Plant Circular RNA Database

Detail information of AT3G13470_circ_g.14

			
						 General Information
					
						CircRNA Name
						AT3G13470_circ_g.14
					
						ID in PlantcircBase
						ath_circ_021478
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 4390903-4391594  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT3G13470						
					
						Parent gene annotation
						
							Chaperonin 60 subunit beta 2, chloroplastic
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT3G13470_circ_g.1 AT3G13470_circ_g.2 AT3G13470_circ_g.3 AT3G13470_circ_g.4 AT3G13470_circ_g.5 AT3G13470_circ_g.6 AT3G13470_circ_g.7 AT3G13470_circ_g.8 AT3G13470_circ_g.9 AT3G13470_circ_g.10 AT3G13470_circ_g.11 AT3G13470_circ_g.12 AT3G13470_circ_g.13 AT3G13470_circ_g.15 AT3G13470_circ_g.16 AT3G13470_circ_g.17 AT3G13470_circ_g.18 AT3G13470_circ_g.19 						
					
						Support reads
						10
					
						Tissues
						seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G13470.1:3

General Information
CircRNA Name	AT3G13470_circ_g.14
ID in PlantcircBase	ath_circ_021478
Alias	NA
Organism	Arabidpsis thaliana
Position	chr3: 4390903-4391594 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	AT3G13470
Parent gene annotation	Chaperonin 60 subunit beta 2, chloroplastic
Parent gene strand	+
Alternative splicing	AT3G13470_circ_g.1 AT3G13470_circ_g.2 AT3G13470_circ_g.3 AT3G13470_circ_g.4 AT3G13470_circ_g.5 AT3G13470_circ_g.6 AT3G13470_circ_g.7 AT3G13470_circ_g.8 AT3G13470_circ_g.9 AT3G13470_circ_g.10 AT3G13470_circ_g.11 AT3G13470_circ_g.12 AT3G13470_circ_g.13 AT3G13470_circ_g.15 AT3G13470_circ_g.16 AT3G13470_circ_g.17 AT3G13470_circ_g.18 AT3G13470_circ_g.19
Support reads	10
Tissues	seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G13470.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CTTGCGCTCTCCAAATCCAGGAG
 R: CAAGAAGCAGTGAACAAGCGTG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CTTGCGCTCTCCAAATCCAGGAG R: CAAGAAGCAGTGAACAAGCGTG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TAATACAACTGCGGTTGTGCAGCAAGCGGAGCAAGACTACGAGAAGGAAAAATTAAATGAGAGA
ATTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAGttgcttcttgttgacaagaaagtaacca
atgcaagggatcttgttggggttctagaggatgcgattagaggcggatacccaatcctcataat
tgcagaag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 483
Transcript exons: 4390903-4391137,4391255-4391424,4391517-4391594
TTGCTTCTTGTTGACAAGAAAGTAACCAATGCAAGGGATCTTGTTGGGGTTCTAGAGGATGCGA
TTAGAGGCGGATACCCAATCCTCATAATTGCAGAAGATATTGAACAAGAAGCCTTAGCTACTCT
TGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTCAAAGCTCCTGGATTTGGAGAG
CGCAAGAGCCAATACCTTGACGATATTGCCATTCTAACTGGAGCAACTGTGATCAGAGAGGAAG
TTGGTCTTTCTCTTGACAAAGCTGGAAAAGAGGTTCTGGGTAACGCTTCCAAGGTTGTCCTGAC
AAAGGAAATGACAACAATTGTGGGTGATGGGACCACACAAGAAGCAGTGAACAAGCGTGTTGTA
CAGATTAGGAATCTTATTGAGCAAGCGGAGCAAGACTACGAGAAGGAAAAATTAAATGAGAGAA
TTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAG
						
						Genomic sequence
						
							TTGCTTCTTGTTGACAAGAAAGTAACCAATGCAAGGGATCTTGTTGGGGTTCTAGAGGATGCGA
TTAGAGGCGGATACCCAATCCTCATAATTGCAGAAGATATTGAACAAGAAGCCTTAGCTACTCT
TGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTCAAAGCTCCTGGATTTGGAGAG
CGCAAGAGCCAATACCTTGACGATATTGCCATTCTAACTGGAGGTAGGTCATGTTTTTATAACA
AAAAACCTATATGTGGGTATCAATCAATGTGCCCTCCTGATTTGGAATCATTTGGCTGTTAAAA
CCTTACCGTGTTGCTTCTGTTTTTGTGTGCAGCAACTGTGATCAGAGAGGAAGTTGGTCTTTCT
CTTGACAAAGCTGGAAAAGAGGTTCTGGGTAACGCTTCCAAGGTTGTCCTGACAAAGGAAATGA
CAACAATTGTGGGTGATGGGACCACACAAGAAGCAGTGAACAAGCGTGTTGTACAGATTAGGAA
TCTTATTGAGGTATATGTTTGAGTCAAACCGCATAAGAGTCTGTGACAGCTACTTTTTTTTGTA
TAAAGTTTGTTTATGCTAATACAACTGCGGTTGTGCAGCAAGCGGAGCAAGACTACGAGAAGGA
AAAATTAAATGAGAGAATTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAG

