Plant Circular RNA Database

Detail information of GLYMA_06G222100_circ_g.4

			
						 General Information
					
						CircRNA Name
						GLYMA_06G222100_circ_g.4
					
						ID in PlantcircBase
						gma_circ_001523
					
						Alias
						Gm06circRNA625, gma-circRNA0793
					
						Organism
						
							Glycine max						
					
						Position
						
							chr6: 28899033-28900912  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Tophat, CIRI, find_circ
					
						Parent gene
						
							GLYMA_06G222100						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							GLYMA_06G222100_circ_g.2 GLYMA_06G222100_circ_g.3 GLYMA_06G222100_circ_g.5 GLYMA_06G222100_circ_g.6 GLYMA_06G222100_circ_g.7 GLYMA_06G222100_circ_g.8 GLYMA_06G222100_circ_g.9 						
					
						Support reads
						NA
					
						Tissues
						root, stem, flower bud
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRH54968:7
KRH54967:7

General Information
CircRNA Name	GLYMA_06G222100_circ_g.4
ID in PlantcircBase	gma_circ_001523
Alias	Gm06circRNA625, gma-circRNA0793
Organism	Glycine max
Position	chr6: 28899033-28900912 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	Tophat, CIRI, find_circ
Parent gene	GLYMA_06G222100
Parent gene annotation	hypothetical protein
Parent gene strand	-
Alternative splicing	GLYMA_06G222100_circ_g.2 GLYMA_06G222100_circ_g.3 GLYMA_06G222100_circ_g.5 GLYMA_06G222100_circ_g.6 GLYMA_06G222100_circ_g.7 GLYMA_06G222100_circ_g.8 GLYMA_06G222100_circ_g.9
Support reads	NA
Tissues	root, stem, flower bud
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRH54968:7 KRH54967:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CAGGCCATGCCTCCATTTTTG
 R: CTGTCCGCTCAATAGGTGTGT
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CAGGCCATGCCTCCATTTTTG R: CTGTCCGCTCAATAGGTGTGT PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGATGGAGGATTGCTGAACTTTTGGCTGAAAATCATATCTGACATACTCCAACAGGGCCACTAT
TTGATTATGATCATGGGAGAGTATAATATCTAATGGcttactcttgctgacagttcttatagca
gcattttcagaagtatcatgaagcaaaaatggaggcatggcctggcccccaccaatgtcattaa
gaccatgg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 834
Transcript exons: 28899033-28899236,28899752-28899829,28899940-28900026,28
900152-28900233,28900319-28900431,28900525-28900675,2890
0794-28900912
CTTACTCTTGCTGACAGTTCTTATAGCAGCATTTTCAGAAGTATCATGAAGCAAAAATGGAGGC
ATGGCCTGGCCCCCACCAATGTCATTAAGACCATGGGCAAAAAGATAAGAAAGGATTGTCTGAC
ATGCTTCTCTATGATTGGAGCTCCTAACATCACCAGCATGTAGTTCAACCGCTAAAAGTTTCAA
AAGCCATGCTCTCTGATGAAGGGAACTGTTTCGAAGTGACTGGTTGCTATTTCGTTTAGGAAGT
GGGGCAATACCAATTGTATCAAGGTGCTTAACAAAGAATAGGTACTTTTTATTTGACAACAGAT
CCATTGTTGGTCCACTTGTTAGTGGATCTACACACAATTCATAAAGCAGCTGAAAACCAAATTC
ATGAAGTAATGCATTTACACCAGGCTTCAAAAGCTTTTCCAGGATATCAAGAATAACCTTCATG
CAACTGTAATAAAATTTAGGCTGTAAAACTGTCCGCTCAATAGGTGTGTCTAGATCAAATTTAA
GCAGTAAATGAGTGATATTAGGGGCTGGCCGGCTGATATTGTCAATTAGAAGTTGCATAATAAG
AATGCCTGGATCATCATTGTTATTTTCAAGATTCTGAAGCTCTTCTGACCGTAACTCAAGGCAG
GCTGCATAATCTTCTATCAAGCTGTTTGATGCATTAGATTTTAGCAATAGTTGAACAAGCCCAA
CCATGCGAGAGCTTAAAATACTCATGATTTTGATGGAGGATTGCTGAACTTTTGGCTGAAAATC
ATATCTGACATACTCCAACAGGGCCACTATTTGATTATGATCATGGGAGAGTATAATATCTAAT
GG
						
					

