGAAAAGTTATGTTTCCTTTTCAAGAAAAAATATGCATGGA
TAGATTCCAGTGCAGTACCAAACCTATGCAAGGAAATTTCCATGCAACTCATAAATTTCCTTAC
ATGATACCTTTTCCTTTTTGTGGATGAGAGACTAAGTAGTATGATGATTGATGAATAACGGCAG
AGAACAAACTGATGGAAAAGGCTATGGTCCACCGAAGGACCAAATCCAATTACAAAATGTGTTT
CCAGTTCTAGAAGGATGAAAAATAGAAAGATAATAAACCATATTCATTACAGTTCCTAAAAAAC
AGAGATTGCAGCATGTAACAAGAAACAATGCCTAGTTATCATTGGGCATACCTGCAGTGCTCGG
GATTGGAAAGCCCTTGATGCATAGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTT
CAAAGCGATGACCAGAATCATAAAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAAT
						
					

			
		

Sequences
Splice junction sequence	AGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTTCAAAGCGATGACCAGAATCATA AAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAATctcactcgttgatcccctgtacttgaac cacccacaatagagagccattctgcacaatgaattgtagcttcaatatcctgaaaaatggaagt ggtgacag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 431 Transcript exons: 7562247-7562323,7562470-7562583,7562766-7562870,7564474- 7564608 CTCACTCGTTGATCCCCTGTACTTGAACCACCCACAATAGAGAGCCATTCTGCACAATGAATTG TAGCTTCAATATCCTTCCATCCATCCTTCTGGCGCATTGAATGCTCTAACATGATGATCAGAAA ATTATGTATAAGATCTTCCACATCTCCGACACTTGTAGTCTGAGAATTTTTGCTCTCTCCCAAC TCATGGTTGGAAATCAAAATCTCAAGAATCCACTCAGGCCATTCTTCCATTTTGGTTAAACTGT TCCTGTTTTCTGGATGACTGCAAGCCAAAAACAGAAGATCCTGCAGTGCTCGGGATTGGAAAGC CCTTGATGCATAGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTTCAAAGCGATGA CCAGAATCATAAAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAAT
Genomic sequence	CTCACTCGTTGATCCCCTGTACTTGAACCACCCACAATAGAGAGCCATTCTGCACAATGAATTG TAGCTTCAATATCCTGAAAAATGGAAGTGGTGACAGAACAAAAATAAATATGACTGATTGAAAC TAGATATAAGGAGGTAATACGATCAACATGATAGTCACAAAATAAAATAAGATTTAACCAGTTT TATGCGAAAGAACAGGAAAATAAAATTTCACCTTCCATCCATCCTTCTGGCGCATTGAATGCTC TAACATGATGATCAGAAAATTATGTATAAGATCTTCCACATCTCCGACACTTGTAGTCTGAGAA TTTTTGCTCTCTCCCAACTGCAAAGAATGAGCCAGAAATGGAAAATGAAATATGGTAAAAGGAA TGTTCAAAGCCTTCCCAACGAACCGGTAGTACAAGATTCCCATAAATACAATTTATAATTGGAT ATGTGTACTACTCGGCCATTCATCTACCACAGTCAAGTTCATAAATTCTAAACTTACACCAGAT AACATACCTCATGGTTGGAAATCAAAATCTCAAGAATCCACTCAGGCCATTCTTCCATTTTGGT TAAACTGTTCCTGTTTTCTGGATGACTGCAAGCCAAAAACAGAAGATCCTGGAATCATATACCA AAAAGTTTAGAAACTAAAACAGGTAATCAATAAAAACAGTAACAATAATCAATTAAATATGGAG AGATGAATGACTAAAAACAACAGCAACAGTGAATGAAAATCAGAAATCTAACAGTGTTTAACTG ATTCCACATAAGCACATCGATGGATCTCATTATTGATGTGTACGGTACGAAACGTGTGTGGGTG GATGGGTCAGGGAATAACTTGCTTTATAGATAATTTGATGCATTGAGTGAAGGCCGTGGGCTAA TCAGTGGCTGATGGGGGGAGACGTATCCAAGATGTATTATGAGAGTAAATTGAGTAAATGGAAG TTTTTTTGGATGTATTAGAAGGTCTAGAATAAGGAGATTTTAAGGAGTAGTAGGAAACTCTTGA AATTTTCATCAAGCTGTACACACATGAAACTCTTTTAAATCACATGCATTCAGTGGGTCACTGG GGCTAGTCACTAGTCAATGACTGATGGGTGGGGTGGGGGGGAGAGATGTATCAGAGTCAATAGG AAGATCTTGAGTAGATGGGAATTTTCTTGGCTGTAATAGAAGGTCTAGAATACGGAGTGATAGG AAACTCTTGAAATTCTCATCAAGTTGAACATGCCTGAAACTATTTAAAGTCGTATGCATTCTAT CACACATCTCTCAAGAAGCAACAAATAGTTTTTTTGTCTATTTAGCTTAGTTTGGCTCCTTTTT GGGTTTCCCTTGTCACCTTGTGTAACCCATCCTTTTTGTTGGCCTGGGTTGCTGCATCCATTTT CTTTGTTCTCGTGTATTCTTTGATTTTTCCCTCCATAAATTTTGTGCTAATCCCTTTTTTTAAA CATAAAAAATAATTTAAAGAAGAAAACAACAACTACCACAGTCCACAACTACTGAGTAAGGTGC ACAATGACTGACGTGGTCCCCTCTTAGTTGTAAACTTAAGTTCCCATCTTCTGAAAATGGTAAG TTACTTGGTTATCAAGAGGTTGGGTTGGTTGTTAGTTTTGTTAATCGACTGACTGCTGTAACTA AATAATCGGCCAATTGTTTTTCAACCTAACTAATTGTGAACAAACAGCTGGTATAGTTGTATCT ATTTCATTCAAAATAACACAATTTATGATACTTTTCATGACATTAACGACAATATAATCATTGT AGACAACCAAGAAGGAAAAAAAAAAAGCTTTTAACATGCATGGATAGATTAGCCTCTTTGAGCA TTAGTTCCTTAGCATTTTAACAATGAAAAGTTATGTTTCCTTTTCAAGAAAAAATATGCATGGA TAGATTCCAGTGCAGTACCAAACCTATGCAAGGAAATTTCCATGCAACTCATAAATTTCCTTAC ATGATACCTTTTCCTTTTTGTGGATGAGAGACTAAGTAGTATGATGATTGATGAATAACGGCAG AGAACAAACTGATGGAAAAGGCTATGGTCCACCGAAGGACCAAATCCAATTACAAAATGTGTTT CCAGTTCTAGAAGGATGAAAAATAGAAAGATAATAAACCATATTCATTACAGTTCCTAAAAAAC AGAGATTGCAGCATGTAACAAGAAACAATGCCTAGTTATCATTGGGCATACCTGCAGTGCTCGG GATTGGAAAGCCCTTGATGCATAGGGAAGTGAGCGTAAAAGAACCAGCAGTAGTTGTAGGTGTT CAAAGCGATGACCAGAATCATAAAAGTTTAGCCCATCTTCAGTCGAAGAAGCATTAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.49333707

