Plant Circular RNA Database

Detail information of Os02g0761000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0761000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_016656
					
						Alias
						Os02circ27312
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 32041561-32043850  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl
					
						Parent gene
						
							Os02g0761000						
					
						Parent gene annotation
						
							Similar to Transcription factor MADS55. (Os02t0761000-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0761000_circ_g.1 Os02g0761000_circ_g.3 Os02g0761000_circ_g.4 						
					
						Support reads
						2/1
					
						Tissues
						leaf/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0761000-01:6

General Information
CircRNA Name	Os02g0761000_circ_g.2
ID in PlantcircBase	osa_circ_016656
Alias	Os02circ27312
Organism	Oryza sativa
Position	chr2: 32041561-32043850 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl
Parent gene	Os02g0761000
Parent gene annotation	Similar to Transcription factor MADS55. (Os02t0761000-01)
Parent gene strand	+
Alternative splicing	Os02g0761000_circ_g.1 Os02g0761000_circ_g.3 Os02g0761000_circ_g.4
Support reads	2/1
Tissues	leaf/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0761000-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CCGAAGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGTTCACAGTCGCAGGA
TAATGATGATGGCTCGGATGTATCCCTGAAATTAGGtatgaatgaaatcatcgacaagtacaac
acacattctaataatctggggaaagcagaacagccttcgctcgacttgaatgtatgtgaatcat
cttatcat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 480
Transcript exons: 32041561-32041639,32042315-32042376,32042500-32042599,32
042676-32042717,32043564-32043605,32043696-32043850
TATGAATGAAATCATCGACAAGTACAACACACATTCTAATAATCTGGGGAAAGCAGAACAGCCT
TCGCTCGACTTGAATTTAGAACATAGCAAGTATGCACATTTGAATGAGCAACTTGCGGAAGCTA
GTCTCCGGCTTAGACAAATGAGAGGTGAGGAGCTTGAGGGATTGAGCATTGATGAACTCCAGCA
GCTAGAGAAGAACCTGGAAGCTGGTCTGCACAGGGTGATGCTGACAAAGGATCAACAATTTATG
GAGCAGATCAGTGAACTCCAGCGGAAGAGTTCACAGCTGGCAGAGGAGAACATGCAACTCAGAA
ACCAGGTATCCCAGATATCACCAGCTGAGAAGCAAGTTGTTGATACTGAAAATTTTGTTACCGA
AGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGTTCACAGTCGCAGGATAAT
GATGATGGCTCGGATGTATCCCTGAAATTAGG
						
					