						Genomic sequence
						
							CTTACTCTTGCTGACAGTTCTTATAGCAGCATTTTCAGAAGTATCATGAAGCAAAAATGGAGGC
ATGGCCTGGCCCCCACCAATGTCATTAAGACCATGGGCAAAAAGATAAGAAAGGATTGTCTGAC
ATGCTTCTCTATGATTGGAGCTCCTAACATCACCAGCATGTAGTTCAACCGCTAAAAGTTTCAA
AAGCCATGCTCTCTGAGAAAGCAAGGGAACAAAAACCTATATTAAACCCAACCTCATTGCATAC
TGTATACTGCATAGTTTGAAAGTAATACACCAATCCTATTCCTTCCCAGAAATTGACAGCATTG
AGACAAAAATGTTAAATACAGACATAAATAACGTAAACATGAAACACTACATGCATAAGACGTT
TAATGAAGAACCATTGGAGATAATTTTTATGCACTTCTACACTTTAAAAGATCTGAAAATAAAA
AACAATGAAAATAATTATAGTTTGAATTTGGAACGCTGTTTACTTGTTATTGTTATCAATAACA
AAATATCAAACTTAAGGATTATATTTCAACATGCTGACTAGGTGCCCCACTAGTTTTAATAGAA
TTGGTATTAACTATCAAACATAATTTTGAAAGAACCACTCACTTATCATTCTGATTACACTACA
GCCCCTTTTGTTTTGAACTTTAGACCTTTTATAAGTAAAGGTTGAGATGGTTTAGAAGGAGAAA
GTAGGGAAAAGCAACCTGATGAAGGGAACTGTTTCGAAGTGACTGGTTGCTATTTCGTTTAGGA
AGTGGGGCAATACCAATTGTATCAAGGTGCTGCATTTTAGCAAGGAAAAGAGTTTGAGAGATAT
ATCAATTCATTGAATTATATAATTATAAACAACTAAAAGTAGAAAAGGTGCTCAAATAAATCAA
AAACTGTTTACCTTAACAAAGAATAGGTACTTTTTATTTGACAACAGATCCATTGTTGGTCCAC
TTGTTAGTGGATCTACACACAATTCATAAAGCAGCTGCATCAAGCAAAAAGTCAAGATTCCTTT
TAGAAAAAGCATAAATGACTGGGCAAAATACAAAAAATCTAACTCTCTTTACTATATTGCATAC
ATAGGTTAGCAGTAGATCAAAAATGTCTGACCTGAAAACCAAATTCATGAAGTAATGCATTTAC
ACCAGGCTTCAAAAGCTTTTCCAGGATATCAAGAATAACCTTCATGCAACTGTAGAATGCAGAT
AACATTATAAGGGCACAGTTGTATTTTCAAGAATCAAGATATTAAGCACCATCAAATAATAGAC
AAAAACCTGTAATAAAATTTAGGCTGTAAAACTGTCCGCTCAATAGGTGTGTCTAGATCAAATT
TAAGCAGTAAATGAGTGATATTAGGGGCTGGCCGGCTGATATTGTCAATTAGAAGCTGAAAGCA
TATAGAAGGAGATGTAAATACAATCTTAGGAAGATTATACACAAGAAGAAACAAAATGAAAAGG
TGACAAGAATGAAGACAAACTTGCATAATAAGAATGCCTGGATCATCATTGTTATTTTCAAGAT
TCTGAAGCTCTTCTGACCGTAACTCAAGGCAGGCTGCATAATCTTCTATCAAGCTGTTTGATGC
ATTAGATTTTAGCAATAGTTGAACAAGCCCAACCATGCGAGAGCTGTCACTCAATGAAAAAAAA
TATATATATCAAATACAGATTGAGCATGATATTCATCAGTTAGAAGAACATATCAATTTACAAA
TTGAGGATAAAAAATGGAAAGGAATTAAAATGCCTTAAAATACTCATGATTTTGATGGAGGATT
GCTGAACTTTTGGCTGAAAATCATATCTGACATACTCCAACAGGGCCACTATTTGATTATGATC
ATGGGAGAGTATAATATCTAATGG
						
					

			
		