Conservation Information
Conserved circRNAs	NA
PMCS	0.49333707

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7562818-7564580(-)
7562296-7564580(-)
						
						Potential amino acid sequence
						
							MEEWPEWILEILISNHELGESKNSQTTSVGDVEDLIHNFLIIMLEHSMRQKDGWKDIEATIHCA
EWLSIVGGSSTGDQRVRLMLLRLKMG*
MALYCGWFKYRGSTSEINASSTEDGLNFYDSGHRFEHLQLLLVLLRSLPYASRAFQSRALQDLL
FLACSHPENRNSLTKMEEWPEWILEILISNHELGESKNSQTTSVGDVEDLIHNFLIIMLEHSMR
QKDGWKDIEATIHCAEWLSIVGGSSTGDQRVRLMLLRLKMG*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	7562818-7564580(-) 7562296-7564580(-)
Potential amino acid sequence	MEEWPEWILEILISNHELGESKNSQTTSVGDVEDLIHNFLIIMLEHSMRQKDGWKDIEATIHCA EWLSIVGGSSTGDQRVRLMLLRLKMG* MALYCGWFKYRGSTSEINASSTEDGLNFYDSGHRFEHLQLLLVLLRSLPYASRAFQSRALQDLL FLACSHPENRNSLTKMEEWPEWILEILISNHELGESKNSQTTSVGDVEDLIHNFLIIMLEHSMR QKDGWKDIEATIHCAEWLSIVGGSSTGDQRVRLMLLRLKMG*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa3G124950_circ_g.5

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