Plant Circular RNA Database

Detail information of Os05g0529000_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0529000_circ_g.2
					
						ID in PlantcircBase
						osa_circ_028611
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 26280039-26280691  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os05g0529000						
					
						Parent gene annotation
						
							Tonoplast-localized DUF502-containing protein, COV-like protein,
 Regulation of intracellular auxin transport (Os05t0529000-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0529000_circ_g.1 						
					
						Support reads
						23
					
						Tissues
						root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0529000-01:3

General Information
CircRNA Name	Os05g0529000_circ_g.2
ID in PlantcircBase	osa_circ_028611
Alias	NA
Organism	Oryza sativa
Position	chr5: 26280039-26280691 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os05g0529000
Parent gene annotation	Tonoplast-localized DUF502-containing protein, COV-like protein, Regulation of intracellular auxin transport (Os05t0529000-01)
Parent gene strand	-
Alternative splicing	Os05g0529000_circ_g.1
Support reads	23
Tissues	root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0529000-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATAATCCACTCACCAAGGCTAAGGACAGATGCTCCAACCCAAGATGACATGAAGACTCCAACCA
CAAATATGAAGGTAACAGAAGTGATAAAGCCAAGACcgatgccttcacggacagaaagatttgg
ccttataacatccttcgaatttaccatgaagatatcaccaatataaagatggttggttgggaca
tacacgca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 393
Transcript exons: 26280039-26280165,26280335-26280432,26280524-26280691
CGATGCCTTCACGGACAGAAAGATTTGGCCTTATAACATCCTTCGAATTTACCATGAAGATATC
ACCAATATAAAGATGGTTGGTTGGGACATACACGCAGTACAGTTCCTCTTGACCAGTATAACTC
TGGAGAGAGACTGATGATGTAATGAAACCAAATGCATATTCTCCAATACGAGGATGCCTTATGA
TGACCACTTCCTTGAATGCCTGTTTGTTCTGATCTGGTGATATTGCAGCACTTATTTGCTTGGA
TGCATTGTAGATATGACGGACAAGTGGCATGCGCTTAATAATCCACTCACCAAGGCTAAGGACA
GATGCTCCAACCCAAGATGACATGAAGACTCCAACCACAAATATGAAGGTAACAGAAGTGATAA
AGCCAAGAC
						
						Genomic sequence
						
							CGATGCCTTCACGGACAGAAAGATTTGGCCTTATAACATCCTTCGAATTTACCATGAAGATATC
ACCAATATAAAGATGGTTGGTTGGGACATACACGCAGTACAGTTCCTCTTGACCAGTATAACTC
TGTTACCACGTGATGATGAGATGAAATTAGGTACTGAAACCAGACATTTACTACATGCTAATAT
AAAAGCTGAACGGATATCAACAATTTTATTATTTTTGTACAAATGACATAAACATCTTAATGTA
ATGTAATCTCAAGCGAAATAATAGTTAATAGTCGTCTTACCTGGAGAGAGACTGATGATGTAAT
GAAACCAAATGCATATTCTCCAATACGAGGATGCCTTATGATGACCACTTCCTTGAATGCCTGT
TTGTTCTGATCTGTAGAAACCAGAGTTAATTAGAAATCAGCAGAGTTAATTTGAACCCAAGCTA
ATTTGCAGACATGCTATTAGTGCGACAGTAGGTTTACCTGGTGATATTGCAGCACTTATTTGCT
TGGATGCATTGTAGATATGACGGACAAGTGGCATGCGCTTAATAATCCACTCACCAAGGCTAAG
GACAGATGCTCCAACCCAAGATGACATGAAGACTCCAACCACAAATATGAAGGTAACAGAAGTG
ATAAAGCCAAGAC