Sequences
Splice junction sequence	TGATGGAGGATTGCTGAACTTTTGGCTGAAAATCATATCTGACATACTCCAACAGGGCCACTAT TTGATTATGATCATGGGAGAGTATAATATCTAATGGcttactcttgctgacagttcttatagca gcattttcagaagtatcatgaagcaaaaatggaggcatggcctggcccccaccaatgtcattaa gaccatgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 834 Transcript exons: 28899033-28899236,28899752-28899829,28899940-28900026,28 900152-28900233,28900319-28900431,28900525-28900675,2890 0794-28900912 CTTACTCTTGCTGACAGTTCTTATAGCAGCATTTTCAGAAGTATCATGAAGCAAAAATGGAGGC ATGGCCTGGCCCCCACCAATGTCATTAAGACCATGGGCAAAAAGATAAGAAAGGATTGTCTGAC ATGCTTCTCTATGATTGGAGCTCCTAACATCACCAGCATGTAGTTCAACCGCTAAAAGTTTCAA AAGCCATGCTCTCTGATGAAGGGAACTGTTTCGAAGTGACTGGTTGCTATTTCGTTTAGGAAGT GGGGCAATACCAATTGTATCAAGGTGCTTAACAAAGAATAGGTACTTTTTATTTGACAACAGAT CCATTGTTGGTCCACTTGTTAGTGGATCTACACACAATTCATAAAGCAGCTGAAAACCAAATTC ATGAAGTAATGCATTTACACCAGGCTTCAAAAGCTTTTCCAGGATATCAAGAATAACCTTCATG CAACTGTAATAAAATTTAGGCTGTAAAACTGTCCGCTCAATAGGTGTGTCTAGATCAAATTTAA GCAGTAAATGAGTGATATTAGGGGCTGGCCGGCTGATATTGTCAATTAGAAGTTGCATAATAAG AATGCCTGGATCATCATTGTTATTTTCAAGATTCTGAAGCTCTTCTGACCGTAACTCAAGGCAG GCTGCATAATCTTCTATCAAGCTGTTTGATGCATTAGATTTTAGCAATAGTTGAACAAGCCCAA CCATGCGAGAGCTTAAAATACTCATGATTTTGATGGAGGATTGCTGAACTTTTGGCTGAAAATC ATATCTGACATACTCCAACAGGGCCACTATTTGATTATGATCATGGGAGAGTATAATATCTAAT GG
Genomic sequence	CTTACTCTTGCTGACAGTTCTTATAGCAGCATTTTCAGAAGTATCATGAAGCAAAAATGGAGGC ATGGCCTGGCCCCCACCAATGTCATTAAGACCATGGGCAAAAAGATAAGAAAGGATTGTCTGAC ATGCTTCTCTATGATTGGAGCTCCTAACATCACCAGCATGTAGTTCAACCGCTAAAAGTTTCAA AAGCCATGCTCTCTGAGAAAGCAAGGGAACAAAAACCTATATTAAACCCAACCTCATTGCATAC TGTATACTGCATAGTTTGAAAGTAATACACCAATCCTATTCCTTCCCAGAAATTGACAGCATTG AGACAAAAATGTTAAATACAGACATAAATAACGTAAACATGAAACACTACATGCATAAGACGTT TAATGAAGAACCATTGGAGATAATTTTTATGCACTTCTACACTTTAAAAGATCTGAAAATAAAA AACAATGAAAATAATTATAGTTTGAATTTGGAACGCTGTTTACTTGTTATTGTTATCAATAACA AAATATCAAACTTAAGGATTATATTTCAACATGCTGACTAGGTGCCCCACTAGTTTTAATAGAA TTGGTATTAACTATCAAACATAATTTTGAAAGAACCACTCACTTATCATTCTGATTACACTACA GCCCCTTTTGTTTTGAACTTTAGACCTTTTATAAGTAAAGGTTGAGATGGTTTAGAAGGAGAAA GTAGGGAAAAGCAACCTGATGAAGGGAACTGTTTCGAAGTGACTGGTTGCTATTTCGTTTAGGA AGTGGGGCAATACCAATTGTATCAAGGTGCTGCATTTTAGCAAGGAAAAGAGTTTGAGAGATAT ATCAATTCATTGAATTATATAATTATAAACAACTAAAAGTAGAAAAGGTGCTCAAATAAATCAA AAACTGTTTACCTTAACAAAGAATAGGTACTTTTTATTTGACAACAGATCCATTGTTGGTCCAC TTGTTAGTGGATCTACACACAATTCATAAAGCAGCTGCATCAAGCAAAAAGTCAAGATTCCTTT TAGAAAAAGCATAAATGACTGGGCAAAATACAAAAAATCTAACTCTCTTTACTATATTGCATAC ATAGGTTAGCAGTAGATCAAAAATGTCTGACCTGAAAACCAAATTCATGAAGTAATGCATTTAC ACCAGGCTTCAAAAGCTTTTCCAGGATATCAAGAATAACCTTCATGCAACTGTAGAATGCAGAT AACATTATAAGGGCACAGTTGTATTTTCAAGAATCAAGATATTAAGCACCATCAAATAATAGAC AAAAACCTGTAATAAAATTTAGGCTGTAAAACTGTCCGCTCAATAGGTGTGTCTAGATCAAATT TAAGCAGTAAATGAGTGATATTAGGGGCTGGCCGGCTGATATTGTCAATTAGAAGCTGAAAGCA TATAGAAGGAGATGTAAATACAATCTTAGGAAGATTATACACAAGAAGAAACAAAATGAAAAGG TGACAAGAATGAAGACAAACTTGCATAATAAGAATGCCTGGATCATCATTGTTATTTTCAAGAT TCTGAAGCTCTTCTGACCGTAACTCAAGGCAGGCTGCATAATCTTCTATCAAGCTGTTTGATGC ATTAGATTTTAGCAATAGTTGAACAAGCCCAACCATGCGAGAGCTGTCACTCAATGAAAAAAAA TATATATATCAAATACAGATTGAGCATGATATTCATCAGTTAGAAGAACATATCAATTTACAAA TTGAGGATAAAAAATGGAAAGGAATTAAAATGCCTTAAAATACTCATGATTTTGATGGAGGATT GCTGAACTTTTGGCTGAAAATCATATCTGACATACTCCAACAGGGCCACTATTTGATTATGATC ATGGGAGAGTATAATATCTAATGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.216622394

