Plant Circular RNA Database

Detail information of Zm00001d033155_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d033155_circ_g.2
					
						ID in PlantcircBase
						zma_circ_006854
					
						Alias
						zma_circ_0000475
					
						Organism
						
							Zea mays						
					
						Position
						
							chr1: 252351406-252357238  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d033155						
					
						Parent gene annotation
						
							DUF21 domain-containing protein  chloroplastic
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d033155_circ_g.1 Zm00001d033155_circ_g.3 Zm00001d033155_circ_g.4 Zm00001d033155_circ_g.5 Zm00001d033155_circ_g.6 Zm00001d033155_circ_g.7 Zm00001d033155_circ_g.8 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d033155_T003:3
Zm00001d033155_T001:3
Zm00001d033155_T002:3

General Information
CircRNA Name	Zm00001d033155_circ_g.2
ID in PlantcircBase	zma_circ_006854
Alias	zma_circ_0000475
Organism	Zea mays
Position	chr1: 252351406-252357238 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	Zm00001d033155
Parent gene annotation	DUF21 domain-containing protein chloroplastic
Parent gene strand	-
Alternative splicing	Zm00001d033155_circ_g.1 Zm00001d033155_circ_g.3 Zm00001d033155_circ_g.4 Zm00001d033155_circ_g.5 Zm00001d033155_circ_g.6 Zm00001d033155_circ_g.7 Zm00001d033155_circ_g.8
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d033155_T003:3 Zm00001d033155_T001:3 Zm00001d033155_T002:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAGCTCTTCAGATAGGTGATCTATCGATGTGTTTGCATCAACATCAAATGTTCCATCCCCCCG
CCTTACTATATAGCCTGTTTTCTTCTGGATTTCCTCcagtagtacgatggtcttgcagttcctt
tcttgtcatcttttggcactagaactgcttgcatcttccaacaaggcactgttatcatccgagt
atgagagt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 594
Transcript exons: 252351406-252351702,252356629-252356808,252357122-252357
238
CAGTAGTACGATGGTCTTGCAGTTCCTTTCTTGTCATCTTTTGGCACTAGAACTGCTTGCATCT
TCCAACAAGGCACTGTTATCATCCGAGTATGAGAGTAACTCTGCAGGGCAACCATTCTCTGTTA
CGTCTGGACACTCGTCATCATTGGAGGAATCATCAGAGCCTTTCTTCTTCCGCTTGATGATCTT
CTTGGAAATGAGGCGGTTCACACCCTTGTTGTCGACATCAACACATTCACTGCTTATTGGCTTG
AATAGAACTTTGCCCACCTTCCTTGCATTAGCTTCCACTATCTCGAGTTCATAAGCTTGAGTTT
TTTCTCTATCATCTTGGCGATCAGGACCTTCCTCCTGATATTCACCGTTTTCTTCTCTGAAATT
CTTTTCAAGAATCACAAGCATTTTCCCACCTTCTTCTGGAATATAGCCAAAAGATGCACAGACA
AAACCAGACACCGTCTCATACTGATGTCCCTCTGGTATTTTGATACCAAGCTCTTCAGATAGGT
GATCTATCGATGTGTTTGCATCAACATCAAATGTTCCATCCCCCCGCCTTACTATATAGCCTGT
TTTCTTCTGGATTTCCTC
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CAAGCTCTTCAGATAGGTGATCTATCGATGTGTTTGCATCAACATCAAATGTTCCATCCCCCCG CCTTACTATATAGCCTGTTTTCTTCTGGATTTCCTCcagtagtacgatggtcttgcagttcctt tcttgtcatcttttggcactagaactgcttgcatcttccaacaaggcactgttatcatccgagt atgagagt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 594 Transcript exons: 252351406-252351702,252356629-252356808,252357122-252357 238 CAGTAGTACGATGGTCTTGCAGTTCCTTTCTTGTCATCTTTTGGCACTAGAACTGCTTGCATCT TCCAACAAGGCACTGTTATCATCCGAGTATGAGAGTAACTCTGCAGGGCAACCATTCTCTGTTA CGTCTGGACACTCGTCATCATTGGAGGAATCATCAGAGCCTTTCTTCTTCCGCTTGATGATCTT CTTGGAAATGAGGCGGTTCACACCCTTGTTGTCGACATCAACACATTCACTGCTTATTGGCTTG AATAGAACTTTGCCCACCTTCCTTGCATTAGCTTCCACTATCTCGAGTTCATAAGCTTGAGTTT TTTCTCTATCATCTTGGCGATCAGGACCTTCCTCCTGATATTCACCGTTTTCTTCTCTGAAATT CTTTTCAAGAATCACAAGCATTTTCCCACCTTCTTCTGGAATATAGCCAAAAGATGCACAGACA AAACCAGACACCGTCTCATACTGATGTCCCTCTGGTATTTTGATACCAAGCTCTTCAGATAGGT GATCTATCGATGTGTTTGCATCAACATCAAATGTTCCATCCCCCCGCCTTACTATATAGCCTGT TTTCTTCTGGATTTCCTC
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.064530975

Conservation Information
Conserved circRNAs	NA
PMCS	0.064530975

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							252357167-252351452(+)
252351526-252357210(-)
252351444-252357185(-)
						
						Potential amino acid sequence
						
							MCLHQHQMFHPPALLYSLFSSGFPPVVRWSCSSFLVIFWH*(+)
MVALQSYSHTRMITVPCWKMQAVLVPKDDKKGTARPSYYWRKSRRKQAI*(-)
MTRKELQDHRTTGGNPEENRLYSKAGGWNI*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	252357167-252351452(+) 252351526-252357210(-) 252351444-252357185(-)
Potential amino acid sequence	MCLHQHQMFHPPALLYSLFSSGFPPVVRWSCSSFLVIFWH(+) MVALQSYSHTRMITVPCWKMQAVLVPKDDKKGTARPSYYWRKSRRKQAI(-) MTRKELQDHRTTGGNPEENRLYSKAGGWNI*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d033155_circ_g.2

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