Sequences
Splice junction sequence	ATAATCCACTCACCAAGGCTAAGGACAGATGCTCCAACCCAAGATGACATGAAGACTCCAACCA CAAATATGAAGGTAACAGAAGTGATAAAGCCAAGACcgatgccttcacggacagaaagatttgg ccttataacatccttcgaatttaccatgaagatatcaccaatataaagatggttggttgggaca tacacgca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 393 Transcript exons: 26280039-26280165,26280335-26280432,26280524-26280691 CGATGCCTTCACGGACAGAAAGATTTGGCCTTATAACATCCTTCGAATTTACCATGAAGATATC ACCAATATAAAGATGGTTGGTTGGGACATACACGCAGTACAGTTCCTCTTGACCAGTATAACTC TGGAGAGAGACTGATGATGTAATGAAACCAAATGCATATTCTCCAATACGAGGATGCCTTATGA TGACCACTTCCTTGAATGCCTGTTTGTTCTGATCTGGTGATATTGCAGCACTTATTTGCTTGGA TGCATTGTAGATATGACGGACAAGTGGCATGCGCTTAATAATCCACTCACCAAGGCTAAGGACA GATGCTCCAACCCAAGATGACATGAAGACTCCAACCACAAATATGAAGGTAACAGAAGTGATAA AGCCAAGAC
Genomic sequence	CGATGCCTTCACGGACAGAAAGATTTGGCCTTATAACATCCTTCGAATTTACCATGAAGATATC ACCAATATAAAGATGGTTGGTTGGGACATACACGCAGTACAGTTCCTCTTGACCAGTATAACTC TGTTACCACGTGATGATGAGATGAAATTAGGTACTGAAACCAGACATTTACTACATGCTAATAT AAAAGCTGAACGGATATCAACAATTTTATTATTTTTGTACAAATGACATAAACATCTTAATGTA ATGTAATCTCAAGCGAAATAATAGTTAATAGTCGTCTTACCTGGAGAGAGACTGATGATGTAAT GAAACCAAATGCATATTCTCCAATACGAGGATGCCTTATGATGACCACTTCCTTGAATGCCTGT TTGTTCTGATCTGTAGAAACCAGAGTTAATTAGAAATCAGCAGAGTTAATTTGAACCCAAGCTA ATTTGCAGACATGCTATTAGTGCGACAGTAGGTTTACCTGGTGATATTGCAGCACTTATTTGCT TGGATGCATTGTAGATATGACGGACAAGTGGCATGCGCTTAATAATCCACTCACCAAGGCTAAG GACAGATGCTCCAACCCAAGATGACATGAAGACTCCAACCACAAATATGAAGGTAACAGAAGTG ATAAAGCCAAGAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_015681 						
					
						PMCS
						0.255247039

Conservation Information
Conserved circRNAs	osi_circ_015681
PMCS	0.255247039

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26280584-26280109(+)
26280643-26280041(-)
						
						Potential amino acid sequence
						
							MRLIIHSPRLRTDAPTQDDMKTPTTNMKVTEVIKPRPMPSRTERFGLITSFEFTMKISPI*(+)
MSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIGEYAFGF
ITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSVTFIFVV
GVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIGEYA
FGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSVTFI
FVVGVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIG
EYAFGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSV
TFIFVVGVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHP
RIGEYAFGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGI(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26280584-26280109(+) 26280643-26280041(-)
Potential amino acid sequence	MRLIIHSPRLRTDAPTQDDMKTPTTNMKVTEVIKPRPMPSRTERFGLITSFEFTMKISPI*(+) MSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIGEYAFGF ITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSVTFIFVV GVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIGEYA FGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSVTFI FVVGVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHPRIG EYAFGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGIGLGFITSV TFIFVVGVFMSSWVGASVLSLGEWIIKRMPLVRHIYNASKQISAAISPDQNKQAFKEVVIIRHP RIGEYAFGFITSSVSLQSYTGQEELYCVYVPTNHLYIGDIFMVNSKDVIRPNLSVREGI(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0529000_circ_g.2

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