Plant Circular RNA Database

Detail information of Os02g0216300_circ_g.57

			
						 General Information
					
						CircRNA Name
						Os02g0216300_circ_g.57
					
						ID in PlantcircBase
						osa_circ_013875
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 6513669-6513827  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						PcircRNA_finder
					
						Parent gene
						
							Os02g0216300						
					
						Parent gene annotation
						
							Similar to cDNA clone:J023088C01, full insert sequence. (Os02t02
16300-01);Similar to cDNA clone:J023088C01, full insert sequence
. (Os02t0216300-04);Similar to cDNA clone:J023088C01, full inser
t sequence. (Os02t0216300-05);Similar to cDNA clone:J023088C01, 
full insert sequence. (Os02t0216300-07);Similar to cDNA clone:00
1-032-A03, full insert sequence. (Os02t0216300-09)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						pistil
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GG-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0216300-09:1
Os02t0216300-07:1
Os02t0216300-04:1
Os02t0216300-05:1

General Information
CircRNA Name	Os02g0216300_circ_g.57
ID in PlantcircBase	osa_circ_013875
Alias	NA
Organism	Oryza sativa
Position	chr2: 6513669-6513827 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	PcircRNA_finder
Parent gene	Os02g0216300
Parent gene annotation	Similar to cDNA clone:J023088C01, full insert sequence. (Os02t02 16300-01);Similar to cDNA clone:J023088C01, full insert sequence . (Os02t0216300-04);Similar to cDNA clone:J023088C01, full inser t sequence. (Os02t0216300-05);Similar to cDNA clone:J023088C01, full insert sequence. (Os02t0216300-07);Similar to cDNA clone:00 1-032-A03, full insert sequence. (Os02t0216300-09)
Parent gene strand	-
Alternative splicing	NA
Support reads	1
Tissues	pistil
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GG-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0216300-09:1 Os02t0216300-07:1 Os02t0216300-04:1 Os02t0216300-05:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCACAAAAGAAAAACCGTC
ACTAATTGTTGTCATcggaagaagataaccgccgccgccgaagcagctagcaggcacaaggcgc
aagatcaagctcgctcgcgcgacgccggtgc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 159
Transcript exons: 6513669-6513827
CGGAAGAAGATAACCGCCGCCGCCGAAGCAGCTAGCAGGCACAAGGCGCAAGATCAAGCTCGCT
CGCGCGACGCCGGTGCCGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCA
CAAAAGAAAAACCGTCACTAATTGTTGTCAT
						
						Genomic sequence
						
							CGGAAGAAGATAACCGCCGCCGCCGAAGCAGCTAGCAGGCACAAGGCGCAAGATCAAGCTCGCT
CGCGCGACGCCGGTGCCGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCA
CAAAAGAAAAACCGTCACTAATTGTTGTCAT

Sequences
Splice junction sequence	CGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCACAAAAGAAAAACCGTC ACTAATTGTTGTCATcggaagaagataaccgccgccgccgaagcagctagcaggcacaaggcgc aagatcaagctcgctcgcgcgacgccggtgc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 159 Transcript exons: 6513669-6513827 CGGAAGAAGATAACCGCCGCCGCCGAAGCAGCTAGCAGGCACAAGGCGCAAGATCAAGCTCGCT CGCGCGACGCCGGTGCCGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCA CAAAAGAAAAACCGTCACTAATTGTTGTCAT
Genomic sequence	CGGAAGAAGATAACCGCCGCCGCCGAAGCAGCTAGCAGGCACAAGGCGCAAGATCAAGCTCGCT CGCGCGACGCCGGTGCCGTCTGGTAATCGACGAAGCTGGAAGTAAACCTACACTATATGTGTCA CAAAAGAAAAACCGTCACTAATTGTTGTCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.351025943

Conservation Information
Conserved circRNAs	NA
PMCS	0.351025943

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6513790-6513755(+)
						
						Potential amino acid sequence
						
							MCHKRKTVTNCCHRKKITAAAEAASRHKAQDQARSRDAGAVW*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	6513790-6513755(+)
Potential amino acid sequence	MCHKRKTVTNCCHRKKITAAAEAASRHKAQDQARSRDAGAVW*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0216300_circ_g.57

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