Sequences
Splice junction sequence	TAATACAACTGCGGTTGTGCAGCAAGCGGAGCAAGACTACGAGAAGGAAAAATTAAATGAGAGA ATTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAGttgcttcttgttgacaagaaagtaacca atgcaagggatcttgttggggttctagaggatgcgattagaggcggatacccaatcctcataat tgcagaag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 483 Transcript exons: 4390903-4391137,4391255-4391424,4391517-4391594 TTGCTTCTTGTTGACAAGAAAGTAACCAATGCAAGGGATCTTGTTGGGGTTCTAGAGGATGCGA TTAGAGGCGGATACCCAATCCTCATAATTGCAGAAGATATTGAACAAGAAGCCTTAGCTACTCT TGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTCAAAGCTCCTGGATTTGGAGAG CGCAAGAGCCAATACCTTGACGATATTGCCATTCTAACTGGAGCAACTGTGATCAGAGAGGAAG TTGGTCTTTCTCTTGACAAAGCTGGAAAAGAGGTTCTGGGTAACGCTTCCAAGGTTGTCCTGAC AAAGGAAATGACAACAATTGTGGGTGATGGGACCACACAAGAAGCAGTGAACAAGCGTGTTGTA CAGATTAGGAATCTTATTGAGCAAGCGGAGCAAGACTACGAGAAGGAAAAATTAAATGAGAGAA TTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAG
Genomic sequence	TTGCTTCTTGTTGACAAGAAAGTAACCAATGCAAGGGATCTTGTTGGGGTTCTAGAGGATGCGA TTAGAGGCGGATACCCAATCCTCATAATTGCAGAAGATATTGAACAAGAAGCCTTAGCTACTCT TGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTCAAAGCTCCTGGATTTGGAGAG CGCAAGAGCCAATACCTTGACGATATTGCCATTCTAACTGGAGGTAGGTCATGTTTTTATAACA AAAAACCTATATGTGGGTATCAATCAATGTGCCCTCCTGATTTGGAATCATTTGGCTGTTAAAA CCTTACCGTGTTGCTTCTGTTTTTGTGTGCAGCAACTGTGATCAGAGAGGAAGTTGGTCTTTCT CTTGACAAAGCTGGAAAAGAGGTTCTGGGTAACGCTTCCAAGGTTGTCCTGACAAAGGAAATGA CAACAATTGTGGGTGATGGGACCACACAAGAAGCAGTGAACAAGCGTGTTGTACAGATTAGGAA TCTTATTGAGGTATATGTTTGAGTCAAACCGCATAAGAGTCTGTGACAGCTACTTTTTTTTGTA TAAAGTTTGTTTATGCTAATACAACTGCGGTTGTGCAGCAAGCGGAGCAAGACTACGAGAAGGA AAAATTAAATGAGAGAATTGCGAAGCTCTCTGGTGGAGTTGCTGTAATTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.328559915

Conservation Information
Conserved circRNAs	NA
PMCS	0.328559915

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4391347-4391591(+)
						
						Potential amino acid sequence
						
							MTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQLLLVDKKVTNAR
DLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAIL
TGATVIREEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQD
YEKEKLNERIAKLSGGVAVIQLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQEALATL
VVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLT
KEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQLLLVDKKVTN
ARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIA
ILTGATVIREEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAE
QDYEKEKLNERIAKLSGGVAVIQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	Y
Potential coding position	4391347-4391591(+)
Potential amino acid sequence	MTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQLLLVDKKVTNAR DLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAIL TGATVIREEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQD YEKEKLNERIAKLSGGVAVIQLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQEALATL VVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLT KEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQLLLVDKKVTN ARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIA ILTGATVIREEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAE QDYEKEKLNERIAKLSGGVAVIQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT3G13470_circ_g.14

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