Plant Circular RNA Database

Detail information of orai.006G005300_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.006G005300_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000551
					
						Alias
						Chr06:1038608|1040542
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr06: 1038608-1040542  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.006G005300						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/12
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.006G005300.4:3
Gorai.006G005300.3:4
Gorai.006G005300.2:5
Gorai.006G005300.1:5

General Information
CircRNA Name	orai.006G005300_circ_g.1
ID in PlantcircBase	gra_circ_000551
Alias	Chr06:1038608\|1040542
Organism	Gossypium raimondii
Position	chrChr06: 1038608-1040542 JBrowse»
Reference genome	Graimondii_221
Type	ue-circRNA
Identification method	CIRI
Parent gene	Gorai.006G005300
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	2/12
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.006G005300.4:3 Gorai.006G005300.3:4 Gorai.006G005300.2:5 Gorai.006G005300.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GCTCTATCCAGAGGAGATGGAGCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAA
TATGGGATCATGCTGCTGGGTGTATTGTTGTGAGTGgctcacaagcactggttagttttgtgct
gcagcaggaatttcctgataacttctcattagttgctgaggaggtgaatctctgtacttgtgaa
taatagtt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 738
Transcript exons: 1038608-1038678,1038789-1038991,1039508-1039718,1039798-
1039896,1040389-1040542
GCTCACAAGCACTGGTTAGTTTTGTGCTGCAGCAGGAATTTCCTGATAACTTCTCATTAGTTGC
TGAGGAGGATTCTAAAGATCTTCGCAAGGATGGTGGCCAGGAAATAGTAGAGCGCATTACAAAA
CTTGTGAACGATTCTCTAACTATTGATGGATCATACAATGTTACTTTATCCACAGAAGATATTC
TCAGGGCCATTGACAATGGCAGATCTGAAGGTGGTTCCCAAGGTCGACACTGGGTTTTGGATCC
TATAGATGGTACTAAAGGTTTTCTGAGAGGAGATCAATATGCAATAGCATTGGCTTTGCTAGAT
GGAGGAAAAGTTGTCCTGGGTGTGCTGGCTTGTCCAAATCTTCCACTAACTTCCCTCAGTGATG
CTGGTCAGCATTCTCCAAATAATAAAGTTGGCTGCCTTTTCTTTGCTGTAGTAGGTGGTGGAAC
TTATATGCAGCCACTTGATGGTTCTTCGGCAGTAAAGGTGCAAGTAAGTGCTGTTGAAAATCCT
GAAGAAGCATCATTCTTTGAGTCTTATGAAGCAGCACACTCCATGCATGATTTATCTAGCTTGA
TTGCCCAAAAACTCGGCGTCAAAGCACCACCGGTTAGAATTGATAGCCAGGCGAAGTATGGTGC
TCTATCCAGAGGAGATGGAGCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAATA
TGGGATCATGCTGCTGGGTGTATTGTTGTGAGTG
						
					

						Genomic sequence
						
							GCTCACAAGCACTGGTTAGTTTTGTGCTGCAGCAGGAATTTCCTGATAACTTCTCATTAGTTGC
TGAGGAGGTGAATCTCTGTACTTGTGAATAATAGTTTCAGCAGCATGCATCAGCCATCTTTTTT
TTACATCATAGAATTCTTCTTTCCTTCTAAATAATGTTTTGCTTTCTCTTTAGGATTCTAAAGA
TCTTCGCAAGGATGGTGGCCAGGAAATAGTAGAGCGCATTACAAAACTTGTGAACGATTCTCTA
ACTATTGATGGATCATACAATGTTACTTTATCCACAGAAGATATTCTCAGGGCCATTGACAATG
GCAGATCTGAAGGTGGTTCCCAAGGTCGACACTGGGTTTTGGATCCTATAGATGGTACTAAAGG
GTGAGCTCTGAAACTTTATATTTGACATAAGTGATTTTGCTTTAGTCATAAAGAAGTTGGTGTC
TGCTATAATAATGATTCATGTGCACCGAGTGCATGTTTATGATATGCCTTATCCAACATGAGGT
AGTAACTTTCCTTTTTACTCCTTAACTCAGCAATGACTACTGCAGGGGGATGTTTAGGGGCATA
CCTTAACAGTACTATATGCAAGACAAACCATAATTCTGTCTTGGTCTTGCATAGCCAGGCCTTT
GAATTTAGTGCTTTAGAATTAAATGACTGATCATCAACGAACTTTCTCATGTATATAACTTTTG
TTTTATCTTTGTTACACATGTTATGTTTGGATTTCTTTGCAATTTAGTGTGAAAGAAATGGCTG
ATCTTTTTATTTTTGCATAATAATGATCTCTACCTTTTTCCAATTATAAGAACTGTTACTTTTT
CTCATTGTATGTTCTTTTAGATCAACTTGATGACTTAGTCTATGTTTTTTTGATATTTTACATT
GTAGTTTTCTGAGAGGAGATCAATATGCAATAGCATTGGCTTTGCTAGATGGAGGAAAAGTTGT
CCTGGGTGTGCTGGCTTGTCCAAATCTTCCACTAACTTCCCTCAGTGATGCTGGTCAGCATTCT
CCAAATAATAAAGTTGGCTGCCTTTTCTTTGCTGTAGTAGGTGGTGGAACTTATATGCAGCCAC
TTGATGGTTCTTCGGCAGTAAAGGTTATAATGATTTATTATAATTTTTTGCTATTAATCTCAGT
ATGTATCTGCAACTTGTACAATTATATGGGATCTCCAGGTGCAAGTAAGTGCTGTTGAAAATCC
TGAAGAAGCATCATTCTTTGAGTCTTATGAAGCAGCACACTCCATGCATGATTTATCTAGCTTG
ATTGCCCAAGTACTCTCCCTCTTGCTTTCTCTACATAGATATAAATAGGTAAAAGTATCATGGA
GGTCCCTGTATTAGGAGTCAGATTGTATTTTGCCCCCTCTACTCTAAAAATGGGCAAATTAATC
CTTATACGTTAGATCAAAGAGCAAACTAGTCTTTTTAGTTAAAAATTTCATCCATTTGTAGTCT
TAAAAAGTGGTGTGGTTCATAGAATAATTAGATAGTGACACATGGCATGTCACATGTACATCAT
ACTGATGTATATGGATCAGTTTTTAACAGTAAAAATGAATGGAATTTATAACAGAAAGACTAAT
TTGCTTTTTGATCTAACATACATGACTAATTTGCCTATTTTTTGAGTAAAGGGAGCAAAATACA
ATCCAACACCTAATACATGGCCTCCATGGTACTTTTACCAATATAAATATAATACATATATATA
TACACACACTAACGCTTTTGGATAATGTATTGAAAACGTTGAGATGTAAACAGAAACTCGGCGT
CAAAGCACCACCGGTTAGAATTGATAGCCAGGCGAAGTATGGTGCTCTATCCAGAGGAGATGGA
GCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAATATGGGATCATGCTGCTGGGT
GTATTGTTGTGAGTG
						
