Plant Circular RNA Database

Detail information of Os01g0888700_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os01g0888700_circ_g.3
					
						ID in PlantcircBase
						osa_circ_005160
					
						Alias
						Os_ciR2755
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 38633250-38633703  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os01g0888750						
					
						Parent gene annotation
						
							Hypothetical protein. (Os01t0888750-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0888700_circ_g.1 Os01g0888700_circ_g.2 Os01g0888700_circ_g.4 						
					
						Support reads
						4/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0888700-01:3
Os01t0888700-01:3
Os01t0888750-00:3

General Information
CircRNA Name	Os01g0888700_circ_g.3
ID in PlantcircBase	osa_circ_005160
Alias	Os_ciR2755
Organism	Oryza sativa
Position	chr1: 38633250-38633703 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os01g0888750
Parent gene annotation	Hypothetical protein. (Os01t0888750-00)
Parent gene strand	+
Alternative splicing	Os01g0888700_circ_g.1 Os01g0888700_circ_g.2 Os01g0888700_circ_g.4
Support reads	4/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0888700-01:3 Os01t0888700-01:3 Os01t0888750-00:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTAACTACTAGAATATGTCACATACATCTGGGCATTCCGATGTTTAACAGATACCATCTGCGG
GAAGTCAATCATTGTAACTTTCTCATCATCATCAATcttgtccatctcaacttgctcttttcgg
gtgaaaccactggcagctagctccttgtccaatgaaccagcagccttttgtatagacagaaagg
atggcctg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 288
Transcript exons: 38633250-38633415,38633509-38633556,38633630-38633703
CTTGTCCATCTCAACTTGCTCTTTTCGGGTGAAACCACTGGCAGCTAGCTCCTTGTCCAATGAA
CCAGCAGCCTTTTGTATAGACAGAAAGGATGGCCTGCTATTCTCATCATCATCAATGTCTGACC
CATCCTGTTCTTCACATTTCTCTGAGGAAAGATGAAACCTCTTCCTAAAGAATTTGTAGATGCA
TTCAATATCTCGATCAAAAAACATCTGGGCATTCCGATGTTTAACAGATACCATCTGCGGGAAG
TCAATCATTGTAACTTTCTCATCATCATCAAT
						
						Genomic sequence
						
							CTTGTCCATCTCAACTTGCTCTTTTCGGGTGAAACCACTGGCAGCTAGCTCCTTGTCCAATGAA
CCAGCAGCCTTTTGTATAGACAGAAAGGATGGCCTGCTATTCTCATCATCATCAATGTCTGACC
CATCCTGTTCTTCACATTTCTCTGAGGAAAGATGAAACCTACAATAAATAAACAAAAGAAAGCT
TATTGACAAATGTGTTGAAGTGCACCAAAATGAATTGTTGAGGTTCCAGCATTAAATAGACAAT
AACCTCTTCCTAAAGAATTTGTAGATGCATTCAATATCTCGATCAAAAAACCTGCAAAAGTAAA
ACTCATGAATAATGAATACAAAGGTAGTCTTTCTCTTAACTACTAGAATATGTCACATACATCT
GGGCATTCCGATGTTTAACAGATACCATCTGCGGGAAGTCAATCATTGTAACTTTCTCATCATC
ATCAAT