						Genomic sequence
						
							TATGAATGAAATCATCGACAAGTACAACACACATTCTAATAATCTGGGGAAAGCAGAACAGCCT
TCGCTCGACTTGAATGTATGTGAATCATCTTATCATACTGTGTTAATAGTAAATACTGTTCTTC
ATATATATTGCATCTTAATGTTGCCTAACTACATATAGTAGTATTAGATAATGGTTCAAAATGG
GTCATTTTATGTCCATGGCCTTAGGGTGGCACAATAGCAAAATATTCATATTTAGGTTTTCACA
TCTGGTAGCATTATGATCTTATTTCTGTACCCTAAAATTTTACAAATTAAACTTAAAAAAACAA
TCGCATATGTGGATCCTTTCAAAGTTCTCAGTATGCTCTAATGATGTCTGTTTTGTTGGTTGTG
TGTTAAATATGTAAATTATTTGTTTTAATTTTTTGTTGCTGGATTTTTTTTAAAAAAATTAATA
CTTGCAGAAACAATTTACATCTCTCACTTCCTCATATTTTTGGTGATACACTATAAATTAACAT
TATTAAATAATATAGTTCTCTATCTTTTTCTATTGTATCTTGAGAAAAATTCCCTGGAATCGGT
ACACTATAGCACAATACTAATTCTGTTTTTTCATGGATCATGAACTCAAATTTAGTACTGTTAG
TAGTAATTAGTGCTCAGGCATGATGTAATAGGTGCCTTAAACTTATGCAGATATATTTTCTAAA
TGCTACTTATGCAGATAGTTAATACTAACAGTTCAATCTTTTCTTTTCAGTTAGAACATAGCAA
GTATGCACATTTGAATGAGCAACTTGCGGAAGCTAGTCTCCGGCTTAGGTCAGTTCTTTTAACC
TTTTTTTTTTAAAAAAAATGTTATACTTGAGGCATTTGTTCTCAGATCCTTTATTCTTGAGGCA
TTTATTCTCAAATGTATATCATTGGTACATGAACAAATTGTAGACAAATGAGAGGTGAGGAGCT
TGAGGGATTGAGCATTGATGAACTCCAGCAGCTAGAGAAGAACCTGGAAGCTGGTCTGCACAGG
GTGATGCTGACAAAGGTTTGGTGATCATCACCCAGACTCATCACGTAGCTCGTACTATGAACAA
CTTATTGATTGCTATATGATTTTGCAGGATCAACAATTTATGGAGCAGATCAGTGAACTCCAGC
GGAAGGTACCACCTGTTGCTAGATTAACCCTGAATCATCAATGTAGCTTGGCAACATATAATTT
GTTTTTTCTTGCAAAAGTAGAACCTTTAAAGAAGACTGTGTTGCATACAAGTATCCGTTGCTGG
CTTATTTGTAGTTCATTCTTTCCAATACATCACTGTGTAAAAAGTTGATTTATAGTTCTCTCCA
ATACAGAGTGAAATCACTTATAATTTTACTGTTGCATCTTGCATGACTAATATATGCTGCCACC
TTCTGCCTTTTCTTGCAAGATGGCACATGCATCTGTTCAAGCAAATTTACTAAATCATCACCAC
CCTGGCCTTTTCCCCATGGATGAGTCGGAATTTTGGACTAAATTCCCTGCCCAACCCTTGGAAG
TCTGCGGGTTTTGTTCATGCCGGAAAAGTCCTTAGCGATTAAAAACAATTTCTTCTAGTCCTTA
TTGTTTCAATTTTACCAAATTATATTGGCGTTTTAAGTCATGTCCCTTCTTCCCCCAAATTGAA
ACATTGATTTTGACTATCTTGAAGATCAAATTAGAGGGTACCCATTGGATAAAATAACTAATTT
AAATGGAAATTGTACTTTAGTTTTTCTGTCCTGCATCATTTACCATTTGCCCATTTACATACAT
TTAAGTATTGTAGATGTTTCTTGCTGTGTTGGAAACTGATCAAACAGTACCATTTCTTGGGAAA
TGCAATCTTATAGTTAAAAAAAGAATCTACAATAGATTCTACCGCATCCAAAATGTTCTTGTGC
CTGCTTGTTTTTATTCAAACCCTTACCAATTGAAATAAGAGAACATTATAATTATGTGTTGCAT
ACTCTCTGTTGCACTGCAGAGTTCACAGCTGGCAGAGGAGAACATGCAACTCAGAAACCAGGTA
TAAATTTTACTTCTGTAAGCTCTTGGTTTGTTGAGGACTTAAGACCAGTCCATCTGAACACATT
CGTTTTCTTGGTACTGAATACAGGTATCCCAGATATCACCAGCTGAGAAGCAAGTTGTTGATAC
TGAAAATTTTGTTACCGAAGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGT
TCACAGTCGCAGGATAATGATGATGGCTCGGATGTATCCCTGAAATTAGG
						
					

			
		

