Plant Circular RNA Database

Detail information of Os01g0775400_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os01g0775400_circ_g.1
					
						ID in PlantcircBase
						osa_circ_004160
					
						Alias
						Os01circ24769
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 32789761-32790782  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, SMALT, Segemehl
					
						Parent gene
						
							Os01g0775400						
					
						Parent gene annotation
						
							Similar to Cytokinin dehydrogenase 5 precursor (EC 1.5.99.12) (C
ytokinin oxidase 5) (CKO5) (AtCKX5) (AtCKX6). (Os01t0775400-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						4/9
					
						Tissues
						panicle/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0775400-01:2

General Information
CircRNA Name	Os01g0775400_circ_g.1
ID in PlantcircBase	osa_circ_004160
Alias	Os01circ24769
Organism	Oryza sativa
Position	chr1: 32789761-32790782 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, SMALT, Segemehl
Parent gene	Os01g0775400
Parent gene annotation	Similar to Cytokinin dehydrogenase 5 precursor (EC 1.5.99.12) (C ytokinin oxidase 5) (CKO5) (AtCKX5) (AtCKX6). (Os01t0775400-01)
Parent gene strand	-
Alternative splicing	NA
Support reads	4/9
Tissues	panicle/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0775400-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CGTAGTCGAACCGCCGGCCGCCGTGCTGCAGGGAGATGAGCCGCTCCTGGTCCGCGGTGAACTC
GGTGAAGTTGGAGTAGAGCGCCCGGATCCAGCGAACttgtgcctgttcatggggtagatgagga
tggggccaccggcggtgcggctgccgaggacgccgcggaagacgccgcggtcgaagtcggcgat
gcgggacg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 536
Transcript exons: 32789761-32790026,32790513-32790782
TTGTGCCTGTTCATGGGGTAGATGAGGATGGGGCCACCGGCGGTGCGGCTGCCGAGGACGCCGC
GGAAGACGCCGCGGTCGAAGTCGGCGATGCGGGACGCCGGCACGAAGAGGTTGAGCCACGGGTG
CGGGACCTCCCACATGCCCTTGCCGCGGAGCTTCAGCTCCGCCTTGTGCACGCGGTCTAGGAAG
TCCACGTACGGCAGGTCCGTCGTGAACACCGTGCCCGGGATGAAATTCAGCTCGCCCAGCAGCG
CCTCCACGTCCTGGTCGACGGTTACGGCGGTGGAGTCGTCGTAATTCTTGGTGACCTCCAGGCA
GTAGAGAACCCCGGAGTGGTGCTTGAGCGAGCTGAGCTTGACCGGGTTCTGCGGCGAGAAGAAG
GACGACCTCCAGTTGTTGATTAGGCCCTCGGCGGCGACGACGAAGCCCTCCACGTAGTCGAACC
GCCGGCCGCCGTGCTGCAGGGAGATGAGCCGCTCCTGGTCCGCGGTGAACTCGGTGAAGTTGGA
GTAGAGCGCCCGGATCCAGCGAAC
						
					

						Genomic sequence
						
							TTGTGCCTGTTCATGGGGTAGATGAGGATGGGGCCACCGGCGGTGCGGCTGCCGAGGACGCCGC
GGAAGACGCCGCGGTCGAAGTCGGCGATGCGGGACGCCGGCACGAAGAGGTTGAGCCACGGGTG
CGGGACCTCCCACATGCCCTTGCCGCGGAGCTTCAGCTCCGCCTTGTGCACGCGGTCTAGGAAG
TCCACGTACGGCAGGTCCGTCGTGAACACCGTGCCCGGGATGAAATTCAGCTCGCCCAGCAGCG
CCTCCACGTCCTGGAAAAAAAAATCGAAACTAATTATTTAAACTCCTAGTTTAGAATGATCTAC
TTCGTCTTTCTTTTTCATCACTTGCGTATAGTACTATACTAGTGGTAATAGCTAGTACTGCGTA
ATTAATTGATTAATTAAGTGGTCGATAAAATAGTGCATGTTTTGTCAGTGGGACTAAGATCTAG
ACAGCTACTAGTACGTTTGCAAGATCCACCGATCCACTGTGCTCAGCATGTGGGTGCAATCATA
CAATCATCAAGGGCTTGGATTACACTAGAGAGCTTGACTAAACCGCCCATAACTAACTAAAAAC
ATGGCCCAATGATTGACTAGGTGCCCTGCCATTAGAGTACTAAACTACTCCAGTAGGAATAATT
AAGAAATTTCGTCATAAACAAATGGGCGGTCAGGGGGGGAGGCAGAGAATTATTGGTGAATCAG
GAAAGACGGCGCCGGCCGCGCGATCGCGCCGGCACGACGGACACGCACCTGGTCGACGGTTACG
GCGGTGGAGTCGTCGTAATTCTTGGTGACCTCCAGGCAGTAGAGAACCCCGGAGTGGTGCTTGA
GCGAGCTGAGCTTGACCGGGTTCTGCGGCGAGAAGAAGGACGACCTCCAGTTGTTGATTAGGCC
CTCGGCGGCGACGACGAAGCCCTCCACGTAGTCGAACCGCCGGCCGCCGTGCTGCAGGGAGATG
AGCCGCTCCTGGTCCGCGGTGAACTCGGTGAAGTTGGAGTAGAGCGCCCGGATCCAGCGAAC
						