					

			
		

Sequences
Splice junction sequence	GCTCTATCCAGAGGAGATGGAGCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAA TATGGGATCATGCTGCTGGGTGTATTGTTGTGAGTGgctcacaagcactggttagttttgtgct gcagcaggaatttcctgataacttctcattagttgctgaggaggtgaatctctgtacttgtgaa taatagtt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 738 Transcript exons: 1038608-1038678,1038789-1038991,1039508-1039718,1039798- 1039896,1040389-1040542 GCTCACAAGCACTGGTTAGTTTTGTGCTGCAGCAGGAATTTCCTGATAACTTCTCATTAGTTGC TGAGGAGGATTCTAAAGATCTTCGCAAGGATGGTGGCCAGGAAATAGTAGAGCGCATTACAAAA CTTGTGAACGATTCTCTAACTATTGATGGATCATACAATGTTACTTTATCCACAGAAGATATTC TCAGGGCCATTGACAATGGCAGATCTGAAGGTGGTTCCCAAGGTCGACACTGGGTTTTGGATCC TATAGATGGTACTAAAGGTTTTCTGAGAGGAGATCAATATGCAATAGCATTGGCTTTGCTAGAT GGAGGAAAAGTTGTCCTGGGTGTGCTGGCTTGTCCAAATCTTCCACTAACTTCCCTCAGTGATG CTGGTCAGCATTCTCCAAATAATAAAGTTGGCTGCCTTTTCTTTGCTGTAGTAGGTGGTGGAAC TTATATGCAGCCACTTGATGGTTCTTCGGCAGTAAAGGTGCAAGTAAGTGCTGTTGAAAATCCT GAAGAAGCATCATTCTTTGAGTCTTATGAAGCAGCACACTCCATGCATGATTTATCTAGCTTGA TTGCCCAAAAACTCGGCGTCAAAGCACCACCGGTTAGAATTGATAGCCAGGCGAAGTATGGTGC TCTATCCAGAGGAGATGGAGCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAATA TGGGATCATGCTGCTGGGTGTATTGTTGTGAGTG
Genomic sequence	GCTCACAAGCACTGGTTAGTTTTGTGCTGCAGCAGGAATTTCCTGATAACTTCTCATTAGTTGC TGAGGAGGTGAATCTCTGTACTTGTGAATAATAGTTTCAGCAGCATGCATCAGCCATCTTTTTT TTACATCATAGAATTCTTCTTTCCTTCTAAATAATGTTTTGCTTTCTCTTTAGGATTCTAAAGA TCTTCGCAAGGATGGTGGCCAGGAAATAGTAGAGCGCATTACAAAACTTGTGAACGATTCTCTA ACTATTGATGGATCATACAATGTTACTTTATCCACAGAAGATATTCTCAGGGCCATTGACAATG GCAGATCTGAAGGTGGTTCCCAAGGTCGACACTGGGTTTTGGATCCTATAGATGGTACTAAAGG GTGAGCTCTGAAACTTTATATTTGACATAAGTGATTTTGCTTTAGTCATAAAGAAGTTGGTGTC TGCTATAATAATGATTCATGTGCACCGAGTGCATGTTTATGATATGCCTTATCCAACATGAGGT AGTAACTTTCCTTTTTACTCCTTAACTCAGCAATGACTACTGCAGGGGGATGTTTAGGGGCATA CCTTAACAGTACTATATGCAAGACAAACCATAATTCTGTCTTGGTCTTGCATAGCCAGGCCTTT GAATTTAGTGCTTTAGAATTAAATGACTGATCATCAACGAACTTTCTCATGTATATAACTTTTG TTTTATCTTTGTTACACATGTTATGTTTGGATTTCTTTGCAATTTAGTGTGAAAGAAATGGCTG ATCTTTTTATTTTTGCATAATAATGATCTCTACCTTTTTCCAATTATAAGAACTGTTACTTTTT CTCATTGTATGTTCTTTTAGATCAACTTGATGACTTAGTCTATGTTTTTTTGATATTTTACATT GTAGTTTTCTGAGAGGAGATCAATATGCAATAGCATTGGCTTTGCTAGATGGAGGAAAAGTTGT