Plant Circular RNA Database

Detail information of Solyc09g018060.2_circ_g.2

			
						 General Information
					
						CircRNA Name
						Solyc09g018060.2_circ_g.2
					
						ID in PlantcircBase
						sly_circ_002826
					
						Alias
						Slcirc131
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr9: 12708685-12714300  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Solyc09g018060.2						
					
						Parent gene annotation
						
							protein_coding
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Solyc09g018060.2_circ_g.1 Solyc09g018060.2_circ_g.3 Solyc09g018060.2_circ_g.4 						
					
						Support reads
						8/32
					
						Tissues
						fruit/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc09g018060.2.1:4

General Information
CircRNA Name	Solyc09g018060.2_circ_g.2
ID in PlantcircBase	sly_circ_002826
Alias	Slcirc131
Organism	Solanum lycopersicum
Position	chr9: 12708685-12714300 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	CIRI
Parent gene	Solyc09g018060.2
Parent gene annotation	protein_coding
Parent gene strand	-
Alternative splicing	Solyc09g018060.2_circ_g.1 Solyc09g018060.2_circ_g.3 Solyc09g018060.2_circ_g.4
Support reads	8/32
Tissues	fruit/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc09g018060.2.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: TTGGAAGGAAAAGAAAAATGATAC
 R: TGACAGCAGAAACTGGGACATA
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: TTGGAAGGAAAAGAAAAATGATAC R: TGACAGCAGAAACTGGGACATA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CATAACAACTTCCCGAACAAAACGGCCTTCAAGTGCAGATCTTTCTTCTGAGGTGTTACCACCA
TTAAGAACTTTTATTGCAACAATTTGATCACCATACcttaaaagcagcagcataagcagcttgc
aaattcgacattccctcaaaaggcatgcggtttgtcagtaattcccacaagacaataccaaaac
tataaaca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 613
Transcript exons: 12708685-12708843,12713068-12713192,12713710-12713905,12
714168-12714300
CTTAAAAGCAGCAGCATAAGCAGCTTGCAAATTCGACATTCCCTCAAAAGGCATGCGGTTTGTC
AGTAATTCCCACAAGACAATACCAAAACTATAAACATCGACCTTGTTATTATAATGCTTCTTCT
CCCCCAGACGCAATGTAACAGTGCTGTACAACTCAGGTGCCATCCACCGGTATGTCCCAGTTTC
TGCTGTCATCATCTCTGTAAGGGTCTCTTCCCTTGCTAACCCAAAATCAGCAAGCTTCACAGAC
TTCTGATTGGCCGTGAGCAGCAAATTGTCAGGTTTTAGATCTCTATGTATAATGCCATTGGCAT
GTAAACATTCCATGGCCCGAGCAATGTCAAGAGCATAATTTATGGCGACGTGTAGGTCAAGCAC
GTTTGGTCGAATGCTGACTAAGTACTTCCGAAGAGACATTCCAGGCAGTAGCTCTGAAACTATA
ACCATCAAAGGATCCTTGCAAGCTCCAATAAATTTTACAAGATTCTCATGTTTTACTCTTGACA
TCATAACAACTTCCCGAACAAAACGGCCTTCAAGTGCAGATCTTTCTTCTGAGGTGTTACCACC
ATTAAGAACTTTTATTGCAACAATTTGATCACCATAC
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CATAACAACTTCCCGAACAAAACGGCCTTCAAGTGCAGATCTTTCTTCTGAGGTGTTACCACCA TTAAGAACTTTTATTGCAACAATTTGATCACCATACcttaaaagcagcagcataagcagcttgc aaattcgacattccctcaaaaggcatgcggtttgtcagtaattcccacaagacaataccaaaac tataaaca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 613 Transcript exons: 12708685-12708843,12713068-12713192,12713710-12713905,12 714168-12714300 CTTAAAAGCAGCAGCATAAGCAGCTTGCAAATTCGACATTCCCTCAAAAGGCATGCGGTTTGTC AGTAATTCCCACAAGACAATACCAAAACTATAAACATCGACCTTGTTATTATAATGCTTCTTCT CCCCCAGACGCAATGTAACAGTGCTGTACAACTCAGGTGCCATCCACCGGTATGTCCCAGTTTC TGCTGTCATCATCTCTGTAAGGGTCTCTTCCCTTGCTAACCCAAAATCAGCAAGCTTCACAGAC TTCTGATTGGCCGTGAGCAGCAAATTGTCAGGTTTTAGATCTCTATGTATAATGCCATTGGCAT GTAAACATTCCATGGCCCGAGCAATGTCAAGAGCATAATTTATGGCGACGTGTAGGTCAAGCAC GTTTGGTCGAATGCTGACTAAGTACTTCCGAAGAGACATTCCAGGCAGTAGCTCTGAAACTATA ACCATCAAAGGATCCTTGCAAGCTCCAATAAATTTTACAAGATTCTCATGTTTTACTCTTGACA TCATAACAACTTCCCGAACAAAACGGCCTTCAAGTGCAGATCTTTCTTCTGAGGTGTTACCACC ATTAAGAACTTTTATTGCAACAATTTGATCACCATAC
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.820070599

Conservation Information
Conserved circRNAs	NA
PMCS	0.820070599

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12714205-12714294(-)
12708703-12714294(-)
						
						Potential amino acid sequence
						
							MMSRVKHENLVKFIGACKDPLMVIVSELLPGMSLRKYLVSIRPNVLDLHVAINYALDIARAMEC
LHANGIIHRDLKPDNLLLTANQKSVKLADFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRL
GEKKHYNNKVDVYSFGIVLWELLTNRMPFEGMSNLQAAYAAAFKVW*(-)
MLLLLRYGDQIVAIKVLNGGNTSEERSALEGRFVREVVMMSRVKHENLVKFIGACKDPLMVIVS
ELLPGMSLRKYLVSIRPNVLDLHVAINYALDIARAMECLHANGIIHRDLKPDNLLLTANQKSVK
LADFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRLGEKKHYNNKVDVYSFGIVLWELLTNR
MPFEGMSNLQAAYAAAFKVW*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12714205-12714294(-) 12708703-12714294(-)
Potential amino acid sequence	MMSRVKHENLVKFIGACKDPLMVIVSELLPGMSLRKYLVSIRPNVLDLHVAINYALDIARAMEC LHANGIIHRDLKPDNLLLTANQKSVKLADFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRL GEKKHYNNKVDVYSFGIVLWELLTNRMPFEGMSNLQAAYAAAFKVW(-) MLLLLRYGDQIVAIKVLNGGNTSEERSALEGRFVREVVMMSRVKHENLVKFIGACKDPLMVIVS ELLPGMSLRKYLVSIRPNVLDLHVAINYALDIARAMECLHANGIIHRDLKPDNLLLTANQKSVK LADFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRLGEKKHYNNKVDVYSFGIVLWELLTNR MPFEGMSNLQAAYAAAFKVW(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zuo et al., 2016; Wang et al., 2018b

circRNA

Detail information of Solyc09g018060.2_circ_g.2

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