					

			
		

Sequences
Splice junction sequence	CGTAGTCGAACCGCCGGCCGCCGTGCTGCAGGGAGATGAGCCGCTCCTGGTCCGCGGTGAACTC GGTGAAGTTGGAGTAGAGCGCCCGGATCCAGCGAACttgtgcctgttcatggggtagatgagga tggggccaccggcggtgcggctgccgaggacgccgcggaagacgccgcggtcgaagtcggcgat gcgggacg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 536 Transcript exons: 32789761-32790026,32790513-32790782 TTGTGCCTGTTCATGGGGTAGATGAGGATGGGGCCACCGGCGGTGCGGCTGCCGAGGACGCCGC GGAAGACGCCGCGGTCGAAGTCGGCGATGCGGGACGCCGGCACGAAGAGGTTGAGCCACGGGTG CGGGACCTCCCACATGCCCTTGCCGCGGAGCTTCAGCTCCGCCTTGTGCACGCGGTCTAGGAAG TCCACGTACGGCAGGTCCGTCGTGAACACCGTGCCCGGGATGAAATTCAGCTCGCCCAGCAGCG CCTCCACGTCCTGGTCGACGGTTACGGCGGTGGAGTCGTCGTAATTCTTGGTGACCTCCAGGCA GTAGAGAACCCCGGAGTGGTGCTTGAGCGAGCTGAGCTTGACCGGGTTCTGCGGCGAGAAGAAG GACGACCTCCAGTTGTTGATTAGGCCCTCGGCGGCGACGACGAAGCCCTCCACGTAGTCGAACC GCCGGCCGCCGTGCTGCAGGGAGATGAGCCGCTCCTGGTCCGCGGTGAACTCGGTGAAGTTGGA GTAGAGCGCCCGGATCCAGCGAAC
Genomic sequence	TTGTGCCTGTTCATGGGGTAGATGAGGATGGGGCCACCGGCGGTGCGGCTGCCGAGGACGCCGC GGAAGACGCCGCGGTCGAAGTCGGCGATGCGGGACGCCGGCACGAAGAGGTTGAGCCACGGGTG CGGGACCTCCCACATGCCCTTGCCGCGGAGCTTCAGCTCCGCCTTGTGCACGCGGTCTAGGAAG TCCACGTACGGCAGGTCCGTCGTGAACACCGTGCCCGGGATGAAATTCAGCTCGCCCAGCAGCG CCTCCACGTCCTGGAAAAAAAAATCGAAACTAATTATTTAAACTCCTAGTTTAGAATGATCTAC TTCGTCTTTCTTTTTCATCACTTGCGTATAGTACTATACTAGTGGTAATAGCTAGTACTGCGTA ATTAATTGATTAATTAAGTGGTCGATAAAATAGTGCATGTTTTGTCAGTGGGACTAAGATCTAG ACAGCTACTAGTACGTTTGCAAGATCCACCGATCCACTGTGCTCAGCATGTGGGTGCAATCATA CAATCATCAAGGGCTTGGATTACACTAGAGAGCTTGACTAAACCGCCCATAACTAACTAAAAAC ATGGCCCAATGATTGACTAGGTGCCCTGCCATTAGAGTACTAAACTACTCCAGTAGGAATAATT AAGAAATTTCGTCATAAACAAATGGGCGGTCAGGGGGGGAGGCAGAGAATTATTGGTGAATCAG GAAAGACGGCGCCGGCCGCGCGATCGCGCCGGCACGACGGACACGCACCTGGTCGACGGTTACG GCGGTGGAGTCGTCGTAATTCTTGGTGACCTCCAGGCAGTAGAGAACCCCGGAGTGGTGCTTGA GCGAGCTGAGCTTGACCGGGTTCTGCGGCGAGAAGAAGGACGACCTCCAGTTGTTGATTAGGCC CTCGGCGGCGACGACGAAGCCCTCCACGTAGTCGAACCGCCGGCCGCCGTGCTGCAGGGAGATG AGCCGCTCCTGGTCCGCGGTGAACTCGGTGAAGTTGGAGTAGAGCGCCCGGATCCAGCGAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.278838218

Conservation Information
Conserved circRNAs	NA
PMCS	0.278838218

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32789905-32790652(-)
						
						Potential amino acid sequence
						
							MWEVPHPWLNLFVPASRIADFDRGVFRGVLGSRTAGGPILIYPMNRHKFAGSGRSTPTSPSSPR
TRSGSSPCSTAAGGSTTWRASSSPPRA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32789905-32790652(-)
Potential amino acid sequence	MWEVPHPWLNLFVPASRIADFDRGVFRGVLGSRTAGGPILIYPMNRHKFAGSGRSTPTSPSSPR TRSGSSPCSTAAGGSTTWRASSSPPRA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0775400_circ_g.1

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