Sequences
Splice junction sequence	CCGAAGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGTTCACAGTCGCAGGA TAATGATGATGGCTCGGATGTATCCCTGAAATTAGGtatgaatgaaatcatcgacaagtacaac acacattctaataatctggggaaagcagaacagccttcgctcgacttgaatgtatgtgaatcat cttatcat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 480 Transcript exons: 32041561-32041639,32042315-32042376,32042500-32042599,32 042676-32042717,32043564-32043605,32043696-32043850 TATGAATGAAATCATCGACAAGTACAACACACATTCTAATAATCTGGGGAAAGCAGAACAGCCT TCGCTCGACTTGAATTTAGAACATAGCAAGTATGCACATTTGAATGAGCAACTTGCGGAAGCTA GTCTCCGGCTTAGACAAATGAGAGGTGAGGAGCTTGAGGGATTGAGCATTGATGAACTCCAGCA GCTAGAGAAGAACCTGGAAGCTGGTCTGCACAGGGTGATGCTGACAAAGGATCAACAATTTATG GAGCAGATCAGTGAACTCCAGCGGAAGAGTTCACAGCTGGCAGAGGAGAACATGCAACTCAGAA ACCAGGTATCCCAGATATCACCAGCTGAGAAGCAAGTTGTTGATACTGAAAATTTTGTTACCGA AGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGTTCACAGTCGCAGGATAAT GATGATGGCTCGGATGTATCCCTGAAATTAGG
Genomic sequence	TATGAATGAAATCATCGACAAGTACAACACACATTCTAATAATCTGGGGAAAGCAGAACAGCCT TCGCTCGACTTGAATGTATGTGAATCATCTTATCATACTGTGTTAATAGTAAATACTGTTCTTC ATATATATTGCATCTTAATGTTGCCTAACTACATATAGTAGTATTAGATAATGGTTCAAAATGG GTCATTTTATGTCCATGGCCTTAGGGTGGCACAATAGCAAAATATTCATATTTAGGTTTTCACA TCTGGTAGCATTATGATCTTATTTCTGTACCCTAAAATTTTACAAATTAAACTTAAAAAAACAA TCGCATATGTGGATCCTTTCAAAGTTCTCAGTATGCTCTAATGATGTCTGTTTTGTTGGTTGTG TGTTAAATATGTAAATTATTTGTTTTAATTTTTTGTTGCTGGATTTTTTTTAAAAAAATTAATA CTTGCAGAAACAATTTACATCTCTCACTTCCTCATATTTTTGGTGATACACTATAAATTAACAT TATTAAATAATATAGTTCTCTATCTTTTTCTATTGTATCTTGAGAAAAATTCCCTGGAATCGGT ACACTATAGCACAATACTAATTCTGTTTTTTCATGGATCATGAACTCAAATTTAGTACTGTTAG TAGTAATTAGTGCTCAGGCATGATGTAATAGGTGCCTTAAACTTATGCAGATATATTTTCTAAA TGCTACTTATGCAGATAGTTAATACTAACAGTTCAATCTTTTCTTTTCAGTTAGAACATAGCAA GTATGCACATTTGAATGAGCAACTTGCGGAAGCTAGTCTCCGGCTTAGGTCAGTTCTTTTAACC TTTTTTTTTTAAAAAAAATGTTATACTTGAGGCATTTGTTCTCAGATCCTTTATTCTTGAGGCA TTTATTCTCAAATGTATATCATTGGTACATGAACAAATTGTAGACAAATGAGAGGTGAGGAGCT TGAGGGATTGAGCATTGATGAACTCCAGCAGCTAGAGAAGAACCTGGAAGCTGGTCTGCACAGG GTGATGCTGACAAAGGTTTGGTGATCATCACCCAGACTCATCACGTAGCTCGTACTATGAACAA CTTATTGATTGCTATATGATTTTGCAGGATCAACAATTTATGGAGCAGATCAGTGAACTCCAGC GGAAGGTACCACCTGTTGCTAGATTAACCCTGAATCATCAATGTAGCTTGGCAACATATAATTT GTTTTTTCTTGCAAAAGTAGAACCTTTAAAGAAGACTGTGTTGCATACAAGTATCCGTTGCTGG CTTATTTGTAGTTCATTCTTTCCAATACATCACTGTGTAAAAAGTTGATTTATAGTTCTCTCCA ATACAGAGTGAAATCACTTATAATTTTACTGTTGCATCTTGCATGACTAATATATGCTGCCACC TTCTGCCTTTTCTTGCAAGATGGCACATGCATCTGTTCAAGCAAATTTACTAAATCATCACCAC CCTGGCCTTTTCCCCATGGATGAGTCGGAATTTTGGACTAAATTCCCTGCCCAACCCTTGGAAG TCTGCGGGTTTTGTTCATGCCGGAAAAGTCCTTAGCGATTAAAAACAATTTCTTCTAGTCCTTA TTGTTTCAATTTTACCAAATTATATTGGCGTTTTAAGTCATGTCCCTTCTTCCCCCAAATTGAA ACATTGATTTTGACTATCTTGAAGATCAAATTAGAGGGTACCCATTGGATAAAATAACTAATTT AAATGGAAATTGTACTTTAGTTTTTCTGTCCTGCATCATTTACCATTTGCCCATTTACATACAT TTAAGTATTGTAGATGTTTCTTGCTGTGTTGGAAACTGATCAAACAGTACCATTTCTTGGGAAA TGCAATCTTATAGTTAAAAAAAGAATCTACAATAGATTCTACCGCATCCAAAATGTTCTTGTGC CTGCTTGTTTTTATTCAAACCCTTACCAATTGAAATAAGAGAACATTATAATTATGTGTTGCAT ACTCTCTGTTGCACTGCAGAGTTCACAGCTGGCAGAGGAGAACATGCAACTCAGAAACCAGGTA TAAATTTTACTTCTGTAAGCTCTTGGTTTGTTGAGGACTTAAGACCAGTCCATCTGAACACATT CGTTTTCTTGGTACTGAATACAGGTATCCCAGATATCACCAGCTGAGAAGCAAGTTGTTGATAC TGAAAATTTTGTTACCGAAGGACAGTCCTCTGAATCTGTGATGACTGCATTGCATTCTGGAAGT TCACAGTCGCAGGATAATGATGATGGCTCGGATGTATCCCTGAAATTAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012323 						
					
						PMCS
						0.312301567

Conservation Information
Conserved circRNAs	osi_circ_012323
PMCS	0.312301567

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32042537-32042680(-)
						
						Potential amino acid sequence
						
							MLNPSSSSPLICLSRRLASASCSFKCAYLLCSKFKSSEGCSAFPRLLECVLYLSMISFIPNFRD
TSEPSSLSCDCELPECNAVITDSEDCPSVTKFSVSTTCFSAGDIWDTWFLSCMFSSASCELFRW
SSLICSINC*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32042537-32042680(-)
Potential amino acid sequence	MLNPSSSSPLICLSRRLASASCSFKCAYLLCSKFKSSEGCSAFPRLLECVLYLSMISFIPNFRD TSEPSSLSCDCELPECNAVITDSEDCPSVTKFSVSTTCFSAGDIWDTWFLSCMFSSASCELFRW SSLICSINC*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0761000_circ_g.2

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