CCTGGGTGTGCTGGCTTGTCCAAATCTTCCACTAACTTCCCTCAGTGATGCTGGTCAGCATTCT CCAAATAATAAAGTTGGCTGCCTTTTCTTTGCTGTAGTAGGTGGTGGAACTTATATGCAGCCAC TTGATGGTTCTTCGGCAGTAAAGGTTATAATGATTTATTATAATTTTTTGCTATTAATCTCAGT ATGTATCTGCAACTTGTACAATTATATGGGATCTCCAGGTGCAAGTAAGTGCTGTTGAAAATCC TGAAGAAGCATCATTCTTTGAGTCTTATGAAGCAGCACACTCCATGCATGATTTATCTAGCTTG ATTGCCCAAGTACTCTCCCTCTTGCTTTCTCTACATAGATATAAATAGGTAAAAGTATCATGGA GGTCCCTGTATTAGGAGTCAGATTGTATTTTGCCCCCTCTACTCTAAAAATGGGCAAATTAATC CTTATACGTTAGATCAAAGAGCAAACTAGTCTTTTTAGTTAAAAATTTCATCCATTTGTAGTCT TAAAAAGTGGTGTGGTTCATAGAATAATTAGATAGTGACACATGGCATGTCACATGTACATCAT ACTGATGTATATGGATCAGTTTTTAACAGTAAAAATGAATGGAATTTATAACAGAAAGACTAAT TTGCTTTTTGATCTAACATACATGACTAATTTGCCTATTTTTTGAGTAAAGGGAGCAAAATACA ATCCAACACCTAATACATGGCCTCCATGGTACTTTTACCAATATAAATATAATACATATATATA TACACACACTAACGCTTTTGGATAATGTATTGAAAACGTTGAGATGTAAACAGAAACTCGGCGT CAAAGCACCACCGGTTAGAATTGATAGCCAGGCGAAGTATGGTGCTCTATCCAGAGGAGATGGA GCCATATATCTGCGTCTTCCACACAAAGGGTATCGAGAAAAAATATGGGATCATGCTGCTGGGT GTATTGTTGTGAGTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.790643941

Conservation Information
Conserved circRNAs	NA
PMCS	0.790643941

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1039867-1039814(+)
1038920-1040516(-)
						
						Potential amino acid sequence
						
							MHDLSSLIAQKLGVKAPPVRIDSQAKYGALSRGDGAIYLRLPHKGYREKIWDHAAGCIVVSGSQ
ALVSFVLQQEFPDNFSLVAEEDSKDLRKDGGQEIVERITKLVNDSLTIDGSYNVTLSTEDILRA
IDNGRSEGGSQGRHWVLDPIDGTKGFLRGDQYAIALALLDGGKVVLGVLACPNLPLTSLSDAGQ
HSPNNKVGCLFFAVVGGGTYMQPLDGSSAVKVIMIYYNFLLLISVCICNLYNYMGSPGASKCC*
(+)
MALRISSVDKVTLYDPSIVRESFTSFVMRSTISWPPSLRRSLESSSATNEKLSGNSCCSTKLTS
ACEPLTTIHPAA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1039867-1039814(+) 1038920-1040516(-)
Potential amino acid sequence	MHDLSSLIAQKLGVKAPPVRIDSQAKYGALSRGDGAIYLRLPHKGYREKIWDHAAGCIVVSGSQ ALVSFVLQQEFPDNFSLVAEEDSKDLRKDGGQEIVERITKLVNDSLTIDGSYNVTLSTEDILRA IDNGRSEGGSQGRHWVLDPIDGTKGFLRGDQYAIALALLDGGKVVLGVLACPNLPLTSLSDAGQ HSPNNKVGCLFFAVVGGGTYMQPLDGSSAVKVIMIYYNFLLLISVCICNLYNYMGSPGASKCC* (+) MALRISSVDKVTLYDPSIVRESFTSFVMRSTISWPPSLRRSLESSSATNEKLSGNSCCSTKLTS ACEPLTTIHPAA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.006G005300_circ_g.1

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