Plant Circular RNA Database

Detail information of Os01g0624000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os01g0624000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_002756
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 24919361-24919832  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os01g0624000						
					
						Parent gene annotation
						
							Neutral/alkaline nonlysosomal ceramidase family protein. (Os01t0
624000-01);Neutral/alkaline nonlysosomal ceramidase family prote
in. (Os01t0624000-02);Similar to Neutral ceramidase. (Os01t06240
00-03);Similar to Neutral ceramidase. (Os01t0624000-04)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						24
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0624000-02:2
Os01t0624000-04:2
Os01t0624000-01:2
Os01t0624000-03:1

General Information
CircRNA Name	Os01g0624000_circ_g.2
ID in PlantcircBase	osa_circ_002756
Alias	NA
Organism	Oryza sativa
Position	chr1: 24919361-24919832 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os01g0624000
Parent gene annotation	Neutral/alkaline nonlysosomal ceramidase family protein. (Os01t0 624000-01);Neutral/alkaline nonlysosomal ceramidase family prote in. (Os01t0624000-02);Similar to Neutral ceramidase. (Os01t06240 00-03);Similar to Neutral ceramidase. (Os01t0624000-04)
Parent gene strand	-
Alternative splicing	NA
Support reads	24
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0624000-02:2 Os01t0624000-04:2 Os01t0624000-01:2 Os01t0624000-03:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTTGCCTTGTATTTCTTCAGAAGCTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTC
CCAATAACACGGGTACTTTCAAACTCATCAGGGTATcgcccaatcatatggttccttcatttca
ccagtgtctagcaatattggttttggagaatgacattcaacttggtccttccctggtgtcttta
acaagttc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 397
Transcript exons: 24919361-24919486,24919562-24919832
CGCCCAATCATATGGTTCCTTCATTTCACCAGTGTCTAGCAATATTGGTTTTGGAGAATGACAT
TCAACTTGGTCCTTCCCTGGTGTCTTTAACAAGTTCCTTACTAACTTCCAGAAAGGGTTTCCCT
TGACATCTCCTTGTCTGAAATCAAAAGCTCCAGGGCCATCTGTGGTTCCAGCAGCAAAAGCAAA
GCCCATTGCTGCTGGGCATGTTTTCACGGTCTGCTGACCGCCTGCACTTGTAGAAACTTTAACT
TCAAGTTTTGAGAAATCTAAATAAGTGTGCCTATAGTCAATTTTGCCTTGTATTTCTTCAGAAG
CTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTCCCAATAACACGGGTACTTTCAAA
CTCATCAGGGTAT
						
						Genomic sequence
						
							CGCCCAATCATATGGTTCCTTCATTTCACCAGTGTCTAGCAATATTGGTTTTGGAGAATGACAT
TCAACTTGGTCCTTCCCTGGTGTCTTTAACAAGTTCCTTACTAACTTCCAGAAAGGGTTTCCCT
GGTAAGAGATATATAAACCAGTGAGTAGCATGTTACCCAAATTTTGAAGCTACTATGTATTGAC
ATGCTAAACCTTGACATCTCCTTGTCTGAAATCAAAAGCTCCAGGGCCATCTGTGGTTCCAGCA
GCAAAAGCAAAGCCCATTGCTGCTGGGCATGTTTTCACGGTCTGCTGACCGCCTGCACTTGTAG
AAACTTTAACTTCAAGTTTTGAGAAATCTAAATAAGTGTGCCTATAGTCAATTTTGCCTTGTAT
TTCTTCAGAAGCTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTCCCAATAACACGG
GTACTTTCAAACTCATCAGGGTAT

Sequences
Splice junction sequence	TTTGCCTTGTATTTCTTCAGAAGCTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTC CCAATAACACGGGTACTTTCAAACTCATCAGGGTATcgcccaatcatatggttccttcatttca ccagtgtctagcaatattggttttggagaatgacattcaacttggtccttccctggtgtcttta acaagttc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 397 Transcript exons: 24919361-24919486,24919562-24919832 CGCCCAATCATATGGTTCCTTCATTTCACCAGTGTCTAGCAATATTGGTTTTGGAGAATGACAT TCAACTTGGTCCTTCCCTGGTGTCTTTAACAAGTTCCTTACTAACTTCCAGAAAGGGTTTCCCT TGACATCTCCTTGTCTGAAATCAAAAGCTCCAGGGCCATCTGTGGTTCCAGCAGCAAAAGCAAA GCCCATTGCTGCTGGGCATGTTTTCACGGTCTGCTGACCGCCTGCACTTGTAGAAACTTTAACT TCAAGTTTTGAGAAATCTAAATAAGTGTGCCTATAGTCAATTTTGCCTTGTATTTCTTCAGAAG CTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTCCCAATAACACGGGTACTTTCAAA CTCATCAGGGTAT
Genomic sequence	CGCCCAATCATATGGTTCCTTCATTTCACCAGTGTCTAGCAATATTGGTTTTGGAGAATGACAT TCAACTTGGTCCTTCCCTGGTGTCTTTAACAAGTTCCTTACTAACTTCCAGAAAGGGTTTCCCT GGTAAGAGATATATAAACCAGTGAGTAGCATGTTACCCAAATTTTGAAGCTACTATGTATTGAC ATGCTAAACCTTGACATCTCCTTGTCTGAAATCAAAAGCTCCAGGGCCATCTGTGGTTCCAGCA GCAAAAGCAAAGCCCATTGCTGCTGGGCATGTTTTCACGGTCTGCTGACCGCCTGCACTTGTAG AAACTTTAACTTCAAGTTTTGAGAAATCTAAATAAGTGTGCCTATAGTCAATTTTGCCTTGTAT TTCTTCAGAAGCTGAATCAAAGAGATCTCTAGCCTTTAGAAATTGCCTATTCCCAATAACACGG GTACTTTCAAACTCATCAGGGTAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_010360 						
					
						PMCS
						0.345283969

Conservation Information
Conserved circRNAs	osi_circ_010360
PMCS	0.345283969

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24919635-24919826(-)
24919373-24919826(-)
						
						Potential amino acid sequence
						
							MGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYD
WAIP*(-)
MIGRYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTV
KTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGE
MKEPYDWAIP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24919635-24919826(-) 24919373-24919826(-)
Potential amino acid sequence	MGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYD WAIP(-) MIGRYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTV KTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGE MKEPYDWAIP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0624000_circ_g.2

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