Sequences
Splice junction sequence	CTTAACTACTAGAATATGTCACATACATCTGGGCATTCCGATGTTTAACAGATACCATCTGCGG GAAGTCAATCATTGTAACTTTCTCATCATCATCAATcttgtccatctcaacttgctcttttcgg gtgaaaccactggcagctagctccttgtccaatgaaccagcagccttttgtatagacagaaagg atggcctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 288 Transcript exons: 38633250-38633415,38633509-38633556,38633630-38633703 CTTGTCCATCTCAACTTGCTCTTTTCGGGTGAAACCACTGGCAGCTAGCTCCTTGTCCAATGAA CCAGCAGCCTTTTGTATAGACAGAAAGGATGGCCTGCTATTCTCATCATCATCAATGTCTGACC CATCCTGTTCTTCACATTTCTCTGAGGAAAGATGAAACCTCTTCCTAAAGAATTTGTAGATGCA TTCAATATCTCGATCAAAAAACATCTGGGCATTCCGATGTTTAACAGATACCATCTGCGGGAAG TCAATCATTGTAACTTTCTCATCATCATCAAT
Genomic sequence	CTTGTCCATCTCAACTTGCTCTTTTCGGGTGAAACCACTGGCAGCTAGCTCCTTGTCCAATGAA CCAGCAGCCTTTTGTATAGACAGAAAGGATGGCCTGCTATTCTCATCATCATCAATGTCTGACC CATCCTGTTCTTCACATTTCTCTGAGGAAAGATGAAACCTACAATAAATAAACAAAAGAAAGCT TATTGACAAATGTGTTGAAGTGCACCAAAATGAATTGTTGAGGTTCCAGCATTAAATAGACAAT AACCTCTTCCTAAAGAATTTGTAGATGCATTCAATATCTCGATCAAAAAACCTGCAAAAGTAAA ACTCATGAATAATGAATACAAAGGTAGTCTTTCTCTTAACTACTAGAATATGTCACATACATCT GGGCATTCCGATGTTTAACAGATACCATCTGCGGGAAGTCAATCATTGTAACTTTCTCATCATC ATCAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_011031 						
					
						PMCS
						0.283095466

Conservation Information
Conserved circRNAs	osi_circ_011031
PMCS	0.283095466

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							38633410-38633294(+)
38633531-38633409(+)
38633681-38633252(-)
						
						Potential amino acid sequence
						
							MKPLPKEFVDAFNISIKKHLGIPMFNRYHLREVNHCNFLIIINLVHLNLLFSGETTGS*(+)
MHSISRSKNIWAFRCLTDTICGKSIIVTFSSSSILSISTCSFRVKPLAASSLSNEPAAFCIDRK
DGLLFSSSSMSDPSCSSHFSEER*(+)
MIDFPQMVSVKHRNAQMFFDRDIECIYKFFRKRFHLSSEKCEEQDGSDIDDDENSRPSFLSIQK
AAGSLDKELAASGFTRKEQVEMDKIDDDEKVTMIDFPQMVSVKHRNAQMFFDRDIECIYKFFRK
RFHLSSEKCEEQDGSDIDDDENSRPSFLSIQKAAGSLDKELAASGFTRKEQVEMDKIDDDEKVT
MIDFPQMVSVKHRNAQMFFDRDIECIYKFFRKRFHLSSEKCEEQDGSDIDDDENSRPSFLSIQK
AAGSLDKELAASGFTRKEQVEMDKIDDDEKVTMIDFPQMVSVKHRNAQMFFDRDIECIYKFFRK
RFHLSSEKCEEQDGSDIDDDENSRPSFLSIQKAAGSLDKELAASGFTRKEQVEMDK(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	38633410-38633294(+) 38633531-38633409(+) 38633681-38633252(-)
Potential amino acid sequence	MKPLPKEFVDAFNISIKKHLGIPMFNRYHLREVNHCNFLIIINLVHLNLLFSGETTGS(+) MHSISRSKNIWAFRCLTDTICGKSIIVTFSSSSILSISTCSFRVKPLAASSLSNEPAAFCIDRK DGLLFSSSSMSDPSCSSHFSEER(+) MIDFPQMVSVKHRNAQMFFDRDIECIYKFFRKRFHLSSEKCEEQDGSDIDDDENSRPSFLSIQK AAGSLDKELAASGFTRKEQVEMDKIDDDEKVTMIDFPQMVSVKHRNAQMFFDRDIECIYKFFRK RFHLSSEKCEEQDGSDIDDDENSRPSFLSIQKAAGSLDKELAASGFTRKEQVEMDKIDDDEKVT MIDFPQMVSVKHRNAQMFFDRDIECIYKFFRKRFHLSSEKCEEQDGSDIDDDENSRPSFLSIQK AAGSLDKELAASGFTRKEQVEMDKIDDDEKVTMIDFPQMVSVKHRNAQMFFDRDIECIYKFFRK RFHLSSEKCEEQDGSDIDDDENSRPSFLSIQKAAGSLDKELAASGFTRKEQVEMDK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0888700_circ_g.3

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