Conservation Information
Conserved circRNAs	NA
PMCS	0.216622394

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							28900809-28899035(-)
						
						Potential amino acid sequence
						
							MSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNISRP
APNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELCVD
PLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAGDV
RSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENAAIRTVSKSKPLDIILSHDHNQI
VALLEYVRYDFQPKVQQSSIKIMSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNL
ENNNDDPGILIMQLLIDNISRPAPNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLL
KPGVNALLHEFGFQLLYELCVDPLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRN
SSLHQRAWLLKLLAVELHAGDVRSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENA
AIRTVSKSKPLDIILSHDHNQIVALLEYVRYDFQPKVQQSSIKIMSILSSRMVGLVQLLLKSNA
SNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNISRPAPNITHLLLKFDLDTPIERT
VLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELCVDPLTSGPTMDLLSNKKYLFFV
KHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAGDVRSSNHREACQTILSYLFAHG
LNDIGGGQAMPPFLLHDTSENAAIRTVSKSKPLDIILSHDHNQIVALLEYVRYDFQPKVQQSSI
KIMSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNIS
RPAPNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELC
VDPLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAG
DVRSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENAAIRTVSKSK(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						cytoplasmic male sterility (acting as miRNA sponges)

Functional Information
Coding potential	Y
Potential coding position	28900809-28899035(-)
Potential amino acid sequence	MSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNISRP APNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELCVD PLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAGDV RSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENAAIRTVSKSKPLDIILSHDHNQI VALLEYVRYDFQPKVQQSSIKIMSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNL ENNNDDPGILIMQLLIDNISRPAPNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLL KPGVNALLHEFGFQLLYELCVDPLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRN SSLHQRAWLLKLLAVELHAGDVRSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENA AIRTVSKSKPLDIILSHDHNQIVALLEYVRYDFQPKVQQSSIKIMSILSSRMVGLVQLLLKSNA SNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNISRPAPNITHLLLKFDLDTPIERT VLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELCVDPLTSGPTMDLLSNKKYLFFV KHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAGDVRSSNHREACQTILSYLFAHG LNDIGGGQAMPPFLLHDTSENAAIRTVSKSKPLDIILSHDHNQIVALLEYVRYDFQPKVQQSSI KIMSILSSRMVGLVQLLLKSNASNSLIEDYAACLELRSEELQNLENNNDDPGILIMQLLIDNIS RPAPNITHLLLKFDLDTPIERTVLQPKFYYSCMKVILDILEKLLKPGVNALLHEFGFQLLYELC VDPLTSGPTMDLLSNKKYLFFVKHLDTIGIAPLPKRNSNQSLRNSSLHQRAWLLKLLAVELHAG DVRSSNHREACQTILSYLFAHGLNDIGGGQAMPPFLLHDTSENAAIRTVSKSK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	cytoplasmic male sterility (acting as miRNA sponges)

			
						 Other Information
					
						References
						Zhao et al., 2017a; Chen et al., 2018

circRNA

Detail information of GLYMA_06G222100_circ_g.4

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